详细内容（前10条）

1. ⭐ GSE326061 Fc 优化的 GITR 抗体增强 CD4 T 细胞与树突状细胞的相互作用，从而促进抗肿瘤免疫 [Lib1]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、T cell、dendritic cell、antibody
• 📝 描述：Contributors : Yahel Avraham ; Natasha Barth ; Tzlil Yair Bar-on ; Barak Toval ; Aviya Habshush Menachem ; Jasmine Blanga ; Ella Herzog ; Hagar Rotem ; Yuval Shapir Itai ; Tali Feferman ; Moshe Biton ; Rony DahanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTargeting the stimulatory immune checkpoint glucocorticoid-induced TNFR-related protein (GITR) using agonistic monoclonal antibodies (mAbs) is a promising strategy for cancer immunotherapy that involves increased effector T cell activity and regulatory T cell (Treg) elimination. The pre-clinical anti-tumor activity of GITR mAbs depends on activating Fcγ receptors (FcγRs). However, the role of human Fc-FcγR interactions in the activity of GITR mAbs has not been comprehensively addressed. To this end, we employed Fc protein and glycan engineering to modify the FcγR interactions of anti-GITR human mAbs and characterized them in humanized FcγR mice. We identified an Fc-optimized human IgG scaffold that increased binding to activating FcγRIIa and FcγRIIIa, enhancing anti-tumor efficacy. This Fc-optimized activity was mediated by multiple mechanisms that are unique to GITR mAb, including FcγR-mediated Treg depletion and mutual engagement and activation of CD4+ T cells and dendritic cells, leading to anti-tumor cytotoxic activity of CD4+ T cells and enhanced CD8+ T cell activity. Our findings suggest a strategy to optimize human GITR mAbs, harnessing beneficial immune pathways to improve their therapeutic potential.
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2. ⭐ GSE325953 Fc 优化的 GITR 抗体增强 CD4 T 细胞与树突状细胞的相互作用，从而促进抗肿瘤免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、T cell、dendritic cell、antibody
• 📝 描述：Contributors : Yahel Avraham ; Natasha Barth ; Tzlil Yair Bar-on ; Barak Toval ; Aviya Habshush Menachem ; Jasmine Blanga ; Ella Herzog ; Hagar Rotem ; Yuval Shapir Itai ; Tali Feferman ; Moshe Biton ; Rony DahanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTargeting the stimulatory immune checkpoint glucocorticoid-induced TNFR-related protein (GITR) using agonistic monoclonal antibodies (mAbs) is a promising strategy for cancer immunotherapy that involves increased effector T cell activity and regulatory T cell (Treg) elimination. The pre-clinical anti-tumor activity of GITR mAbs depends on activating Fcγ receptors (FcγRs). However, the role of human Fc-FcγR interactions in the activity of GITR mAbs has not been comprehensively addressed. To this end, we employed Fc protein and glycan engineering to modify the FcγR interactions of anti-GITR human mAbs and characterized them in humanized FcγR mice. We identified an Fc-optimized human IgG scaffold that increased binding to activating FcγRIIa and FcγRIIIa, enhancing anti-tumor efficacy. This Fc-optimized activity was mediated by multiple mechanisms that are unique to GITR mAb, including FcγR-mediated Treg depletion and mutual engagement and activation of CD4+ T cells and dendritic cells, leading to anti-tumor cytotoxic activity of CD4+ T cells and enhanced CD8+ T cell activity. Our findings suggest a strategy to optimize human GITR mAbs, harnessing beneficial immune pathways to improve their therapeutic potential.
