科研日报 2026-07-30

2026-07-30

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📅 Daily Report - 2026-07-30

今日筛选出 56 条内容，来自 2 个来源

🤖 今日AI智能总结

🧬 数据前沿

今日焦点：通过巨噬细胞导向的CAR-T细胞（GSE313197, GSE304568）实现肿瘤抗原独立的实体瘤靶向治疗，以及AKAP6 KBD肽在HFpEF猪模型中改善心脏功能（GSE335365, GSE335057）展现出重要临床潜力。

主要方向：

肿瘤免疫治疗：开发新型CAR-T细胞策略，通过巨噬细胞定向和肿瘤抗原独立机制靶向实体瘤；探索FcγR阻断增强免疫检查点治疗和克服耐药性。
心血管疾病研究：研究AKAP6 KBD肽对心力衰竭（HFpEF）猪模型心脏功能的改善作用。
神经发育障碍研究：大规模RNA测序分析多种自闭症谱系障碍（ASD）小鼠模型（GSE319614, GSE330728, GSE293709等）的海马体和前额叶皮层转录组，评估药物干预效果。
癌症机制探索：揭示SETD8介导的YAP单甲基化在结直肠癌发生中的抑制作用（GSE332607）。
免疫微环境调控：研究VHL基因丢失如何通过BMP10-CXCL13信号轴在肾脏淋巴管中诱导促肿瘤微环境（GSE308286）。

技术亮点：

多组学整合分析：结合单细胞RNA测序（scRNA-seq）和全基因组高通量测序技术，深入解析复杂生物系统。
大规模模型构建与分析：系统性地构建和分析17种ASD风险小鼠模型，为神经发育障碍研究提供丰富数据。

🧪 博客更新

今日焦点： RNA测序技术首次揭示了遗传因素如何影响癌症的演变和个体差异，并发现太空飞行扰乱生物钟可能增加癌症风险。

主要方向：

探究遗传背景对癌症发生、发展及治疗响应差异的影响。
研究太空飞行环境（辐射、生物钟紊乱）对肺部基因表达及癌症风险的潜在作用。
评估桑拿浴对免疫系统（白细胞数量）的短期激活效应。

技术亮点：

利用RNA测序技术深入解析癌症演变机制。

📚 分类浏览

🧬 数据前沿 (53条)

详细内容（前10条）

1. ⭐ GSE313197 Armored 1 巨噬细胞导向的抗 TREM2-CAR T 细胞对实体瘤的肿瘤抗原非依赖性靶向治疗 [人类 scRNA-seq]

✍️ 作者：未知作者
🏷️ 关键词：tumor、macrophage、antigen、scRNA
📝 描述：Contributors : Gal Yagel ; Dana Rimini ; Michelle von Locquenghien ; Roberto Avellino ; Pascale Zwicky ; Oren Barboy ; Gaya Granot ; Ken Xie ; Shir Shlomi-Loubaton ; Eyal David ; Kfir Mazuz ; Ido AmitSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensChimeric antigen receptor (CAR) T cell therapy has shown remarkable clinical efficacy in hematological malignancies and, more recently, in autoimmune diseases. However, its application in solid tumors has been limited by tumor antigen heterogeneity, antigen escape, and the immunosuppressive tumor microenvironment (TME), which is particularly shaped by tumor associated macrophages (TAMs). To overcome these barriers, we developed a novel CAR T cell strategy targeting human TREM2⁺ immunosuppressive TAMs. Macrophage-directed anti- TREM2-CAR T cells demonstrated potent anti-TREM2 activity in both in vitro assays and in solid tumor models in vivo. To further enhance intratumoral T cell function, we incorporated a CAR T cell responsive synthetic DNA biosensor comprising NFAT motifs that enable localized secretion of interleukin-12 upon CAR activation. In murine tumor models, this “armored” TREM2-targeted CAR-T cell approach led to robust remodeling of the TME and tumor-draining lymph nodes, characterized by TREM2⁺ TAM depletion, enhanced T and NK cell infiltration and activation, and complete tumor eradication, independent of tumor antigen expression.
🔗 查看原文

