详细内容（前10条）

1. ⭐ GSE307142 肿瘤唾液酸化调控乳腺癌中有效的抗癌免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBreast cancer is the most prevalent cancer among women, yet immune checkpoint inhibitors remain largely ineffective in the majority of patients. To better understand the mechanisms underlying immune evasion in breast cancer, we analyzed two independent clinical cohorts and identified a negative association between tumor sialylation and T cell infiltration across molecular subtypes, most prominently in luminal B tumors. In preclinical models, both genetic and pharmacologic inhibition of tumor cell sialylation reshape the tumor microenvironment by reducing neutrophil and PMN-MDSC infiltration, while CD8⁺ effector and Tcf7⁺ memory T cells are increased within the mammary tumors. Mechanistically, sialylation enhances the serum half-life of granulocyte colony-stimulating factor (G-CSF), a key driver of neutrophilic immunosuppression, and dampens tumor cell immunogenicity by limiting MHC-I surface expression. Disruption of sialylation sensitizes resistant mammary tumors to CD8⁺ T cell-mediated killing and synergizes with anti-PD-1 therapy in multiple breast cancer models. Analysis of patient datasets further reveals a sialylation gene signature enriched in breast tumors, especially of the luminal B subtype, that correlates with increased neutrophils and poor CD8⁺ T cell infiltration. Our findings establish tumor cell sialylation as a central regulator of immune evasion in breast cancer, offering a therapeutic vulnerability to unlock anti-tumor immunity and improve responsiveness to immune checkpoint inhibitors.
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2. ⭐ GSE328617 早期营养失衡通过肠道菌群失调和代谢抑制损害结肠上皮再生

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolic、gut、regex:gut(-?microbiome)?
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusChildhood malnutrition represents a major global health burden that extends beyond caloric deficiency to include dietary imbalance. The early-life gut microbiome functions as a dynamic ecosystem whose structure and metabolic output are intimately linked to host development, yet how nutritional imbalance disrupts this ecosystem and its integration with the host remains poorly understood.
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3. GSE245231 TSA 处理的 A549 细胞的表观遗传谱分析 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：ATAC-seq、epigenetic
• 📝 描述：Contributors : Lifei Ma ; Huiyang Li ; Xiaoman Wang ; Hou-Zao ChenSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensCellular senescence (CS) refers to a stable arrest of the cell cycle characterized by an altered profiles of gene expression networks. However, a reliable strategy to quantify senescent levels is lacking. Here we developed a machine learning-based tool (Predictive Cellular Senescence Model, PreCSenM) that addresses this issue by defining senescent levels as CS score through incorporating senescent features from multiplatform data. While applying this model to predict clinical outcomes in patients with lung adenocarcinoma (LUAD), PreCSenMo identified that HDAC inhibitors (HDACi) can trigger LUAD cellular senescence. To reveal the epigenetic profiling dynamics of HDACi-induced senescence in lung cancer cells, we performed ATAC-seq of A549 treated with TSA.
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4. GSE327807 具有不同神经系统兴奋性的大鼠品系海马转录组特征

• ✍️ 作者：未知作者
• 🏷️ 关键词：nervous
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusBulk RNA sequencing of the hippocampus in male rats from two selectively bred strains differing in nervous system excitability: LT (low threshold, high-excitability) and HT (high threshold, low-excitability). The aim was to characterize baseline interstrain transcriptomic divergence in intact animals and identify major functional themes associated with inherited differences in excitability.
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5. GSE320438 一种新型的基于纳米抗体的TCR样CAR-T疗法靶向PRAME治疗急性髓系白血病