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3. ⭐ GSE330363 应激通过肠道微生物-代谢物轴加速肝细胞癌进展

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、regex:micro(b|be|bial|organism)、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Zhe Hu ; Pengfei Yue ; Minlan Yuan ; Zhenjie Zhang ; Biao Yang ; Zhongwei CaoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHepatocellular carcinoma is one of the most prevalent malignancies worldwide, and the role of stress in hepatocellular carcinoma progression remains incompletely understood. In this study, we integrated clinical and preclinical models to investigate how stress-associated gut microbiota remodeling contributes to hepatocellular carcinoma progression. Stress profoundly altered the gut microbiota, with Phocaeicola vulgatus significantly reduced. Restoration of Phocaeicola vulgatus or administration of its tryptophan-derived metabolite indole-3-propionic acid attenuated hepatocellular carcinoma progression in vivo. Single-cell RNA sequencing was performed to characterize changes in the hepatocellular carcinoma tumor microenvironment. Indole-3-propionic acid treatment reduced endothelial JAM2 expression and was associated with reduced JAM2-F11R-mediated endothelial-macrophage crosstalk. These findings support a role for the stress-gut microbiota-metabolite-tumor microenvironment axis in hepatocellular carcinoma progression and suggest potential translational targets for microbiome-based therapeutic strategies.
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4. ⭐ GSE330449 重编程细菌能量学逆转乳酸介导的免疫抑制以实现协同癌症免疫疗法

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、regex:bacter(ia|ial|ium)、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Jing Zhao ; Nuo Zhang ; Lingfeng Qin ; Wei Wei ; Xiuxiu Wang ; Jingjing Liu ; Jing WangSeries Type : Expression profiling by high throughput sequencingOrganism : Shewanella oneidensis MR-1The immunosuppressive tumor microenvironment (TME), driven by lactate accumulation, critically limits cancer immunotherapy efficacy. Here, we engineered Shewanella oneidensis MR-1 (S. oneidensis) by reprogramming energy metabolism to reverse lactate-driven immunosuppression in the TME. Integration of polyphosphate kinase 2 (PPK2-Ⅰ) and NAD kinase (NADK) markedly augmented bacterial bioenergetics, boosting ATP production by 287.5% and elevating the NADH/NAD⁺ ratio by 299.9% compared to the wild-type strain. This bioenergetic enhancement accelerated targeted lactate depletion and increased intratumoral bacterial persistence, while impairing tumor lactate transport via downregulation of monocarboxylate transporters MCT1 and MCT4. Crucially, metabolic remodeling reversed immunosuppression by enhancing antigen presentation and CD8+ T cell infiltration, and reducing regulatory T cells (Tregs), thereby converting immunologically “cold” tumors into immunoreactive phenotypes. When combined with αPD-1 immunotherapy, this strategy synergistically amplified antitumor efficacy and established durable immunological memory against recurrence. Collectively, our work establishes a paradigm for bacterial bioenergetic engineering to advance cancer immunotherapy.
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5. GSE330188 Crnic研究所人类三体计划 - 托法替尼治疗唐氏综合征免疫性皮肤病：全血PolyA RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、RNAseq
• 📝 描述：Contributors : Matthew D Galbraith ; Angela L Rachubinski ; Joaquin M EspinosaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAnalysis of steady-state mRNA levels in whole blood of subjects with Down syndrome (trisomy 21) and qualifying moderate-to-severe immune skin conditions. This dataset is part of the Human Trisome Project run by the Linda Crnic Institute for Down Syndrome at University of Colorado Anschutz. http://www.trisome.org/
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6. GSE312183 人类正常肝脏和肝细胞癌组织转录组分析，按 EZH2 表达水平分层

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、transcriptome
• 📝 描述：Contributor : Kyung Hyun YooSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis RNA-seq dataset was generated to investigate transcriptomic alteriations associated with hepatocellular carcinoma(HCC) progression and the functional role of EZH2 expression. Liver tissue samples were collected from normal controls, HCC patients with low EZH2 expression, and HCC patients with high EZH2 expression. Differential gene expression analysis confirmed a marked increase in EZH2 expression in HCC relative to normal tissues and revealed transcriptional programs associated with proliferative and metastatic characterisrics that were progressively enhanced according to EZH2 expression level. This dataset enables the exploration of EZH2-associated molecular signatures and their relevance to HCC malignancy and metastatic progression.
