详细内容（前10条）

1. ⭐ GSE330095 重塑胶质瘤中免疫抑制性肿瘤微环境可增强免疫介导基因治疗的疗效

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immune、tumor microenvironment、glioma
• 📝 描述：Contributors : Ziwen Zhu ; Pedro R Lowenstein ; Maria G CastroSeries Type : Methylation profiling by high throughput sequencingOrganism : Mus musculusGliomas account for ~80% of primary malignant brain tumors. Many CNS WHO grade 2–3 and some grade 4 gliomas harbor mutant isocitrate dehydrogenase 1 (mIDH1), which causes a gain of function mutation (IDH1 R132H) leading to the production of 2-hydroxyglutarate (2HG). Mutant IDH1-induced 2HG, through epigenetic reprogramming elicits an immune-permissive tumor microenvironment (TME). An immunosuppressive mechanism in the glioma TME involves adenosine production via the ectoenzyme CD73. This study investigates mIDH1’s influence on CD73 expression and adenosine levels. We demonstrate that mIDH1 glioma cells exhibit reduced CD73 expression, driven by DNA hypermethylation, leading to reduced adenosine levels.
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2. ⭐ GSE312327 异质性人类肿瘤组织的空间引导代谢组学分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、metabolomics、spatially
• 📝 描述：Contributors : Jia-Ying Joey Lee ; Jingtao Zhang ; Sin-Chi Chew ; Shihleone Loong ; Xu Liang ; Jia-Wen Carmen Kong ; Fedor Grigoryev ; Alexander Yaw-Fui Chung ; Jai-Yao Tao ; Peng-Chung Cheow ; Glenn Bonney ; Brian K.P. Goh ; Wei-Qiang Leow ; Yulan Wang ; Lit-Hsin Loo ; Pierce Kah-Hoe ChowSeries Type : OtherOrganism : Homo sapiensBulk high-resolution mass spectrometry can provide sensitive and global snapshots of metabolites involved in cancer metabolism. However, intra-tumor heterogeneity (IntraTH) convolutes the cellular origins and tumor pathologies associated with the detected metabolites, thus making the elucidation of reproducible metabolic pathways and/or biomarkers very challenging. Here, we present “Spatially-guided MEtabolomics (SgME) profiling”, a multi-modal metabolomics data analysis approach to delineate IntraTH by integrating spatial and bulk metabolomics profiles from the same tumors. We applied SgME profiling to 117 tumor and adjacent normal tissues from 26 surgically resected primary liver tumors, and constructed SgME maps of metabolic regions (MERs) associated with key histopathological features. We used these maps to survey IntraTH and train regression models that accurately predict the MER compositions of bulk tumor samples. We also discovered a group of putative metabolites that increase in low-grade tumor regions but abruptly decrease in necrotic regions. SgME profiling may also be applied to heterogeneous tissues from other cancer types or metabolic diseases and provide systems-level understandings of the roles of local cellular niches in cancer metabolism and tumorigenesis.
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3. ⭐ GSE307062 共生体靶向肽增强固氮作用而无需终末分化 [细菌 RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：bacteria、regex:bacter(ia|ial|ium)、RNA-seq
• 📝 描述：Contributor : Kevn D OliphantSeries Type : Expression profiling by high throughput sequencingOrganism : Mesorhizobium robiniaeLegumes have evolved lineage-specific peptides to control rhizobia and sustain nitrogen fixation. In inverted repeat-lacking clade (IRLC) legumes, nodule-specific cysteine-rich (NCR) peptides enforce terminal bacteroid differentiation, maximizing symbiotic output but restricting nodule lifespan. By contrast, Robinioid legumes, the IRLC’s sister clade, lack NCRs yet yet include perennial species that maintain productive nodules for decades. Here we identify a nodule-proline-glycine-rich peptide family (NPGs) as a conserved, genus-wide innovation of Robinia. NPGs are highly abundant, localize to the symbiosome membrane, and are intrinsically disordered. Exposure of Mesorhizobium robiniae to recombinant NPGs reprograms physiology, inducing nitrogenase expression while dampening growth without loss of viability. NPGs thus exemplify a distinct, reversible strategy of symbiont control, expanding the peptide repertoire that supports nitrogen fixation in perennial legumes.
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4. ⭐ GSE277030 UCA 重塑肿瘤微环境（scRNA-Seq）

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、tumor microenvironment、scRNA
• 📝 描述：Contributors : Yunlong Shan ; Mengying ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusUCA remodeled tumor microenvironment through unknown features and mechanisms. Single-cell RNA-sequencing of mouse MC38 model treated with UCA was designed to depict a comprehensive cellular landscape and potential mechanisms.
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5. GSE329994 单细胞RNA测序揭示海胆变态过程中的细胞状态

