详细内容（全部10条）

1. ⭐ GSE305085 致癌 Ras 信号通过下调 EED 来破坏 PRC2，从而驱动鳞状细胞癌中的致癌重编程和微环境重塑 [小鼠 RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、regex:onco(logy|logist|gene|genic)、RNA-seq
• 📝 描述：Contributors : Meng-Yen Li ; Xiang Yu Zheng ; Deyou Zheng ; Elena EzhkovaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSquamous cell carcinoma (SCC) is a common epithelial malignancy, yet its epigenetic underpinnings remain poorly defined. Here, we identify the Polycomb Repressive Complex 2 (PRC2) as a key epigenetic barrier of SCC tumorigenesis. Despite high EZH2 levels, the PRC2-dependent H3K27me3 is depleted in human and murine SCCs, indicating complex destabilization rather than enzymatic failure. Mechanistically, oncogenic Ras signaling downregulates the PRC2 scaffolding subunit EED, disrupting PRC2 and H3K27me3 and activating oncogenic loci. Depletion of Eed in epidermal progenitors disrupts the proliferation–differentiation balance and, upon carcinogen exposure, drives invasive SCC. Single-cell RNA-seq of Eed-deficient tumors reveals an expansion of tumor-specific keratinocytes and a remodeled pro-tumorigenic microenvironment. Notably, reintroducing EED in SCC cells restores PRC2 integrity and H3K27me3 levels, suppressing tumor growth and mitigating the pro-tumorigenic niche. Our findings establish PRC2 as an essential epigenetic gatekeeper in SCC and suggest restoring PRC2-mediated repression may offer a novel therapeutic strategy.
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2. GSE305084 致癌 Ras 信号通过下调 EED 来破坏 PRC2，从而驱动鳞状细胞癌中的致癌重编程和微环境重塑 [小鼠 CUT & RUN]

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、regex:onco(logy|logist|gene|genic)
• 📝 描述：Contributors : Meng-Yen Li ; Xiang Yu Zheng ; Deyou Zheng ; Elena EzhkovaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusSquamous cell carcinoma (SCC) is a common epithelial malignancy, yet its epigenetic underpinnings remain poorly defined. Here, we identify the Polycomb Repressive Complex 2 (PRC2) as a key epigenetic barrier of SCC tumorigenesis. Despite high EZH2 levels, the PRC2-dependent H3K27me3 is depleted in human and murine SCCs, indicating complex destabilization rather than enzymatic failure. Mechanistically, oncogenic Ras signaling downregulates the PRC2 scaffolding subunit EED, disrupting PRC2 and H3K27me3 and activating oncogenic loci. Depletion of Eed in epidermal progenitors disrupts the proliferation–differentiation balance and, upon carcinogen exposure, drives invasive SCC. Single-cell RNA-seq of Eed-deficient tumors reveals an expansion of tumor-specific keratinocytes and a remodeled pro-tumorigenic microenvironment. Notably, reintroducing EED in SCC cells restores PRC2 integrity and H3K27me3 levels, suppressing tumor growth and mitigating the pro-tumorigenic niche. Our findings establish PRC2 as an essential epigenetic gatekeeper in SCC and suggest restoring PRC2-mediated repression may offer a novel therapeutic strategy.
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3. GSE305083 致癌 Ras 信号通过下调 EED 来破坏 PRC2，从而驱动鳞状细胞癌中的致癌重编程和微环境重塑 [人类 CUT & RUN]

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、regex:onco(logy|logist|gene|genic)
• 📝 描述：Contributors : Meng-Yen Li ; Xiang Yu Zheng ; Deyou Zheng ; Elena EzhkovaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensSquamous cell carcinoma (SCC) is a common epithelial malignancy, yet its epigenetic underpinnings remain poorly defined. Here, we identify the Polycomb Repressive Complex 2 (PRC2) as a key epigenetic barrier of SCC tumorigenesis. Despite high EZH2 levels, the PRC2-dependent H3K27me3 is depleted in human and murine SCCs, indicating complex destabilization rather than enzymatic failure. Mechanistically, oncogenic Ras signaling downregulates the PRC2 scaffolding subunit EED, disrupting PRC2 and H3K27me3 and activating oncogenic loci. Depletion of Eed in epidermal progenitors disrupts the proliferation–differentiation balance and, upon carcinogen exposure, drives invasive SCC. Single-cell RNA-seq of Eed-deficient tumors reveals an expansion of tumor-specific keratinocytes and a remodeled pro-tumorigenic microenvironment. Notably, reintroducing EED in SCC cells restores PRC2 integrity and H3K27me3 levels, suppressing tumor growth and mitigating the pro-tumorigenic niche. Our findings establish PRC2 as an essential epigenetic gatekeeper in SCC and suggest restoring PRC2-mediated repression may offer a novel therapeutic strategy.
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4. GSE305735 组织中单个细胞的全转录组规模异构体分辨空间成像

