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1. GSE327564 番茄丁香假单胞菌DC3000双组分调控突变体cvsR在拟南芥早期感染和早期暴露于模式触发免疫过程中的植物体内转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity、transcriptome
• 📝 描述：Contributors : Hsiao-Chun Chen ; Brian Howard KvitkoSeries Type : Expression profiling by high throughput sequencingOrganism : Pseudomonas syringae pv. tomato str. DC3000We extracted P. syringae transcriptome from infected A. thaliana tissue under naive or PTI preactivated tissue by flg22 treatment, through physical separation by low speed centrifugation at 5 hours post inoculation. We compared the transcriptome of wildtype strain and mutant strain of cvsR, a previous identified two component regulator. This study aim to understand bacterial response to PTI and the role of cvsR regulon in plant.
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2. GSE327517 靶向 UBE2F 可诱导增强 CD8 T 细胞免疫的韧性程序

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity、T cell
• 📝 描述：Contributor : Xiaonan MaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCD8 memory T cells (TMEM) and exhausted T cells (TEX) are essential for host defense against infection and cancer, yet their therapeutic potential is often limited by insufficient persistence and sustained functional capacity. Strategies to enhance the longevity of both populations remain scarce. Here, we demonstrate that ablation of UBE2F, a neddylation E2 enzyme, induces a resilience program in CD8 T cells that operates across both TMEM and TEX compartments, resulting in improved viral and tumor control. This resilience state is characterized by enhanced self-renewal and survival without perturbing the conventional CD8 T cell differentiation trajectories. Mechanistically, UBE2F deficiency inhibited neddylation of CUL5, leading to accumulation of JUNB and upregulation of IL-2Rβ. The increased IL-2Rβ expression hypersensitizes CD8 T cells to physiological IL-15, thereby conferring the resilience features. Together, these findings identify the UBE2F-CUL5-JUNB-IL-2Rβ axis as a conserved post-translational mechanism regulating CD8 T cell longevity across memory and exhausted states, providing a novel strategy for enhancing antiviral and antitumor immunity.
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3. GSE327514 YY-15 处理的卵巢癌细胞的高通量测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、sequencing
• 📝 描述：Contributors : Yeying He ; Jiaying TONG ; Xiaohui ZhengSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAfter treating human ovarian cancer cells A2780 and ES2 with YY-15, high-throughput sequencing technology was employed. The transcriptome analysis revealed that YY-15 does not exert its anti-tumor effect solely through telomere dysfunction, but instead simultaneously regulates cell cycle, senescence, apoptosis, and metabolic-related signaling pathways, thereby synergistically inducing the death of ovarian cancer cells.
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4. GSE327495 靶向 STAT3 介导的脂质代谢重编程克服急性髓系白血病的化疗耐药性

