详细内容（前10条）

1. ⭐ GSE333173 生酮饮食通过肠道微生物群介导的JAK2/STAT3/RORγt/IL-17A信号通路减轻急性放射引起的肠道损伤

• ✍️ 作者：未知作者
• 🏷️ 关键词：pathway、gut、regex:gut(-?microbiome)?、regex:intestin(e|al)
• 📝 描述：Contributors : Jingjing Yang ; Zhi Ling ; Ming Zhou ; Mingyang Tao ; Jingxian Mao ; Yefei Zhu ; Xuebing YanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis study demonstrates that ketogenic diet (KD), a high-fat and low-carbohydrate dietary regimen, exerts protective effects against RIII through dual mechanisms involving microbial regulation and inflammatory pathway inhibition.
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2. ⭐ GSE333952 单核细胞中的基线干扰素信号传导和注射部位抗体介导的先天激活影响 mRNA 疫苗的反应原性 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：vaccine、antibody、ATAC-seq
• 📝 描述：Contributors : Natacha Madelon ; Gustavo A Ruiz Buendía ; Arnaud M DidierlaurentSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensThe local and systemic symptoms that follow vaccination -collectively referred to as reactogenicity- are common, yet the mechanisms underlying individual variability remain poorly understood. Through longitudinal immune profiling of vaccinated individuals and mechanistic studies in mice, we identified key immunological determinants of reactogenicity induced by mRNA vaccines. Systemic adverse events were associated with stronger interferon and pro-inflammatory responses following the second dose of COVID-19 vaccine, which were also correlated with the magnitude of the antigen-specific adaptive responses. This heightened inflammation occurred within 24 hours of vaccination and originated primarily from the injection site and was characterized by enhanced recruitment and activation of myeloid cells, particularly monocytes. Two mechanisms contributed to this response: (1) early interferon production by muscle T cells generated after the first dose and (2) FcγR-dependent chemokine induction by antigen-specific antibodies. Consistently, serum antibody levels prior to vaccination correlated positively with reactogenicity. In addition to this local amplification mechanism, variability in reactogenicity was influenced by the baseline immune state, as individuals with a pre-existing interferon-stimulated gene signature in monocytes, detectable at both transcriptomic and epigenetic levels, were more prone to systemic symptoms. Our findings reveal molecular and cellular mechanisms driving vaccine reactogenicity, providing a framework for the design of less reactogenic vaccines.
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3. ⭐ GSE319755 细胞毒性 CD39+ 肿瘤相关 NK 细胞对肺癌中的 NKG2A 阻断有反应（10 倍 Xenium）

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、10x
• 📝 描述：Contributors : Wiebke Rackwitz ; Annalena Brüggemann ; Carina A Pawlow ; Evgeny Chichelnitskiy ; Clara Serger ; Alfred Zippelius ; Lavinia Neubert ; Christine FalkSeries Type : OtherOrganism : Homo sapiensNatural killer (NK) cell–targeting immunotherapies are emerging, yet the differentiation and functional states of tumor-infiltrating NK cells remain poorly understood. Using matched single-nucleus RNA and ATAC sequencing of samples from patients with non–small cell lung cancer (NSCLC), we resolved the transcriptional and epigenetic landscape of intratumoral NK cells. We identified two tumor-associated NK (taNK) cell subsets marked by expression of ITGAE (CD103) and ITGA1 (CD49a) that display features of tissue residency and dysfunction while preserving cytotoxic function. Trajectory and regulon analyses revealed an inflammation-driven transition from early granzyme K (GZMK)+ NK cells toward an ENTPD1+ (CD39+) effector state characterized by interferon-stimulated gene (ISG) programs. Functional profiling established CD39+ taNK cells as the dominant cytotoxic NK cell population with superior killing capacity that was further potentiated by NKG2A blockade. This study offers mechanistic insights into NK cell differentiation in NSCLC and establishes CD39+ taNK cells as a targetable effector population for immunotherapy.
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4. ⭐ GSE304741 细胞毒性 CD39+ 肿瘤相关 NK 细胞对肺癌中 NKG2A 阻断的反应 (10X Multiome)

