详细内容（前10条）

1. ⭐ GSE295967 单细胞多组学揭示 KRAS 抑制后肿瘤和免疫重塑的协调性 [空间转录组学]

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、single-cell、spatial、spatial transcriptomics、transcriptomics、KRAS
• 📝 描述：Contributors : Daniel R Lu ; Tracy M Yamawaki ; Shining Ma ; Chi-Ming LiSeries Type : OtherOrganism : Mus musculusAllele-specific KRAS inhibitors are approved for KRAS-mutant cancers, and efforts to maximize patient responses are ongoing. Previous work suggests that KRAS inhibition can modulate anti-tumor immunity, but the critical cellular players and molecular pathways have not been clearly defined. Here we perform multi-omic profiling using single-cell RNA-seq, spatial transcriptomics, and flow cytometry to profile intratumoral changes after KRASG12C inhibition (KRAS(G12C)i), MEK inhibition (MEKi), and combination therapy with KRAS(G12C)i and PD-1 blockade. Treatment with KRAS(G12C)i but not MEKi drove neoplastic adaptation toward increased oxidative stress, integrin signaling, and upregulation of extracellular matrix remodeling factors. These changes were accompanied by changes in spatial intercellular communication patterns, leading to robust cDC maturation and CD8 effector T-cell activation. Combination treatment with anti-PD-1 further expands T-cell responses, as well as macrophage states associated with increased cancer survival. Our findings connect KRAS inhibition with the coordination of subsequent immune activation and reveal molecular mechanisms that promote and sustain tumor control.
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2. ⭐ GSE335761 人类衰老肺实质细胞时钟空间转录组学数据

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Patty J Lee ; So-Jin Kim ; Alter Klausner ; Alexander M Tsankov ; Ke Xu ; Atharva Bhagwat ; Vignesh V VenkatSeries Type : OtherOrganism : Homo sapiensThis Xenium spatial transcriptomics data from normal lung samples of different ages used in the study Human Cellular Clock of the Aging Lung Parenchyma.
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3. ⭐ GSE335372 CAR-T药物偶联物抗实体瘤[空间转录组学]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Shan Xin ; Yanzhi Feng ; Feifei Zhang ; Chuanpeng Dong ; Sidi ChenSeries Type : OtherOrganism : Homo sapiensChimeric antigen receptor (CAR)-T cell therapy has been clinically successful in hematologic malignancies but faces challenges in solid tumors, including limited infiltration, immunosuppressive microenvironments and antigen heterogeneity. While combining CAR-T cell therapy with chemotherapy can enhance antitumor activity, this often leads to substantial systemic toxicity. Here we show that CAR-T-drug conjugate (CAR-T-D-C), generated through click chemistry-mediated conjugation of cytotoxic payloads to antigen-specific CAR-T cells, enable localized drug delivery while preserving CAR-T cell function. CAR-T-D-Cs incorporating different CAR-T cell binders exhibit robust antitumor activity across multiple human xenografts and syngeneic tumor models. Spatial transcriptomic analyses reveal enhanced intratumoral CAR-T infiltration and activation following CAR-T-D-C treatment . Compared with conventional CAR-T therapy, CAR-T-D-C enhances immune cell infiltration, augments effector functions, promotes antigen spreading and amplifies systemic anti-tumor immunity. CAR-T-D-C represents a versatile therapeutic platform that combines the specificity of cellular immunotherapy with the potency of small-molecule therapeutics for treatment of solid tumors.
• 🔗 查看原文

4. ⭐ GSE295965 单细胞多组学揭示 KRAS 抑制后肿瘤和免疫重塑的协调性 [scRNA-Seq 1]

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、single-cell、scRNA、KRAS
• 📝 描述：Contributors : Daniel R Lu ; Tracy M Yamawaki ; Shining Ma ; Chi-Ming LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAllele-specific KRAS inhibitors are approved for KRAS-mutant cancers, and efforts to maximize patient responses are ongoing. Previous work suggests that KRAS inhibition can modulate anti-tumor immunity, but the critical cellular players and molecular pathways have not been clearly defined. Here we perform multi-omic profiling using single-cell RNA-seq, spatial transcriptomics, and flow cytometry to profile intratumoral changes after KRASG12C inhibition (KRAS(G12C)i), MEK inhibition (MEKi), and combination therapy with KRAS(G12C)i and PD-1 blockade. Treatment with KRAS(G12C)i but not MEKi drove neoplastic adaptation toward increased oxidative stress, integrin signaling, and upregulation of extracellular matrix remodeling factors. These changes were accompanied by enhanced intercellular communication with classical dendritic cells, leading to robust cDC maturation and CD8 effector T-cell activation. Combination treatment with anti-PD-1 further expands T-cell responses, as well as macrophage states associated with increased cancer survival. Our findings connect KRAS inhibition with the coordination of subsequent immune activation and reveal molecular mechanisms that promote and sustain tumor control.
• 🔗 查看原文