2. ⭐ GSE308286 VHL缺失通过BMP10诱导的CXCL13信号通路调控肾淋巴管中的促肿瘤微环境[RNA-Seq]

✍️ 作者：未知作者
🏷️ 关键词：tumor、tumor microenvironment、RNA-seq
📝 描述：Contributors : Tien Hsu ; Tuong-Vi Nguyen ; Hieu-Huy Nguyen-Tran ; Thi-Ngoc NguyenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusClear-cell renal cell carcinoma (ccRCC) is frequently associated with loss of the tumor suppressor gene VHL. To investigate the early events of ccRCC pathogenesis, we generated a conditional mouse model with Vhlh deletion in renal tubular epithelial cells. The model exhibited key histopathological features reminiscent of human ccRCC, including epithelial disorganization, immune infiltration, and vascular remodeling. VhlhloxP/loxP mice were crossed with Hoxb7-Cre-GFP mice to induce kidney tubule-specific Vhlh deletion. Kidney tissue was analyzed using histology, immunofluorescence, immunohistochemistry, and flow cytometry. Proliferation, immune infiltration, vascular and lymphatic architecture, as well as functional drainage assays and gene expression profiling, were conducted to characterize phenotypic changes. Vhlh-deficient kidneys exhibited clear-cell morphology, tubular hypertrophy, and a ~ 4-fold increase in Ki67⁺ epithelial proliferation compared to wild-type controls. Immune cell (CD45⁺) infiltration and endothelial cell (CD31⁺) expansion were significantly elevated. Lymphatic architecture was disrupted, with increased numbers of Pdpn⁺ cells, and impaired lymphatic drainage was observed by Evans Blue Dye retention. Pdpn⁺ lymphatic cells expressed high levels of CXCL13, which was validated in both mouse and human ccRCC tissues. BMP10, produced by Vhlh-deficient epithelium, appeared to act through Alk1 signaling to induce CXCL13 expression. CXCL13 in turn promoted proliferation, migration, EMT activation in VHL-deficient epithelial cells via CXCR5. Genetic deletion of CXCL13 or pharmacologic inhibition of Alk1 reduced lymphatic remodeling, inflammation, and epithelial proliferation.
🔗 查看原文

3. ⭐ GSE335365 AKAP6 激酶结合域 (KBD) 肽改善 HFpEF 猪的心脏功能 [scRNA-seq]

✍️ 作者：未知作者
🏷️ 关键词：cardiac、kinase、scRNA
📝 描述：Contributors : Jinliang Li ; Ying Zhu ; Michael KapiloffSeries Type : Expression profiling by high throughput sequencingOrganism : Sus scrofaThe purpose of this RNA-seq analysis is to investigate the effects of KBD expression on gene expression profiles in HFpEF pig hearts.
🔗 查看原文

4. ⭐ GSE304568 靶向巨噬细胞的抗TREM2-CAR T细胞对实体瘤的肿瘤抗原非依赖性靶向治疗

✍️ 作者：未知作者
🏷️ 关键词：tumor、macrophage、antigen
📝 描述：Contributors : Gal Yagel ; Dana Rimini ; Michelle von Locquenghien ; Roberto Avellino ; Pascale Zwicky ; Oren Barboy ; Gaya Granot ; Ken Xie ; Shir Shlomi-Loubaton ; Eyal David ; Kfir Mazuz ; Ido AmitSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusChimeric antigen receptor (CAR) T cell therapy has shown remarkable clinical efficacy in hematological malignancies and, more recently, in autoimmune diseases. However, its application in solid tumors has been limited by tumor antigen heterogeneity, antigen escape, and the immunosuppressive tumor microenvironment (TME), which is particularly shaped by tumor associated macrophages (TAMs). To overcome these barriers, we developed a novel CAR T cell strategy targeting human TREM2⁺ immunosuppressive TAMs. Macrophage-directed anti- TREM2-CAR T cells demonstrated potent anti-TREM2 activity in both in vitro assays and in solid tumor models in vivo. To further enhance intratumoral T cell function, we incorporated a CAR T cell responsive synthetic DNA biosensor comprising NFAT motifs that enable localized secretion of interleukin-12 upon CAR activation. In murine tumor models, this “armored” TREM2-targeted CAR-T cell approach led to robust remodeling of the TME and tumor-draining lymph nodes, characterized by TREM2⁺ TAM depletion, enhanced T and NK cell infiltration and activation, and complete tumor eradication, independent of tumor antigen expression.
🔗 查看原文