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、10x
• 📝 描述：Contributors : Clara Serger ; Lucas Rebuffet ; Michael T Sandholzer ; Eric Vivier ; Andrea Romagnani ; Alfred ZippeliusSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Homo sapiensNatural killer (NK) cell–targeting immunotherapies are emerging, yet the differentiation and functional states of tumor-infiltrating NK cells remain poorly understood. Using matched single-nucleus RNA and ATAC sequencing of samples from patients with non–small cell lung cancer (NSCLC), we resolved the transcriptional and epigenetic landscape of intratumoral NK cells. We identified two tumor-associated NK (taNK) cell subsets marked by expression of ITGAE (CD103) and ITGA1 (CD49a) that display features of tissue residency and dysfunction while preserving cytotoxic function. Trajectory and regulon analyses revealed an inflammation-driven transition from early granzyme K (GZMK)+ NK cells toward an ENTPD1+ (CD39+) effector state characterized by interferon-stimulated gene (ISG) programs. Functional profiling established CD39+ taNK cells as the dominant cytotoxic NK cell population with superior killing capacity that was further potentiated by NKG2A blockade. This study offers mechanistic insights into NK cell differentiation in NSCLC and establishes CD39+ taNK cells as a targetable effector population for immunotherapy.
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5. ⭐ GSE334067 配对突变检测和空间转录组学鉴定出与小鼠结肠炎肿瘤发生相关的细胞群

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Elisa B Moutin ; Giada Giavara ; Filipe C Lourenco ; Nefeli Skoufou-Papoutsaki ; Douglas WintonSeries Type : OtherOrganism : Mus musculusIn the progression from Inflammatory Bowel Disease to associated cancer, the clonal mutational landscape shifts from selection of mutations in inflammatory genes to selection for cancer-driver mutations. How prevalence and expansion of either type of mutant clones could be impacted by the cellular environments in which they arise, and how this affects the neoplastic outcome of colitis, remains unknown. Here, we combine in vivo lineage tracing, in silico modelling, mutational profiling and spatial transcriptomics in a mouse model of colitis-associated tumorigenesis to capture clone fates associated with chronic inflammation. We identify epithelial- and immune-enriched neighbourhoods and propose a model in which establishment of a reparative tissue environment facilitates tumour initiation by promoting the selection and expansion of pro-oncogenic clones, reducing the span of inflammation-resistant neighbourhoods containing non-oncogenic clones.
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6. GSE334206 STK25 m6A 修饰调节系统性红斑狼疮中 CD4+ T 细胞糖酵解介导的免疫失衡

