详细内容（前10条）

1. ⭐ GSE271601 面肩肱型肌营养不良症的新视角：肠道微生物群与D4Z4甲基化的关联

• ✍️ 作者：未知作者
• 🏷️ 关键词：methylation、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Ceren Hangul ; Ozlem Koyuncu Ozyurt ; Simone Baldı ; Dilek Colak ; Oznur Tokta ; Toygar Pekcan ; Sara Bertorello ; Marco Pallecchi ; Filiz Koc ; Cumali Dolakay ; Hilmi Uysal ; Kemal Hakan Gulkesen ; Filiz Ozcan ; Gianluca Bartolucci ; Sibel Berker Karauzum ; Amedeo AmedeıSeries Type : OtherOrganism : Homo sapiensThe objective was investigated the compositional and functional features of the gut microbiota (GM) and their relation to D4Z4 methylation in Facioscapulohumeral muscular dystrophy (FSHD) patients.
• 🔗 查看原文

2. GSE337503 RNA-seq 分析 CSNK2B 和 P300 在 NCI-H69 小细胞肺癌细胞中的敲低情况

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、RNA-seq
• 📝 描述：Contributors : Chen Yuli ; Chen Qinnan ; Guo Jiahao ; Li Ziwei ; Yu Shaokun ; Huang Peng ; Sun MingSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis submission contains bulk RNA-seq data from NCI-H69 small cell lung cancer cells transfected with negative control siRNA, CSNK2B-targeting siRNA, or P300-targeting siRNA. Total RNA was collected 48 hours after transfection. The dataset was generated to investigate transcriptional programs regulated by the CSNK2B/RBBP4/P300 complex in small cell lung cancer, particularly genes and pathways related to DNA damage repair and NF-kappa B signaling.
• 🔗 查看原文

3. GSE337023 全基因组倍增的三阴性乳腺癌细胞群的基因组和转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、genome
• 📝 描述：Contributors : Howland Kennedy ; Brock AmySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate whether the mechanism of whole-genome doubling (WGD) results in distinct genomic and transcriptomic phenotypes, we compared three routes to WGD against their respective parental aneuploid controls across two triple-negative breast cancer (TNBC) cell lines (HCC1806 and MDA-MB-231). WGD mechanisms included naturally occurring cell-cell fusion and two drug-induced cell-cycle error mechanisms: mitotic slippage (MS) and cytokinesis failure (CF). All populations were profiled in biological triplicate by TagSeq (3’ tag-based RNA-seq) for transcriptomic analysis and low-pass whole-genome sequencing (WGS) for copy-number profiling.
• 🔗 查看原文

4. GSE303772 发育过程中不同的肺泡巨噬细胞介导香烟烟雾引起的炎症和损伤 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：inflammation、RNA-seq
• 📝 描述：Contributors : Marvin Hering ; Nina Frey ; Dilay Cansever ; Atul Sethi ; Pablo Hernández-Malmierca ; Mika Manser ; Jordan Poirot ; Said Aktas ; Desiree von Tell ; Anna Mechling ; Kara Lassen ; Nikica Miše-Racek ; Thomas Marichal ; Emma DoranSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe employed bulk RNA sequencing to investigate how varying durations of cigarette smoke (CS) exposure affect alveolar macrophages (AM) of different ontogenies in mice. Chronic obstructive pulmonary disease (COPD) is often associated with CS and the third leading cause of death. While airway-recruited macrophages contribute to COPD pathogenesis, the role of embryonic AM (Em-AM) remains unclear. Using a CS-induced COPD mouse model, we found that CS-triggered lung inflammation and emphysema-like tissue damage correlated with substantial changes in the lung myeloid compartment. Using fate-mapping, we showed that, while most CS-induced AM were recruited bone marrow monocyte-derived AM (BM-AM), Em-AM persisted and underwent similar inflammation- and emphysema-associated adaptations as BM-AM upon CS. Therefore, our data support that the CS-exposed niche, rather than ontogeny, is the main determinant of AM fate in COPD, and that CS-induced AM changes may be potential therapeutic targets to mitigate AM dysfunction in COPD.
• 🔗 查看原文

5. GSE301774 工程化可逆植物激素诱导型“开启开关”可提高CAR-T细胞免疫疗法的安全性和有效性

• ✍️ 作者：未知作者
• 🏷️ 关键词：T cell、regex:immuno(logy|therapy|suppression)
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe ability to externally regulate chimeric antigen receptor (CAR) activity via small molecules offers a promising strategy to enhance the safety and precision of adoptive T cell therapies. We present a novel inducible ON-switch CAR design that leverages plant hormone signaling components to achieve controllable T cell activation. Specifically, we engineered a receptor system that integrated the plant auxin receptor IAA7 and its co-receptor AFB1, enabling ligand-dependent interactions in response to the plant hormone auxin. Our results demonstrated that this auxin-inducible CAR (auxCAR) mediated rapid, reversible, and dose-dependent T cell activation, leading to potent cytotoxicity against Raji B-cell lymphoma in vitro and in vivo. Notably, auxCAR-T cells maintained a favorable memory T cell phenotype and exhibited reduced exhaustion markers compared with conventional CAR-T cells, translating into improved therapeutic efficacy. This plant hormone-based control system offers a versatile and orthogonal approach for precise modulation of CAR activity, with significant implications for the development of safer, more adaptable immunotherapies.
• 🔗 查看原文