5. ⭐ GSE320221 整合细针穿刺和单细胞RNA测序技术研究代谢功能障碍相关脂肪肝疾病

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolic、sequencing、single-cell
• 📝 描述：Contributors : Ana C Maretti-Mira ; Matthew P Salomon ; Lucy Golden-MasonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMetabolic dysfunction-associated steatotic liver disease (MASLD) is currently the leading cause of chronic liver disease and hepatocellular carcinoma. The immune response plays a central role in disease onset and progression and is the focus of many experimental studies. However, traditional models typically rely on terminal sampling procedures that require large tissue quantities, substantial numbers of animals per experimental condition, and cross-sectional study designs. Here, we propose the integration of two powerful techniques to longitudinally study a MASLD animal model: image-guided fine-needle aspiration (FNA) and single-cell RNA sequencing (scRNA-seq).
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6. ⭐ GSE295974 单细胞多组学揭示 KRAS 抑制后肿瘤和免疫重塑的协调性

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、single-cell、KRAS
• 📝 描述：Contributors : Daniel R Lu ; Tracy M Yamawaki ; Shining Ma ; Chi-Ming LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAllele-specific KRAS inhibitors are approved for KRAS-mutant cancers, and efforts to maximize patient responses are ongoing. Previous work suggests that KRAS inhibition can modulate anti-tumor immunity, but the critical cellular players and molecular pathways have not been clearly defined. Here we perform multi-omic profiling using single-cell RNA-seq, spatial transcriptomics, and flow cytometry to profile intratumoral changes after KRASG12C inhibition (KRAS(G12C)i), MEK inhibition (MEKi), and combination therapy with KRAS(G12C)i and PD-1 blockade. Treatment with KRAS(G12C)i but not MEKi drove neoplastic adaptation toward increased oxidative stress, integrin signaling, and upregulation of extracellular matrix remodeling factors. These changes were accompanied by enhanced intercellular communication with classical dendritic cells, leading to robust cDC maturation and CD8 effector T-cell activation. Combination treatment with anti-PD-1 further expands T-cell responses, as well as macrophage states associated with increased cancer survival. Our findings connect KRAS inhibition with the coordination of subsequent immune activation and reveal molecular mechanisms that promote and sustain tumor control.
• 🔗 查看原文

7. GSE313404 单细胞 RNA 测序揭示共生普雷沃氏菌如何通过重编程髓系细胞增强机体对肺炎链球菌肺部感染的防御能力

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Contributors : Sara N Stoner ; Eric D Larson ; Sam Fulte ; Erin R Fish ; Steve C Shaw ; Edward N Janoff ; Matthias Mack ; Sarah E ClarkSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusStreptococcus pneumoniae expresses a suite of virulence factors that modulate the innate immune response, delaying effective clearance. However, immune interactions with other bacteria influence this dynamic. Depletion of respiratory tract anaerobes including Prevotella is associated with increased mortality from pneumonia, an effect that can be replicated by pre-exposure to Prevotella melaninogenica in mice. Here, we demonstrate using single-cell RNA sequencing that P. melaninogenica shifts myeloid cell transcriptional profiles during pneumococcal infection from interferon-dominant to pro-phagocytic, correlating with increased S. pneumoniae phagocytosis and clearance from the lung. In neutrophils, improved antibacterial defense was dependent on TNF signaling through TNFR2. Prevotella-enhanced defense also required the recruitment of monocyte-derived macrophages, with selective enrichment of a Cxcl3+ population which differed from the C1qa+ population dominant during S. pneumoniae infection in unprimed mice. This work reveals an optimized innate immune defense program which may contribute to natural infection resistance mediated by respiratory tract commensal-host interactions.
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8. GSE335371 CAR-T药物偶联物抗实体瘤[RNA-seq/BCR-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、RNA-seq
• 📝 描述：Contributors : Shan Xin ; Yanzhi Feng ; Feifei Zhang ; Chuanpeng Dong ; Sidi ChenSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiens ; Mus musculusChimeric antigen receptor (CAR)-T cell therapy has been clinically successful in hematologic malignancies but faces challenges in solid tumors, including limited infiltration, immunosuppressive microenvironments and antigen heterogeneity. While combining CAR-T cell therapy with chemotherapy can enhance antitumor activity, this often leads to substantial systemic toxicity. Here we show that CAR-T-drug conjugate (CAR-T-D-C), generated through click chemistry-mediated conjugation of cytotoxic payloads to antigen-specific CAR-T cells, enable localized drug delivery while preserving CAR-T cell function. CAR-T-D-Cs incorporating different CAR-T cell binders exhibit robust antitumor activity across multiple human xenografts and syngeneic tumor models. Spatial transcriptomic analyses reveal enhanced intratumoral CAR-T infiltration and activation following CAR-T-D-C treatment . Compared with conventional CAR-T therapy, CAR-T-D-C enhances immune cell infiltration, augments effector functions, promotes antigen spreading and amplifies systemic anti-tumor immunity. CAR-T-D-C represents a versatile therapeutic platform that combines the specificity of cellular immunotherapy with the potency of small-molecule therapeutics for treatment of solid tumors.
• 🔗 查看原文

