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1. ⭐ GSE326856：IBS-D大鼠肠黏膜单细胞RNA测序图谱，探讨免疫屏障调控在发病机制中的作用

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、RNA-seq、single-cell、regex:intestin(e|al)
• 📝 描述：Contributors : Liyan Ji ; Yongquan Huang ; Jiahe Zhang ; Jiaman Lin ; Zefang Yang ; Xiling Yang ; Fengbin Liu ; Qiuke HouSeries Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusWe have employed a single cell sequencing approach using 10x Genomics scRNAseq to study isolated endothelial cells, immune cells and stromal cells from the intestinal mucosa of the control group and diarrhea-predominant irritable bowel syndrome (IBS-D) rats. This study systematically deciphered the transcriptional landscape of the colonic mucosa in a rat model of IBS-D at single-cell resolution, revealing compositional shifts, transcriptional signatures, and potential regulatory networks among various cellular subpopulations, including epithelial, immune, and stromal cells.
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2. ⭐ GSE289055 P2RY2 由 eATP 驱动，促进癌细胞免疫逃逸和治疗耐药。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune、resistance
• 📝 描述：Contributors : Zhaoqing Hu ; Hitoshi Matsuo ; Chong SunSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn this study, we identify P2RY2 as a pivotal upstream driver of both PGE2 accumulation and B7-H3 upregulation in the TME. This process is fuelled by extracellular ATP (eATP), a cognate ligand for P2RY2 that is commonly and markedly elevated in the TME and rises further under immunotherapies. Genetic or pharmacological disruption of P2RY2 substantially reduces intratumoural PGE2 and B7-H3, increasing T cell infiltration, effector functions, proliferation, and persistence within the TME. Consequently, targeting P2RY2 substantially improves the efficacy of TCR-engineered T cells, CAR-T cells, and immune checkpoint blockade (ICB) in multiple syngeneic and humanized mouse tumour models, and potentiates patient-derived tumour-infiltrating lymphocytes (TILs) against paired primary tumour cells. By establishing P2RY2 as the crucial link between eATP and tumour-induced immunosuppression, our study reveals how a receptor typically associated with innate “danger” signalling can be hijacked to undermine adaptive immunity, thereby providing a promising therapeutic avenue to overcome a common immune resistance mechanism in cancer and advance T cell–based cancer therapies.
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3. ⭐ GSE337829 RNA 和 DNA 相互作用组的单细胞整合分析揭示了癌症中可靶向的染色质结构 [ChIP-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、ChIP-seq、single-cell
• 📝 描述：Contributor : Evgeny DeforzhSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensThe human genome is pervasively transcribed into protein-coding and regulatory non-coding RNAs whose functions are coordinated within higher-order nuclear architectures. However, direct mapping of RNA–RNA and DNA–DNA interaction networks in complex human tissues at single-cell resolution has remained a major challenge. Here, we present SCIENCE-seq, a multimodal single-cell interactomics platform enabling simultaneous detection of RNA–RNA interactions and chromatin DNA–DNA contacts within individual cells. Applied to primary glioma specimens, SCIENCE-seq reveals cancer-specific interactions organized by lncRNAs and centered on key oncogenic drivers, including EGFR, hTERT, SOX2, CDK6, CDC42, CD47, and HOX loci. These datasets uncover previously unrecognized molecular relationships between premature lncRNAs and pre-mRNAs that regulate transcription, alternative splicing, and polyadenylation. Notably, we identify a glioma-specific trans-chromosomal HOX hub driven by five interacting lncRNAs. Targeting these RNA and DNA interactions with steric antisense oligonucleotides (ASOs) or CRISPRi enables selective inhibition of oncogenic programs in malignant cells while sparing normal tissue, establishing chromatin interactomes as actionable therapeutic targets.
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4. GSE326006 在胶质瘤模型中，对流增强地塞米松递送可抑制髓系炎症，同时避免全身毒性