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7. GSE295456 慢性淋巴细胞白血病伴IGH::BCL3易位的特征是具有同质且独特的遗传和表观遗传图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、epigenetic
• 📝 描述：Contributors : Cosima Drewes ; Reiner SiebertSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensApproximately 1% of chronic lymphocytic leukemia (CLL) cases harbor a translocation juxtaposing the immunoglobulin heavy chain (IGH) and B-cell lymphoma 3 (BCL3) loci. Aiming at comprehensive molecular characterization of IGH::BCL3-positive B-cell neoplasms we here investigated samples from 84 patients using FISH, whole genome and targeted sequencing and DNA methylation analyses. Junctional sequences obtained in 27 patients showed breakpoints upstream of BCL3 in all CLL cases. IGH breaks were presumably driven by aberrant class-switch recombination in 26/27 cases, frequently involving IGHA (12/26). Notably, 95% (78/82) of patients carried an unmutated IGHV with significant CLL stereotype subset #8 enrichment. Trisomy 12 (61%, 51/83) and mutations affecting NOTCH1 (32%, 25/79), BRAF (14%, 11/79) and FBXW7 (14%, 11/79) were frequent aberrations. DNA methylation analysis assigned 77% (51/66) of patients with IGH::BCL3-translocation with at least 60% tumor cell content to the naive B-cell-like group but unraveled a distinct and during follow-up stable signature resembling in part plasma cell-like epigenetic features. A binary DNA methylation classifier using 20 CpGs could distinguish IGH::BCL3-translocated CLL samples from other CLL subtypes. In an efficacy cohort of 3832 previously untreated patients from GCLLSG trials, IGH::BCL3 was associated with shorter progression-free survival (PFS) and overall survival (OS) in 28 patients when treated with chemoimmunotherapy, but not in those receiving venetoclax. Our findings highlight the genetic and epigenetic homogeneity of IGH::BCL3-translocated CLL samples and their differences from other types of CLL suggesting IGH::BCL3 leukemic B-cell neoplasms to be a biological distinct subtype of CLL.
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8. GSE330702 RNA-seq 分析 TGF-β2 刺激下人真皮淋巴内皮细胞中 Nogo-B 敲低的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:lymph(o|atic)?、RNA-seq
• 📝 描述：Contributors : Dan Shan ; Yifeng Zhang ; Jun YuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensNogo-B (RTN4B), an endoplasmic reticulum-associated protein, plays a critical role in regulating endothelial cell function and vascular remodeling. However, its role in lymphatic endothelial cells (LECs) and in response to profibrotic signaling remains poorly understood. Transforming growth factor-β2 (TGF-β2) is a key regulator of endothelial phenotypes and has been implicated in lymphatic dysfunction under pathological conditions. In this study, we performed RNA sequencing (RNA-seq) to investigate the transcriptional changes associated with Nogo-B knockdown in human dermal lymphatic endothelial cells (HDLECs) under TGF-β2 stimulation. HDLECs were transduced with shRNA targeting Nogo-B (shNogo-B) or control shRNA (shGFP), followed by treatment with TGF-β2 for 72 hours. Total RNA was extracted and subjected to high-throughput sequencing. Comparative transcriptomic analysis was conducted to identify differentially expressed genes and pathways regulated by Nogo-B under TGF-β2-stimulated conditions. This dataset provides insights into the molecular mechanisms by which Nogo-B modulates lymphatic endothelial cell responses to TGF-β signaling, including pathways related to extracellular matrix organization, cell migration, proliferation, and cytoskeletal remodeling. These data serve as a resource for understanding the role of Nogo-B in lymphatic endothelial biology and its contribution to TGF-β-mediated signaling and dysfunction.
