详细内容（前10条）

1. ⭐ GSE320059 Nur77激动剂增强自然杀伤细胞对肝细胞癌的免疫力[空间转录组学]

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、immunity、spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Shi Yong Neo ; Nicholas Ang ; Menaka Rajapakse ; Kong-Peng LamSeries Type : OtherOrganism : Homo sapiens ; synthetic constructDespite promising development as emerging “off-the-shelf” therapeutics against cancer, natural killer (NK) cells still faced considerable challenges in the solid tumor microenvironment (TME) including poor penetrance and immuno-suppression. Here, we employed spatial and single-cell transcriptomics to reveal a role for Nur77 in NK cell-mediated immunity against hepatocellular carcinoma (HCC). The expression of NR4A1, encoding Nur77, is associated with NK cell proliferation, activation of the immunostimulatory AP-1 gene regulons and better disease-free survival in HCC. Conditional ablation of Nr4a1 in NK cells perturbed their homeostasis and accelerated tumor progression in multiple tumor models while the agonistic activation of Nur77 in NK cells ex-vivo or in-vivo enhanced their anti-tumor functions. Mechanistically, Nur77 activation attenuated CD36 expression in NK cells and conferred resistance against oxLDL-mediated immunosuppression in the TME. Collectively, our findings highlighted the potential of harnessing Nur77 agonism in improving NK cell-based immunotherapy against tumors.
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2. ⭐ GSE330930 放射性药物通过肿瘤增敏和 TME 重塑增强 CAR T 细胞对放射敏感和放射抗性神经母细胞瘤的疗效 [scRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、TME、scRNA
• 📝 描述：Contributors : Rosa Nguyen ; Robert Edinger ; Constanza Rodriguez ; Xiyuan ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensChimeric antigen receptor (CAR) T-cell therapy has limited efficacy against solid tumors such as neuroblastoma (NB). Key obstacles include extensive tumor burden and the presence of an immunosuppressive tumor microenvironment (TME). We employ targeted radiopharmaceutical therapy (RPT) using [67Cu]Cu-LLP2A and find that it potentiated the anti-tumor activity of CAR T-cells in radio-sensitive and radio-resistant NB models via distinct mechanisms. In radio-sensitive NB, RPT is directly tumoricidal while also enhancing CAR T-cell efficacy through pro-immune pathways, most notably via the TNFα pathway, leading to paracrine activation of T-cells. In radio-resistant NB, RPT improves CAR T-cells by remodeling the myeloid compartment in the TME and increasing the formation of immunological niches of cytotoxic CD8⁺ GZMB⁺ and CD4⁺ GZMB+ CAR T-cells. While neither treatment modality alone can effectively treat NB, the combination of VLA-4-targeted RPT and GD2 or B7-H3 CAR T-cells augments anti-tumor efficacy, resulting in marked tumor regression in preclinical NB models.
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3. ⭐ GSE330929 放射性药物通过肿瘤增敏和 TME 重塑增强 CAR T 细胞对放射敏感和放射抗性神经母细胞瘤的疗效 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、TME、RNA-seq
• 📝 描述：Contributors : Rosa Nguyen ; Robert Edinger ; Constanza Rodriguez ; Xiyuan ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensChimeric antigen receptor (CAR) T-cell therapy has limited efficacy against solid tumors such as neuroblastoma (NB). Key obstacles include extensive tumor burden and the presence of an immunosuppressive tumor microenvironment (TME). We employ targeted radiopharmaceutical therapy (RPT) using [67Cu]Cu-LLP2A and find that it potentiated the anti-tumor activity of CAR T-cells in radio-sensitive and radio-resistant NB models via distinct mechanisms. In radio-sensitive NB, RPT is directly tumoricidal while also enhancing CAR T-cell efficacy through pro-immune pathways, most notably via the TNFα pathway, leading to paracrine activation of T-cells. In radio-resistant NB, RPT improves CAR T-cells by remodeling the myeloid compartment in the TME and increasing the formation of immunological niches of cytotoxic CD8⁺ GZMB⁺ and CD4⁺ GZMB+ CAR T-cells. While neither treatment modality alone can effectively treat NB, the combination of VLA-4-targeted RPT and GD2 or B7-H3 CAR T-cells augments anti-tumor efficacy, resulting in marked tumor regression in preclinical NB models.
