详细内容（前10条）

1. GSE342172 靶向多胺代谢和eIF5A次黄嘌呤化治疗儿童急性髓系白血病

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、metabolism
• 📝 描述：Contributors : Courtney Jones ; Nathan SalomonisSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensPediatric acute myeloid leukemia (AML) is a devasting disease with 5-year survival outcomes of approximately 60%. Therefore, there is an urgent need to develop novel therapies for pediatric AML patients. Numerous studies have demonstrated that cellular metabolism is a key vulnerability in adult AML; however, we understand very little about metabolism in pediatric AML. Using pediatric AML patient derived xenograft models, we demonstrate that polyamine metabolism is a pharmacologically targetable vulnerability of pediatric AML cells. Further, depletion of polyamines sensitizes pediatric AML cells to chemotherapy in vitro and in vivo. Mechanistically, polyamine depletion causes reduced protein synthesis through decreased hypusination of translational elongation factor eIF5A. Pharmacologic polyamine depletion and inhibition of eIF5A hypusination result in reduced protein synthesis and decreased expression of cell cycle regulatory proteins resulting in a G1 cell cycle arrest and eventual cell death through apoptosis. In summary, this study reveals polyamine metabolism and hypusination of eIF5A as a vulnerability in pediatric AML that can be targeted using clinically trackable approaches.
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2. GSE341740 GAS6 驱动卵巢癌的化疗耐药性和免疫逃逸

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHigh-grade serous ovarian cancer (HGSOC) has poor prognosis owing to widespread platinum resistance and unsatisfactory efficacy of chemo-immunotherapy, so novel mechanisms of therapeutic failure are urgently required. The GAS6/AXL axis supports tumor survival and immunosuppression, yet how chemotherapy modulates GAS6 expression remains unclear. We demonstrate that chemotherapy elevates GAS6, which activates AXL to trigger NF-κB-mediated transcription of GDF-15. This GAS6-AXL-GDF-15 cascade drives intrinsic platinum resistance and tumor immune evasion. Blocking GDF-15 reverses chemoresistance; combined with anti-PD-1 antibody, it produces synergistic anti-tumor effects. Circulating GAS6 protein reflects activation of this signaling cascade and acts as a non-invasive predictive biomarker for combined GDF-15/PD-1 blockade. Collectively, we identify a chemotherapy-activated GAS6/AXL-NF-κB-GDF-15 pathway that mediates both chemoresistance and immune escape in HGSOC, supporting translational application of GDF-15 inhibitors plus immune checkpoint inhibitors for biomarker-stratified patients.
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3. GSE338857 对从接受TAC手术的REFLEX小鼠中分离的GFP阳性和GFP阴性CD45阳性和CD45阴性心肌细胞进行单细胞转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：cardiac、single-cell
• 📝 描述：Contributors : Tatsuyuki Sato ; Takayuki IsagawaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCardiac remodeling induced by pressure overload is accompanied by extensive changes in intercellular communication among cardiac cell populations. To identify cells that physically interact with vascular endothelial cells in vivo, we utilized the REFLEX system combined with HUNTERuni-seq, a single-cell transcriptomic approach for profiling interacting cell populations. Cdh5-CreERT2;REFLEX mice were subjected to transverse aortic constriction (TAC), and GFP-positive cells representing endothelial-interacting cells were isolated from the heart. Single-cell RNA sequencing was performed to characterize the cellular composition and transcriptional states of interacting and non-interacting cell populations in the pressure-overloaded myocardium. These data provide a resource for investigating endothelial-centered cellular interaction networks during cardiac remodeling.
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4. GSE335342 Meteorin样蛋白通过调节巨噬细胞调控心肌梗死后的心脏修复