• ✍️ 作者：未知作者
• 🏷️ 关键词：inflammation、glioma
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusDexamethasone is widely used to control cerebral edema and inflammation in glioblastoma, but its benefits are limited by systemic toxicities and adverse prognostic associations. We evaluated local administration of dexamethasone via convection-enhanced delivery (CED) to maximize intratumoral anti-inflammatory effects by increasing local corticosteroid exposure while minimizing systemic exposure. In two glioma mouse models, continuous intraparenchymal infusion of dexamethasone was well tolerated with no adverse effects. Pharmacokinetic analyses supported preferential intratumoral distribution and reduced systemic exposure with CED compared with systemic dosing. Single-nucleus RNA sequencing (snRNA-seq) and immunohistochemistry showed attenuation of glioma-associated inflammation with downregulation of reactive microglial/macrophage programs and reduced tumor-infiltrating myeloid cells with a morphology consistent with a less activated state. Experiments in human induced pluripotent stem cell (iPSC)–derived microglia confirmed that dexamethasone directly suppresses inflammatory gene expression, indicating a conserved mechanism across species. This inflammatory suppression was recapitulated in both immortalized microglial (HMC3) and macrophage (THP1) cell lines. These findings suggest that localized dexamethasone delivered by CED reprograms the glioma immune microenvironment and achieves control of inflammation without the systemic adverse effects associated with standard systemic dexamethasone therapy. This clinically translatable strategy may improve symptom management and provide a platform for integrating local immunomodulation with future glioblastoma therapies.
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5. GSE313660：卵泡液单细胞分析揭示接受卵巢刺激的多囊卵巢综合征患者排卵免疫功能失调

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、single-cell
• 📝 描述：Contributors : Andrea Wegrzynowicz ; Soma Banerjee ; Emily Grimes ; Riley Huddleston ; Fernanda Leyva Jaimes ; Laura G Cooney ; Aleksandar K StanicSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensPolycystic ovary syndrome is the most common endocrine condition in women and anovulatory cause of female infertility. While a pro-inflammatory cytokines and leukocyte bias in systemic circulation is well-documented in PCOS, it is not known how this inflammation extends to or affects the ovary. Additionally, the relationship between ovulation and inflammation in PCOS is not well-defined. We hypothesize that the ovarian follicular immune environment in PCOS is uniquely dysregulated, and that resolving anovulation through ovulation induction is not sufficient to alleviate this dysregulation. Using single-cell RNA and surface protein analysis of peripheral blood and follicular fluid from patients undergoing in vitro fertilization, we discovered that both control and PCOS follicles were immunologically distinct from circulation. At a systemic level, we find that ovulation induction in PCOS does not alleviate systemic inflammation. In contrast, while healthy control ovaries experienced acute immune-directed ovulatory signaling, PCOS ovarian follicles were deficient in key pro-ovulatory cell to cell communication, and displayed instead a chronic low-grade inflammatory state with fibrotic features. Taken together, a picture emerges where acute ovulation demonstrates a well-ordered series of follicle-specific immune information flows, which are disrupted and replaced by low grade chronic inflammation in the PCOS follicle.
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6. GSE306798 单细胞转录组基因调控网络分析揭示了Nr4a1基因敲除小鼠结肠干细胞和免疫细胞稳态的改变

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、single-cell
• 📝 描述：Contributors : Selim Romero ; Shreyan Gupta ; Michael L Salinas ; Destiny A Mullens ; Jennifer S Goldsby ; Yang Yi Fan ; Laurie A Davidson ; Jaileen Rivera Rodriguez ; Gus A Wright ; Steven H Safe ; James J Cai ; Robert S ChapkinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusRecent findings suggest that the orphan nuclear receptor 4A (NR4A) can regulate cellular energetics and epithelial defenses, contributing to a resilient disease resistant phenotype. However, its role in regulating intestinal crypt homeostasis remains unclear. To assess the effects of NR4A on intestinal epithelial, stromal and immune cell heterogeneity and functional phenotypes, we utilized single-cell transcriptomics and advanced analytic strategies to generate a high-quality atlas for the colonic epithelium from wild-type and whole body Nr4a1 knockout mice. Here we observed a widespread effect of Nr4a1 deletion on intercellular communication involving intestinal stem cells, macrophages, T and B cells, and fibroblasts, indicating a complex and pervasive remodeling of the colonic epithelial and immune microenvironments and their regulatory networks. This alteration in cell-cell crosstalk was associated with an elevated single-cell entropy, indicative of higher differentiation potential and stem-like properties in epithelial, stromal and immune cell types. Our work provides new evidence of the molecular function of Nr4a1 as a critical regulatory factor in stem cell and immune homeostasis in the colon.
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7. GSE300933 Ptpn2 通过整合 B 细胞受体和 IFN-γ 信号来限制浆细胞的命运和抗病毒免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity、B cell
• 📝 描述：Contributors : Hincapie Ana Maria ; Tremblay Michel L.Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusB cell activation and subsequent plasma cell differentiation are two crucial processes in the establishment of long-term immunity. Understanding the signaling pathways that regulate B cell function and terminal differentiation is crucial to human health. Ptpn2 is a protein tyrosine phosphatase whose role in limiting immune cell activation is well chatacterized. However, it is not known whether Ptpn2 plays a cell-intrinsic role in regulating B cell development and activation. Using a Ptpn2 B-cell-specific knockout mouse model we have discovered a novel role for Ptpn2 in negatively regulating both BCR and IFN-gamma signaling in B cells. As a result, the absence of Ptpn2 leads to the enhanced terminal differentiation of B cells into plasma cells. Consequently, mice lacking Ptpn2 in B cells present improved antibody responses against viral infections, such as Influenza A virus. Overall, our results reveal a novel role for Ptpn2 in B cell activation, highlighting its potential as a therapeutic target to optimize vaccine responses and enhance immunity against infectious diseases.
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8. GSE264164 RNA-seq 和 ATAC-seq 分析了滤泡 B 细胞和生发中心 B 细胞中 Brwd1 条件性缺失的情况

