详细内容（前10条）

1. ⭐ GSE295138 靶向 Regnase-1 可释放 CAR T 细胞抗骨肉瘤活性并创造促炎性肿瘤微环境 [scRNAseq2]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、tumor microenvironment、T cell
• 📝 描述：Contributors : Adeleye Adeshakin ; Hao Shi ; Hongbo Chi ; Stephen GottschalkSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe took advantage of CAR T cells targeting B7-H3 in immune competent osteosarcoma models and evaluated the intrinsic and extrinsic effects of the known negative regulator Regnase-1 (Reg-1).
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2. ⭐ GSE295137 靶向 Regnase-1 可释放 CAR T 细胞抗骨肉瘤活性并创造促炎性肿瘤微环境 [scRNAseq1]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、tumor microenvironment、T cell
• 📝 描述：Contributors : Adeleye Adeshakin ; Hao Shi ; Hongbo Chi ; Stephen GottschalkSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe took advantage of CAR T cells targeting B7-H3 in immune competent osteosarcoma models and evaluated the intrinsic and extrinsic effects of the known negative regulator Regnase-1 (Reg-1).
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3. ⭐ GSE338157 小鼠急性加慢性肝衰竭中肝脏CD45+免疫细胞的单细胞RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、sequencing、single-cell
• 📝 描述：Contributors : Panyu Chen ; Luo ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSingle-cell RNA sequencing was performed on liver CD45+ immune cells isolated from mice subjected to acute-on-chronic liver failure (ACLF). Three experimental groups were included: CCl4-treated control mice, ACLF mice treated with vehicle, and ACLF mice treated with the EP300 inhibitor CCS1477. Male C57BL/6J mice were intraperitoneally injected with carbon tetrachloride (CCl4, 0.2 ml/kg, twice weekly) for 8 weeks. ACLF was induced by administration of a double dose of CCl4 (0.4 ml/kg), followed by intraperitoneal injection of Klebsiella pneumoniae. CCS1477 was administered intraperitoneally 2 hours before bacterial challenge. Liver CD45+ immune cells were isolated 36 hours after K. pneumoniae injection and subjected to BD Rhapsody whole-transcriptome single-cell RNA sequencing.
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4. ⭐ GSE279025 cGAS 缺陷小鼠表现出与染色质组织丧失、LINE1 元件去抑制和炎症诱导相关的早衰 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、inflammation、RNA-seq
• 📝 描述：Contributors : John C Martinez ; Francesco Morandini ; Cheyenne Rechsteiner ; Lucinda Fitzgibbons ; Natasha Sieczkiewicz ; Xiaoyan Liao ; Weijie He ; Sung Jae Bae ; Michael E Meadow ; Eric Hillpot ; Joseph Cutting ; Victoria Paige ; Maxwell Zacher ; Seyed Ali Biashad ; Matthew Simon ; Ines Meula-Zarzuela ; Mario D Cordero ; John Sedivy ; Andrei Seluanov ; Vera GorbunovaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAging-associated inflammation, or ‘inflammaging” is a driver multiple age-associated diseases. Cyclic GMP-AMP Synthase (cGAS) is a cytosolic DNA sensor that functions to activate interferon response upon detecting viral DNA in the cytoplasm. cGAS is also implicated in induction of inflammaging by responding to endogenous signals such as damaged DNA or LINE1 cDNA which forms in aged cells. While cGAS knockout mice are viable their aging has not been examined. Unexpectedly, we found that cGAS knockout mice exhibit accelerated aging phenotype associated with induction of inflammation and DNA damage signaling. Cells from cGAS knockout mice showed increased overall chromatin accessibility, particularly on LINE1 (L1) transposons and inflammation genes. Transcription of L1 elements was also increased associated with high levels of cytoplasmic L1 DNA and expression of ORF1 protein. Stimulated emission depletion microscopy (STED) showed that cGAS forms nuclear condensates that co-localize with heterochromatin. Taken together these results suggest a previously undescribed role for cGAS at maintaining heterochromatin and repressing L1 elements. We propose that cGAS knockout mice lose heterochromatin, leading to derepression of L1 elements and induction of inflammation resulting in premature aging phenotype.
