详细内容（前10条）

1. ⭐ GSE298811 1型糖尿病胰腺淋巴结中免疫失调和干细胞样CD8+ T细胞富集

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、lymph、T cell、regex:lymph(o|atic)?、enrichment
• 📝 描述：Contributors : Leeana Peters ; Howard Seay ; Justin A Smith ; Amanda L Posgai ; Reed L Berkowitz ; Clive H Wasserfall ; Mark A Atkinson ; Rhonda Bacher ; Maigan A Brusko ; Todd M BruskoSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensEffector CD8+ T cells are key drivers of type 1 diabetes (T1D) pathogenesis, yet questions remain regarding the molecular defects leading to altered cytotoxicity, peripheral tissue phenotype, and receptor specificity. We analyzed human pancreatic lymph nodes (pLN) using mass cytometry and single-cell RNA sequencing (scRNAseq) with combined T cell receptor (TCR) profiling. Cytometric analysis revealed enrichment of T stem-cell memory (TSCM)-like cells (CD8+CD45RA+CD27+CD28+CCR7+CXCR3+) in T1D pLNs. scRNAseq indicated an elevated inflammatory cytokine gene signature (IFITM3, LTB) along with regulators of terminal differentiation (BCL6, BCL3), coupled with downregulation of exhaustion-associated genes (DUSP2, NR4A2, TSC22D3) in CD8+ T cells in T1D pLN. Immune Response Enrichment Analysis (IREA) indicated IL-15 signaling as a significant driver of these phenotypes. Integrated TCR and transcriptomic analysis revealed a cluster of diverse naïve-like CD8+ T cell clones in T1D pLN. Comparison of pLN and pancreatic slice isolates supported sharing of effector CD8+ T cells, with enhanced terminal effector signatures within the pancreas relative to paired pLN. Multiplex imaging revealed differential localization of TCF1 and TOX-expressing T cells in the pancreas, with islet-proximal TCF1+TOX+ cells displaying a mixture of activation and exhaustion-associated phenotypes. Thus, we provide multimodal cellular profiles enriched in T1D tissues for consideration in therapeutic targeting.
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2. ⭐ GSE334840 单细胞转录组学揭示了应激与免疫的相互作用，并为疾病风险评估提供了信息。

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、single-cell、transcriptomics
• 📝 描述：Contributors : Sofia Benavides ; Oksana Kosyk ; Haiyang Wang ; Alexander B. Bastian ; Christina Georgiou ; Brian C. Miller ; Natalie Stanley ; Anthony S. ZannasSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensEnvironmental stress contributes to many disease conditions – including cardiovascular disease, mental illness, and autoimmune disorders – through immune dysregulation. Stress operates through the hypothalamic-pituitary-adrenal and sympathoadrenal axes, leading to peripheral secretion of cortisol and norepinephrine, respectively. While stress hormones can profoundly influence immune function, their mechanistic effects at the cell-type level remain poorly understood. To address this knowledge gap, we employed an ex vivo model investigating the transcriptomic responses of primary peripheral blood mononuclear cells and neutrophils to physiological stress levels (i.e., levels reached during in vivo stress) of cortisol and norepinephrine at single-cell resolution. We identified novel stress-hormone-dependent and cell-type-specific transcriptomic changes, including uniquely regulated genes and inflammation-related signatures within distinct innate (e.g., CD163+ monocytes/macrophages, neutrophils) and other immune cell types. The single-cell-derived signatures effectively distinguished individuals with stress-related diseases in two independent human cohorts. Our findings provide cell-type-level insights into how stress hormones modulate immune function relevant to stress-related conditions.
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3. ⭐ GSE314941 研究表明，在唐氏综合征患儿中，黏连蛋白突变会抑制HLA II类基因表达，从而促进免疫逃逸，最终发展为急性白血病。