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、T cell
• 📝 描述：Contributors : Ming Li ; Yunfei Li ; Lijun Pang ; Junjie Chen ; Shuangshuang Shang ; Chuanbing HuangSeries Type : OtherOrganism : Homo sapiensSystemic lupus erythematosus (SLE) is a complex autoimmune disease with an incompletely understood pathogenesis. N6-methyladenosine (m6A) has been implicated in immune regulation and disease progression, yet its role in disrupting immune homeostasis in SLE, particularly in CD4+ T-cell differentiation, remains poorly understood. In the present study, m6A-modified RNA immunoprecipitation sequencing (m6A-seq) and RNA sequencing (RNA-seq) of peripheral blood mononuclear cells from patients with SLE identified serine/threonine protein kinase 25 (STK25) as a candidate gene exhibiting abnormal m6A modification, and its expression was subsequently validated using reverse transcription-quantitative (RT-q)PCR. CD4+ T cells isolated from MRL/lupus-prone mice underwent lentiviral-mediated STK25 knockdown, and glycolytic activity was evaluated through measuring glucose uptake and lactate production. Western blotting and RT-qPCR demonstrated that STK25 knockdown reduced the expression of glycolysis-associated genes (glucose transporter 1, hexokinase 2, pyruvate kinase M2 and lactate dehydrogenase A) and the Treg-specific transcription factor Foxp3, while increasing the expression of the Th17- and Th2-associated transcription factors, RORγt and Gata3. Flow cytometry further confirmed enhanced differentiation of Th17 and Th2 cells accompanied by a reduction in Treg cells, indicating disruption of immune homeostasis. Collectively, these findings suggested that aberrant m6A modification contributes to the downregulation of STK25 expression, thereby promoting glycolytic reprogramming and CD4+ T-cell subset imbalance in SLE. STK25 may therefore represent a potential therapeutic target for restoring immune homeostasis in SLE.
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7. GSE264036 Pcbp1 由于调节碳水化合物和氨基酸代谢，对幼稚型多能性向启动型多能性转变至关重要 [ChIP-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、ChIP-seq
• 📝 描述：Contributors : Evgeny Bakhmet ; Evgeny Potapenko ; Nadezhda VorobyevaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusEmbryo implantation coincides with the Naïve-to-Primed pluripotency transition. From fertilization to implantation only a number of cleavages is observed without significant embryo growth. Embryo-uterine connection provides necessary nutrients for further development while metabolism intensification is occurred. We have found that in vitro Naïve-to-Primed pluripotency transition of ESCs with Pcbp1 knockout is worsened – reduction of proliferation and cell death is observed. By using ChIP-seq, RNA-seq and Mass-spectrometry we show that Pcbp1 is detrimental for pluripotent properties, but is crucial for carbohydrate and amino acid metabolism intensification. According to this, functional approaches showed protein synthesis, glycolysis and respiration decline, while embryos deficient for Pcbp1 demonstrated growth absence after implantation.
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8. GSE264035 Pcbp1 由于调节碳水化合物和氨基酸代谢，对幼稚型多能性向启动型多能性转变至关重要 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、RNA-seq
• 📝 描述：Contributors : Evgeny Bakhmet ; Evgeny PotapenkoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusEmbryo implantation coincides with the Naïve-to-Primed pluripotency transition. From fertilization to implantation only a number of cleavages is observed without significant embryo growth. Embryo-uterine connection provides necessary nutrients for further development while metabolism intensification is occurred. We have found that in vitro Naïve-to-Primed pluripotency transition of ESCs with Pcbp1 knockout is worsened – reduction of proliferation and cell death is observed. By using ChIP-seq, RNA-seq and Mass-spectrometry we show that Pcbp1 is detrimental for pluripotent properties, but is crucial for carbohydrate and amino acid metabolism intensification. According to this, functional approaches showed protein synthesis, glycolysis and respiration decline, while embryos deficient for Pcbp1 demonstrated growth absence after implantation.
• 🔗 查看原文

9. GSE333510 单核细胞中的基线干扰素信号传导和注射部位抗体介导的先天激活影响 mRNA 疫苗的反应原性 [NanoString nCounter 数据集]

• ✍️ 作者：未知作者
• 🏷️ 关键词：vaccine、antibody
• 📝 描述：Contributors : Natacha Madelon ; Gustavo A Ruiz Buendía ; Arnaud M DidierlaurentSeries Type : Expression profiling by arrayOrganism : Mus musculusThe local and systemic symptoms that follow vaccination -collectively referred to as reactogenicity- are common, yet the mechanisms underlying individual variability remain poorly understood. Through longitudinal immune profiling of vaccinated individuals and mechanistic studies in mice, we identified key immunological determinants of reactogenicity induced by mRNA vaccines. Systemic adverse events were associated with stronger interferon and pro-inflammatory responses following the second dose of COVID-19 vaccine, which were also correlated with the magnitude of the antigen-specific adaptive responses. This heightened inflammation occurred within 24 hours of vaccination and originated primarily from the injection site and was characterized by enhanced recruitment and activation of myeloid cells, particularly monocytes. Two mechanisms contributed to this response: (1) early interferon production by muscle T cells generated after the first dose and (2) FcγR-dependent chemokine induction by antigen-specific antibodies. Consistently, serum antibody levels prior to vaccination correlated positively with reactogenicity. In addition to this local amplification mechanism, variability in reactogenicity was influenced by the baseline immune state, as individuals with a pre-existing interferon-stimulated gene signature in monocytes, detectable at both transcriptomic and epigenetic levels, were more prone to systemic symptoms. Our findings reveal molecular and cellular mechanisms driving vaccine reactogenicity, providing a framework for the design of less reactogenic vaccines.
• 🔗 查看原文