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5. ⭐ GSE279024 cGAS 缺陷小鼠表现出与染色质组织丧失、LINE1 元件去抑制和炎症诱导相关的早衰 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、inflammation、ATAC-seq
• 📝 描述：Contributors : John C Martinez ; Francesco Morandini ; Cheyenne Rechsteiner ; Lucinda Fitzgibbons ; Natasha Sieczkiewicz ; Xiaoyan Liao ; Weijie He ; Sung Jae Bae ; Michael E Meadow ; Eric Hillpot ; Joseph Cutting ; Victoria Paige ; Maxwell Zacher ; Seyed Ali Biashad ; Matthew Simon ; Ines Meula-Zarzuela ; Mario D Cordero ; John Sedivy ; Andrei Seluanov ; Vera GorbunovaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusAging-associated inflammation, or ‘inflammaging” is a driver multiple age-associated diseases. Cyclic GMP-AMP Synthase (cGAS) is a cytosolic DNA sensor that functions to activate interferon response upon detecting viral DNA in the cytoplasm. cGAS is also implicated in induction of inflammaging by responding to endogenous signals such as damaged DNA or LINE1 cDNA which forms in aged cells. While cGAS knockout mice are viable their aging has not been examined. Unexpectedly, we found that cGAS knockout mice exhibit accelerated aging phenotype associated with induction of inflammation and DNA damage signaling. Cells from cGAS knockout mice showed increased overall chromatin accessibility, particularly on LINE1 (L1) transposons and inflammation genes. Transcription of L1 elements was also increased associated with high levels of cytoplasmic L1 DNA and expression of ORF1 protein. Stimulated emission depletion microscopy (STED) showed that cGAS forms nuclear condensates that co-localize with heterochromatin. Taken together these results suggest a previously undescribed role for cGAS at maintaining heterochromatin and repressing L1 elements. We propose that cGAS knockout mice lose heterochromatin, leading to derepression of L1 elements and induction of inflammation resulting in premature aging phenotype.
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6. ⭐ GSE309595 多发性骨髓瘤的纵向分析：肿瘤异质性背景下的治疗反应和免疫微环境动态[scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immune、scRNA
• 📝 描述：Contributors : Aishwarya Chander ; Patrick I McGrath ; Love Tätting ; Yudong D He ; Emma L Kuan ; Marla C Glass ; Samir Rachid Zaim ; Palak C Genge ; Medbh A Dillon ; Cole G Phalen ; Lauren Y Okada ; Zachary J Thomson ; Christian M LaFrance ; Tao Peng ; Peter J Wittig ; Alexander T Heubeck ; Julian Reading ; Charles R Roll ; Veronica Hernandez ; Vaishnavi Parthasarathy ; Tyanna J Stuckey ; Blessing Musgrove ; Elliott Swanson ; Cara Lord ; Morgan D Weiss ; Regina R Mettey ; Kevin J Lee ; John B Johanneson ; Erin K Kawelo ; Jessica Garber ; Upaasana Krishnan ; Xiaoling Song ; Philip D Greenberg ; Lynne A Becker ; Paul Meijer ; Ernest M Coffey ; Thomas F Bumol ; Ananda W Goldrath ; Mackenzie S Kopp ; Evan W Newell ; Peter J Skene ; Damian J Green ; Troy R Torgerson ; Mary Kwok ; Mikael Sigvardsson ; Xiao-jun Li ; Lucas T Graybuck ; Melinda Angus-HillSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMultiple myeloma (MM) is a hematological malignancy characterized by an expansion of malignant plasma cells in the bone marrow. For newly diagnosed MM (NDMM), standard three-drug treatment regimens—such as the combination of bortezomib, lenalidomide, and dexamethasone (VRd)—and the more recent inclusion of a fourth drug, anti-CD38 antibody immunotherapy, have significantly improved patient outcomes by targeting multiple mechanisms of tumorigenesis. This treatment approach typically involves several cycles of VRd induction therapy, followed by high dose melphalan (HDM) and autologous stem cell transplant (ASCT), and maintenance therapy. Treatment responses vary widely, with some patients requiring longer and more intense therapy and others exhibiting more rapid responses and longer relapse-free remission.This cycle of