详细内容（前10条）

1. ⭐ GSE313981 白细胞介素-7 受体通过 AKT 去磷酸化抑制 LCP1 来塑造缺血性卒中后的单核细胞-巨噬细胞异质性和脂质代谢 [scRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、monocyte、metabolism、scRNA
• 📝 描述：Contributors : Yan Wang ; Qianqian YinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn the present study, we performed single-cell RNA sequencing of immune cells isolated from the brain 72 hours after infarction, a time point at which peripheral monocyte infiltration peaks . Our analysis identified a monocyte population with high IL-7R expression, within which the macrophage subset exhibited the highest scores for fatty acid metabolism. Moreover, IL-7R knockdown reduced Akt phosphorylation and decreased LCP1 expression. Given our previous finding that LCP1 upregulation is associated with enhanced fatty acid metabolic programs, we next sought to dissect the cellular mechanisms through which LCP1 regulates monocyte lipid metabolism and polarization. To this end, we generated LCP1 knockdown cell lines and performed RNA sequencing and metabolomics analyses. Consistent with these in vitro findings, mice receiving IL-7R antibody treatment exhibited smaller infarct volumes and improved neurological scores. Together, these results provide mechanistic insight into how IL-7R–LCP1 signaling regulates immune cell metabolism in stroke-induced neuroinflammation and may inform future therapeutic strategies targeting immune metabolic pathways in ischemic stroke.
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2. ⭐ GSE208068 整合全基因组 DNA 甲基化和转录组分析将 EPHA5 列为口腔鳞状细胞癌的候选非侵入性表观遗传生物标志物

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、genome、epigenetic、methylation
• 📝 描述：Contributors : Semra Demokan ; Begüm ÖzemekSeries Type : Expression profiling by array ; Methylation profiling by arrayOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
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3. ⭐ GSE337519 肺癌新辅助化疗-免疫治疗反应的单细胞转录组图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、regex:immuno(logy|therapy|suppression)、single-cell
• 📝 描述：Contributors : Jian Li ; Zhouwenli Meng ; Ziming LiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis study provides a high-resolution single-cell transcriptional profile of lung cancer in the context of neoadjuvant treatment. Utilizing the 10x Genomics platform, we performed single-cell RNA sequencing (scRNA-seq) on paired patient samples to investigate the cellular composition, phenotypic states, and transcriptional dynamics of the tumor microenvironment in response to the therapeutic intervention.
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4. ⭐ GSE316860 TLR7 - ITGAM：心脏缺血损伤中的一种新型先天免疫通路 [snRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、cardiac、pathway
• 📝 描述：Contributors : Yijia Li ; Amol C Shetty ; Wei ChaoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe utilized single-nucleus RNA sequencing (sn-RNAseq) with the 10X Genomics platform to investigate the mechanisms underlying cardiac ischemia/reperfusion (I/R) injury. Cardiovascular disease (CVD), particularly ischemic myocardial infarction (MI), remains the leading cause of death in the U.S. Although percutaneous coronary intervention (PCI) reduces infarct size and preserves cardiac function, reperfusion itself induces I/R injury, exacerbating myocardial damage. Our prior studies demonstrate that I/R injury triggers the release of cellular RNA, and attenuating extracellular RNA (exRNA) reduces myocardial inflammation, conferring cardioprotection against I/R injury. This study elucidates the role of TLR7, an exRNA sensor, and its downstream signaling pathways in myocardial I/R injury, offering new insights into potential therapeutic strategies for cardiac I/R treatment.
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5. ⭐ GSE313837 白细胞介素-7受体通过AKT去磷酸化抑制LCP1，从而影响缺血性卒中后的单核细胞-巨噬细胞异质性和脂质代谢