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Heliocidaris crassispinaMetamorphosis is a key process in the life history of most sea urchins. However, the understanding of its molecular mechanisms is still lacking, especially the basic cell biology pre-metamorphosis and post-metamorphosis. Therefore, we used single-cell RNA sequencing to obtain the cell states of larvae and juveniles of Heliocidaris crassispina.
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6. GSE310028 以单细胞分辨率解析肥胖和骨关节炎中跨组织的索玛鲁肽依赖性转录组反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、transcriptome
• 📝 描述：Contributors : Yuchen Tian ; Junjie GaoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe clinical observations of semaglutide cannot be fully explained by the classical GLP-1R signaling, indicating that semaglutide may exert its effects through a more complex multi tissue regulatory network. Based on this background, this study utilized high-fat diet (HFD) - induced obesity and surgery induced osteoarthritis (OA) models to simulate metabolic joint complications. By systematically collecting peripheral blood (PB), bone marrow (BM), liver, brain tissue, and skeletal muscle from C57BL/6 mice, and integrating 10x Genomics single-cell transcriptome sequencing, we constructed a cross tissue cell atlas that covers the immune, nervous, and motor metabolic systems under treatment with semaglutide. In addition, we compared the gene expression disturbances in mouse tissues after administration of semaglutide. Through these comparisons, we can more accurately decipher the specific effects of semaglutide treatment on gene expression in mice, thereby gaining a deeper understanding of their mechanisms of action in vivo.
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7. GSE277368 SIRT2 和 MOF 之间的功能拮抗作用调节细胞周期进程和基因组稳定性 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、genome
• 📝 描述：Contributors : Maria Espinosa-Alcantud ; Gerard Martinez-Cebrian ; Anna Marazuela-Duque ; Alejandro VaqueroSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusThe control of G2/M transition is a key event for genome stability as it ensures proper completion of DNA replication and repair before progressing into mitosis. One of the key regulators of G2/M checkpoint is the H4K16Ac-acetyltransferase MOF, which plays a major role in chromatin structure, gene expression, DNA damage and genome stability. Here we show that SIRT2, a member of the sirtuin family of NAD+-dependent deacetylases, antagonizes the role of MOF in G2/M. SIRT2 promotes specific inactivation of MOF through deacetylation and degradation of MOF, which results in re-expression of G2/M cell-cycle genes regulated by MOF. Underscoring the functional relevance of this antagonism, both factors play opposed roles in the deposition of the key mark H4K20me1 during G2/M. Consistently, loss of MOF in wt but not in SIRT2-/- cells induces a genome-wide deregulation of H4K20me1 distribution, which results in a premature loading of condensins. Our studies suggest that the G2/M checkpoint is shaped by the balance between both factors and involves condensing regulation and underscores the role of sirtuins in cell cycle control and genome stability under stress.
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8. GSE277367 SIRT2 和 MOF 之间的功能拮抗作用调节细胞周期进程和基因组稳定性 [ChIP-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：ChIP-seq、genome
• 📝 描述：Contributors : Maria Espinosa-Alcantud ; Gerard Martinez-Cebrian ; Anna Marazuela-Duque ; Alejandro VaqueroSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiens ; Mus musculusThe control of G2/M transition is a key event for genome stability as it ensures proper completion of DNA replication and repair before progressing into mitosis. One of the key regulators of G2/M checkpoint is the H4K16Ac-acetyltransferase MOF, which plays a major role in chromatin structure, gene expression, DNA damage and genome stability. Here we show that SIRT2, a member of the sirtuin family of NAD+-dependent deacetylases, antagonizes the role of MOF in G2/M. SIRT2 promotes specific inactivation of MOF through deacetylation and degradation of MOF, which results in re-expression of G2/M cell-cycle genes regulated by MOF. Underscoring the functional relevance of this antagonism, both factors play opposed roles in the deposition of the key mark H4K20me1 during G2/M. Consistently, loss of MOF in wt but not in SIRT2-/- cells induces a genome-wide deregulation of H4K20me1 distribution, which results in a premature loading of condensins. Our studies suggest that the G2/M checkpoint is shaped by the balance between both factors and involves condensing regulation and underscores the role of sirtuins in cell cycle control and genome stability under stress.
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9. GSE329809 体重减轻对腹部皮下脂肪组织代谢过程的影响：一项使用总RNA二代测序的探索性研究