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia
• 📝 描述：Contributors : Xintong Liu ; Jianfeng Li ; Xue Li ; Xuejiao Yang ; Jianqing Mi ; Wenbo Wang ; Yueying WangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensChimeric antigen receptor (CAR) T-cell immunotherapy in acute myeloid leukemia (AML) remains challenging, primarily due to the lack of specific cell surface antigens that are highly expressed on leukemic blasts but largely absent on hematopoietic stem/progenitor cells and healthy tissues. TCR-like CAR-T therapy targeting intracellular antigens provides a novel strategy for the treatment of AML. In this study, we aimed to develop nanobodies targeting the intracellular antigen PRAME and to establish a novel nanobody-based TCR-like CAR-T cell therapy directed against the peptide-MHC complex PRAME425-433/HLA-A2.
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6. GSE263342 CNOT3 通过破坏 Tbx21 和 Rorc mRNA 的稳定性来维持 ILC2 的完整性 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：Contributor : Megumi TatematsuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusImmune responses are initiated by activation of innate lymphoid cells (ILCs), T-bet-dependent natural killer and ILC1 cells, GATA-3-dependent ILC2 cells, and RORγt-dependent ILC3 cells. Despite accumulated knowledge of translational regulation in ILCs, comprehensive impact of mRNA decay on ILCs has not been clarified yet. CNOT3 is a main subunit of the CCR4-NOT complex which mainly destabilizes mRNAs by poly (A) deadenylation in eukaryotes. Here, we showed that CNOT3 is required for cell survival of all ILCs and that the absence of CNOT3 promoted T-bet and RORγt expression due to increased stability of Tbx21 and Rorc mRNAs in ILC2 cells and enabled them to produce granzyme B and IL-22. Likewise, Th1 and Th17 skewing was induced in CD4 T cells lacking CNOT3. Furthermore, CNOT3 in each ILC subset contributes to their type-specific immune responses. Thus, the CCR4-NOT complex controls ILC differentiation by inhibiting the potential for type 1 and 3 immunity.
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7. GSE305655 RNA-seq 数据，包括用 HDAC8 抑制剂 22d 10 μM 处理和对照处理的 MV4-11、Kasumi-1 和 KG-1 细胞。

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：Contributors : Wancheng Guo ; Lianjun Zhang ; Yu-Hsuan Fu ; Ya-Huei KuoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe performed RNA sequencing on MV4-11, Kasumi-1, and KG-1 leukemia cell lines treated with HDAC8 inhibitor 22d at 10 μM and their respective control groups. The sequencing reads were processed to generate gene-level count matrices. This dataset provides processed count data for downstream gene expression analysis and comparison between treated and control conditions.
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8. GSE328779 兔非纤维化基质伤口再生单细胞RNA测序表征

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：Contributors : Borner Katherine ; Yu Ze ; Xing Chao ; Petroll W. MatthewSeries Type : Expression profiling by high throughput sequencingOrganism : Oryctolagus cuniculusFollowing injury or surgery, quiescent stromal keratocytes can transition into fibroblasts or myofibroblasts leading to either transient or protracted corneal haze. In this study, we investigate the transcriptional changes associated with non-fibrotic wound healing using a transcorneal freeze injury (FI) in the rabbit, which induces full-thickness stromal cell loss without inducing keratocyte-myofibroblast transformation. In control corneas, scRNA-seq revealed multiple clusters expressing markers associated with keratocyte identity (e.g. KERA, LUM, DCN, and ALDH1A1), suggesting heterogeneity in stromal keratocytes in the uninjured stroma. On day 7 after FI, in vivo imaging revealed elongated cells with increased backscatter, consistent with fibroblast migration into the wounded region. Using scRNA-seq, two additional clusters expressing fibroblast markers were also identified. These clusters retained many markers consistent with keratocyte identity, and trajectory analysis demonstrated a continuous progression from quiescent keratocytes to fibroblasts. Both fibroblast clusters had elevated expression genes encoding tenascin C (TNC), claudin 5 (CLDN5), developmental proteoglycans (e.g., BGN, ASPN, VCAN), and cytoskeletal genes (MYL9, MYH10, CDH11), but did not express markers of myofibroblast transformation. Together these genes suggest a mechanically active but non-fibrotic phenotype. One of the two fibroblast clusters also expressed genes related to cell proliferation. By day 28, fibroblastic gene expression was reduced, consistent with resolution of wound healing. These findings define the transcriptional dynamics of intrastromal cell migration following FI and reveal a transient fibroblastic state that supports wound repopulation without fibrosis. Understanding this non-fibrotic repair mechanism could inform strategies to prevent scarring following corneal surgery or injury.
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9. GSE328758 靶向细胞周期蛋白K-CDK12与ATR抑制剂协同作用，通过限制RPA染色质加载来抑制三阴性乳腺癌