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、ATAC-seq
• 📝 描述：Series Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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9. GSE264163 RNA-seq 和 ATAC-seq 分析了滤泡 B 细胞和生发中心 B 细胞中 Brwd1 条件性缺失的情况

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、ATAC-seq
• 📝 描述：Contributors : Nathaniel E Wright ; Domenick E Kennedy ; Junting Ai ; Margaret L Veselits ; Mary Attaway ; Young M Yoon ; Madeleine S Durkee ; Jacob Veselits ; Mark Maienschein-Cline ; Malay Mandal ; Marcus R ClarkSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusFollicular B cells and germinal center B cell subsets were sorted from Cd23-Cre Brwd1fl/fl and Aicda-Cre Brwd1fl/fl mice, respectively.
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10. GSE264161 RNA-seq 和 ATAC-seq 分析了滤泡 B 细胞和生发中心 B 细胞中 Brwd1 条件性缺失的情况

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、ATAC-seq
• 📝 描述：Contributors : Nathaniel E Wright ; Domenick E Kennedy ; Junting Ai ; Margaret L Veselits ; Mary Attaway ; Young M Yoon ; Madeleine S Durkee ; Jacob Veselits ; Mark Maienschein-Cline ; Malay Mandal ; Marcus R ClarkSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusFollicular B cells and germinal center B cell subsets were sorted from Cd23-Cre Brwd1fl/fl and Aicda-Cre Brwd1fl/fl mice, respectively.
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1. 美国国立卫生研究院的“我们所有人”研究项目目前是世界上最大的综合基因组学和健康数据库。

• ✍️ 作者：未知作者
• 🏷️ 关键词：genomics
• 📝 描述：RNA sequencing joins genomic, proteomic, and clinical data in the expanded NIH All of Us database, creating an unprecedented resource for precision medicine research…
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2. 肌酸不仅能促进肌肉生长，它还可能有助于对抗癌症。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have discovered that creatine may strengthen one of the immune system’s most important cancer-fighting pathways by energizing dendritic cells that activate killer T cells. The promising results could eventually help make immunotherapy more effective, but they have not yet been tested in human patients.
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• GSE305945 Mov10 通过调节 TNFα–NF-κB 通路抑制过度炎症
• GSE337822转录组特征揭示了麻醉和睡眠激活的重叠共享和独特但状态特异性的视前神经元群
• GSE337601 XCR1+ 和 CD11b+ 迁移树突状细胞协同作用，促进肿瘤内 CD8+ T 细胞的交叉启动，使其具有组织驻留记忆表型 [RNA-Seq]
• GSE312032 通过将优化的 CAR 胞内结构域与新型 iNKT 衔接器相结合来增强 iNKT 细胞免疫治疗平台
• GSE311240 人类神经元分化的长读长蛋白质基因组图谱揭示了同工型多样性，为神经发育风险机制提供了信息。
• GSE310273 CRISPR扰动CD8⁺ T细胞后年轻肿瘤与老年肿瘤的ATAC-seq
• GSE310131 人类神经元分化长读长蛋白质组图谱揭示了同工型多样性，为神经发育风险机制提供了信息
• GSE337872 卡波西肉瘤单细胞转录组分析
• GSE337492 CD300A 通过 JAK2/STAT3 信号通路调控 M2 巨噬细胞极化，从而驱动胶质母细胞瘤进展。
• GSE335783 METTL3 作为雄激素受体共调节因子，在促进前列腺癌增殖方面发挥非酶促作用。
• GSE302168 GLI2介导三阴性乳腺癌对紫杉醇的反应
• 来自MDA-MB-231 shYB-1和sh对照的GSE117856 RNA测序