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolic、sequencing
• 📝 描述：Contributor : Tom A HuyghebaertSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensObesity induces profound alterations in adipose tissue biology, yet the extent to which these transcriptional changes resolve after weight loss remains unclear. This exploratory study investigated molecular remodeling of abdominal subcutaneous adipose tissue (SAT) in formerly obese individuals.
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10. GSE292054 局部聚糖工程通过抗原交叉呈递诱导全身抗肿瘤免疫反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、antigen
• 📝 描述：Contributors : Natalia Mantuano ; Michael T Sandholzer ; Heinz LäubliSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusThe advent of immune checkpoint inhibition (ICI) has revolutionized cancer treatment, yet response rates remain modest, and mechanisms of resistance are poorly understood. Recent studies highlight the role of sialic acid-containing glycans and their interaction with sialic acid-binding immunoglobulin-like lectin (Siglec) receptors in the tumor microenvironment, offering a promising avenue for cancer immunotherapy. Myeloid cells, particularly tumor-associated macrophages (TAMs), mediate sialic acid-mediated immune suppression via Siglec receptors. Different approaches, including antibodies, sialidases, and glycomimetics, can block the sialic acid-Siglec pathway, with promising results in preclinical models and ongoing clinical trials. However, achieving sufficiently high intratumoral sialidase levels without systemic toxicity remains a challenge.
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1. 俄勒冈健康与科学大学科学家利用先进的测序技术开发口腔感染治疗方法

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：RNA sequencing with nanopore technology helps reveal how oral bacteria regulate genes, form biofilms, and may be targeted with more precise antimicrobial strategies…
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2. 纳米孔RNA测序在m6A检测中的基准测试

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：RNA sequencing with nanopore technology improves m6A modification mapping when paired with calibrated workflows, helping reveal cell-specific RNA regulation in fibroblasts and neurons…
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• GSE293172 先锋因子协调组织特异性粘连蛋白-NIPBL染色质进入和3D基因组组织[ChIP-seq]
• GSE293073 先锋因子协调组织特异性粘连蛋白-NIPBL染色质进入和3D基因组组织[Hi-C]
• GSE293072 先锋因子协调组织特异性粘连蛋白-NIPBL染色质进入和3D基因组组织[ATAC-seq]
• GSE292700 人类肺腺癌中 BRCA1 和 BRCA2 体细胞突变引起的肿瘤微环境的分子结构
• GSE328042 野生型或GPNMB敲除基因型的iPSC衍生小胶质细胞（iMicroglia）的RNA-seq分析
• GSE327216 循环 miR-22 预测 TACE 疗效并靶向肝细胞癌中的 WEE1
• GSE319320 ADAR1沉默对人单核细胞来源巨噬细胞的影响 II
• GSE318630 表达 GZMK 的组织驻留记忆 CD8+ T 细胞介导人类肠道急性移植物抗宿主病 [INT]
• GSE317159 RNA解旋酶Dhx29对生发中心反应和浆细胞分化的翻译控制[ATAC-seq]
• GSE290409 母体孕前体重过轻通过胎盘DNA甲基化改变诱发胎儿生长受限
• GSE284015 GZMK表达的组织驻留记忆CD8+ T细胞介导人类肠道急性移植物抗宿主病
• GSE307061 共生体靶向肽增强固氮作用而不发生终末分化 [植物 RNA-seq]
• GSE301558 脑 FGF2 和 NCAM1 促进年轻和老年宿主中 ER+ 乳腺癌脑转移的 FGFR1 依赖性进展 [NanoString_DSP]
• GSE301400 脑 FGF2 和 NCAM1 促进 FGFR1 依赖的 ER+ 乳腺癌脑转移在年轻和老年宿主中的进展
• GSE274884 CTCF 以环依赖性和非依赖性方式调节基因表达 [Hi-C]
• GSE274792 CTCF 以环依赖性和非依赖性方式调节基因表达 [ChIP-Seq]
• GSE274789 CTCF 以环依赖性和非依赖性方式调节基因表达 [ATAC-Seq]
• GSE274773 CTCF 以环依赖性和非依赖性方式调节基因表达 [RNA-seq]
• GSE329776 雌性大鼠背侧和腹侧海马体在不同年龄和胎次下的批量 RNA 测序
• GSE299400 HT-29细胞中CUL4A敲低后的转录组改变
• GSE296657 EONPs处理的BMDM细胞的RNA测序
• GSE328108 小鼠模型中自噬的可逆抑制揭示了神经元的恢复力
• GSE305103 RNA-seq 分析了经科里林处理和对照的 C57BL/6J 小鼠的肝脏、肾脏和股四头肌样本。
• GSE293071 先锋因子调控组织特异性黏连蛋白-NIPBL染色质进入和3D基因组组织
• GSE293070 先锋因子协调组织特异性粘连蛋白-NIPBL染色质进入和3D基因组组织[4C-Seq]