6. GSE326370 雄性宝山猪七个生殖轴组织的单细胞短读长和长读长转录组图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell
• 📝 描述：Contributors : Jinglong Huo ; Hai LiSeries Type : Expression profiling by high throughput sequencingOrganism : Sus scrofaSingle-cell transcriptomic data were generated from seven reproductive-axis tissues of an 18-month-old male Baoshan pig, including hypothalamus, pituitary, prostate, bulbourethral gland, seminal vesicle, epididymis, and testis. To characterize both gene-expression profiles and transcript isoform diversity across these tissues, single-cell short-read libraries were sequenced on an Illumina platform and single-cell long-read libraries were sequenced on an Oxford Nanopore platform. Short-read data were processed with Cell Ranger, whereas long-read data were processed with FLAMES and further assessed for isoform structure. The dataset provides cell-level gene expression matrices, transcript/isoform-level quantification, isoform annotation files, and accompanying cell metadata. These data may facilitate studies of cellular heterogeneity and transcript isoform usage across the porcine reproductive axis.
• 🔗 查看原文

7. GSE337368 研究 IFN-γ 对贴壁和脱落卵巢癌细胞的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Contributors : Emilija Aleksandrovic ; Siyuan ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe interplay between intrinsic and niche-driven mechanisms that enables DTCs to survive and home to distant organs remains incompletely understood. Single-cell RNA sequencing (scRNA-seq) coupled with velocity analyses in ascites and metastasis-bearing omenta uncovered an emergent and distinct interferon-gamma centric transcriptional trajectory, enriched among early seeding clones. Knockout of IFN-gamma receptor 1 (Ifngr1) in tumor cells significantly reduced metastatic burden and extended survival, underscoring the importance of tumor cell intrinsic IFN-gamma signaling in ovarian cancer metastasis. We identified that the tumor intrinsic IFN-gamma response and ascites-derived tumor-associated macrophages (TAMs) protect cancer cells from anoikis-mediated death. To investigate the mechanism underlying increased cancer cell survival under IFN-gamma treatment and ULA conditions, we performed bulk RNA-sequencing. We discovered increased expression of the anti-apoptotic gene Parp14 in cancer cells treated with IFN-gamma, specifically under ultra low attachment conditions.
• 🔗 查看原文

8. GSE309120 研究早期和晚期播散性肿瘤细胞在肺转移进展中的作用

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor
• 📝 描述：Contributors : Emilja Aleksandrovic ; Siyuan ZhangSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusRecent pioneering work in the field has not only described the presence of early neoplasm-derived cancer cells in the metastatic niche but has elucidated several underlying mechanisms responsible for their seeding. Yet, it remains unclear how early cancer cells interact with their new cellular environment once disseminated. In the present work, we aimed to address the knowledge gap related to how the immune microenvironment responds to and influences the fate of early compared to later arriving disseminated tumor cell (DTC) seeds. To distinguish early vs. late seeding cancer cells, we first engineered barcoded cancer cell lines and then conducted multiple, staggered injections in a triple negative breast cancer (TNBC) lung metastasis context. Our preliminary data showed that early timepoint injected cancer cells preferentially proliferate despite not harboring genetic and transcriptomic advantages compared to later arriving cancer cells. Furthermore, single cell RNA-seq analysis of barcoded cancer cells extracted from lung metastases and immune cells revealed an anti-inflammatory tumor associated macrophage (TAM) cluster expressing high triggering receptor expressed on macrophages 2 (Trem2) program genes and complement component q (C1q).
• 🔗 查看原文