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、cardiac
• 📝 描述：Contributors : Gemma Ferrer-Curriu ; Albert Blasco-Roset ; Artur Navarro-Gascon ; Carolina Soler-Botija ; Francisco Javier Godoy-Nieto ; Marta Monguió-Tortajada ; Rubén Cereijo ; Tania Quesada-López ; F Rueda ; Santiago Roura ; Carolina Gálvez-Montón ; Francesc Villarroya ; Antoni Bayés-Genís ; Anna PlanavilaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAfter myocardial infarction (MI), the heart undergoes a reparative process that includes an initial acute inflammatory phase followed by a subsequent reparative phase. The transition between these phases is crucial for cardiac recovery, but the key factors remainunclear. Meteorin-like (Metrnl) promotes anti-inflammatory macrophage polarization in the myocardium, yet its role in the acute phase post-MI is unknown. We observed that macrophages infiltrating the ischemic myocardium produced elevated levels of Metrnl inboth the heart and circulation 4 days post-MI. The absence of Metrnl in Metrnl⁻/⁻ mice altered myocardial healing and remodeling, with an increased presence of macrophages with a more pro-inflammatory phenotype. Conversely, cardiac Metrnl overexpressionrestored myocardial repair and promoted a shift toward a more anti-inflammatory macrophage phenotype. Mechanistically, Metrnl regulated macrophage-dependent production of Oncostatin M (Osm), a key cytokine in the early inflammatory phase post-MI that induces cardiomyocyte production of Reg3β. Reg3β, in turn, limits classical macrophage activation and polarization while modulating their trafficking, ultimately influencing the duration and intensity of the pro-inflammatory phase post-MI. Thus,Metrnl plays a crucial role in cardiac repair by modulating the acute phase following myocardial infarction through its regulation of macrophage populations.
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5. GSE314205 利用新一代测序技术评估肠上皮细胞中 AhR 对肠道的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、regex:intestin(e|al)
• 📝 描述：Contributors : Han Jin ; Emiel P Van der VorstSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn this study, Ahr fl/fl control mice and AhR fl/fl Villin Cre+ mice were injected with PCSK9 overexpressing AAV8 particles and placed on a HFD for 12 weeks. Intestines from these mice were used to perform NGS analysis.
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6. GSE286966 深度学习发现 Pbx1 是造血干细胞衰老的网络枢纽 [scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、scRNA
• 📝 描述：Contributors : Hiroshi Kobayashi ; Yusuke Shiozawa ; Seishi Ogawa ; Keiyo TakuboSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHematopoietic stem cells (HSCs) constitute a well-structured hematopoietic system that undergoes an age-related bias toward myeloid/platelet differentiation. The mechanism by which this aging hallmark is deeply embedded in intrinsic HSC programs is unknown. Using single-cell RNA-seq data, old HSCs harbor two distinct transcriptional programs: one shared with megakaryocytes and the other associated with the most primitive HSCs. By employing a transformer-based model that captures higher-order differences between young and aging HSCs and integrating time-series transcriptomic analyses, epigenetic studies, and comprehensive transcription factor screens, we identified Pbx1 as a central gene controlling both age-related transcriptional programs and differentiation defects in old HSCs. Pbx1 suppresses erythroid differentiation by repressing Gata1 expression. In addition, we found that the histone methyltransferase G9a/GLP acts as an epigenetic regulator that specifically inhibits Gata1 expression and erythroid differentiation. These findings provide insight into the differentiation defects associated with stem cell aging and potential strategies for rejuvenation.
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7. GSE341840 整合转录组学、孟德尔随机化、机器学习和实验验证以识别伴有甲状腺功能障碍的脓毒症的特征基因_验证队列

• ✍️ 作者：未知作者
• 🏷️ 关键词：transcriptomics
• 📝 描述：Contributor : Li YeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSepsis is frequently associated with thyroid dysfunction. However, the underlying molecular mechanisms and effective biomarkers for sepsis with thyroid dysfunction remain unclear.
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8. GSE341837 整合转录组学、孟德尔随机化、机器学习和实验验证以识别伴有甲状腺功能障碍的脓毒症中的特征基因_发现队列

• ✍️ 作者：未知作者
• 🏷️ 关键词：transcriptomics
• 📝 描述：Contributor : Li YeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSepsis is frequently associated with thyroid dysfunction. However, the underlying molecular mechanisms and effective biomarkers for sepsis with thyroid dysfunction remain unclear.
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9. GSE341811 Pleckstrin-2 通过将 Profilin-1 募集到细胞膜上以破坏胞质 PTEN 的稳定性来促进皮肤鳞状细胞癌的发生