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、monocyte、metabolism
• 📝 描述：Contributors : Yan Wang ; Qianqian YinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn the present study, we performed single-cell RNA sequencing of immune cells isolated from the brain 72 hours after infarction, a time point at which peripheral monocyte infiltration peaks . Our analysis identified a monocyte population with high IL-7R expression, within which the macrophage subset exhibited the highest scores for fatty acid metabolism. Moreover, IL-7R knockdown reduced Akt phosphorylation and decreased LCP1 expression. Given our previous finding that LCP1 upregulation is associated with enhanced fatty acid metabolic programs, we next sought to dissect the cellular mechanisms through which LCP1 regulates monocyte lipid metabolism and polarization. To this end, we generated LCP1 knockdown cell lines and performed RNA sequencing and metabolomics analyses. Consistent with these in vitro findings, mice receiving IL-7R antibody treatment exhibited smaller infarct volumes and improved neurological scores. Together, these results provide mechanistic insight into how IL-7R–LCP1 signaling regulates immune cell metabolism in stroke-induced neuroinflammation and may inform future therapeutic strategies targeting immune metabolic pathways in ischemic stroke.
• 🔗 查看原文

6. ⭐ GSE328634 积雪草中的积雪草酸可缓解肾纤维化：通过协调调节肠-肾轴和视黄醇代谢

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Liwei Zhu ; Ziyun Xu ; Xiaoyan Chang ; Jingyi Wu ; Zhenfang Du ; Haiyong Chen ; Sheng QiangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusChronic kidney disease (CKD) is a progressive condition characterized by renal fibrosis, with limited treatment options. Asiatic acid (AA), a triterpenoid derived from Centella asiatica, has shown renoprotective potential as a dietary supplement. This study integrated 16S rRNA sequencing, renal metabolomics, transcriptomics, and Metorigin analysis to investigate the renoprotective effects of AA in an adenine-induced CKD mouse model. AA significantly improved renal function, attenuated fibrosis, restored gut barrier integrity. Antibiotic depletion experiments demonstrated that an intact gut microbiota is required for the renoprotective effects of AA, supporting a functional link along the gut–kidney axis. AA modulated gut microbiota composition, notably enriching the beneficial genus Lachnospiraceae NK4A136 group. Multi-omics integration identified retinol metabolism as the key renal pathway rescued by AA, with metabolites including 9-cis-retinal and 4-hydroxyretinoic acid (4-OH-RA) showing strong negative correlations with renal injury markers.In vitro, 4-OH-RA suppressed fibrotic injury and oxidative stress in TGF-β1 stimulated tubular cells. These findings suggest that AA alleviates CKD in association with remodeling of the gut microbiota and reprogramming of renal retinol metabolism, highlighting its potential as a microbiota-targeting dietary supplement for renal protection., supporting its potential as a dietary supplement for countering renal fibrosis and supporting kidney health.
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7. ⭐ GSE341489 通过对同源人类 iPSC 克隆进行靶向测序揭示 CRISPR/Cas9 基因组编辑的隐藏结果 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、RNA-seq、genome
• 📝 描述：Contributors : Tubalova Maria ; Koksharova Galina ; Costa Silvia ; Krepischi Ana ; Fishman Veniamin ; Gridina MariaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCRISPR/Cas9 editing can create complex on-target alterations that escape conventional PCR genotyping. We characterized ten edited human induced pluripotent stem cell clones derived from four related parental lines carrying a tandem Xq25 duplication encompassing STAG2. Targeted genomic sequencing revealed inversions, plasmid integration, and complex rearrangements and resolved discrepancies between genotyping and transcriptomic observations. This GEO submission provides quantitative genome-browser coverage tracks supporting the transcriptomic and whole-genome analyses. BioProject: PRJNA1499378.
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8. ⭐ GSE303963 揭示鸡体内双重和三重 Toll 样受体 (TLR) 激动剂组合的免疫信号通路