10. GSE333235 单核细胞中的基线干扰素信号传导和注射部位抗体介导的先天激活影响 mRNA 疫苗的反应原性 [human_RNAseq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：vaccine、antibody
• 📝 描述：Contributors : Natacha Madelon ; Gustavo A Ruiz Buendía ; Arnaud M DidierlaurentSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe local and systemic symptoms that follow vaccination -collectively referred to as reactogenicity- are common, yet the mechanisms underlying individual variability remain poorly understood. Through longitudinal immune profiling of vaccinated individuals and mechanistic studies in mice, we identified key immunological determinants of reactogenicity induced by mRNA vaccines. Systemic adverse events were associated with stronger interferon and pro-inflammatory responses following the second dose of COVID-19 vaccine, which were also correlated with the magnitude of the antigen-specific adaptive responses. This heightened inflammation occurred within 24 hours of vaccination and originated primarily from the injection site and was characterized by enhanced recruitment and activation of myeloid cells, particularly monocytes. Two mechanisms contributed to this response: (1) early interferon production by muscle T cells generated after the first dose and (2) FcγR-dependent chemokine induction by antigen-specific antibodies. Consistently, serum antibody levels prior to vaccination correlated positively with reactogenicity. In addition to this local amplification mechanism, variability in reactogenicity was influenced by the baseline immune state, as individuals with a pre-existing interferon-stimulated gene signature in monocytes, detectable at both transcriptomic and epigenetic levels, were more prone to systemic symptoms. Our findings reveal molecular and cellular mechanisms driving vaccine reactogenicity, providing a framework for the design of less reactogenic vaccines.
• 🔗 查看原文

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1. 研究人员揭示了一种侵袭性脑瘤如何逃避治疗

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor
• 📝 描述：Integrated DNA and single-cell RNA sequencing reveals how recurrent IDH-mutant gliomas evolve into more aggressive, treatment-resistant tumors through genetic and cellular state changes… The post Researchers uncover how an aggressive brain tumor evades treatment appeared first on RNA-Seq Blog.
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2. 科学家终于攻克了一种“不可成药”的胰腺癌靶点，使患者的生存期几乎翻了一番。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：For decades, pancreatic cancer has been one of the most lethal cancers, with few effective treatment options. A new drug, daraxonrasib, targets the KRAS mutation that fuels most pancreatic tumors—something many scientists once thought couldn’t be done. In a major clinical trial, the treatment nearly doubled survival for patients with advanced disease and reduced the risk of death by 60%.
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• GSE327417 人类肠道相关淋巴组织图谱揭示了B细胞的免疫调节相互作用
• GSE306916 吞噬作用诱导肿瘤相关巨噬细胞的促血管生成功能和染色质重塑 [ATAC-seq]
• GSE226737 对造血细胞进行系统代谢组学分析，发现尿苷是造血过程中重要的抗衰老因子
• GSE333959 m6A 阅读器 IGF2BP3 通过修饰 circ-EPB41L2 促进子宫内膜癌进展
• GSE314172 齐黄健脾紫肾汤治疗系统性红斑狼疮小鼠模型的肾脏mRNA测序
• GSE303731 单细胞 RNA 测序数据来自多种小鼠组织和人类肢体肌肉的血管内皮细胞。
• GSE282492 分枝杆菌感染中不同库普弗细胞亚群的单细胞RNA测序
• GSE264037 Pcbp1 通过调控碳水化合物和氨基酸代谢，在原始多能性向启动多能性转变过程中起着至关重要的作用。
• GSE334447 蝰蛇周围神经系统的髓鞘发育
• GSE333724 RNA测序数据显示，嗜水弧菌（Vibrio natriegens）的固氮酶调控与其他γ-变形菌不同。
• GSE333743 ZEB1对淋巴内皮细胞中VE-钙黏蛋白信号传导的修饰
• GSE333534 在TRP53/KRAS小鼠和类器官模型中靶向KRAS可抑制巴雷特食管向胃食管腺癌的进展
• GSE328294 IBI351，一种新型 KRASG12C 抑制剂，可增强 αPD-1 在 KRASG12C 突变型肺癌中的疗效
• GSE326341 人类皮肤鳞状细胞癌异种移植小鼠模型的特征分析
• GSE300757 吞噬作用诱导肿瘤相关巨噬细胞的促血管生成功能和染色质重塑
• GSE299977 新生儿败血症中由 TLR4 和 TLR1/2 配体触发的代谢权衡导致神经呼吸回路的独特功能障碍
• GSE249357 谷氨酰胺对EMT和肺癌转移的影响
• GSE333230 转录因子IRF8驱动CD8 T细胞的肿瘤特异性耗竭
• GSE308583 全基因组范围内毛霉目lncRNA的发现揭示了其在EDF生物学中的关键作用