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma
• 📝 描述：Contributors : Guanfei Zhang ; Meng Liu ; Lanlan Cui ; Zongguan Huang ; Mo Zhang ; Fengyun Sun ; Zhenzhen Lu ; Changwei Hu ; Xiaofei Zhao ; Yiming Hu ; Ying Li ; Shujun Han ; Yan Zheng ; Yongping ShaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCutaneous squamous cell carcinoma (cSCC) is a prevalent skin malignancy driven by ultraviolet-induced genomic damage, and advanced disease remains clinically challenging due to limited therapeutic options. Through integrative transcriptomic and functional analyses, we identify Pleckstrin-2 (PLEK2) as a previously unrecognized oncogenic driver in cSCC. PLEK2 is markedly upregulated in patient tumors, cSCC cell lines, and UV-induced mouse models. Genetic perturbation studies demonstrate that PLEK2 promotes proliferation, migration, epithelial–mesenchymal transition, and tumorigenicity. Mechanistically, PLEK2 recruits Profilin-1 (PFN1) to the plasma membrane through its DEP domain, thereby reducing cytosolic PFN1 pool required for PTEN stabilization. This PFN1 sequestration accelerates PTEN polyubiquitination and degradation, thereby sustaining AKT pathway activation. Upstream, we identify ETS2 as a direct transcriptional activator of PLEK2, establishing an ETS2–PLEK2–PFN1–PTEN axis that drives cSCC progression. These findings delineate a signaling pathway regulating PTEN stability and AKT activation, and provide mechanistic insight into cSCC progression.
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10. GSE341769 RNA测序分析氯喹那醇处理的THP-1细胞

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Contributors : Runfa Chen ; Miaomiao Cai ; Yuting Dai ; Boyuan Shen ; Yinghong He ; Wen Zhang ; Zhijie Cui ; Jing Yang ; Zhengfan Jiang ; Xiang ZhouSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBased on our findings that chlorquinaldol can exert a potent antiviral effect both in vivo and in vitro, to explore the molecular mechanisms underlying chlorquinaldol-induced gene expression, we performed RNA sequencing analysis on THP-1 cells treated with either a low-concentration or a high-concentration of chlorquinaldol, using treatment of the STING agonist 3'3’-cGAMP as an antiviral control.
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1. 一项重要研究发现，减少蛋白质摄入量可能延缓衰老。

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：A sweeping review of more than 350 studies suggests that the protein boom may be overselling what many people actually need. Eating less protein appears to improve metabolism, reduce inflammation and cellular damage, and activate pathways linked to healthier aging and longer life.
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2. 癌细胞可能通过破坏自身DNA来维持生长。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Cancer cells rely on powerful genetic switches to keep growth genes running at full speed, but that intense activity can damage their own DNA. The resulting breaks are repeatedly repaired, sometimes with small mistakes that allow new mutations to accumulate. Researchers believe this self-inflicted damage may help tumors evolve while also creating a potential target for new treatments.
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• GSE339493 白细胞介素-33激活的2型固有淋巴细胞驱动子宫内膜异位症中的2型免疫功能障碍
• GSE338956 体内线粒体DNA转移动力学可诱导剂量依赖性肿瘤细胞去分化和异质性
• GSE304495 OCT4 负调控人腺病毒早期区域 3 免疫逃逸基因的转录程序
• GSE288397 整合机器学习和转录组学以提高酵母中β-胡萝卜素的产量
• GSE339483 卵巢癌唾液 miRNA
• GSE339436 CD97/ADGRE5 减弱过敏性哮喘中适应性 2 型免疫反应的诱导
• GSE325577 拟南芥 BR 调控的 ces-D 和 ces-qM 突变体的 DNA 甲基化谱分析 II
• GSE304970 鼹鼠基因表达进化的RNA测序分析
• GSE304334 拟南芥 BR 调控的 ces-D 和 ces-qM 突变体的 DNA 甲基化谱分析