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、TLR、Toll-like receptor
• 📝 描述：Contributors : Abinaya Kaliappan ; Saravanan Ramakrishnan ; Khushboo Panwar ; Prasad Thomas ; Naveen Dumala ; Surya K Varma ; Mithilesh Singh ; Sohini Dey ; Madhan M ChellappaSeries Type : Expression profiling by high throughput sequencingOrganism : Gallus gallusToll-like receptors (TLRs) are innate receptors playing key role in innate immunity and further shaping the adaptive immunity. Simultaneous activation of different TLRs results in cross-talk and can lead to either synergistic or antagonistic effects on the immune responses. Toll-like receptor (TLR) agonists are used as immunomodulatory agents and as vaccine adjuvants in human and chickens. Combinations of different TLR agonists are being explored to alleviate systemic toxicity and achieve long-lasting desirable immune responses. Dual (R-848/TLR7 and CpG-ODN/TLR21) and Triple (LPS/TLR4; R-848/TLR7 and CpG-ODN/TLR21) TLR agonists induced immune response in chicken were analysed through spleenic transcriptome. Our data, helps to understand the mechanistic basis and key molecules behind the dual/triple-TLR signalling in chicken, which can be tailored to achieve desired immune response and improve efficacy of therapeutic formulations.
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9. ⭐ GSE302356 通过单细胞多组学和空间转录组学的整合分析解码淋巴管肌瘤病 (LAM) 微环境

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Ken Chen ; Shuyang Zhao ; Minzhe Guo ; Hasan Reza ; Andrew Wagner ; Adnan Cihan Cakar ; Cheng Jiang ; Erik Zhang ; Jenna Green ; Emily Martin ; Kathryn Wikenheiser-Brokamp ; Anne K Perl ; Debora Sinner ; Jane Yu ; Yan XuSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencing ; OtherOrganism : Homo sapiensLymphangioleiomyomatosis (LAM) is a rare, progressive lung disease characterized by cystic destruction and metastatic growth of smooth muscle-like cells. Despite advances in understanding its genetic basis, the cellular heterogeneity, regulatory mechanisms, and microenvironmental interactions driving LAM progression remain poorly defined. We identified two spatially and functionally distinct LAM subtypes: LAMCORE1 and LAMCORE2. LAMCORE1 cells exhibited a uterine smooth muscle-like phenotype, expressing associated markers (ACTA2, MYH11) and were enriched in MTORC1 signaling and myogenic pathways, supporting a uterine origin. In contrast, LAMCORE2 cells displayed fibroblast-like features, with upregulated extracellular matrix (ECM) remodeling genes (COL1A1, MMP11) and epithelial-to-mesenchymal transition (EMT) pathways, suggesting a role in niche formation. Pseudotime and regulon analyses revealed dynamic transitions between these subtypes, driven by distinct transcriptional networks (e.g., HOX/PBX in LAMCORE1, TWIST/ZEB in LAMCORE2). The presence of the two distinct LAM subtypes was further validated by RNAscope and immunofluorescence microscopy.
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10. GSE326906 年轻和老年小鼠心脏骤停后脑组织的免疫图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、cardiac
• 📝 描述：Contributors : Wei Yang ; Ran Zhang ; Lihong Dang ; Vaibhav JainSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe objective of the current study was to profile immune cells in the post-cardiac arrest brain of young and aged mice at single-cell resolution.
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1. ⭐ Seq-Scope-X 将空间转录组学推向了超越光学分辨率的水平

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：RNA sequencing combined with tissue expansion enabled ultra-high-resolution spatial transcriptomics, allowing researchers to map gene activity within individual cells with…
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2. ⭐ 空间转录组学基准测试比较了领先的平台

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：RNA sequencing and spatial transcriptomics were combined to benchmark leading platforms, helping researchers compare how accurately each maps gene activity within complex tissues…
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3. 你的直觉或许能帮助你的大脑决定要记住什么。