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4. ⭐ GSE306750 巨噬细胞衍生的胆固醇代谢物对肺部 2 型炎症的空间组织影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、inflammation、spatial
• 📝 描述：Contributors : Yufan Zheng ; Hannah E Dobson ; Makheni J Pierre ; Lilly LeBlanc ; Dominic P Golec ; Nathan Carrillo ; Anshu Deewan ; Claudia R Cifuentes ; Eduard Ansaldo ; Eric V DangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusEffective pulmonary immunity requires the precise spatial organization of immune cells, yet the mechanisms guiding their intratissue positioning during inflammation remain unclear. Here, we identify a cholesterol-derived chemotactic axis that spatially organizes T helper 2 (TH2) cells during fungal-induced pulmonary type 2 inflammation. Inflammation-expanded macrophages expressing cholesterol-25-hydroxylase (CH25H) produce 25-hydroxycholesterol, which is converted into the oxysterol 7α,25-dihydroxycholesterol to attract GPR183-expressing TH2 cells into inflammatory Ly6C+ macrophages, promoting fungal persistence. Disruption of this axis via TH2- specific GPR183 deletion restores type 1 macrophage activation and enhances fungal clearance. Our findings reveal a macrophage-driven, metabolite-based mechanism of immunosuppressive cell positioning in inflamed lung tissue.
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5. ⭐ GSE326288 早产会改变出生后48小时内髓系来源抑制细胞的发育轨迹：单细胞转录组学和推断的细胞间通讯

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、transcriptomics、trajectory
• 📝 描述：Contributors : Miguel Hernández-Ríos ; Jaimar Rincon ; Leandro Balzano-Nogueira ; Valerie Polcz ; Dayuan Wang ; Whitman Wiggins ; Christine Rodhouse ; Athina Yoham ; Feifei Xiao ; Ricardo Ungaro ; Marvin Dirain ; Lyle Moldawer ; Robert Maile ; Guoshaui Cai ; Philip Efron ; Shawn LarsonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBackground: Prematurity is a leading cause of neonatal and childhood mortality, with infections driving early deaths. Innate immunity provides frontline defense after birth and is shaped in part by myeloid-derived suppressor cells (MDSCs). The role of MDSCs in regulation of neonatal immunity, especially in the context of prematurity, remains elusive. We sought to understand the transcriptional landscape of neonatal immune myeloid regulators, specifically differences between preterm and full-term neonates. Insight into specific cellular networks could help understand how to skew preterm MDSCs’ development towards classical immunotolerant and anti-microbial mechanisms. Methods: This cross-sectional study used single-cell RNA sequencing to characterize the neonatal MDSC transcriptional landscape, developmental trajectories, and predicted signaling networks within 48 hours after birth. Peripheral blood mononuclear cells were isolated from 7 preterm neonates (37 weeks), and 6 healthy adult (21-45 years old, control). Primary exposure was premature birth and neonatal intensive care hospitalization from a single-center, academic tertiary care hospital between 2023 – 2024. Results: Among ~339,000 cells, preterm neonates exhibited enrichment of polymorphonuclear MDSCs (10.6%±5.3%) vs full-term (2.6%±1.3%) and adults (0.4%±1.3%). Trajectory analysis identified a prematurity-associated differentiation branch characterized by inflammatory signaling, mitochondrial stress, and heightened protein-translation programs, distinct from a conserved, tolerogenic MDSC trajectory present across ages. Cell-communication modeling showed intensified outgoing and incoming signaling via ADGRE, RESISTIN, TGF-β, ANNEXIN, and ICAM networks. Antigen presentation signatures suggested preserved MHC-I output and diminished MHC-II interactions in neonates, with increased MHC-I input to preterm polymorphonuclear MDSCs. Conclusions: Prematurity is associated with early divergence of MDSC maturation toward an inflammatory and metabolically stressed PMN-MDSC state with altered immune communication. These findings identify cellula…
