详细内容（前10条）

1. ⭐ GSE334014 哮喘患者和对照组的气道微生物组多样性、粘膜内细菌和空间免疫。

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity、bacteria、regex:bacter(ia|ial|ium)、spatial
• 📝 描述：Contributors : Miriam Moffatt ; Tamiko Nishimura ; William CooksonSeries Type : OtherOrganism : Homo sapiensGlobal human gene expression along with a custom panel targeting 29 common respiratory microbes were quantified in epithelial and stromal compartments of 44 asthmatic adults and controls using GeoMx digital spatial profiling (DSP) Whole Transcriptome Assay (WTA) with NGS read-out.
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2. ⭐ GSE322490 MT2A敲低卵巢癌细胞的转录组测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、sequencing、transcriptome
• 📝 描述：Contributor : Joe R DelaneySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensOvarian cancer cells were knocked down with shRNA targeting MT2A or a scrambled control vector. Whole transcriptome sequencing was performed on actively proliferating cells.
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3. ⭐ GSE337417 对用玉竹提取物治疗的对乙酰氨基酚诱导急性肝损伤小鼠的肠道菌群进行16S rRNA基因扩增子测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Huanghui Qin ; Runxiao Chen ; Ying Shi ; Caibin Li ; Yiming Bin ; Zhaoming Zeng ; Bin Deng ; Lanyu LiSeries Type : OtherOrganism : Mus musculus16S rRNA V3-V4 amplicon sequencing was performed on fecal samples from 12 male C57BL/6J mice across three groups (4 replicates each): Control (distilled water, 3 days), Model (distilled water, 3 days + APAP 300 mg/kg i.p.), and PoH (PO extract 3 g/kg p.o. daily for 3 days + APAP 300 mg/kg i.p.). Sequencing was performed on an Illumina NovaSeq 6000 platform using paired-end 250bp reads.
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4. ⭐ GSE337336 基因组和空间转录组学揭示了蕈样肉芽肿早期进展中成纤维细胞相关的微环境

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Myoung E Choi ; Ji H Choi ; Hwa-Jeong Shin ; Chong H Won ; Sung E Chang ; Mi W Lee ; Woo J LeeSeries Type : OtherOrganism : Homo sapiensMycosis fungoides (MF) is the most common cutaneous T-cell lymphoma. To characterize the tumor microenvironment at single-cell spatial resolution, we applied NanoString CosMx Spatial Molecular Imaging (SMI) with a 1000-plex panel to formalin-fixed paraffin-embedded (FFPE) skin biopsies from MF patients. This dataset includes patch-stage and plaque-stage MF lesions from 16 patients, comprising 65,717 cells profiled across multiple fields of view per patient.
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5. ⭐ 果蝇 Psc Su(Z)2 -/- 肠道干细胞的 GSE299390 RNA-seq 和 ATAC-seq

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、ATAC-seq、regex:intestin(e|al)
• 📝 描述：Contributors : Ruxue Wei ; Rongwen XiSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Drosophila melanogasterPolycomb group (PcG) genes function as epigenetic silencers that maintain the repressive states of target genes essential for normal development. To elucidate the role of PRC1 components in the Drosophila midgut, we performed both RNA-seq and ATAC-seq analyses on intestinal stem cells (ISCs) deficient for PRC1 and Su(z)2. For RNA-seq controls, besides the esgts>GFP, we included ISCs harboring mutations in the Notch signaling pathway (N55e11) and the JAK-STAT pathway (dome468) as additional controls. What’s more, ATAC-seq was also performed on pc-RNAi and E(z)-RNAi ISCs which represent other components of PRC1 and PRC2, respectively.
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6. ⭐ GSE275721 谱系主调控因子和癌症选择性伴侣转录因子重塑三维基因组拓扑结构，实现肿瘤特异性基因调控