remission, maintenance, and eventual relapse persists despite advancements in treatment modalities, including immunotherapy, leaving MM incurable. This study characterizes the tumor state and immune microenvironment in MM patients across variable graded patient responses. Using a multimodal, longitudinal approach, we investigate immune and microenvironmental cellular changes during treatment in NDMM patients and in a second cohort of RRMM patients. For the NDMM cohort, longitudinal blood and bone marrow aspirate samples were collected at diagnosis, throughout induction therapy, following autologous stem cell transplant (ASCT), and at post-transplant intervals of 60 days, 90 days, and 1 and 2 years. Multi-omic profil…
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7. GSE338608 解冻后骨髓间充质干细胞（BM-MSCs）的单细胞RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、single-cell
• 📝 描述：Contributors : Camila Medrano-Trochez ; Paramita Chatterjee ; Pallab Pradhan ; Hazel Y Stevens ; Molly E Ogle ; Edward A Botchwey ; Joanne Kurtzberg ; Carolyn Yeago ; Greg Gibson ; Krishnendu RoySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHuman mesenchymal stromal cells (MSCs) used in cell therapy are frequently administered directly out of thaw, yet the single-cell transcriptomic landscape of out-of-thaw MSC products is poorly defined. Here, cryopreserved bone marrow-derived MSCs from six healthy donors (seven lots) were characterized immediately after thawing using droplet-based single-cell RNA-sequencing on the Illumina-Bio-Rad ddSEQ platform. After SureCell-based UMI counting and knee filtering, profiles were analyzed with SC3 and Seurat. Donor-level clusters were identified that differ in immune-signaling, cell-surface, cell-cycle and metabolic gene programs, with low within-sample heterogeneity; cell-cycle status emerged as a major axis of inter-donor variation. Only the bone marrow samples are included in this submission.
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8. GSE318683 GAS 缺陷小鼠表现出与染色质组织丧失、LINE1 元件去抑制和炎症诱导相关的早衰 [Cut & Tag]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、inflammation
• 📝 描述：Contributors : John C Martinez ; Francesco Morandini ; Cheyenne Rechsteiner ; Lucinda Fitzgibbons ; Natasha Sieczkiewicz ; Xiaoyan Liao ; Weijie He ; Sung Jae Bae ; Michael E Meadow ; Eric Hillpot ; Joseph Cutting ; Victoria Paige ; Maxwell Zacher ; Seyed Ali Biashad ; Matthew Simon ; Ines Meula-Zarzuela ; Mario D Cordero ; John Sedivy ; Andrei Seluanov ; Vera GorbunovaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusAging-associated inflammation, or ‘inflammaging” is a driver multiple age-associated diseases. Cyclic GMP-AMP Synthase (cGAS) is a cytosolic DNA sensor that functions to activate interferon response upon detecting viral DNA in the cytoplasm. cGAS is also implicated in induction of inflammaging by responding to endogenous signals such as damaged DNA or LINE1 cDNA which forms in aged cells. While cGAS knockout mice are viable their aging has not been examined. Unexpectedly, we found that cGAS knockout mice exhibit accelerated aging phenotype associated with induction of inflammation and DNA damage signaling. Cells from cGAS knockout mice showed increased overall chromatin accessibility, particularly on LINE1 (L1) transposons and inflammation genes. Transcription of L1 elements was also increased associated with high levels of cytoplasmic L1 DNA and expression of ORF1 protein. Stimulated emission depletion microscopy (STED) showed that cGAS forms nuclear condensates that co-localize with heterochromatin. Taken together these results suggest a previously undescribed role for cGAS at maintaining heterochromatin and repressing L1 elements. We propose that cGAS knockout mice lose heterochromatin, leading to derepression of L1 elements and induction of inflammation resulting in premature aging phenotype.