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6. ⭐ GSE314743 Nur77激动剂增强自然杀伤细胞对肝细胞癌的免疫力[scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、immunity、scRNA
• 📝 描述：Contributors : Shi Yong Neo ; Nicholas Ang ; Kong-Peng LamSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSingle-cell liver transcriptomic profiling of mice with conditional ablation of Nr4a1 in NK cells
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7. ⭐ GSE312294 Nur77激动剂增强自然杀伤细胞对肝细胞癌的免疫力[ChIP-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、immunity、ChIP-seq
• 📝 描述：Contributors : Shi Yong Neo ; Menaka P RajapakseSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensDNA binding motifs of Nur77 in NK cells
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8. GSE331196 对感染新型隐球菌gcs1突变体的混合骨髓嵌合小鼠肺髓系细胞进行单细胞RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Contributors : Yufan Zheng ; Eduard Ansaldo ; Claudia A Rivera ; Eric V DangSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusscRNA-seq of sorted pulmonary myeloid cells (Live/CD45+/IV-/B220-/CD90.2-/Ly-6G-/MHCII+/CD11c+/CD64+) from mixed bone marrow chimeric mice at 35 days post-infection with C. neoformans gcs1 mutant. Chimeric mice were reconstituted with CD45.1 WT : CD45.2 WT (WT chimeras) or CD45.1 WT : CD45.2 STAT6-KO (STAT6-KO chimeras). Cells were labeled with hashtag antibodies (TotalSeq-C, BioLegend) and pooled for library preparation. No sample was excluded due to quality issues.
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9. GSE330931 放射性药物通过肿瘤增敏和肿瘤微环境重塑增强CAR T细胞对放射敏感和放射抗性神经母细胞瘤的疗效

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、TME
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
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10. GSE328772 Jagged-1+ Tregs介导肿瘤引流淋巴结的淋巴管重塑

• ✍️ 作者：未知作者
• 🏷️ 关键词：lymph、regex:lymph(o|atic)?
• 📝 描述：Contributors : Jessie Z Xu ; Inchul Cho ; Niwa AliSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo investigate the role of Jag1⁺ Tregs in regulating the activity of tumours and corresponding tdLNs in the B16-F10 tumour model, we performed bulk RNA sequencing on tumours and tdLNs of wild-type mice and mice with conditional deletion of Jag1 in Tregs. Transcriptomic analysis revealed no significant changes in gene expression in tumours, whereas tdLNs exhibited downregulation of lymphatic endothelial cell (LEC)-related markers and genes related to cell junction organization in mice with Treg-specific Jag1 ablation.
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详细内容（全部2条）

1. 了解高危膀胱癌的治疗反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：RNA sequencing and multi-omics profiling identified four molecular subtypes of high-risk bladder cancer, helping explain differences in immunotherapy response and recurrence risk…
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2. 人体细胞上隐藏的糖类模式可能有助于早期发现癌症。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have uncovered a hidden “sugar code” on the surface of human cells that could transform how diseases are detected. Using an advanced imaging technique called Glycan Atlasing, researchers at the Max Planck Institute mapped the tiny sugar structures coating cells and discovered that these patterns shift depending on what the cell is doing. Immune cells changed their sugar layouts when activated, and cancerous tissues displayed distinct surface signatures compared to healthy tissue.