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、metabolism
• 📝 描述：Contributors : Keren Peng ; Jianshan Mo ; Zhenjiao Yang ; Wen DingSeries Type : Expression profiling by high throughput sequencingOrganism : HomoChemotherapy resistance and intolerance pose significant challenges in the effective treatment of leukemia. Among the distinctive features associated with chemoresistance, metabolic reprogramming emerges as a key factor. In this study, we unveil a novel mechanism contributing to chemoresistance in leukemia, specifically highlighting the pivotal role of altered lipid homeostasis.We found that multiple lipid metabolism processes are aberrantly activated in Ara-C resistant AML cells, with upregulation of JAK-STAT3 signaling pathways and multiple enzymes in the process of fatty acid metabolism. A potent, and highly selective STAT3 inhibitor, W1307, was designed, and exhibited significant anti-tumor activities in vitro and in vivo. Genetic and pharmacological inhibition of STAT3 leads to inhibition of lipid synthesis and catabolism, resulting lipids metabolic disorder. Mechanistically, STAT3 binds to consensus DNA response elements in the promoters of the lipid metabolism associated genes (SREBP1, CPT2) and regulates their expression. Furthermore, inhibition of STAT3 enhances the anti-tumor effect of Ara-C and sensitizes resistant AML cell line to Ara-C through disrupting lipid homeostasis and triggering lipotoxicity. Collectively, our work illuminates the pivotal role of lipid metabolism adaptation driven by STAT3 in chemoresistance. The identification of W1307 as a promising candidate compound underscores its potential as a therapeutic intervention in leukemia treatment and overcoming chemoresistance.
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5. GSE327488 雪旺细胞与 CD4+T 细胞的相互作用促进结直肠癌神经周围侵袭的进展 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、RNA-seq
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBackground: Perineural invasion (PNI) is a common pathological feature associated with poor prognosis of colorectal cancer (CRC). However, the mechanisms underlying PNI formation and its relationship with CD4+T cells within the tumor immune microenvironment remain unclear. Aims: This study investigated the role of CD4+T cells in the development and progression of PNI in CRC by examining the interactions between Schwann cells (SCs), primary glial cells in peripheral nerves, and CD4+T cells. Methods: Single-cell RNA sequencing, spatial transcriptomics, and multiplex immunohistochemistry were used to profile the characteristics and spatial relationship between SCs and CD4+T cells in PNI CRC tissues. This study established an SCs-CD4⁺T-cells co-culture system and used flow cytometry, transcriptomic sequencing, proteomics, and ATAC-seq to investigate the mechanisms of their interaction. The key findings were validated in mouse models of sciatic nerve invasion and subcutaneous tumor formation. Finally, the origin of PNI was explored by co-culturing SCs with CRC cells. Results: The results revealed the presence of a distinct heat shock protein family A member 6 (HSPA6)+CD4+T cell subset—CD4+Tstr cells—in CRC PNI tissues. These cells are induced by SCs, further enhancing SCs’ migration and tumor-promoting capabilities while contributing to an immunosuppressive microenvironment. Moreover, SCs in contact with tumor cells possess the ability to recruit CD4+T cells, potentially representing a key mechanism underlying PNI formation. Conclusion: This study suggests that SCs near CRC tissues recruit CD4+T cells, promote their conversion to CD4+Tstr cells, establish an immunosuppressive microenvironment, and simultaneously enhance SCs’ migration and tumor-promoting capabilities, thereby accelerating PNI development.
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6. GSE276025 线粒体代谢调节树突状细胞的免疫原性反应[甲基化]

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、methylation
• 📝 描述：Contributors : Ignacio Heras-Murillo ; Stefanie K Wculek ; Josep Calafell-Segura ; Esteban Ballestar ; David SanchoSeries Type : Methylation profiling by arrayOrganism : Mus musculusActivation of dendritic cells (DCs) is linked to increased glycolysis. However, the role of the mitochondrial electron transport chain (ETC) in conventional DC subset function is poorly defined. Here, we revealed that immunogenic activation elevates late oxygen consumption in cDC1s, but not cDC2s. To investigate the relevance of the ETC in DC immunogenicity, we generated mice with impaired ETC complex III function in DCs. ETC complex III impairment attenuated poised or adjuvant-triggered cDC1 activation, migration and capability to prime T cells for anti-cancer immunity, while it had a mild effect on cDC2s. Mechanistically, loss of complex III in cDC1s led to a deregulated redox and metabolite balance causing DNA hypermethylation of PU.1-binding regions. This DNA hypermethylation diminished early stimulus-induced gene expression linked to immunogenic responsiveness of cDC1s, which was rescued via ectopic expression of alternative oxidase. Our findings show how the ETC differentially regulates the immunogenic function of cDC subsets.
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7. GSE276024 线粒体代谢调节树突状细胞的免疫原性反应 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、RNA-seq
• 📝 描述：Contributors : Ignacio Heras-Murillo ; Stefanie K Wculek ; David SanchoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusActivation of dendritic cells (DCs) is linked to increased glycolysis. However, the role of the mitochondrial electron transport chain (ETC) in conventional DC subset function is poorly defined. Here, we revealed that immunogenic activation elevates late oxygen consumption in cDC1s, but not cDC2s. To investigate the relevance of the ETC in DC immunogenicity, we generated mice with impaired ETC complex III function in DCs. ETC complex III impairment attenuated poised or adjuvant-triggered cDC1 activation, migration and capability to prime T cells for anti-cancer immunity, while it had a mild effect on cDC2s. Mechanistically, loss of complex III in cDC1s led to a deregulated redox and metabolite balance causing DNA hypermethylation of PU.1-binding regions. This DNA hypermethylation diminished early stimulus-induced gene expression linked to immunogenic responsiveness of cDC1s, which was rescued via ectopic expression of alternative oxidase. Our findings show how the ETC differentially regulates the immunogenic function of cDC subsets.
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8. GSE276020 线粒体代谢调节树突状细胞的免疫原性反应 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、ATAC-seq
• 📝 描述：Contributors : Ignacio Heras-Murillo ; Diego Mañanes ; Stefanie K Wculek ; David SanchoSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusActivation of dendritic cells (DCs) is linked to increased glycolysis. However, the role of the mitochondrial electron transport chain (ETC) in conventional DC subset function is poorly defined. Here, we revealed that immunogenic activation elevates late oxygen consumption in cDC1s, but not cDC2s. To investigate the relevance of the ETC in DC immunogenicity, we generated mice with impaired ETC complex III function in DCs. ETC complex III impairment attenuated poised or adjuvant-triggered cDC1 activation, migration and capability to prime T cells for anti-cancer immunity, while it had a mild effect on cDC2s. Mechanistically, loss of complex III in cDC1s led to a deregulated redox and metabolite balance causing DNA hypermethylation of PU.1-binding regions. This DNA hypermethylation diminished early stimulus-induced gene expression linked to immunogenic responsiveness of cDC1s, which was rescued via ectopic expression of alternative oxidase. Our findings show how the ETC differentially regulates the immunogenic function of cDC subsets.
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9. GSE327576 杏发酵过程中挥发性化合物动态变化的转录组学和代谢基础