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9. GSE279026 cGAS缺陷小鼠表现出与染色质结构紊乱、LINE1元件去抑制和炎症诱导相关的早衰现象。

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、inflammation
• 📝 描述：Series Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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10. GSE273874 热休克转录因子 HSF1 促进神经元基因组内拓扑异构酶 IIb (TOP2B) 的催化活性

• ✍️ 作者：未知作者
• 🏷️ 关键词：Neuronal、genome
• 📝 描述：Contributors : Lahiri Konada ; Thomas Catley ; Lance Heady ; Morgan Crewe ; Ilse D Ramirez ; Laura Wiggins ; Amir Segev ; Alice L Pyne ; Ram MadabhushiSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusTopoisomerase II (TOP2) poisons, such as etoposide and doxorubicin, are potent antineoplastic drugs that are used to treat a variety of solid tumors and leukemias. Yet these drugs also cause significant secondary malignancies and toxicity to postmitotic cells. Proliferating mammalian cells express two TOP2 isoforms, TOP2A and TOP2B, while postmitotic cells only express TOP2B. Selectively targeting TOP2A, but not TOP2B, could thus prevent secondary toxicity in postmitotic cells, but such isoform-selective targeting strategies remain elusive. Here we report that the heat shock transcription factor, HSF1, facilitates the catalytic engagement of TOP2B on chromatin. Purified recombinant HSF1 stimulates the DNA cleavage and relaxation activity of purified TOP2B. Using atomic force microscopy, we show that HSF1 also enhances the recycling of TOP2B for subsequent rounds of DNA relaxation. TOP2B co-occupies the genome with HSF1 in both postmitotic and dividing cells, and either the knockdown or inhibition of HSF1 reduces the levels of catalytically engaged TOP2B. Intriguingly, HSF1 neither significantly interacts with TOP2A nor enhances its catalytic activity. Furthermore, pharmacological HSF1 inhibitors protect postmitotic cells from the cytotoxicity of TOP2 poisons without compromising their ability to kill cancer cells, revealing a potential strategy for minimizing the side-effects of TOP2 poison-based chemotherapy.
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1. NanoncRNA-seq——一种去除rRNA的全长转录组策略，用于鉴定新的lncRNA异构体

• ✍️ 作者：未知作者
• 🏷️ 关键词：transcriptome
• 📝 描述：A new RNA sequencing workflow using nanopore technology improves full-length lncRNA isoform discovery, revealing many previously undetected transcripts missed by conventional short-read sequencing….
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2. 研究衰老对关键细胞过程的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Combining short- and long-read RNA sequencing reveals how aging alters gene expression, splicing, and transcription, providing new insights into the molecular processes that drive cellular aging…
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3. 食用辣椒可能会增加患上一种致命癌症的风险。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：A major review found that people who consumed the most chili peppers had a substantially higher risk of esophageal cancer, though the evidence was less clear for stomach and colorectal cancers. Researchers emphasize that the findings show an association, not proof of cause and effect, and that more research is needed to determine whether moderate consumption carries similar risks.
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4. 常用的减肥药 Ozempic 和 Wegovy 可能延缓生物衰老

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Researchers found that semaglutide, the active ingredient in Ozempic and Wegovy, slowed biological aging markers in adults with HIV, marking the first clinical evidence that the drug may influence human aging. Although the findings are encouraging, scientists say larger studies are needed before concluding that the medication can help people age more slowly.