5. GSE319614 17种自闭症谱系障碍小鼠模型海马体的批量RNA测序转录组分析

✍️ 作者：未知作者
🏷️ 关键词：RNA-seq、transcriptome
📝 描述：Contributors : Eunjoon Kim ; Junyeop Daniel Roh ; Yukyung Jun ; Mihyun Bae ; Hyojin KangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBulk RNA sequencing was performed on hippocampal tissue from 17 autism spectrum disorder (ASD) mouse models, including both male and female mice. The dataset comprises 269 animals and enables cross-model comparison of transcriptomic alterations associated with ASD-related genetic backgrounds.
🔗 查看原文

6. GSE335057 AKAP6激酶结合域（KBD）肽改善HFpEF猪的心脏功能

✍️ 作者：未知作者
🏷️ 关键词：cardiac、kinase
📝 描述：Contributors : Jinliang Li ; Michael KapiloffSeries Type : Expression profiling by high throughput sequencingOrganism : Sus scrofaThe purpose of this RNA-seq analysis is to investigate the effects of KBD expression on gene expression profiles in HFpEF pig hearts.
🔗 查看原文

7. GSE335831 研究表明，定制化的FcγR阻断疗法可增强免疫检查点疗法的效果并克服耐药性。

✍️ 作者：未知作者
🏷️ 关键词：immune、resistance
📝 描述：Contributors : Niyaz Yoosuf ; Petra Holmkvist ; Linda Mårtensson ; Monika Semmrich ; Ingrid Teige ; Björn FrendeusSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe conducted single-cell RNA sequencing using the 10x Genomics Chromium Next-GEM Flex protocol to investigate the mechanism of action behind the improved therapeutic efficacy of anti-CTLA4 seen when combined with FcgrII blockade. Our goal was to understand the mechanism of action of the combination of anti-CTLA4 and anti-FcgrII in comparison to anti-CTLA4 single treatment. These studies provide new insights and role of FcgrII blockade as therapeutic target in cancer.
🔗 查看原文

8. GSE332607 SETD8介导的YAP K76位点单甲基化促进K48连接的多聚泛素化和降解，从而抑制结直肠癌

✍️ 作者：未知作者
🏷️ 关键词：cancer、methylation
📝 描述：Contributors : Yali Yu ; Haihang Nie ; Hailin Wang ; Jiang Wu ; Fei Xu ; Mei YeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensYes-associated protein (YAP), a key effector of the Hippo pathway, plays a well-established role in colorectal cancer (CRC). However, the functional relevance of site-specific post-translational modifications (PTMs) in YAP, particularly methylation, remains insufficiently explored. SET-domain-containing protein 8 (SETD8), the sole monomethyltransferase for histone 4 lysine 20 (H4K20), is implicated in various cancers, yet its biological function and underlying mechanisms in CRC are elusive. While SETD8 is primarily known for its histone methylation activity, its capacity to modify non-histone proteins, such as YAP, remains largely unexplored. This study aimed to demonstrate that SETD8 exerted a tumor-suppressive effect on CRC by inhibiting YAP protein expression. Mechanistically, SETD8 physically interacts with YAP to catalyze monomethylation at lysine 76 (K76me). This modification enhances the interaction between YAP and the E3 ubiquitin ligase RING finger protein 31 (RNF31), promoting YAP K48-linked polyubiquitination and subsequent proteasomal degradation. Clinically, patients with CRC and high SETD8 expression, including elevated YAP K76me levels, exhibited favorable pathological grading and improved prognosis. Collectively, our findings identify a novel SETD8-YAP K76me regulatory axis that restricts CRC progression, suggesting that targeting this axis may represent a promising therapeutic strategy.
🔗 查看原文

