详细内容（全部5条）

1. ⭐ GSE250248 RNA-seq 分析从小鼠皮下 Lewis 肺癌 (LLC) 肿瘤中分离的肿瘤浸润效应 T 细胞

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、carcinoma、RNA-seq
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe goal of this study was to analyze differences in gene expression between tumor-infiltrating effector T cells with higher or lower uptake of the 2-deoxyglucose analog 2-NBDG in vivo. Mice bearing subcutaneous LLC tumors were injected intravenously with the 2-deoxyglucose analog 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195). 2-NBDG was used at 25 mg/kg of mouse body weight; 3.33 mg/ml in PBS. 2-NBDG was allowed to circulate for 15 min before euthanizing the mice and excising the tumors to isolate effector (CD44+, CD62L-negative T cells) TILs. The 15% of effector CD4 or CD8 TILs with the highest 2-NBDG staining were sorted as 2-NBDG Hi cells, and the lowest 15% were sorted as 2-NBDG Lo. 4 independent mice were used. For each population, a pool of 2000 cells (500 cells per mouse) was done. RNA of the respective pools was extracted with the RNeasy Microkit (Qiagen, Catalog: 74004), and cDNA libraries were prepared from poly A RNA (mRNA) using the Smart-Seq2 single-cell protocol (Picelli, et al., 2014, Nat. Protoc. 9, 171–181) with some modifications.
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2. GSE178815 NuRD 介导的 CTCF-TET 串扰协调表观基因组重编程和基因组结构

• ✍️ 作者：未知作者
• 🏷️ 关键词：genome、epigenome
• 📝 描述：Series Type : Methylation profiling by high throughput sequencing ; Other ; Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Escherichia coli ; Homo sapiens ; Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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3. GSE329398 CD109 在心脏发育过程中于内皮细胞和心内膜细胞亚群中动态表达，在体外调节内皮细胞行为，但并非心血管形态发生所必需。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cardiovascular
• 📝 描述：Contributors : Andrew B Harvey ; Jeremy L Barth ; Andy WesselsSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCD109 encodes a GPI-linked glycoprotein that acts as a signaling modulator in the TGF-β pathway. CD109 has emerged in several genome-wide association studies as linked to coronary artery disease, myocardial infarction, and angina pectoris. Heterozygous loss-of-function mutations in CD109 have also been reported in patients with congenital heart defects, suggesting potential developmental relevance, though CD109 has never been investigated in the context of cardiovascular development. We previously identified Cd109 upregulation in murine atrioventricular valves undergoing myxomatous degeneration following a reduction of epicardial-derived cells. Here, we characterize Cd109 expression in the murine cardiovascular system and assess its function during development using in vitro and in vivo approaches. We found that Cd109 is strongly expressed in the endothelium of the coronary vasculature and in an endocardial-derived subpopulation in the atrioventricular valves. This expression persists through key stages in cardiovascular development. Western blotting and immunostaining confirm endothelial expression in heart and lung tissues. siRNA knockdown of CD109 in primary human endothelial cells led to dysregulation of vascular development pathways and decreased tube formation capacity. We generated Tie2Cre;Cd109fl/fl mice, eliminating Cd109 expression from heart and lung tissues without overt morphological consequences. Together, these results establish Cd109 as a useful marker of coronary vasculature in the heart and indicate that endothelial lineage-specific deletion of Cd109 does not cause gross morphological defects during cardiac development. The transcriptional and functional changes observed in endothelial cells in vitro suggest that additional studies will be necessary to define contexts in which Cd109 influences vascular biology.
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4. GSE329021 野生型和twist突变体海葵（Nematostella vectensis）头部组织的批量RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：Contributors : Patricio Ferrer Muguia ; Ulrich TechnauSeries Type : Expression profiling by high throughput sequencingOrganism : Nematostella vectensisThe basic helix-loop-helix transcription factor Twist plays roles in mesodermal development and tissue morphogenesis. To assess transcriptional changes associated with loss of Twist function, we performed bulk RNA-seq on head tissue, including tentacles, from wild-type and twist mutant Nematostella vectensis animals exhibiting neoplasm-like development.
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5. GSE319204 克罗恩病患者炎症和非炎症结肠组织的单细胞RNA分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：scRNA
• 📝 描述：Contributor : Jie ZhaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSingle-cell RNA sequencing was performed on colonic tissues from Crohn’s disease patients, comparing inflammatory and non-inflammatory regions. The dataset characterizes cell-type composition and inflammation-associated transcriptional changes at single-cell resolution.
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详细内容（全部1条）

1. 增加一种蛋白质的摄入有助于大脑对抗阿尔茨海默病。

• ✍️ 作者：未知作者
• 🏷️ 关键词：Alzheimer
• 📝 描述：Scientists have discovered a way to help the brain clean itself of harmful Alzheimer’s plaques by activating its own support cells. By increasing a protein called Sox9, researchers were able to boost the activity of astrocytes, star shaped cells that help maintain brain health. In mice that already showed memory problems, this approach reduced plaque buildup and preserved cognitive function over time.
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