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、immune、HLA
• 📝 描述：Series Type : Genome binding/occupancy profiling by high throughput sequencing ; Other ; Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
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4. ⭐ GSE336063 单细胞 RNA 测序揭示组氨酰 tRNA 合成酶诱导的肌炎中 CD4+ 组织驻留记忆 T 细胞的克隆扩增 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、RNA-seq、single-cell
• 📝 描述：Contributors : Decheng Li ; Daniel Reay ; Iago Pinal-Fernadez ; Maria Casal-Dominguez ; Andrew Mammen ; Sarah Gaffen ; Timothy Oriss ; Dana AschermanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe precise mechanisms underlying the pathogenesis of idiopathic inflammatory myopathy (IIM) remain undefined. However, there has been increasing recognition that tissue-resident memory cells (TRMs) play an important role in the pathogenesis of systemic autoimmune disease. In IIM, TRM-associated transcriptional signatures have been reported, but on a very limited basis. By using multimodal single-cell RNA sequencing analysis in our established murine model of histidyl-tRNA synthetase (HRS)-induced myositis, we identified a prominent population of CD4+ TRMs in inflamed skeletal muscle. Muscle CD4+ TRMs exhibited high expression of genes encoding Cd69, Cxcr6, Runx3, and Prdm1, alongside low expression of Klf2, Ccr7, Sell, S1pr1, and Tcf7—a profile that is generally consistent with previous reports of TRM gene signature and that we validate through comparison to transcriptomic profiles of human muscle tissue. Detailed pathway analysis in our model indicates that muscle CD4+ TRMs contribute to innate immune regulatory pathways enriched for TNF and IFN-γ signaling. Furthermore, analysis of TCR clonotype distribution and CDR3 sequence similarity revealed pronounced clonal expansion of CD4⁺ TRMs relative to other T-cell subsets—a pattern that remained stable from 2 to 6 weeks post-immunization. Collectively, these results suggest a potential role for CD4+ TRMs in the pathogenesis of autoimmune myositis.
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5. GSE327029 KPNA3 通过上调 MGMT 和激活 STAT3 来维持胶质瘤干细胞，从而驱动胶质母细胞瘤产生替莫唑胺耐药性。

• ✍️ 作者：未知作者
• 🏷️ 关键词：glioma、resistance
• 📝 描述：Contributors : Yanru Gao ; He Yuan ; Houling Zhou ; Chao Gao ; Suling Sun ; Chuanjie He ; Zhiyou FangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensGlioblastoma (GBM) invariably develops resistance to temozolomide (TMZ), the frontline chemotherapeutic agent, leading to treatment failure. The molecular mechanisms underlying this resistance remain incompletely understood. Here, we identify karyopherin subunit alpha 3 (KPNA3) as a novel and critical driver of TMZ resistance. Through integrated bioinformatics analysis of temozolomide-resistant glioma cells (SF126R) and patient databases, we found KPNA3 expression is elevated in TMZ-resistant contexts and correlates with poor prognosis in TMZ-treated patients. Functional studies demonstrated that KPNA3 knockdown in resistant cells impaired malignant behaviors, re-sensitized cells to TMZ-induced apoptosis, and suppressed glioma stem cell (GSC) properties, including sphere formation and expression of stemness factors (OCT4, ALDH1A1). Mechanistically, KPNA3 promotes resistance via a dual pathway: it upregulates the expression of the DNA repair enzyme MGMT and activates the STAT3 signaling pathway. KPNA3 knockdown reduced both MGMT levels and STAT3 phosphorylation (Tyr705). Furthermore, pharmacological inhibition of STAT3 mirrored the effect of KPNA3 knockdown by potently inhibiting GSC generation. Clinically, KPNA3 and p-STAT3 protein levels were positively correlated in high-grade glioma tissues. Collectively, our findings unveil KPNA3 as a central regulator that concurrently enhances DNA repair and sustains the GSC population to foster TMZ resistance, nominating it as a promising therapeutic target for overcoming chemoresistance in GBM.
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6. GSE335842 单细胞 RNA 测序揭示组氨酰 tRNA 合成酶诱导的肌炎中 CD4+ 组织驻留记忆 T 细胞的克隆扩增