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9. GSE330527 蛹翼蝠唾液腺全长转录组测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、transcriptome
• 📝 描述：Contributors : Chun He ; Jiamin ShiSeries Type : Expression profiling by high throughput sequencingOrganism : Pteromalus puparumAnimal saliva provides an excellent model for studying adaptive evolution, yet the functional significance of alternative mRNA isoforms in parasitoid salivary systems remains poorly understood. Here, using integrative full-length transcript sequencing and expression profiling, we generated an isoform-resolved transcriptomic landscape of salivary genes in the endoparasitoid wasp Pteromalus puparum. We identified 133 high-confidence salivary genes, more than 75% of which produced multiple transcript isoforms. Proteomic analyses confirmed that alternative splicing directly contributes to salivary protein diversity, with eight genes encoding distinct protein isoforms. Twelve salivary genes exhibited gland-biased isoform usage, including PpSerpin3, whose short isoform was specifically expressed in salivary and venom glands and suppressed host melanization. Comparative multi-omics analyses further revealed that salivary and venom systems share a conserved genetic core but achieve functional specialization through tissue-specific gene family co-option and extensive isoform switching. Together, these findings establish an isoform-resolved framework for the parasitoid salivary system and demonstrate that alternative splicing promotes salivary protein diversification and gland-specific functional evolution.
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10. GSE330392 通过双重TGF-β1给药途径建立和表征淋巴结纤维化小鼠模型

• ✍️ 作者：未知作者
• 🏷️ 关键词：lymph、regex:lymph(o|atic)?
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis study establishes two reliable TGF-β1–induced lymph node fibrosis models that recapitulate ECM remodeling and immune suppression, providing a platform to investigate how fibrotic changes disrupt local immune regulation.
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1. 新药有望清除与癌症和衰老相关的“僵尸细胞”。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、aging
• 📝 描述：Researchers found a new way to kill harmful “zombie” cells that linger after chemotherapy and help cancers become more aggressive. These senescent cells survive by relying on a protective protein called GPX4, even while sitting on the edge of a deadly iron-triggered collapse. New drugs remove that protection, causing the cells to self-destruct. In mice, the approach reduced tumor size and boosted survival, hinting at a promising new cancer therapy.
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2. 利用RNAP-seq绘制细菌转录图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:bacter(ia|ial|ium)
• 📝 描述：RNA sequencing method RNAP-seq maps bacterial transcription with single-nucleotide precision, revealing lineage-specific RNA polymerase pausing and regulatory factor…
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3. 一种罕见的抗癌植物化合物已被破译

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists at UBC Okanagan have uncovered how plants produce mitraphylline, a rare natural compound with promising anti cancer potential. The team identified two enzymes that work together to build the molecule’s unusual twisted structure, solving a mystery that had puzzled researchers for years. Because mitraphylline appears only in tiny amounts in tropical plants like kratom and cat’s claw, the discovery could make it far easier to produce sustainably in the future.
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4. 科学家在抗衰老领域取得重大突破，使衰老的血液干细胞重返青春。

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：As blood stem cells age, their lysosomes become overactive and damaged, triggering inflammation and weakening the body’s ability to regenerate healthy blood and immune cells. By calming this cellular “overdrive,” researchers restored the stem cells’ youthful function, dramatically boosting their ability to regenerate and produce balanced blood cells.