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Contributors : Eun-Bee Choi ; Sophia Podeszwa ; Geoffrey I ShapiroSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCyclin K-CDK12 inhibition has emerged as a promising therapeutic strategy to induce homologous recombination deficiency (HRD). However, clinical and genomic studies have not clearly linked CDK12 inactivation to HRD. Here, we demonstrate that cyclin K-CDK12 depletion drives a replication stress (RS)-associated vulnerability through RPA regulation. A DNA damage response-focused CRISPR screen identified DNA replication factors and ATR activators as top sensitizers to cyclin K degradation. Consistently, cyclin K-CDK12 depletion synergized with ATR inhibition in triple-negative breast cancer (TNBC) models. Cyclin K degradation reduces RPA chromatin loading and impairs ATR activation, compromising cellular tolerance to RS and increasing susceptibility to ATR inhibition. We show that CDK12-mediated phosphorylation of CDC5L is required for RPA chromatin loading and mediates resistance to combined cyclin K and ATR depletion. Our findings identify cyclin K-CDK12 as a critical regulator of RPA dynamics and support its inhibition as rational therapy for RS-high tumors, including TNBC.
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10. GSE328649 谱系 2-北京结核分枝杆菌菌株在感染后早期抑制卡介苗训练的先天免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity
• 📝 描述：Contributors : Naomi Daniels ; Todia Setiabudiawan ; Taru Dutt ; Philip Hill ; Marcela Henao-Tamayo ; Reinout van Crevel ; Joanna KirmanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensGenetically distinct lineages of Mycobacterium tuberculosis differ in their virulence, transmissibility, and immune evasion capacity. The modern Lineage 2-Beijing (L2-B) clade of Mtb, which is highly prevalent in Asia and now globally distributed, is associated with resistance to the bacille Calmette Guerin (BCG) vaccine. Using a murine BCG vaccination model followed by aerosol challenge with geographically matched L2-B or Lineage 4 clinical isolates, we examined how L2-B circumvents vaccine-induced immunity. BCG-trained alveolar macrophages showed impaired control of L2-B growth in vitro. Spatial transcriptomic profiling of infected lungs revealed broad transcriptional reprogramming, including selective suppression of pathways essential for macrophage activation and trained innate immunity. Similar immune dysregulation was observed in peripheral blood from BCG-vaccinated household contacts of L2-B smear-positive TB patients. Together, these findings define mechanisms by which L2-B evades immune control, highlight key components of BCG-induced protection, and support re-evaluation of current TB vaccine strategies.
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1. ⭐ 哈佛大学科学家通过隐性炎症触发机制，将肠道细菌与抑郁症联系起来。

• ✍️ 作者：未知作者
• 🏷️ 关键词：bacteria、inflammation、regex:bacter(ia|ial|ium)、gut、regex:gut(-?microbiome)?
• 📝 描述：A gut bacterium may be quietly fueling depression through an unexpected chemical twist. Researchers found that when Morganella morganii interacts with a common pollutant, it produces a molecule that triggers inflammation—something strongly linked to depression. This finding helps explain how gut microbes can influence brain health at a molecular level. It also raises the possibility of new treatments that target the immune system rather than just the brain.
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2. 石墨烯能杀死有害细菌“超级细菌”，但不会伤害人体细胞。

• ✍️ 作者：未知作者
• 🏷️ 关键词：bacteria、regex:bacter(ia|ial|ium)
• 📝 描述：Scientists have uncovered how graphene oxide pulls off a remarkable trick: it hunts down and destroys harmful bacteria while leaving human cells completely unharmed. By targeting a molecule found only in bacterial membranes, this ultra-thin carbon-based material acts with laser-like precision—offering a powerful new alternative to traditional antibiotics. Even more exciting, it works against drug-resistant “superbugs,” promotes faster wound healing, and keeps its antibacterial strength even after repeated washing.
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• GSE328646 通过计算透明化技术对炎症性肠道类器官进行图像追踪
• GSE328626 棕榈酸处理的人类原代毛囊干细胞转录组分析
• GSE295700 FBXL6 通过 K63 连接的泛素化稳定 ENO1，从而促进膀胱癌进展