9. GSE336305 全基因组 CRISPR 筛选细胞毒性 T 淋巴细胞介导的细胞杀伤作用，该作用独立于 Casp3、Mlkl 和 Bid

• ✍️ 作者：未知作者
• 🏷️ 关键词：genome
• 📝 描述：Contributors : Youxian Li ; Shixiong WangSeries Type : OtherOrganism : Mus musculusTo gain insight into cytotoxic lymphocyte–mediated, Casp3, necroptosis, and Mitochondrial outer membrane permeabilization (MOMP) independent cancer cell death, we leveraged the a CTL–αCD3–P815 killing system and performed a genome-wide CRISPR screen. Cas9 expressing and Casp3-/-Mlkl-/-Bid-/- mouse mastocytoma P815 cells were first transduced with Brie gRNA library and subjected to cytotoxic T lymphocytes-mediated selection. Surviving cells were collected and sequenced to identify genes/gRNAs enriched following selection.
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10. GSE336303 全基因组 CRISPR 筛选细胞毒性 T 淋巴细胞介导的、不依赖于 Casp3 的细胞杀伤作用

• ✍️ 作者：未知作者
• 🏷️ 关键词：genome
• 📝 描述：Contributors : Youxian Li ; Shixiong WangSeries Type : OtherOrganism : Mus musculusTo gain insight into cytotoxic lymphocyte–mediated, Casp3-independent cancer cell death, we leveraged the a CTL–αCD3–P815 killing system and performed a genome-wide CRISPR screen. Cas9 expressing and Casp3-/- mouse mastocytoma P815 cells were first transduced with Brie gRNA library and subjected to cytotoxic T lymphocytes-mediated selection. Surviving cells were collected and sequenced to identify genes/gRNAs enriched following selection.
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详细内容（全部2条）

1. 全RNA测序提高了急性白血病中基因融合的检测率

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、sequencing
• 📝 描述：RNA sequencing enabled comprehensive detection of clinically relevant gene fusions in acute leukemia, including rare rearrangements that conventional diagnostic methods failed to identify… The post Whole RNA sequencing improves gene fusion detection in acute leukemia appeared first on RNA-Seq Blog.
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2. 与牛津纳米孔技术公司的合作将支持RNA液体活检技术的发展，用于癌症早期检测。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：RNA sequencing and nanopore sequencing enabled Eclipse-like full transcriptome analysis to identify novel non-coding RNA biomarkers in extracellular vesicles for early esophageal… The post Partnership with Oxford Nanopore Technologies will support development of RNA liquid biopsy for cancer early detection appeared first on RNA-Seq Blog.
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• GSE296733 高浓度臭氧通过IgG糖基化重编程激活母体免疫，从而引发子代IgG介导的过敏反应
• GSE335592 CAR-T药物偶联物抗实体瘤
• GSE327101 单细胞多模态图谱揭示人脑中的调控程序
• GSE326778 HR阳性/HER2阴性乳腺癌的分期相关转录变化
• GSE326255 姜黄素和萝卜硫素通过独特而互补的转录特征维持衰老秀丽隐杆线虫的运动能力
• GSE278526 人类脂肪组织中肥胖和 II 型糖尿病的转录和细胞学探索 [scRNA-seq]
• GSE269909 髓母细胞瘤染色质状态差异揭示 KDM2B 是一种选择性依赖性 [RNA-Seq]
• GSE336337 铜绿假单胞菌 LysR 型转录调节因子 PA1290 对生物膜的形成至关重要，并影响毒力和致病性 [RNA-seq]
• GSE336126 DNA修饰与增强子特征在退出原始多能性过程中的双重分析[RNA-Seq]
• GSE336124 原始多能性退出过程中具有增强子特征的 DNA 修饰的双重分析 [hMethyl-ATAC-seq]
• GSE314081 组蛋白变体 H1.0 通过相分离介导的染色质组织调控终末红系分化
• GSE299917 RNA-seq 分析野生型 Xenorhabdus griffiniae HGB2511 暴露于常规和无菌 Steinernema hermaphroditum 线虫。
• GSE336440 膳食脂肪来源方案在灵长类动物模型中表现出不同的血浆microRNA特征和代谢及认知轨迹
• GSE319926 SERIPH：一种用于选择性富集半可提取RNA的两步提取方案