详细内容（全部6条）

1. ⭐ GSE306321 单细胞 RNA 测序揭示了 PDAC 肿瘤微环境中 Lin28b 依赖的免疫调节

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immune、tumor microenvironment、sequencing、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCancer-associated fibroblasts (CAFs) play a crucial role in shaping the pancreatic ductal adenocarcinoma (PDAC) tumor microenvironment (TME). This study investigates the impact of an RNA-binding protein Lin28b on the immune landscape of PDAC. Single-cell RNA sequencing (scRNA-seq) was performed on orthotopic pancreatic tumors from wild-type (WT) and fibroblast-specific Lin28b knockout (Fsp-Cre;Lin28bfl/fl) mice. Our analysis revealed that Lin28b expression in CAFs promotes an immunosuppressive TME characterized by reduced immune cell infiltration and dampened type I interferon (IFN) signaling. Conversely, Lin28b deletion in CAFs resulted in increased infiltration of immune cells, particularly CD8+ T cells and enhanced cytotoxic T cell activity. These findings identify Lin28b as a CAF-intrinsic molecular brake on anti-tumor immunity and provide a rationale for targeting the Lin28 to overcome PDAC resistance to immunotherapy.
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2. ⭐ GSE335539 ​​DNAJC2敲低透明细胞肾细胞癌细胞的高通量转录组测序（shDNAJC2 vs shCtrl）

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、sequencing、transcriptome
• 📝 描述：Contributor : Yunkai YangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis project was designed to characterize transcriptomic changes caused by DNAJC2 knockdown in clear cell renal cell carcinoma (ccRCC) cells using high-throughput RNA sequencing. Cells transduced with control shRNA and cells with DNAJC2 knockdown were subjected to next-generation transcriptome sequencing to identify differentially expressed genes and pathways regulated by DNAJC2. This dataset was used to investigate the role of DNAJC2 in ccRCC cell metabolism and ferroptosis-related regulation
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3. GSE306320 RNA免疫沉淀测序鉴定出PDAC癌相关成纤维细胞中Lin28b的RNA靶标

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、sequencing
• 📝 描述：Series Type : OtherOrganism : Mus musculusThis study uses RNA immunoprecipitation followed by sequencing (RIP-seq) to identify RNA targets of Lin28b in PDAC cancer-associated fibroblasts (CAFs). CAFs expressing either Flag-wild-type Lin28b (Lin28b-WT) or an RNA-binding deficient mutant (Flag-Lin28b-MU) were subjected to RIP-seq. Our analysis revealed that STING mRNA, a key component of the innate immune cGAS-STING pathway, is a direct target of Lin28b. This interaction leads to decreased STING mRNA stability and reduced STING protein levels, thereby suppressing type I interferon (IFN) production. These findings provide a mechanistic explanation for the immunosuppressive function of Lin28b in the PDAC tumor microenvironment.
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4. GSE303717 Nodal/Smad2信号通路通过抑制Pparg介导的脂质代谢来维持发育停滞

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism
• 📝 描述：Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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5. GSE335752 人类 M2 样（TGFβ/IL10）与 M0 单核细胞衍生巨噬细胞的特征分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：monocyte
• 📝 描述：Contributors : Jennifer Yinuo Cao ; Giovanni C ForcinaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPeripheral human monocytes from different human donors were differentiated into M2-like (TGFbeta/IL10) or M0 and analyzed by RNAseq.
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6. GSE297633 IRE1活性调控肿瘤和微环境细胞谱系状态，同时对局限性和转移性前列腺癌进行分层

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Contributors : Dimitrios Doultsinos ; Ian Mills ; Ingrid Tomljanovic ; Eleftherios Pilalis ; Aristotelis ChatziioannouSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensProstate cancer (PCa) is an androgen receptor (AR) driven, high-incidence disease significantly contributing to cancer mortality. PCa is in need of better risk stratification at diagnosis and treatment outcomes in patients at high risk of metastasis. The unfolded protein response (UPR) is an AR-dependent process. However, the impact of the UPR transducer IRE1 on AR-dependent biology and treatment resistance has not been defined. We use diverse pre-clinical models of stress response to describe IRE1 activity impact on multiple disease stages and demonstrate its involvement with poor prognosis (RB1 loss), and cell lineage determination (club phenotypes). Integrating clinical transcriptomic datasets, we chart IRE1 activity throughout PCa evolution by developing a PCa-specific, IRE1 activity gene set (IRE1_18) reflecting both tumoral and micro-environmental niches. IRE1_18 can determine tumoral identity, inform androgen deprivation treatment suitability, prognosticate localised and metastatic disease independently from AR activity, and guide IRE1 modulation as a novel combination therapeutic.
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详细内容（全部1条）

1. 这种DNA修复基因失控，暴露了癌症的弱点。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have discovered that a gene normally considered a DNA-protecting “good guy” can become dangerous when cells make too much of it. The gene, EXO1, acts like molecular scissors that help repair DNA, but when overproduced it starts cutting DNA it shouldn’t, creating damage linked to cancer.
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