详细内容（全部7条）

1. GSE344397 SNORD11 破坏 BMSC 谱系稳态并增强与年龄相关的骨质疏松症中的巨噬细胞炎症

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、inflammation
• 📝 描述：Contributors : Saishuai Yang ; Guang Yang ; Liangrui Li ; Mingjie Xia ; Jiale Huang ; Zhiming Cui ; Jiajia ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusFor transcriptome profiling, total RNA from control and SNORD11-manipulated BMSCs was submitted to sequencing. Libraries were prepared using a poly(A)-enrichment strategy. Clean reads were aligned to the reference genome using HISAT2, and gene expression was quantified as raw counts.
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2. GSE329447 巨噬细胞中 Piezo1 依赖性神经免疫信号传导驱动结肠炎相关内脏疼痛 [scRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、scRNA
• 📝 描述：Contributors : Liyan Wu ; Tianyi Shen ; Zihan Wang ; Ting Wang ; Moubin Lin ; Jing Feng ; Heng XuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusVisceral pain is a hallmark symptom of ulcerative colitis, yet its underlying mechanisms remain poorly defined. While neuro-immune interactions have been implicated in somatic pain, the contribution of specific immune cell subsets to colitis-associated visceral hypersensitivity is unclear. Here, we identify the mechanosensitive ion channel Piezo1 in CX3CR1+ resident macrophages as a key regulator of visceral pain in a murine model of dextran sulfate sodium (DSS)-induced colitis. Single-cell RNA sequencing and functional assays revealed that Piezo1 is upregulated and functionally active in both resident and infiltrating macrophages during colitis. However, conditional deletion of Piezo1 in CX3CR1+ resident macrophages, but not in CCR2+ infiltrating macrophages, markedly attenuated visceral hypersensitivity and alleviated colonic inflammation. Mechanistically, Piezo1 activation induced NGF expression in resident macrophages, which engaged TrkA signaling to promote sensory nerve remodeling and visceral pain. Genetic ablation of NGF in CX3CR1+ macrophages or TrkA in TRPV1+ sensory neurons recapitulated the analgesic effects. Together, these findings define a mechanosensory neuro-immune axis wherein Piezo1-expressing resident macrophage promotes nociceptor sensitization via NGF-TrkA signaling, highlighting Piezo1 as a potential therapeutic target for visceral pain in colitis.
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3. GSE307893 小鼠 MASH 肝脏和健康对照的单细胞 RNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、scRNA
• 📝 描述：Contributors : Ziniu Ding ; Tao LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMASH is rapidly becoming a leading cause of chronic liver disease worldwide, with its prevalence soaring in parallel with the global epidemics of obesity and type 2 diabetes. The progression of MASH is driven by a complex network of cellular crosstalk involving dysfunctional hepatocytes, activated immune cells, and fibrogenic hepatic stellate cells, whose maladaptive communication orchestrates inflammation and fibrosis. We performed single-cell RNA sequencing to characterize the transcriptomes of MASH and normal liver tissues from mice at the single cell level using the BGI C4 single-cell platform.
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4. GSE343072 利用 CAGE-Seq 对衰老人类骨骼肌中的基因表达进行分子表型分析和启动子特异性调控

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Contributors : Alina V Erina ; Andrey I Buyan ; Ivan V Kulakovskiy ; Oleg A Gusev ; Guzel R Gazizova ; Elena I Shagimardanova ; Yulia P Sergeeva ; Leyla K Shigapova ; Anastasia Y Efimenko ; Daniill V PopovSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn this study, we applied cap analysis of gene expression (CAGE-Seq) to biopsy samples of the vastus lateralis muscle and quantitatively profiled the activity of thousands of transcribed regulatory elements. The collection of samples was previously described and partially profiled with RNA-Seq (https://doi.org/10.1111/acel.14098 and https://doi.org/10.1134/S0006297924020093).
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5. GSE310086 乳酸通过改变染色质可及性驱动肝星状细胞的表观遗传重编程和激活

• ✍️ 作者：未知作者
• 🏷️ 关键词：epigenetic
• 📝 描述：Contributors : Ziniu Ding ; Tao LiSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensActivation of hepatic stellate cells (HSCs) is a central driver of liver fibrosis, and the metabolite lactate has emerged as a potential signaling molecule beyond its role in metabolism. To investigate whether it influences HSC activation through epigenetic mechanisms, we treated human primary HSCs with L-lactate and utilized Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) to map genome-wide changes in chromatin accessibility, complemented by assays for gene expression to correlate these changes with the pro-fibrotic activation phenotype.
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6. GSE304448 巨噬细胞中 Piezo1 依赖性神经免疫信号传导驱动结肠炎相关内脏疼痛

