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1. ⭐ GSE287799 多胺生物合成途径的表观遗传调控可纠正 T 细胞功能障碍，从而增强肺癌的抗肿瘤免疫力 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、T cell、RNA-seq、epigenetic
• 📝 描述：Contributor : Hsing-Chen TsaiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensT cell exhaustion (TEX) represents a critical target for immunotherapy in cancer. Nevertheless, T cells exhibit diminished responsiveness to immune checkpoint inhibitors once they transition to a terminally-exhausted state. Here, we employed an epigenetic drug screen and identified BET inhibitors (BETis) as enhancers of effector functions in primary exhausted T cells from malignant pleural effusions in lung cancer patients. Transcriptomics, metabolomics, and ATAC-seq analyses revealed that BETis reinvigorate TEX by activating the polyamine biosynthesis pathway, expanding intracellular polyamine pools, and altering chromatin accessibility. Genetic and pharmacological inhibition of ornithine decarboxylase (ODC), a key enzyme in this pathway, abolished BETi-mediated immunopotentiation. Single-cell RNA-seq demonstrated BETis reduced terminal TEX while promoting progenitor TEX through activation of the MYC-ODC axis. BETi treatment or adoptive transfer of BETi-treated T cells suppressed malignant pleural effusion formation in a syngeneic lung cancer model. These findings highlight an epigenetic-metabolic approach to enhance TEX plasticity and offers insights for novel cancer immunotherapies.
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2. ⭐ GSE287798 多胺生物合成途径的表观遗传调控可纠正 T 细胞功能障碍，从而增强肺癌的抗肿瘤免疫力 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、T cell、ATAC-seq、epigenetic
• 📝 描述：Contributor : Hsing-Chen TsaiSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensT cell exhaustion (TEX) represents a critical target for immunotherapy in cancer. Nevertheless, T cells exhibit diminished responsiveness to immune checkpoint inhibitors once they transition to a terminally-exhausted state. Here, we employed an epigenetic drug screen and identified BET inhibitors (BETis) as enhancers of effector functions in primary exhausted T cells from malignant pleural effusions in lung cancer patients. Transcriptomics, metabolomics, and ATAC-seq analyses revealed that BETis reinvigorate TEX by activating the polyamine biosynthesis pathway, expanding intracellular polyamine pools, and altering chromatin accessibility. Genetic and pharmacological inhibition of ornithine decarboxylase (ODC), a key enzyme in this pathway, abolished BETi-mediated immunopotentiation. Single-cell RNA-seq demonstrated BETis reduced terminal TEX while promoting progenitor TEX through activation of the MYC-ODC axis. BETi treatment or adoptive transfer of BETi-treated T cells suppressed malignant pleural effusion formation in a syngeneic lung cancer model. These findings highlight an epigenetic-metabolic approach to enhance TEX plasticity and offers insights for novel cancer immunotherapies.
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3. GSE301970 Kras突变肠上皮细胞重组后10天的多组学分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：KRAS、regex:intestin(e|al)
• 📝 描述：Contributors : Amanda R Moore ; Sandra P Melo ; Frederic J de SauvageSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Mus musculusHere, we used multiome on flash frozen ileal tissue isolated from VilCre; Kras-mutant animals 10 day post-tamoxifen induction.
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4. GSE326693 沙门氏菌 SopB 抑制转录后调控的细胞因子释放，从而减少早期组织炎症并延缓疾病进展

• ✍️ 作者：未知作者
• 🏷️ 关键词：cytokine、inflammation
• 📝 描述：Contributors : Nour Diab ; Matthias Schmitz ; Kaiyi Zhang ; Mathias W. HornefSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSalmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) manipulates cellular processes through the translocation of effector molecules into the host cell cytosol. Using a recently established neonatal S. Typhimurium infection model, we provide functional insights into how Salmonella outer protein B (SopB) suppresses early mucosal tissue inflammation and prolongs host survival. Mechanistically, SopB prevents a disintegrin and metalloprotease 17 (ADAM17) activation, plasma membrane translocation and the release of membrane-bound TNFa from enterocytes and reduces epithelial secretion of IL-18 via mTOR-controlled secretory autophagy. This abolishes the early epithelial transcriptional response and reduces immune cell recruitment and programmed cell death-mediated mucosal barrier disruption delaying disease progression. The immunosuppressive effect of SopB is independent of the C-terminally encoded phosphatidylinositol phosphatase and phosphotransferase activity but requires an intact N-terminal domain. Thus, SopB suppresses the early, post-transcriptional regulation of epithelial cytokine release in an inositol phosphatase-independent manner likely promoting pathogen transmission.
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5. GSE326246 柠檬酸可维持T细胞干性和抗肿瘤免疫力

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity、T cell
• 📝 描述：Contributors : Wenhui Li ; Minmin Ge ; Ziyi Luo ; Minju Ni ; Lianjun ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMetabolic perturbations within the tumor microenvironment profoundly compromise the stem-like properties and functionality of CD8+ T cells. Elucidating the metabolic circuitry that maintains T cell stemness is therefore essential for rejuvenating tumor-infiltrating lymphocytes and enhancing immunotherapeutic efficacy. Here, we identify citraconate, an itaconate isomer, as a critical metabolite markedly depleted in CD8+ T cells under chronic antigen stimulation or hypoxia. Citraconate supplementation preserves stem-like properties, mitigates ferroptosis, and potentiates T cell-mediated antitumor efficacy. Mechanistically, citraconate sustains intracellular cAMP pool by suppressing phosphodiesterase (PDE1A/C) expression and maintaining mitochondrial integrity, thereby activating protein kinase A (PKA) signaling. This activation represses arachidonate-5-lipoxygenase (ALOX5) transcription, reducing arachidonic acid peroxidation. Clinically, reduced ALOX5 or PDE1A expression correlates with decreased T cell exhaustion and enhanced responses to immune checkpoint blockade (ICB) therapy. Our findings reveal the citraconate-mediated PDE1-cAMP-ALOX5 axis as a promising therapeutic target for potentiating cancer immunotherapy.
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6. GSE324222 高精子活力鹅的内分泌代谢支持和轴丝组装增强：来自单细胞RNA测序睾丸细胞异质性的启示