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、genome
• 📝 描述：Series Type : Genome binding/occupancy profiling by high throughput sequencing ; Other ; Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
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7. GSE327707 人类外周血单核细胞群在不同体外免疫刺激下的分选批量 RNA-seq 分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、RNA-seq
• 📝 描述：Contributors : Oliver Wood ; Adam T Braithwaite ; Matthew A Jackson-WoodSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHuman peripheral blood mononuclear cells (PBMCs) from healthy, de-identified donors were stimulated in vitro with a subset of immune stimuli and profiled by bulk RNA sequencing following fluorescence-activated cell sorting (FACS). Following a 48-hour assay, major immune cell populations were isolated, including monocytes, CD4+ T cells, CD8+ T cells, and B cells, and subjected to RNA-seq. This dataset captures cell type-specific transcriptional responses to B cell stimulator cells (BCS), CytoStim™, lipopolysaccharide (LPS), and IFN-α, alongside unstimulated baseline controls.
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8. GSE294116 利用高精度全长RNA测序推进斑马鱼功能转录组学研究

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、transcriptomics
• 📝 描述：Contributors : Monika Kwiatkowska ; Marta Blangiewicz ; Tomasz Mądry ; Silvia Carbonell-Sala ; Guigo Roderic ; Barbara Uszczynska-RatajczakSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioZebrafish is an invaluable model organism due to its anatomical and genetic similarities to humans, including shared major organs and tissues. These similarities make findings from zebrafish studies more applicable to human biology than those from in vitro or non-vertebrate models. Despite having one of the best annotated genomes among model organisms, zebrafish genome annotations have seen limited updates in recent years, lagging significantly behind human annotations. This outdated information contributes to inaccuracies in RNA sequencing analysis, gene quantification, and other downstream analyses, reducing zebrafish’s effectiveness as a model. To address these issues, we implemented CapTrap-seq, a platform-agnostic approach to sequence full-length zebrafish transcriptomes across key developmental stages and adult tissues. Our findings indicate that CapTrap-seq delivers manual-quality genome annotations at high-throughput, helping to complete and expand zebrafish gene information and link it to broad biological contexts.
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9. GSE337111 通过对血管紧张素 II (Ang II) 诱导的心脏肥大重塑小鼠模型进行 RNA 测序分析，鉴定了心室组织中的基因表达变化。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cardiac、sequencing
• 📝 描述：Contributors : Shu-Jing Liu ; Wei-Jia Yu ; Qing-Song Li ; Shi-Nong Yu ; Zhi-Chao Dong ; Hui-Hua Li ; Qiu-Yue LinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusForsythoside I (FI), a representative caffeoyl phenylethanoid glycoside isolated from Forsythia suspensa, has been previously validated to exert anti-inflammatory and anticancer effects. Nevertheless, research regarding its potential role in cardiovascular diseases, particularly pathological cardiac hypertrophy, and the underlying molecular mechanisms ‌remains‌ largely unknown.
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10. GSE330002 高通量筛选原发性胶质母细胞瘤相关巨噬细胞，发现 HDAC 抑制剂可增强吞噬作用，是免疫疗法的有效伙伴 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:immuno(logy|therapy|suppression)、RNA-seq
• 📝 描述：Contributors : Mona Khalaj ; Irving L WeissmanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis study presents the first high-throughput chemical screen on primary tumor-associated macrophages (TAMs) freshly isolated from surgical glioblastoma (GBM) patient samples, testing 1,365 compounds to identify drugs that enhance TAM phagocytosis. HDAC inhibitors, particularly Pracinostat and Resminostat, emerged as potent enhancers of phagocytosis, demonstrating synergy with CD47 blockade across multiple primary human TAM-GBM combinations. In a xenograft GBM model, Pracinostat suppressed tumor growth and extended survival with additive benefit when combined with CD47 antibodies. RNA-seq and H3K27Ac CUT&Tag profiling of Pracinostat-treated TAMs in vivo revealed a two-tier mechanism: transcriptional reprogramming toward a pro-inflammatory state via NF-kB activation, and epigenetic priming of FcgR-mediated phagocytic machinery, providing a mechanistic basis for the observed synergy with CD47 blockade.
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详细内容（全部3条）

1. 人工智能模型提高了对多种癌症免疫疗法反应的预测能力

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:immuno(logy|therapy|suppression)
• 📝 描述：RNA sequencing combined with artificial intelligence improves prediction of immunotherapy response across multiple cancers while revealing biological mechanisms linked to treatment success and resistance… The post AI model improves prediction of immunotherapy response across cancers appeared first on RNA-Seq Blog.
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2. 利用细胞云模型重新思考免疫细胞