• ✍️ 作者：未知作者
• 🏷️ 关键词：gut、regex:gut(-?microbiome)?
• 📝 描述：Signals traveling from the gut to the brain may help turn meals into lasting memories. Rats showed stronger memory activity after consuming nutritious foods, but not after drinking sweet liquids with little or no nutritional value. Blocking the vagus nerve erased this effect and made it harder for the animals to remember where food had been located. Over time, diets high in fat and sugar also weakened this memory system.
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• GSE316206 Mettl1 对小鼠骨髓细胞单细胞分辨率转录组的影响 [scRNA-seq]
• GSE313491 巨噬细胞衰老与 ACL 损伤和创伤后骨关节炎及肌肉萎缩相关 [scRNA-seq]
• GSE302465 TNF-α刺激的NCM460肠上皮细胞的RNA测序
• GSE299604 SMARCA4、STK11 和 KEAP1 共缺失与肺腺癌 (LUAD) 预后不良和 TGF-β 通路上调相关 - RNA-seq
• GSE253428 G422TN-GBM模型中第7天肿瘤的空间转录组测序
• GSE324689 黑热病后皮肤利什曼病 (PKDL) 全基因组转录谱分析（批量 RNA 测序）
• GSE286042 基于系统生物学的对全细胞和无细胞百日咳疫苗免疫反应的评估：组学工作流程的原理、方法和入组程序 [14-0015-转录组学]
• GSE341490 通过对同源人类iPSC克隆进行靶向测序揭示CRISPR/Cas9基因组编辑的隐藏结果
• GSE318902 线粒体 DNA 突变引发急性结肠炎期间早期肠上皮细胞功能障碍 [scRNA-Seq]
• GSE341563 在一项跨脑区和多队列研究中探究与路易体病理相关的DNA甲基化
• GSE339465 体外转录组学和分子动力学揭示巴马汀通过靶向 LCK 来共同调节 NF-κB 和 Wnt 信号通路，从而成为治疗银屑病的局部候选药物
• GSE336577 整合转录组学和免疫学分析揭示了需要有创机械通气的 COVID-19 ARDS 患者独特的线粒体和免疫特征
• GSE335667 预测阿尔茨海默病中相关的小胶质细胞间通讯通路：SPP1 的作用
• GSE331377 RNA测序分析鉴定出的在小鼠输尿管E12.5+18h培养物经LiCl处理后失调的转录本。
• GSE326452 小鼠闭合性颅脑损伤后脑组织的批量 RNA 测序：IL-1R1 信号通路的作用
• GSE325761 UBTD1 通过 TNF/AP-1 信号轴驱动卵巢癌进展和化疗耐药性
• GSE324499 KDM8 作为转录因子 SOX2 的共调节因子，促进细胞多能性 [ChIP-Seq]
• GSE324498 KDM8 作为转录因子 SOX2 的共调节因子，促进细胞多能性 [RNA-Seq]
• GSE316203 METTL1 对 AML 中整体基因表达的影响 [RNA-Seq]
• GSE313473 巨噬细胞衰老与前交叉韧带损伤、创伤后骨关节炎和肌肉萎缩相关
• GSE312205 剪接抑制通过破坏DNA修复能力和有丝分裂检查点完整性来阻碍胰腺癌细胞的存活。
• GSE308918 基于结构的微型 CRISPR-Cas12m 发现与工程改造用于表观基因组编辑
• GSE308905 基于结构的微型CRISPR-Cas12m表观基因组编辑的发现与工程改造
• GSE308788 HDAC4 通过组蛋白乙酰化介导 SMAD4 来调控 hASCs 的成骨和骨再生
• GSE308114 具有双锥形态和高聚集倾向的草酸钙晶体直接附着于乳腺癌细胞并影响其表型
• GSE301533 CD49d 通过肌动蛋白重排调控免疫突触形成，并同步 CLL 中的 BCR 信号传导