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、transcriptome
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCell and tissue functions arise from complex interactions among numerous genes, and a systematic understanding of these functions requires isoform-resolved transcriptomic analysis of single cells with high spatial resolution. Here, we introduce an in situ RNA amplification method and its integration with multiplexed error-robust fluorescence in situ hybridization (MERFISH) to detect short RNA sequences and enable isoform-resolved spatial transcriptomics of individual cells in intact tissues. Using this approach, we imaged ~33,000 distinct RNAs—including ~23,000 genes and ~10,000 isoforms—in the mouse brain. Our data enabled systematic analyses of region- and cell-type-specific gene programs and ligand-receptor-based cell-cell communications. These data further revealed rich spatial diversity and cell-type specificity in isoform usage across numerous genes, as well as brain structures particularly rich in specific isoform usage. We anticipate broad application of this method for characterizing molecular and cellular basis of tissue functions, unlocking previously inaccessible discoveries in cell and organismal biology.
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5. GSE304812 致癌Ras信号通过下调EED破坏PRC2，从而驱动鳞状细胞癌中的致癌重编程和微环境重塑

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、regex:onco(logy|logist|gene|genic)
• 📝 描述：Contributors : Meng-Yen Li ; Aubrey Houser ; Paula Restrepo ; Andrew Ji ; Elena EzhkovaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSquamous cell carcinoma (SCC) is a common epithelial malignancy, yet its epigenetic underpinnings remain poorly defined. Here, we identify the Polycomb Repressive Complex 2 (PRC2) as a key epigenetic barrier of SCC tumorigenesis. Despite high EZH2 levels, the PRC2-dependent H3K27me3 is depleted in human and murine SCCs, indicating complex destabilization rather than enzymatic failure. Mechanistically, oncogenic Ras signaling downregulates the PRC2 scaffolding subunit EED, disrupting PRC2 and H3K27me3 and activating oncogenic loci. Depletion of Eed in epidermal progenitors disrupts the proliferation–differentiation balance and, upon carcinogen exposure, drives invasive SCC. Single-cell RNA-seq of Eed-deficient tumors reveals an expansion of tumor-specific keratinocytes and a remodeled pro-tumorigenic microenvironment. Notably, reintroducing EED in SCC cells restores PRC2 integrity and H3K27me3 levels, suppressing tumor growth and mitigating the pro-tumorigenic niche. Our findings establish PRC2 as an essential epigenetic gatekeeper in SCC and suggest restoring PRC2-mediated repression may offer a novel therapeutic strategy.
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6. GSE281706 即使在缺乏成熟脂肪细胞的情况下，大网膜对卵巢肿瘤细胞生长的支持作用也不会减弱

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor
• 📝 描述：Contributors : Rachel L Mintz ; Emily G Butka ; Alexandre Gallerand ; Jichang Han ; Ayoung Kim ; Sarah Ning ; Nicole Yiew ; Mary Wohltmann ; Wei Zou ; Samantha A Morris ; Bernd H Zinselmeyer ; Gwendalyn J RandolphSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusThe omentum, a specialized adipose tissue arising off the stomach, is a key premetastatic niche for ovarian cancer metastasis and growth within the peritoneum. Why the omentum promotes optimal ovarian cancer growth remains unclear, but the prevailing concept is that omental adipocytes and the accompanying metabolic exchange of FABP4-binding fatty acids are critical. Here, we generated mice lacking mature adipocytes in all peritoneum tissues, including the omentum. ID8p53–/–Brca2–/– tumors retained a propensity to seed at regions typically associated with adipose depots, even without mature adipocytes. However, the lack of mature adipocytes did not suppress tumor expansion in the peritoneum, whereas removing the adipocyte-free omentum did. Single-cell RNA sequencing analysis revealed that endothelial FABP4 was especially high in the omentum, potentially accounting for the role of FABP4 in tumor growth rather than adipocyte FABP4, as previously thought. Thus, whether and how tumor cell-adjacent adipocytes impact tumor progression needs re-evaluation
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7. GSE331505 工程化人源PEG10基纳米颗粒用于RNA自包装、递送和癌症治疗