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune
• 📝 描述：RNA sequencing reveals that immune cells exist along continuous landscapes of changing identities, providing a new framework for understanding cell behavior and improving future immunotherapies… The post Rethinking immune cells with the cell cloud model appeared first on RNA-Seq Blog.
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3. 科学家解开了困扰人们30年的黑麦花粉之谜，这可能彻底改变癌症研究。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have finally solved a nearly 30-year-old mystery surrounding two unusual molecules found in rye pollen that once showed an intriguing ability to help animals fight tumors. By determining their exact 3D structures, researchers have unlocked the blueprint needed to investigate how these natural compounds interact with the immune system and which parts may be responsible for their cancer-fighting effects.
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• GSE309608 空间分辨的前庭神经节神经元细胞类型的形态和功能多样性 [scRNA-seq]
• GSE307301 mTORC1 激活诱导肾细胞癌 (RCC) 中 B7-H3 依赖性代谢重编程
• GSE337655 单个转录因子亚型导致连接组和转录组身份的转变
• GSE327907 人类外周血单核细胞在多种体外免疫刺激下的整合单细胞转录组分析
• GSE310399 有效新生嵌合抗原受体的序列和结构决定因素
• GSE276559 α-突触核蛋白阻断帕金森病中的内质网蛋白转位 [scRNA-seq]
• GSE276377 α-突触核蛋白阻断帕金森病中的内质网蛋白转位[批量RNA测序]
• GSE326216 ADAR1通过cIAP2/NF-κB通路和C16orf46 RNA编辑介导的细胞焦亡，在食管鳞状细胞癌（ESCC）中调控放射敏感性的双重机制
• GSE325987 强效的特异性新生抗体可补充广谱反应性印迹抗体对SARS-CoV-2变异株的免疫反应
• GSE298301 套索状siRNA通过PTGS通路介导内源基因沉默和翻译抑制
• GSE284769 牛人工授精和体外受精妊娠子宫细胞外囊泡转录组分析
• GSE284626 套索状siRNA通过PTGS通路介导内源基因沉默和翻译抑制
• GSE337786 整合多组学揭示皮肤癌侵袭性微环境的细胞和分子相互作用 II
• GSE337460 子宫内衣原体血清型 - scRNA-seq、CITE-seq 和 TCRab
• GSE337438 靶向肿瘤治疗的mRNA药物的肿瘤选择性蛋白翻译
• GSE337426 USP22 是 KMT2Ar 急性白血病中调节 MEIS1 蛋白丰度和基因转录的新型脆弱性。
• GSE337357 库欣病患者垂体腺瘤的单细胞转录组分析
• GSE334628 RNA-seq 分析揭示了顺铂和二氧化钛纳米颗粒联合作用诱导的果蝇转录紊乱
• GSE332930 腱细胞分化后干细胞的单细胞RNA测序分析
• GSE301989 IL1R2 过表达与 S100P 和 LEF1 协同作用介导早产儿免疫抑制
• GSE301872 治疗持续性白血病对通过靶向 KHSRP 恢复 SLC23A1 具有选择性敏感性（来自不同疾病状态的 PDX 样本）
• GSE301866 治疗持续性白血病对通过靶向 KHSRP 恢复 SLC23A1 具有选择性敏感性 [eCLIPseq]
• GSE301790 RNA测序分析PFAS对肝脏类器官的影响
• GSE301622 RNA测序研究CXCR3受体调控对活化T细胞功能的影响
• GSE300492 早期生命中 NuA4 复合物活性对晚年蛋白质稳态和寿命的发育编程 [RNA-Seq]
• GSE330452 酰基辅酶A合成酶3 (ACSF3) 的昼夜节律调控线粒体赖氨酸丙二酰化和肝脏每日代谢
• GSE330003 高通量筛选原发性胶质母细胞瘤相关巨噬细胞，发现 HDAC 抑制剂可增强吞噬作用，是免疫疗法的有效伙伴 [CUT&Tag]
• GSE312260 伊立替康敏感和伊立替康耐药结直肠癌类器官单细胞水平基因表达谱
• GSE309565 空间分辨的前庭神经节神经元细胞类型的形态和功能多样性