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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7. GSE335841 单细胞 RNA 测序揭示了组氨酰 tRNA 合成酶诱导的肌炎中 CD4+ 组织驻留记忆 T 细胞的克隆扩增 [2]

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Contributors : Decheng Li ; Daniel Reay ; Iago Pinal-Fernadez ; Maria Casal-Dominguez ; Andrew Mammen ; Sarah Gaffen ; Timothy Oriss ; Dana AschermanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe precise mechanisms underlying the pathogenesis of idiopathic inflammatory myopathy (IIM) remain undefined. However, there has been increasing recognition that tissue-resident memory cells (TRMs) play an important role in the pathogenesis of systemic autoimmune disease. In IIM, TRM-associated transcriptional signatures have been reported, but on a very limited basis. By using multimodal single-cell RNA sequencing analysis in our established murine model of histidyl-tRNA synthetase (HRS)-induced myositis, we identified a prominent population of CD4+ TRMs in inflamed skeletal muscle. Muscle CD4+ TRMs exhibited high expression of genes encoding Cd69, Cxcr6, Runx3, and Prdm1, alongside low expression of Klf2, Ccr7, Sell, S1pr1, and Tcf7—a profile that is generally consistent with previous reports of TRM gene signature and that we validate through comparison to transcriptomic profiles of human muscle tissue. Detailed pathway analysis in our model indicates that muscle CD4+ TRMs contribute to innate immune regulatory pathways enriched for TNF and IFN-γ signaling. Furthermore, analysis of TCR clonotype distribution and CDR3 sequence similarity revealed pronounced clonal expansion of CD4⁺ TRMs relative to other T-cell subsets—a pattern that remained stable from 2 to 6 weeks post-immunization. Collectively, these results suggest a potential role for CD4+ TRMs in the pathogenesis of autoimmune myositis.
• 🔗 查看原文

8. GSE335819 单细胞 RNA 测序揭示了组氨酰 tRNA 合成酶诱导的肌炎中 CD4+ 组织驻留记忆 T 细胞的克隆扩增 [1]

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Contributors : Decheng Li ; Daniel Reay ; Iago Pinal-Fernadez ; Maria Casal-Dominguez ; Andrew Mammen ; Sarah Gaffen ; Timothy Oriss ; Dana AschermanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe precise mechanisms underlying the pathogenesis of idiopathic inflammatory myopathy (IIM) remain undefined. However, there has been increasing recognition that tissue-resident memory cells (TRMs) play an important role in the pathogenesis of systemic autoimmune disease. In IIM, TRM-associated transcriptional signatures have been reported, but on a very limited basis. By using multimodal single-cell RNA sequencing analysis in our established murine model of histidyl-tRNA synthetase (HRS)-induced myositis, we identified a prominent population of CD4+ TRMs in inflamed skeletal muscle. Muscle CD4+ TRMs exhibited high expression of genes encoding Cd69, Cxcr6, Runx3, and Prdm1, alongside low expression of Klf2, Ccr7, Sell, S1pr1, and Tcf7—a profile that is generally consistent with previous reports of TRM gene signature and that we validate through comparison to transcriptomic profiles of human muscle tissue. Detailed pathway analysis in our model indicates that muscle CD4+ TRMs contribute to innate immune regulatory pathways enriched for TNF and IFN-γ signaling. Furthermore, analysis of TCR clonotype distribution and CDR3 sequence similarity revealed pronounced clonal expansion of CD4⁺ TRMs relative to other T-cell subsets—a pattern that remained stable from 2 to 6 weeks post-immunization. Collectively, these results suggest a potential role for CD4+ TRMs in the pathogenesis of autoimmune myositis.
• 🔗 查看原文

