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1. ⭐ GSE328342 纳米技术实现的空间选择性蛋白质降解调节乳酸代谢，从而增强肝癌的抗肿瘤免疫力

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immunity、metabolism、spatially
• 📝 描述：Contributors : Jinhong Du ; Shu Han ; Rui Song ; Jianze Wang ; Linyu Zhang ; Yueyang Xu ; Haoyi Zhou ; Feng Wang ; Sen Qin ; Da Xu ; Yameng Hao ; Kui Li ; Xin Zhou ; Jiadong Wang ; Luyang Sun ; Zhi Yang ; Zhaofei LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDysregulated cancer metabolism, driven in part by excessive lactate export through monocarboxylate transporter 1 (MCT1) and 4 (MCT4), generates an acidic tumor microenvironment (TME) that suppresses antitumor immunity and impairs therapy. While direct MCT1 and/or MCT4 inhibitors show promise, clinical translation remains limited by systemic toxicity. In this study, we developed polymer-based lysosome-targeting chimeras (LYTACs) that selectively degrade CD147, the MCT1/4 chaperone, in liver cancer cells, reducing lactate efflux in cancer cells while sparing healthy cells, thereby reprogramming lactate metabolism within the TME. We further engineered polymer-based acid-responsive CD147-targeting LYTACs (PARTACs) to achieve controlled intratumoral release under acidic conditions. Systemic administration of PARTACs significantly suppressed tumor progression in several orthotopic liver cancer models. Moreover, PARTACs sensitized tumors to chemo-, immuno-, and radiotherapy with a favorable safety profile. Collectively, our nano-enabled spatially selective strategy modulates lactate metabolism in vivo, augmenting antitumor immunity and improving standard-of-care cancer therapies efficacy.
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2. ⭐ GSE330123：炎症性肠病II型（克罗恩病和溃疡性结肠炎）患者及健康对照者外周血单核细胞的单细胞转录组学和T细胞受体β链测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell、transcriptomics
• 📝 描述：Contributors : Joshua E Chan ; Azam Mohsin ; Jens Krijgsman ; Ciska Lindelauf ; Qinghui Mu ; Brianna Cavalla ; Xuhuai Ji ; Sarah E Streett ; Vincent van Unen ; Mark M DavisSeries Type : OtherOrganism : Homo sapiensWe profiled peripheral blood mononuclear cells (PBMCs) from patients with Crohn’s disease (CD), ulcerative colitis (UC), and healthy controls (HC) using single-cell RNA sequencing (BD Rhapsody Targeted Analysis, Parse Evercode WT v2) and bulk T-cell receptor beta-chain sequencing (Adaptive ImmunoSEQ hsTCRB v4). A subset of donors provided matched samples for both assays.
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3. ⭐ GSE330122 炎症性肠病（克罗恩病和溃疡性结肠炎）患者及健康对照者外周血单核细胞的单细胞转录组学和T细胞受体β链批量测序 I

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell、transcriptomics
• 📝 描述：Contributors : Joshua E Chan ; Azam Mohsin ; Jens Krijgsman ; Ciska Lindelauf ; Qinghui Mu ; Brianna Cavalla ; Xuhuai Ji ; Sarah E Streett ; Vincent van Unen ; Mark M DavisSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe profiled peripheral blood mononuclear cells (PBMCs) from patients with Crohn’s disease (CD), ulcerative colitis (UC), and healthy controls (HC) using single-cell RNA sequencing (BD Rhapsody Targeted Analysis, Parse Evercode WT v2) and bulk T-cell receptor beta-chain sequencing (Adaptive ImmunoSEQ hsTCRB v4). A subset of donors provided matched samples for both assays.
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4. GSE316572 半乳糖凝集素-1 (LGALS1) 敲除和过表达的人胃癌7901细胞系的转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、transcriptome
• 📝 描述：Contributor : Chao WangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensGalectin1 overexpression gastric cancer cell lines have a stronger ability of invasion and metastasis. We constructed cells with high and low expression of Galectin1, and wanted to see the changes of downstream pathways by transcriptome sequencing.
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5. GSE325935 脑转移瘤中的单核和空间转录组数据集 [Xenium]

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial
• 📝 描述：Contributors : Aaditya Khatri ; Vaibhav Jain ; Ann Marie Pendergast ; Carey K Anders ; Simon G Gregory ; Amanda Van SwearingenSeries Type : OtherOrganism : Homo sapiensWe performed single nuclei and spatial transcriptomic sequencing on a cohort of solid tumor human brain metastasis specimens resected at Duke University Medical Center from 1998 to 2022. This includes 23 single nuclei specimens, 12 Visium standard specimens, and 4 Xenium specimens. These samples encompass metastases from lung cancer, breast cancer, melanoma, and sarcoma primaries. Analysis of these data revealed novel tumor-microenvironment cell-cell interactions associated with patient survival.
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6. GSE325196 脑转移瘤的单核和空间转录组数据集

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial
• 📝 描述：Contributors : Aaditya Khatri ; Vaibhav Jain ; Ann Marie Pendergast ; Carey K Anders ; Simon G Gregory ; Amanda Van SwearingenSeries Type : Other ; Expression profiling by high throughput sequencingOrganism : Homo sapiensWe performed single nuclei and spatial transcriptomic sequencing on a cohort of solid tumor human brain metastasis specimens resected at Duke University Medical Center from 1998 to 2022. This includes 23 single nuclei specimens, 12 Visium standard specimens, and 4 Xenium specimens. These samples encompass metastases from lung cancer, breast cancer, melanoma, and sarcoma primaries. Analysis of these data revealed novel tumor-microenvironment cell-cell interactions associated with patient survival.
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7. GSE301060：过敏原包裹纳米颗粒处理的过敏小鼠的B细胞基因表达