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolic、scRNA
• 📝 描述：Contributors : Hechuan Wang ; Kexin Cong ; Jiaxin Yin ; Ke Jiang ; Yunuo Liu ; Xiaofang Ren ; Ying Zhang ; Shengjun LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Anser cygnoidesWe used 10× Genomics single-cell RNA sequencing to investigate the cellular and transcriptional mechanisms underlying sperm motility differences in goose testes. Spermatogenesis depends on the coordinated interaction between germ cells and somatic cells in the testicular microenvironment, but the cellular basis of sperm motility variation remains unclear in geese. Here, we generated a single-cell transcriptomic atlas of testes from high- and low-sperm-motility groups and identified the major germ-cell and somatic-cell populations involved in spermatogenesis. These findings provide new insights into the molecular regulation of avian spermatogenesis and establish a valuable resource for understanding the mechanisms underlying male fertility in geese.
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7. GSE303719：从 Apc cKO 和 Kras 突变动物中分离的肿瘤的 snRNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：KRAS
• 📝 描述：Contributors : Amanda R Moore ; Sandra P Melo ; Frederic J de SauvageSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHere, we performed snRNAseq on flash frozen tumors isolated from VilCre; Apc fl/fl; Kras-mutant animals.
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8. GSE301967 对从 Apc cKO；Kras 突变动物中分离的肿瘤进行多组学分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：KRAS
• 📝 描述：Contributors : Amanda R Moore ; Sandra P Melo ; Frederic J de SauvageSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Mus musculusHere, we performed multiome on flash frozen tumors isolated from VilCre; Apc fl/fl; Kras-mutant animals.
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9. GSE301568 用单一药物 KRAS G12C 抑制、TEAD 抑制或 APC 恢复，或双重和三重组合治疗 AKPS 类器官对基因表达的影响。

• ✍️ 作者：未知作者
• 🏷️ 关键词：KRAS
• 📝 描述：Contributors : Amanda R Moore ; Thi Thu Thao Nguyen ; Sandra P Melo ; Frederic J de SauvageSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe have previously shown that single agent treatment with either divarasib (KRAS G12Ci) or APC restoration, results in cell plasticity. Here, we aim to evaluate the effects on combination treatment and if gene expression changes can be enhanced with TEAD inhbiiton.
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10. GSE301567 MEK抑制对KrasQ61R突变型Lgr5阳性肠道干细胞的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:intestin(e|al)
• 📝 描述：Contributors : Amanda R Moore ; Sandra P Melo ; Frederic J de SauvageSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe have previously shown that KRAS hyperactivation depletes the Lgr5-positive intestinal stem cells. Here, we aim to address whether reducing the MAPK pathway output, by using a MEK inhibitor (Cobimetinib) is sufficient to restore expression of Lgr5-positive intestinal stem cell markers.
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1. 科学家发现了一种加速大脑衰老的蛋白质——以及如何阻止它衰老。

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Scientists have uncovered a powerful new clue in the mystery of brain aging: a single protein called FTL1. In aging mice, higher levels of this protein weakened connections between brain cells and led to memory decline. But when researchers reduced FTL1, something remarkable happened — the brain began to recover, rebuilding lost connections and restoring memory performance.
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2. 科学家发现隐藏的脑细胞会促进致命癌症的生长

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists in Canada have uncovered a surprising weakness in glioblastoma, one of the deadliest brain cancers. They found that certain brain cells—once believed to only support healthy nerves—can actually help tumors grow by sending signals that strengthen cancer cells. When researchers blocked this communication, tumor growth slowed dramatically in lab models.
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• GSE301565 Kras 突变条件敲入等位基因中 Kras 同工型的表达。
• GSE301559 用单一药物 KRAS G12C 抑制或 APC 恢复或两者结合治疗 AKPS 类器官对染色质可及性的影响。
• GSE300617 模拟微重力模型通过 CD36 调控的机械代谢回路揭示膝骨关节炎的性别差异
• GSE326762 命运图谱揭示了具有 Cd3g 表达历史的 ILC2 细胞在产前至新生儿期的增殖浪潮 - RNA-Seq
• GSE326751 RNA-seq 分析了 VAMP8 基因敲除小鼠和野生型小鼠经 AngII 或生理盐水输注后的肾上腺主动脉 RNA 数据。
• GSE326704 ROS感应转录因子YchJ调控RssB-RpoS通路以保护沙门氏菌免受巨噬细胞的氧化攻击
• GSE326579 IL-17 信号通路除 TNFα 外，还介导炎症诱导的毛细血管渗漏
• GSE307183 FTO抑制剂会耗竭半胱氨酸代谢并增强非小细胞肺癌对放射治疗的敏感性
• GSE292341 果糖诱导代谢综合征及DHA和NR补充治疗的AD小鼠海马和下丘脑单细胞RNA测序
• GSE309188 苯丙氨酰-tRNA合成酶通过下调内源性线粒体双链RNA来平衡寿命和免疫力
• GSE298228 PLAGL1-KLF4-IRX5 轴促进下颌骨再生过程中骨膜祖细胞的成骨作用 [ATAC-seq]
• GSE279368 下颌骨再生过程中的细胞簇 [scRNA-seq]
• GSE279367 PLAGL1 在下颌骨成骨过程中的功能 [RNA-seq]