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Contributors : Pin-Yan Chen ; Ting-Lun Tien ; Vy Anh Truong ; Quyen Thuc Dang ; Nuong Thi Kieu Nguyen ; Pin-Hsin Chen ; Yu-Chen HuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensEngineered protein-based protein nanoparticles (PBNPs) were developed for RNA self-packaging and delivery. To characterize the RNA cargo composition preferentially encapsulated within PEG10 PBNPs, RNA sequencing was performed on purified nanoparticle samples and producer cells. This dataset provides transcriptomic profiling of packaged RNAs associated with PEG10-mediated RNA delivery systems.
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8. GSE334803 溃疡性结肠炎患者和健康对照者的结肠组织 RNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Contributors : Mengqi Zheng ; Shiyang LiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA sequencing was conducted on colonic biopsy tissues obtained from patients with ulcerative colitis and human healthy controls (n = 6 per group).
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9. GSE334799 HeLa-NXF1-dTAG 细胞在 NXF1 降解后的 3’ 末端测序，以表征替代性多聚腺苷酸化动力学

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Series Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensNuclear export factor 1 (NXF1) is the principal mRNA export receptor in eukaryotes. To characterize the role of NXF1 in regulating alternative polyadenylation (APA) dynamics, we employed the dTAG degradation system in a HeLa-NXF1-dTAG stable cell line. Cells were treated with 500 nM dTAG-13 or DMSO (vehicle control) for 6 hours to induce acute NXF1 depletion. Total RNA was subjected to 3’ end sequencing (QuantSeq 3’ mRNA-Seq Library Prep Kit V2, Lexogen) for APA analysis. Each condition was performed in biological duplicate.
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10. GSE318009 XPO1抑制诱导NCOR1依赖性氧化应激以抑制肝细胞癌

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma
• 📝 描述：Contributors : Wei Wang ; Linmeng WangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHepatocellular carcinoma (HCC) remains a therapeutic challenge due to the limited efficacy of current systemic therapies. To identify potential novel therapeutic approaches, computational analysis of HCC datasets and drug screening were integrated, leading to the repurposing of hematological cancer drug selinexor (an XPO1 inhibitor) for HCC treatment. Functional studies revealed that XPO1 inhibition triggers oxidative stress and cell cycle arrest in HCC cells through nuclear sequestration of NCOR1, disrupting redox homeostasis through FOXK1-dependent transcriptional activation of genes associated with Reactive Oxygen Species (ROS). A genome-wide CRISPR-Cas9 screen further identified the KEAP1-NRF2 axis as a key determinant of sensitivity to XPO1 inhibition. Furthermore, high-throughput compound screening demonstrated that disulfiram, a clinically-used aldehyde dehydrogenase inhibitor, synergizes with XPO1 inhibitor through exacerbation of ROS accumulation. Collectively, these findings demonstrate the therapeutic repurposing of selinexor for HCC while uncovering its novel mechanism of action, establishing a predictive biomarker, and proposing an immediately translatable combination therapy.
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详细内容（全部2条）

1. 新型芬太尼疫苗可在致命过量用药发生前将其阻断。

• ✍️ 作者：未知作者
• 🏷️ 关键词：vaccine
• 📝 描述：A new experimental vaccine developed by Scripps Research could offer a powerful new way to prevent fentanyl overdoses by stopping the drug before it reaches the brain. Rather than targeting only fentanyl itself, the vaccine trains the immune system to recognize a broad range of fentanyl-related designer drugs, including some of the most dangerous variants.
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2. 古代丹尼索瓦人的DNA至今仍然影响着人类的免疫力。

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity
• 📝 描述：Ancient encounters between humans and the mysterious Denisovans are still shaping people today. By analyzing genomes from populations across the Pacific, researchers uncovered evidence that the ancestors of Near Oceanians interbred with at least three different Denisovan groups, leaving behind genetic variants that remain active in modern humans.
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