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• GSE298831 NSD1 控制 H3K36me2 介导的 DNA 甲基化，并通过调节 lncRNA 表达驱动人类 iPSC 分化 [ChIP-seq]
• GSE298830 NSD1 控制 H3K36me2 介导的 DNA 甲基化，并通过调控 lncRNA 表达驱动人类 iPSC 分化 [RNA-seq]
• GSE297059 全氟和多氟烷基物质 (PFAS) 抑制巨噬细胞的替代激活，从而破坏肝脏脂质代谢
• GSE330674 小鼠慢性社会应激期间的糖皮质激素抵抗：前额叶皮层的RNA测序
• GSE330673 欧洲枝孢霉在多氯联苯同系物存在下的 RNA 测序
• GSE330640 多顺反子基因座编码一种抑癌microRNA和一种调控果蝇睾丸形态的长链非编码RNA
• GSE330241 有丝分裂后神经元中 H3K27me3 的长期维持对于基因表达调控并非必需 [ChIP-Seq]
• GSE330143 有丝分裂后神经元中 H3K27me3 的长期维持对于基因表达调控并非必需 [ATAC-seq]
• GSE304460 H2B.8 的复制非依赖性驱逐揭示了种子吸胀过程中的大规模染色质重编程 [Hi-C]
• GSE293304 动脉-静脉分化过程中内皮细胞染色质可用性和转录因子活性的调控 - HUVEC FUCCI RNA测序
• GSE328315 E11.5 小鼠心脏 RNA 测序中心肌细胞 ATF4 CDS 或 pas 缺失
• GSE324984 MYC家族转换：L-MYC驱动并维持前列腺癌中的神经内分泌谱系程序
• GSE320141 MERVL 阳性胚胎干细胞的稳定维持揭示了持续的转录程序和增强子重塑 [scRNA-seq]
• GSE319519 通过 AAV-Lmna/DJ8 介导的心肌细胞特异性层粘蛋白 A 重表达可改善 Lmna⁻/⁻ 小鼠的心脏功能障碍
• GSE318500 RNA-seq 分析用 EPICERTIN 或霍乱毒素 B 亚单位处理的人类 PBMC 衍生巨噬细胞的转录反应。
• GSE314247：用于 Polycomb 染色质分析的 ChIP-seq、CUT&RUN 和 CUT&Tag 的比较分析
• GSE296887 一种新型治疗剂：利用巨噬细胞极化对抗脓毒症并减轻肺损伤
• GSE295507 RNA-seq 分析揭示了不同古菌物种中动态的 tRNA 修饰图谱
• GSE283388 塞利尼索治疗弥漫性大B细胞淋巴瘤（DLBCL）细胞系的基因表达谱分析
• GSE330551 Th2启动的先天免疫扩增定义了一种独特的特应性皮炎内型
• GSE330517 对野生型和 Lcn10 敲除小鼠的骨髓来源巨噬细胞 (BMDM) 进行 RNA 测序分析
• GSE330455 系统分析揭示了结核分枝杆菌在不同物种中采用的替代代谢状态
• GSE330430 FAP-CD3 T 细胞衔接器诱导混合 CAF 细胞群的出现限制了治疗效果
• GSE330353 人类心脏分化的动态调控结构
• GSE330338 多组学整合鉴定小细胞肺癌中的关键分子驱动因素和可变剪接事件
• GSE330336 通过核移植和特定因子诱导的人类多能干细胞的多组学分析（小RNA测序）
• GSE330335 通过核移植和特定因子衍生的人类多能干细胞的多组学分析（RNA-seq）
• GSE330333 通过核移植和特定因子诱导的人类多能干细胞的多组学分析（H3K27ac ChIP-seq）
• GSE330139 26 hpf 母系合子 MZ osgep G177A 纯合子混合样本的批量 RNA 测序
• GSE330110 纳米塑料暴露诱导肾小管上皮细胞线粒体功能障碍驱动的炎症和衰老
• GSE302357 冠状病毒在蝙蝠和人类细胞中的蛋白质相互作用图谱揭示了免疫逃避和复制的分子和遗传开关
• GSE298829 NSD1 控制 H3K36me2 介导的 DNA 甲基化，并通过调节 lncRNA 表达驱动人类 iPSC 分化 [甲基化芯片]
• GSE298146 SOX4 介导的母胎界面特定 T 淋巴细胞亚群分化导致早产 [RNA-Seq]
• GSE325448 了解内体/溶酶体失活后 HeLa 细胞的转录组变化
• GSE325258 HPV阳性宫颈癌细胞中残留的p53具有功能获得性特征
• GSE285966 恩格列净可恢复肥胖糖尿病HFpEF小鼠的心脏功能，但会进一步改变基因表达