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune
• 📝 描述：Contributors : Liyan Wu ; Tianyi Shen ; Zihan Wang ; Ting Wang ; Moubin Lin ; Jing Feng ; Heng XuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusVisceral pain is a hallmark symptom of ulcerative colitis, yet its underlying mechanisms remain poorly defined. While neuro-immune interactions have been implicated in somatic pain, the contribution of specific immune cell subsets to colitis-associated visceral hypersensitivity is unclear. Here, we identify the mechanosensitive ion channel Piezo1 in CX3CR1+ resident macrophages as a key regulator of visceral pain in a murine model of dextran sulfate sodium (DSS)-induced colitis. Single-cell RNA sequencing and functional assays revealed that Piezo1 is upregulated and functionally active in both resident and infiltrating macrophages during colitis. However, conditional deletion of Piezo1 in CX3CR1+ resident macrophages, but not in CCR2+ infiltrating macrophages, markedly attenuated visceral hypersensitivity and alleviated colonic inflammation. Mechanistically, Piezo1 activation induced NGF expression in resident macrophages, which engaged TrkA signaling to promote sensory nerve remodeling and visceral pain. Genetic ablation of NGF in CX3CR1+ macrophages or TrkA in TRPV1+ sensory neurons recapitulated the analgesic effects. Together, these findings define a mechanosensory neuro-immune axis wherein Piezo1-expressing resident macrophage promotes nociceptor sensitization via NGF-TrkA signaling, highlighting Piezo1 as a potential therapeutic target for visceral pain in colitis.
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7. GSE306408 靶向 miR-146a 和 miR-21 的 AntagomiR 可诱导大细胞转化型蕈样肉芽肿肿瘤细胞死亡并恢复 CD8+ T 细胞功能

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor
• 📝 描述：Contributors : Christiane Querfeld ; Zhen Han ; Piotr Swiderski ; Xiwei WuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensLarge cell transformation in mycosis fungoides (LCT-MF) occurs in 20-50% of patients with advanced MF, the most common subtype of cutaneous T cell lymphoma (CTCL) and is associated with an aggressive clinical course and poor survival. We previously identified a distinct microRNA (miR) expression profile in LCT-MF with upregulation of miR-21 and miR-146a compared to non-LCT MF. Here, we investigated the efficacy of 2 novel synthesized and chemically modified antagomiRs amiR-146a, and amiR-21 linked to CpG–D19 for delivery on both tumor growth and T cell exhaustion. Targeting miR-146 and miR-21 alone or combined induced apoptosis through activated caspase 3/7 pathways and caused cell cycle arrest at the G2/M phase via the STAT3/CDK1/Cyclin B1 pathway in CTCL cell lines. In vivo, treatment reduced tumor growth and prolonged survival in mouse xenograft models. CD8+ T cells from LCT-MF patient biopsies exhibited upregulation of immune checkpoint proteins and activation of the STAT3/IRF4/BATF pathway, similar to exhausted CD8+ T cells from healthy donors stimulated to exhaustion in a CTCL culture model. Treatment of these exhausted CD8+ T cells with amiR-146a and amiR-21 alone or combined attenuated their exhaustion phenotype through decreased immune checkpoint expression, and enhanced cytokine production by inhibiting the STAT3/IRF4/BATF pathway. Collectively, our findings indicate that targeting miR-146a and miR-21 offers a promising new therapeutic approach for LCT-MF, by both suppressing tumor growth and restoring T cell function to enhance anti-tumor immunity.
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详细内容（全部3条）

1. ⭐ 这种肠道微生物可能有助于你随着年龄增长保持强健体魄。

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:micro(b|be|bial|organism)、gut、regex:gut(-?microbiome)?
• 📝 描述：Scientists have linked a specific gut bacterium, Roseburia inulinivorans, to stronger muscles in both humans and mice. Older adults who carried it had 29% greater handgrip strength, while treated mice gained about 30% more grip strength and developed larger, more fast-twitch muscle fibers. The bacterium was also less common in older people. Researchers say it could eventually become a probiotic candidate for helping combat age-related muscle loss.
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2. 这种酵母补充剂可能增强抗癌免疫力。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immunity
• 📝 描述：A simple yeast-based food supplement may help restore the immune system’s ability to fight cancer, according to new research in mice. Scientists found that yeast beta-glucan reprogrammed early immune cells in the bone marrow, leading to stronger, longer-lasting cancer-fighting responses against colorectal, skin, and breast cancer cells.
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3. 牙齿数量可能预测胰腺癌手术后的生存率

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Pancreatic cancer patients with at least 21 natural teeth survived nearly two years longer after surgery than those with fewer teeth in a study of 339 people. Researchers suspect tooth loss may reflect decades of inflammation, frailty, nutrition, and other factors tied to the body’s overall resilience.
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