• GSE299788 基于结构的微型 CRISPR-Cas12m 发现与工程改造用于表观基因组编辑
• GSE295775 RNA-seq 针对 AML 细胞系在 ENO1 存在或不存在的情况下的情况
• GSE287496 CDK12 作为高危神经母细胞瘤联合治疗的切入点 [RNA-seq]
• GSE285409 ECD，一种新型的 U5 snRNA 结合蛋白，调控 RNA 剪接 [RNA-Seq, RIP-Seq]
• GSE281969 动态 STING 抑制在发育和成熟过程中协调免疫细胞功能。
• GSE280908 低强度脉冲超声调节破骨细胞线粒体功能治疗糖尿病牙周炎 [scRNA-seq]
• GSE266023 Nrf2介导的高渗反应基因染色质激活可减轻热诱导的肾损伤[RNA-seq]
• GSE266022 Nrf2 介导的高渗反应基因染色质激活可减轻热诱导的肾损伤 [ChIP-seq]
• GSE266021 Nrf2 介导的染色质激活高渗反应基因可减轻热诱导的肾损伤 [ATAC-seq]
• GSE223849 拟南芥高密度脂蛋白的全基因组结合图谱
• GSE337662 癌症相关成纤维细胞衍生的 CCL11 通过 CDK5 诱导上皮-间质转化促进前列腺癌恶性进展
• GSE336247 心脏成纤维细胞中 PTBP1 的缺失通过选择性剪接调节促纤维化反应 II
• GSE333833 父系补充蛋氨酸会改变绵羊植入前胚胎的DNA甲基化模式
• GSE330098 多组织微生理系统捕捉对营养过载和代谢治疗的综合反应
• GSE322892 新型疏水标签降解剂通过募集 HSP27 介导的 E3 连接酶复合物进行 ERα 蛋白酶体降解，从而克服内分泌耐药性乳腺癌
• GSE322796 BCMA CAR-T 细胞疗法相关肠炎中黏膜 CAR-T 细胞的持续存在和炎症重塑
• GSE320365 整合多组学分析揭示人类脂肪性肝炎肝细胞癌中新的基因-代谢物关系
• GSE317492 扁桃体类器官模型揭示了原发感染期间 Epstein-Barr 病毒感染的生发中心 B 细胞状态
• GSE316126 外周血单核细胞中独特的免疫代谢特征与2型糖尿病和糖尿病肾病中的全身炎症相关
• GSE305573 Ezh2 下调促进短寿命效应 CD8 T 细胞分化并削弱抗肿瘤免疫力
• GSE284191 溃疡性结肠炎发病机制阐明：以阑尾免疫为重点
• GSE252957 miR-34a-5p 处理的原代人血管内皮细胞的 RNA-seq 数据
• GSE194281 KDM1A 和 KDM7A 赖氨酸去甲基酶协同调节前列腺癌细胞中的雄激素受体功能
• GSE194280 KDM1A 和 KDM7A 赖氨酸去甲基酶协同调节前列腺癌细胞中的雄激素受体功能 [siKMD1A & siKDM7A]
• GSE194279 KDM1A 和 KDM7A 赖氨酸去甲基酶协同调节前列腺癌细胞中的雄激素受体功能 [KDM1A 和 KDM7A 抑制剂]
• GSE338031 脂肪细胞ILF3缺乏通过PUFA合成改善饮食诱导的肥胖和代谢紊乱
• GSE324959 胎盘 DNA 甲基化基因网络与野火相关的颗粒物暴露和儿童神经发育结果相关。
• GSE322854 早产和剖宫产影响新生儿CD4+ T细胞基因表达和细胞功能
• GSE320085 全基因组转录变化是斑马鱼逐渐且反复适应蛋白质营养不良的基础
• GSE315109 RACK1 通过直接结合和稳定 LDB1 来维持小鼠造血干细胞 [scRNA-Seq]
• GSE304109 RNA-seq 分析 SEM B-ALL 细胞中 KMT2A::AFF1 融合环状 RNA 的沉默情况。
• GSE303297 胰岛β细胞中Pdgfrα缺陷上调β细胞凋亡1并扰乱B6小鼠的葡萄糖代谢2
• GSE271386 SR30过表达转基因番茄叶片的RNA-seq数据
• GSE328751 Lenvatinib 治疗的 Tregs 在自身免疫性疾病中表现出优异的抗炎作用 [皮肤]
• GSE328467 研究表明，乐伐替尼治疗的调节性T细胞在自身免疫性疾病中表现出更优异的抗炎作用。
• GSE318907 线粒体 DNA 突变引发急性结肠炎期间早期肠上皮细胞功能障碍 [mtscATAC-seq]
• GSE318904 线粒体 DNA 突变引发急性结肠炎期间早期肠上皮细胞功能障碍 [scMultiome]