9. GSE339254 APECED 患者和健康对照者外周血细胞的单细胞测序

✍️ 作者：未知作者
🏷️ 关键词：sequencing
📝 描述：Contributors : William Galbavy ; Hyunjin Kim ; Brian Klotz ; Marine Malbec ; Carley Tasker ; Joseph C Devlin ; Benjamin Daniel ; Christina Adler ; Wei Keat Lim ; Asiel A Benitez ; Sokol HaxhinastoSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiens10 APECED and 10 Healthy control patients peripheral blood cells isolated for single cell sequencing. scRNA-seq and BCRseq analysis illustrate changes to the composition of the B cell compartment in APECED compared to Healthy control such as a reduction in Naïve B cells and increases to IgM CD27+ CXCR3+ and Class switched atypical B cell subsets with alternative activation (TBX21+ ZEB2+).
🔗 查看原文

10. GSE330728 17 个 ASD 风险小鼠品系在出生后早期暴露于氟西汀或锂下的单核 RNA 测序图谱（4 重芯片多重测序）

✍️ 作者：未知作者
🏷️ 关键词：RNA-seq
📝 描述：Contributors : Eunjoon Kim ; Junyeop Daniel Roh ; Yukyung Jun ; Mihyun Bae ; Hyojin KangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAutism spectrum disorder (ASD) involves more than 1,000 risk genes, complicating mechanistic subtyping. To resolve the cellular basis of transcriptomic stratification, we generated single-nucleus RNA-seq (snRNA-seq) profiles from the prefrontal cortex of 17 mouse lines carrying ASD-associated synaptic, chromatin-regulatory, and signaling mutations, together with C57BL/6J wild-type controls. Both sexes were profiled under baseline and early-postnatal (P3-P25) fluoxetine or lithium exposure. Four mice were multiplexed per 10x Genomics GEM-X v4 4-plex On-Chip Multiplexing (OCM) library, yielding 53 sequencing libraries (tubes) covering 212 individual mice. After downstream QC, approximately 1,016,895 nuclei from 205 mice were retained at postnatal day 25.
🔗 查看原文

💡 该来源还有 43 条内容，详见文末

🧪 博客更新 (3条)

详细内容（全部3条）

1. 基因影响癌症的发生和发展。

✍️ 作者：未知作者
🏷️ 关键词：cancer
📝 描述：RNA sequencing helped reveal how inherited genetics influence cancer evolution, showing why tumors with similar mutations can grow and respond to treatment differently…
🔗 查看原文

2. 对太空飞行小鼠肺部的RNA测序分析揭示了昼夜节律基因表达的变化

✍️ 作者：未知作者
🏷️ 关键词：RNA-seq
📝 描述：RNA sequencing identified changes in circadian gene expression during spaceflight, providing new insight into how radiation and disrupted biological clocks may increase cancer risk…
🔗 查看原文

3. 一次桑拿浴就能让免疫细胞激增

✍️ 作者：未知作者
🏷️ 关键词：immune
📝 描述：A single 30-minute sauna session may briefly put the immune system on higher alert. Finnish researchers found that sauna heat, paired with a short cold shower, increased every type of white blood cell circulating in the bloodstream, including key infection fighters such as neutrophils and lymphocytes.
🔗 查看原文

📊 关键词统计

关键词	出现次数
transcriptome	23
RNA-seq	11
cancer	4
macrophage	4
tumor	3
immune	2
cardiac	2
kinase	2
scRNA	2
carcinoma	2
single-cell	2
antigen	2
sequencing	1
tumor microenvironment	1
inflammation	1
DNA-seq	1
aging	1
T cell	1
genome	1
resistance	1

📎 更多内容

🧬 数据前沿其他内容 (43条)

📅 报告生成时间：2026-07-29 22:25
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