9. GSE337193 新辅助 Botensilimab/Balstilimab 治疗局部错配修复功能正常和缺陷的结肠癌：NEST II 期临床试验结果

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Contributors : Manish A Shah ; Erika Hissong ; Mehraneh D Jafari ; Pashtoon Murtaza Kasi ; Maider Astorkia ; Sahrish Khan ; Casey Owens ; Zhengming Chen ; Heather Yeo ; Fabio Socciarelli ; Sandipto Sarkar ; Alana Nguyen ; Despina Siolas ; Allyson Ocean ; Kelly Garrett ; Lea Lowenfeld ; Alessio Pigazzi ; Preethi Guniganti ; Sanjay Patel ; Doron Betel ; Manuel HidalgoSeries Type : OtherOrganism : Homo sapiensPurpose: Effective immunotherapy for mismatch repair proficient colorectal cancer (CRC) is lacking. We examined the safety and efficacy of the novel next-generation immune activator/Fc-enhanced CTLA-4 inhibitor botensilimab (BOT) plus PD-1 inhibitor balstilimab (BAL) in the neoadjuvant setting for patients with resectable CRC.Patients and Methods: Patients 18 years of age or older with non-metastatic CRC awaiting surgical resection were eligible. BOT/BAL was administered followed by surgical resection. In cohort A, patients received BOT 75 mg d1 and BAL 240 mg d1, 15; in cohorts B/C, patients received 2 additional BAL doses (d29, 43). The primary study objectives were safety, feasibility (based on surgery delay), and pathologic response. Exploratory analyses examined changes in the tumor microenvironment.Results: Twenty-four eligible patients (26 tumors, n=22 pMMR; n=4 dMMR) were enrolled (two patients had synchronous primary tumors). Neoadjuvant BOT/BAL was safe and did not delay planned surgery in any patient. The major pathologic response rate was 41% (95% CI, 21%–64%) for pMMR, and 100% (95% CI, 40%–100%) for dMMR CRC. BOT/BAL was associated with significant anti-tumor effects in the tumor microenvironment, with an increase in the density and proportion of CD8+ T cells, a reduction in tumor infiltrating FOXP3+ Tregs, and evidence of increased immune cell-cell interaction in responding patients.Conclusions: These findings demonstrate safety, feasibility, and encouraging pathological responses for BOT/BAL in both non-metastatic pMMR and dMMR CRC. Tumor microenvironment remodeling suggests a robust anti-tumor immune response induced by immunotherapy. These data support the continued development of BOT/BAL in CRC.
• 🔗 查看原文

10. GSE337095 远程缺血预适应通过减轻肺部炎症和纤维化来缓解柴油机尾气引起的肺纤维化

• ✍️ 作者：未知作者
• 🏷️ 关键词：inflammation
• 📝 描述：Contributors : Kumari Swati ; Mishra Kaushlendra ; Singh Kanika ; Kumar G Krishna ; Kumar Rohit ; Parihar Abhishek ; Kumari Rani ; Nagar Ekta ; Arora Naveen ; Bansal Vishal ; Mishra AasthaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusRemote ischemic conditioning (RIC), a promising non-pharmacological strategy, mitigates ischemia-reperfusion injury in multiple conditions. However, its potential as a therapeutic strategy in pulmonary fibrosis (PF), which is associated with poor survival outcomes and limited pharmacotherapies, remains unexplored. Thus, the present study aimed to explore the therapeutic utility of RIC in a diesel exhaust-induced mouse model recapitulating PF pathology. The effects of RIC were comprehensively assessed by measuring physiological and molecular endpoints, including weight, heart rate variability (HRV), lung histopathology, circulating plasma biomarkers, bronchoalveolar lavage fluid cytokine profiles, and indices of pulmonary mitochondrial health, accompanied by bulk transcriptome sequencing of lung tissue of age-matched mice allocated to nine study groups. DE-induced PF groups receiving RIC showed marked improvements in weight, HRV, and lung mitochondrial function, substantial attenuation of histopathological damage, and significant decreases in inflammatory and fibrotic markers, indicating the potential of RIC to mitigate DE-induced fibrotic damage. Transcriptome analysis of lung tissue further supported these observations by revealing downregulation of PF markers in RIC-receiving groups, highlighting RIC’s efficacy in attenuating PF features. Additionally, potential PF-relevant markers, including Calhm6, Hrc, Dnase1l3, Pla2g2d, Stab2 and Timd4, were identified across these groups that may be linked to therapeutic benefits conferred by RIC.
• 🔗 查看原文

💡 该来源还有 3 条内容，详见 文末

详细内容（全部2条）

1. 科学家发现为何有些人的大脑能够抵抗阿尔茨海默病。

• ✍️ 作者：未知作者
• 🏷️ 关键词：Alzheimer
• 📝 描述：Some brains appear to fight back against Alzheimer’s by helping immature brain cells survive damage instead of succumbing to it. Understanding this natural resilience could point researchers toward entirely new ways to protect memory and slow dementia.
• 🔗 查看原文

2. 健康老龄化的秘密可能就隐藏在你的血液里。

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Some people live past 100 with remarkable health, and researchers may have uncovered one reason why. A new study found that centenarians have a unique chemical “fingerprint” in their blood that sets them apart from normal aging, including unusual patterns of bile acids and steroids linked to longer survival.
• 🔗 查看原文

• GSE337062 感染 IHNV 的虹鳟脾脏和过表达 circUSP47 的虹鳟肝细胞的 circRNA、miRNA 和 mRNA 转录组综合分析。
• GSE303713 发育过程中不同的肺泡巨噬细胞介导香烟烟雾引起的炎症和损伤
• GSE271950 HN1介导的AKT-SREBP信号通路激活脂肪生成促进肝细胞癌细胞增殖和转移