• ✍️ 作者：未知作者
• 🏷️ 关键词：B cell
• 📝 描述：Contributors : Laila M Rad ; Michael N Saunders ; Jessica J O’Konek ; Lonnie D SheaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPolymeric allergen-loaded nanoparticles were used as a treatment for a murine model of food allergies. To study changes in B cell gene expression due nanoparticle treatment, bulk RNA sequencing of B cells from the peyer’s patches (PP) and spleen was done after treatment.
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8. GSE300948 过敏小鼠小肠在过敏原包裹纳米颗粒治疗后的免疫动力学

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune
• 📝 描述：Contributors : Laila M Rad ; Michael N Saunders ; Jessica J O’Konek ; Lonnie D SheaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPolymeric allergen-loaded nanoparticles were used as a treatment for a murine model of food allergies. To study the immune changes due to allergic disease and nanoparticle treatment, single-cell RNA sequencing of the small intestine lamina propria was done at various timepoints. This study investigates immune dynamics in allergic and allergic nanoparticle-treated mice before and after OFC-induced anaphylaxis.
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9. GSE331455 小鼠胚胎肢间质类器官在指状形态发生过程中的单细胞RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：scRNA
• 📝 描述：Contributors : Rio Tsutsumi ; Mototsugu EirakuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe performed single-cell RNA sequencing of mouse embryonic limb mesenchymal cells and limb mesenchymal organoids during digit-like morphogenesis. Distal and proximal limb mesenchymal cells were isolated from E11.5 Slc:ICR mouse embryos, labeled using lipid-DNA CellPlex/Feature Barcode oligonucleotides, and profiled either immediately as Day0 samples or after culture as limb mesenchymal organoids under serum-only control or Fgf8b/Wnt3a-supplemented conditions. The dataset includes Day0 distal and proximal cells, Day1 and Day2 control organoids, and Day1 and Day2 Fgf8b/Wnt3a-treated organoids. These data were used to characterize distal and proximal cell states, treatment-dependent transcriptional changes, and lineage/state trajectories associated with self-organized digit-like morphogenesis.
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10. GSE331442 空间信息学习和最优运输揭示了ALS中星形胶质细胞形态状态与转录变异的解耦

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatially
• 📝 描述：Contributors : Elisa Messori ; Doaa Taha ; Lisa Fournier ; Anna Foix Romero ; Virginie Uhlman ; Pascal Frossard ; Cédric Vincent-Cuaz ; Rickie Patani ; Raphaëlle LuisierSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAstrocyte reactivity is increasingly recognised as a central contributor to neurological disease, yet astrocyte states do not resolve into discrete categories and their morphological organisation remains poorly understood. While transcriptomic studies have revealed substantial molecular heterogeneity, scalable frameworks for reconstructing the topology and plasticity of astrocyte morphological states are lacking. Here, we generated a multimodal dataset of human iPSC-derived astrocytes from ALS patients carrying VCP mutations and matched controls, profiled by high-content fluorescence imaging and bulk RNA sequencing under basal and inflammatory conditions. We developed SI-SimCLR, a spatially informed self-supervised learning framework that learns biologically meaningful image representations without segmentation or manual annotation. Building on these representations, we introduce an optimal transport (OT)-based framework that reconstructs astrocyte phenotypic landscapes as interconnected morphological state manifolds, enabling inference of attractor-like, transient, and highly plastic intermediary substates. SI-SimCLR outperformed standard self-supervised and pretrained baselines in capturing disease- and inflammation-associated morphology across experimental batches. OT-based landscape analysis revealed that VCP-mutant astrocytes occupy a constrained genotype-specific region under basal conditions, while inflammatory stimulation selectively remodels these states through highly connected intermediary configurations toward control-associated morphologies. Substate-level analysis further uncovered heterogeneous cell-autonomous reactive programmes within untreated VCP-mutant astrocytes that differentially recapitulate inflammation-associated states. Integration with matched transcriptomic profiling revealed a striking dissociation between molecular and morphological organisation: inflammatory stimulation dominated transcriptional variation, whereas ALS-associated mutation was the principal driver of morphological state organisation. Together, these findings establish a general framework for topology-aware reconstruction of cellular phenotypic landscapes and demonstrate that morphology encodes disease-associa…
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1. 科学家发现衰老的隐藏驱动因素——一种简单的补充剂逆转了大脑衰退

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：A newly identified brain protein may play a major role in how the body ages. Researchers discovered that declining levels of Menin in the hypothalamus triggered inflammation, memory problems, bone loss, and other aging-related changes in mice. Restoring Menin reversed several of these effects, while a simple amino acid supplement called D-serine boosted cognition. The discovery opens a surprising new path for fighting age-related decline.
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• GSE331389 组蛋白变体MacroH2A1在K134位点的乳酸化调控骨髓间充质干细胞的成骨分化
• GSE331372 CYCLON 是一种核仁蛋白，它通过核糖体生物合成调节泛癌细胞的适应性。
• GSE319770 心脏结节病炎症和自身免疫信号的整合分子分析 [snRNA-Seq]
• GSE298339 中华绒螯蟹（Eriocheir sinensis）胚柄在六种温度条件下的参考基因组组装