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolic
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Wickerhamomyces anomalusAroma is one of the most important qualities that defines the appeal and value of the fermented fruit products. This study provides the first integrative analysis of volatile compound dynamics and transcriptomic regulations during apricot fermentation by Wickerhamomyces anomalus Using GC-MS, we tracked the temporal evolution of key aroma-active metabolites including s 3-hydroxy-2-butanone, 2,3-butanediol, benzaldehyde, isoamyl alcohol, and phenethyl alcohol across 0, 16, 32 ,48, and 64 h. these aromatic compounds exhibited a distinct accumulation pattern, peaking at 48h with concentrations of 11.14 μg/g, 1.28 μg/g, 6.13 μg/g, 29.12 μg/g, and 13.59 μg/g respectively. Metatranscriptomicprofiling revealed extensive differential gene expression at different time points of fermentation. KEGG pathway analysis revealed the mechanisms to produce 3-hydroxy-2-butanone and 2,3-butanediol, as well as the phenylalanine-dervided aromatic compounds. Gene annotation establish direct link between transcriptional regulation and volatile production, uncovering mechanistic connections between yeast metabolism and aroma development. The findings highlight W. anomalus as a promising non-conventional yeast for natural aroma enhancement, offering new biotechnological strategies to optimize flavor quality in fruit-based fermentations and functional foods.
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10. GSE327567 热休克因子 5 在小鼠精子发生过程中对粗线期进展和 LINE1 沉默至关重要 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：Contributors : Qi Sun ; Yuming Cao ; Zhiyi Chen ; Jin Shen ; Zejia Li ; Binyi Wang ; Muyang Cheng ; Jiemin Chen ; Weihao Gong ; Mengcheng Luo ; Yuanzhen Zhang ; Rong LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPachytene spermatocytes were isolated from adult wild-type and Hsf5 knockout mouse testes using STA-PUT velocity sedimentation. Single-cell suspensions were prepared by enzymatic digestion of seminiferous tubules with collagenase and trypsin. Cells were loaded onto a 4%–2%–0.5% BSA gradient and allowed to sediment for 1 hour and 45 minutes at 4°C. Fractions were collected, and pachytene spermatocytes were identified by SYCP3, γH2AX, and DAPI staining under microscopy. Enriched pachytene cells were pooled and processed for RNA-Seq.
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1. 新型牙膏可在预防牙龈疾病的同时，不杀死有益菌。

• ✍️ 作者：未知作者
• 🏷️ 关键词：bacteria、regex:bacter(ia|ial|ium)
• 📝 描述：Scientists have developed a new way to fight gum disease without wiping out the mouth’s helpful bacteria—a major shift from traditional treatments. Instead of killing everything, this targeted approach blocks only the harmful microbes that drive periodontitis, allowing beneficial bacteria to thrive and restore balance naturally.
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• GSE327496 Single-cell transcriptomic analysis of synovial tissues from aged mice
• GSE327142 Canavanine-based assay for gross chromosomal rearrangements reveals hotspots and genome instability modulating genes in fission yeast
• GSE308685 线粒体代谢调节树突状细胞的免疫原性反应
• GSE294066 DLX6 KD A549 细胞与对照 A549 细胞的 RNA-seq。
• GSE310252 基于转录组的胰腺导管腺癌切除标本分类提高了辅助治疗后总生存期的预后模型准确性
• GSE269572 利用人类多能干细胞生成稳定的心腔类心肌细胞 [scRNA-seq]
• GSE269571 利用人类多能干细胞生成稳定的心腔类心肌细胞 [bulk RNA-seq]