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• GSE338597 斑马鱼幼体全器官横断后肠道快速功能性再生受 IL-22 和 IL-26 的拮抗作用调控 [scRNA-seq]
• GSE338307 SRPK2 过表达重塑三阴性乳腺癌细胞的转录组图谱
• GSE316391 原发性和转移性 ER+ 乳腺癌的染色质可及性和基因表达谱分析 [RNA-seq]
• GSE316389 原发性和转移性 ER+ 乳腺癌的染色质可及性和基因表达谱分析 [ATAC-seq]
• GSE309593 多发性骨髓瘤的纵向分析：肿瘤异质性背景下的治疗反应和免疫微环境动态[CITE-Seq]
• GSE273400 G9a/DNMT1 共靶向抑制非小细胞肺癌生长，并通过 SCARA5 和 AOX1 重编程肿瘤细胞以对癌症药物产生反应。
• GSE269963 细菌来源的D-乳酸驱动赖氨酸D-乳酰化，从而调节肝细胞转录
• GSE167159：新冠肺炎康复患者外周血单核细胞单细胞RNA测序与健康对照组的比较
• GSE300282 MeCP2结合和基因组-核纤层重组先于长神经元基因
• GSE333792 纤毛源性胰腺病揭示纤毛病与外分泌胰腺疾病之间的联系 [scRNA]
• GSE299160 研究 NRF2 激活对人食管鳞状细胞癌细胞系（KYSE450 细胞）中 mRNA 表达的细胞行为调节
• GSE299159 研究 NRF2 激活对人食管鳞状细胞癌细胞系（KYSE70 细胞）中 mRNA 表达的细胞行为调节
• GSE338339 共享的 PRAME 表位是 NUT 癌中的 T 细胞靶点
• GSE338159 用 LPS 和 CCS1477 处理的 RAW264.7 巨噬细胞中 EP300 CUT&Tag 全基因组分析
• GSE338158 用 LPS 和 CCS1477 处理的 RAW264.7 巨噬细胞的全基因组 H3K27ac 分析
• GSE337787 氟化物通过组蛋白H3K27乙酰化改变LS8细胞中的基因表达
• GSE333819 人类核糖组揭示DNA嵌入的核糖核苷酸作为DNA超螺旋的表观遗传调节因子
• GSE297046 人类核糖组揭示DNA嵌入的核糖核苷酸作为DNA超螺旋的表观遗传调节因子
• GSE338304 SRPK2 CLIP-seq 揭示乳腺癌细胞中的直接 RNA 靶标
• GSE338289 小鼠耳蜗侧壁衰老相关分子变化的特征分析[GSE197634 的重新分析]
• GSE337980 一种新型自组织胚胎干细胞系统揭示了Wnt信号参数在神经系统前后轴模式形成中的作用
• GSE337380 针对流感抗原的抗体反应
• GSE337273 肥厚型梗阻性心肌病中人类心脏成纤维细胞的转录组特征
• GSE302689 特定阅读障碍的神经细胞模型：迁移和转录组改变的鉴定
• GSE302308 线粒体DNA压缩的诱导限制了先天免疫记忆
• GSE278287：对暴露于左旋多巴/苄丝肼和曲匹地尔的6-OHDA损伤大鼠纹状体进行批量RNA测序
• GSE272205 3D多孔微支架通过促进增殖和自我更新基因网络促进hPSC-巨噬细胞的大规模生产
• GSE237572 黑米提取物处理和对照在不同时间点对秀丽隐杆线虫进行测序
• GSE237549 动物模型小鼠组织样本的 RNA-seq：MMP9 对基因表达的影响。
• GSE167158 通过批量RNA测序对健康人和COVID-19感染康复者的外周血单核细胞进行转录组分析
• GSE335961 MBNL1/2 KD 肌肉中 TA 的批量 RNA 测序
• GSE335902 Pax7-DTA模型中MBL1和2 KD小鼠模型的单核测序
• GSE331509 肢体细胞命运决定是由广泛的协调基因组从核纤层脱离所引导的 [CUT&Tag]
• GSE329903 达拉非尼处理的内皮细胞的 RNA-seq 研究
• GSE319516 肥胖相关的代谢炎症改变了哥廷根小型猪的甲型流感抗病毒反应
• GSE293961 肢体细胞命运决定是由广泛的协调基因组从核纤层脱离所引导的[scDam&T-seq]
• GSE293955 肢体细胞命运决定是由广泛的协调基因组从核纤层脱离所引导的[多组]
• GSE262646 MBL1和2 KD小鼠模型的单核测序