9. GSE327176 单细胞转录组分析揭示肿瘤介导的中性粒细胞重编程及其抑制转移的独特脆弱性

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、single-cell
• 📝 描述：Contributors : Will Putzbach ; Abul Islam ; Maria P Zappia ; Maxim V Frolov ; Nissim HaySeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe performed single cell RNA sequencing (scRNAseq) with the 10x Genomics platform to study how neutrophils are reprogrammed during breast tumorigenesis. Neutrophils acquire a pro-metastatic phenotype induced by signals eminating from the tumor. This comprehensive scRNAseq study assessed neutrophils from various tissues in naive and tumor-bearing MMTV-PyMT mice to uncover how neutrophils are transcriptionally modified. We found that neutrophils are reprogrammed similarly amongst tissues in tumor-bearing mice, and that G-CSF is the main driver of this.
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10. GSE299266 通过对耳蜗中表达pendrin的细胞进行单细胞RNA测序，鉴定出Slc26a4的一种新型亚型

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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详细内容（全部2条）

1. ⭐ scImmuneCo——一个包含细胞类型特异性功能模块的汇编，用于从单细胞RNA测序数据中解码免疫反应。

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、RNA-seq、single-cell
• 📝 描述：Single-cell RNA sequencing powers scImmuneCo, revealing cell-specific gene networks that improve our understanding of immune responses, aging, and disease…
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2. Rectangle——一种基于单细胞RNA测序数据的稳健且可扩展的多尺度反卷积方法

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Rectangle combines bulk and single-cell RNA sequencing to accurately identify cell types and cell states, making large transcriptomic studies more informative and scalable…
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• GSE299257 通过对耳蜗中表达pendrin的细胞进行单细胞RNA测序鉴定Slc26a4的一种新型亚型 - Nanopore
• GSE299255 通过对耳蜗中表达pendrin的细胞进行单细胞RNA测序鉴定Slc26a4的一种新型亚型 - Illumina
• GSE338555 肿瘤间充质干细胞的内皮-间质转化诱导促肿瘤IL-11+癌症相关肌成纤维细胞[TGFB_scRNAseq]
• GSE338554 肿瘤间充质干细胞的内皮-间质转化诱导促肿瘤IL-11+癌症相关肌成纤维细胞[TGFB_bulkRNAseq]
• GSE338553 肿瘤间充质干细胞的内皮-间质转化诱导促肿瘤IL-11+癌症相关肌成纤维细胞[Full_Low_Serum]
• GSE272618 单分子条形码技术用于单细胞基因组学
• GSE339038 RNA-Seq 分析了经 TNF/TNFR 抑制剂组合处理后 MLR 实验中 CD3+ T 细胞的流式分选结果。
• GSE337457 PD-1 调节大脑中 CD4+ T 细胞介导的 CD8+ T 细胞反应，以平衡病毒控制和神经炎症
• GSE280058 利用 CRISPR/Cas9 基因组编辑技术在杜氏肌营养不良症患者来源的 iPSC 中恢复全长肌营养不良蛋白，实现了表型完全恢复，该 iPSC 携带两个外显子的缺失
• GSE339073 小鼠食管EpCAM+上皮细胞的单细胞RNA测序
• GSE338763 小鼠和灵长类动物结直肠癌模型中的炎症性神经病变
• GSE337888 研究表明，青春双歧杆菌的代谢产物在体外和体内均能保护结肠屏障免受炎症引起的破坏。
• GSE330412 人类 GIST-T1 细胞系中雌激素相关受体 (ESRRA) 敲低后的批量 RNA 测序
• GSE329301 METTL1介导的缬氨酸tRNA的m7G修饰驱动胰腺导管腺癌的代谢适应
• GSE269470 对来自 shCtrl 和 shMETTL8 HeLa 细胞的 polyA+ RNA 进行测序以鉴定 m3C 位点
• GSE338421 鉴定南蛇藤活性成分之一桦木酸在胃癌细胞中抗癌作用的分子靶点
• GSE337966 使用基序富集对 CUT&RUN 分析程序进行基准测试
• GSE333188 胱氨酸限制缓解T细胞耗竭并增强CAR-T细胞效力
• GSE333187 胱氨酸限制缓解T细胞耗竭并增强CAR-T细胞效力
• GSE287465 靶向 DDR2 治疗胰腺癌