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• GSE320244 雷公藤内酯通过靶向 XIAP 诱导宿主细胞凋亡，同时保持肠道菌群稳态，从而清除金黄色葡萄球菌感染 [小鼠肺巨噬细胞]
• GSE320243 雷公藤内酯通过靶向 XIAP 诱导宿主细胞凋亡来清除金黄色葡萄球菌感染，同时保持肠道菌群稳态 [iBMDMs]
• GSE315654 整合多组学分析揭示杂交水稻通过重编程碳代谢获得抗旱性
• GSE325899 暴露于BeO的CCL3/4缺陷型HLA-DP2转基因小鼠或接受新型HLA-DP2/CCL-CAR-T细胞治疗的小鼠肺部趋化因子特异性CD4+T细胞积累减少
• GSE302178 出生时脐带血细胞转录和甲基化特征与支气管肺发育不良 (BPD) 的发生相关 [RNA-Seq]
• GSE286264 CCR1驱动胰腺癌肿瘤相关巨噬细胞的免疫抑制特性
• GSE330222 短读长和长读长单细胞测序捕捉到 CRISPR 筛选中不同的扰动效应
• GSE328291 解析 METTL5 特异性：直接 RNA 测序揭示 mESC 中不存在 METTL5 介导的 mRNA m6A 甲基化
• GSE331195 E11.5 主动脉-性腺-中肾区 CD31 内皮细胞的 CUT&RUN 测序
• GSE331194 CD4+ Tregs 通过内皮 YY1/MAML1 再激活驱动缺血后萌芽血管生成 [ChIP-seq]
• GSE331193 CD4+ Tregs 通过内皮 YY1/MAML1 再激活驱动缺血后萌芽血管生成 [RNA-seq]
• GSE331185 夜间人工光照重塑了达氏雀鲷（Dascyllus aruanus damselfish）的昼夜脑转录组学
• GSE329393 RBM20 变异体在扩张型和非致密型心肌病患者特异性干细胞模型中诱导不同的钙处理和代谢表型
• GSE325396 血浆和细胞外囊泡小RNA测序——重度创伤性脑损伤患者、多发性创伤患者和健康对照组
• GSE305415 软骨细胞脂质代谢的控制对骨骼生长至关重要
• GSE304221 果蝇神经索发育图谱揭示神经元身份的全局时间编码
• GSE303333 异常的 STAT 信号传导驱动可靶向治疗的儿童脓毒症表型中的 T 细胞失调
• GSE331137 甲基化 Mesa 定义了靶向基因激活的功能性调控元件
• GSE329002 从头嘧啶合成控制生发中心B细胞和浆细胞的命运以及系统性自身免疫
• GSE319702 由于 wbbL 突变导致的 O 抗原丢失很常见，并且与大肠杆菌血流感染死亡率增加相关
• GSE317482 单核 RNA 测序揭示牛乳腺细胞类型对热应激的特异性反应
• GSE317065 粪双歧杆菌 KC84 通过调节血清素相关通路和树突状细胞介导的 IFN-β 诱导来减轻 IBS-D 样症状
• GSE302179 出生时脐带血细胞转录和甲基化特征与 BPD 发育相关 [RRBS]
• GSE260896 MYB相关转录因子MYPOP作为癌细胞生长的选择性调节因子
• GSE331104 单碳循环和甲基化潜力通过谷氨酸能神经元调节酒精行为反应
• GSE331053 SRSF3 通过促进肝细胞表达 ERα 和 FOXA，在肝癌性别差异中发挥关键作用。
• GSE330985 胸腺中 abT 细胞（OT-I 和 OT-II）的阳性和阴性选择 2 - scRNA-seq、CITE-seq 和 TCRab
• GSE302373 伤害性神经支配限制三级淋巴结构以促进肺癌
• GSE297644 阿托伐他汀以孕烯醇酮16α-腈依赖的方式调节肝脏转录组，但其作用机制与孕烯醇酮16α-腈不同，且不诱导PXR介导的肝脂肪变性。