科研日报 2026-08-31
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📅 Daily Report - 2026-08-31
今日筛选出 2 条内容，来自 2 个来源
🤖 今日AI智能总结
🧬 数据前沿
今日焦点： 开发出一种新型高灵敏度转录组学方法（LCM-FFPEseq），首次实现了对存档的石蜡包埋（FFPE）组织切片进行单细胞分辨率的转录组测序。
主要方向：
- 利用激光捕获显微切割（LCM）技术，从有限的FFPE组织样本中获取高质量RNA。
- 实现对存档FFPE组织切片的单细胞分辨率转录组测序，揭示细胞异质性。
- 克服FFPE组织RNA降解的挑战，提高转录组测序的灵敏度和准确性。
技术亮点：
- LCM-FFPEseq：一种结合激光捕获显微切割和FFPE组织RNA提取与测序的新型技术平台。
- 单细胞分辨率：首次实现对FFPE组织进行单细胞级别的转录组分析。
🧪 博客更新
今日焦点： 首次发现，睡眠中频繁的微觉醒与阿尔茨海默病（AD）的遗传风险升高相关，为早期预警提供了新线索。
主要方向：
- 探索睡眠微觉醒作为AD无症状期早期生物标志物的潜力。
- 研究睡眠质量与AD遗传易感性之间的关联机制。
技术亮点：
- 利用多导睡眠图（PSG）技术，量化分析健康中年成人睡眠中的微觉醒频率。
- 结合AD相关基因检测，建立睡眠特征与遗传风险的关联模型。
📚 分类浏览
🧬 数据前沿 (1条)
详细内容（全部1条）
1. GSE305348 一种基于激光捕获显微切割的高灵敏度转录组学方法，利用LCM-FFPEseq技术从存档的FFPE组织切片中获得单细胞分辨率数据
- ✍️ 作者：未知作者
- 🏷️ 关键词：single-cell、transcriptomics
- 📝 描述：Contributors : Elise Callens ; Hannes Syryn ; Koen Deserranno ; Guillaume Cattebeke ; Henri Couckuyt ; Julie Van De Velde ; Danique Berrevoet ; Aaron Verplancke ; Dieter Deforce ; Ward De Spiegelaere ; Koen Van de Vijver ; Martine Cools ; Filip Van NieuwerburghSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensUnderstanding gene expression within its spatial context is essential for unravelling biological processes. Laser Capture Microdissection (LCM) has emerged as a transformative technology, enabling targeted isolation of individual cells or regions from tissue sections while preserving spatial context. However, its application to formalin-fixed, paraffin-embedded (FFPE) tissues has been limited by RNA degradation, leaving the vast repository of clinical FFPE samples underutilized. To address this, we introduce LCM-FFPEseq, a novel method combining LCM with the advanced Smart-seq3xpress protocol and FFPE-specific adaptations for spatial transcriptomics of FFPE sections. Unlike traditional protocols requiring thousands of cells to generate high-quality libraries, LCM-FFPEseq achieves high sensitivity, reproducibility, and transcript coverage. With as few as 30 FFPE-embedded K562 cells and Sertoli cells, we detected over 14,000 protein-coding genes per sample, with no substantial gains when a higher number of cells were isolated. Even individual LCM-isolated cells yielded an average of 7,353 or 6,490 protein-coding genes per K562 or Sertoli single cell, respectively. To demonstrate its clinical utility, we applied LCM-FFPEseq to archived testicular FFPE samples from transgender females receiving gender-affirming hormone therapy. Transcriptomic profiling of isolated seminiferous tubules revealed tubular hyalinization to be associated with greater upregulation of extracellular matrix remodelling and inflammatory pathways, alongside stronger downregulation of spermatogenesis-associated pathways. These findings suggest that testicular fibrosis and/or tubular hyalinization may contribute to germ cell loss following inappropriate hormonal exposure. By enabling high-resolution transcriptomics in archived FFPE samples, LCM-FFPEseq unlocks new possibilities for investigating rare cell types, spatial heterogeneity, and therapy-induced tissue remodelling in vast FFPE repositories.
- 🔗 查看原文
🧪 博客更新 (1条)
详细内容（全部1条）
1. 你的睡眠可能隐藏着阿尔茨海默病的早期迹象。
- ✍️ 作者：未知作者
- 🏷️ 关键词：Alzheimer
- 📝 描述：Frequent micro-awakenings during sleep were linked to higher genetic risk for Alzheimer’s in healthy middle-aged adults who had no symptoms. The discovery raises the possibility that subtle changes during sleep could offer an early warning signal years before memory problems appear.
- 🔗 查看原文
📊 关键词统计
| 关键词 | 出现次数 |
|---|---|
| Alzheimer | 1 |
| single-cell | 1 |
| transcriptomics | 1 |
📅 报告生成时间：2026-08-30 23:37
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