详细内容（前10条）

1. ⭐ GSE326837 空间转录组学揭示幼年特发性关节炎患者滑膜内的免疫-基质相互作用

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributor : Yomogida KentaroSeries Type : OtherOrganism : Homo sapiensJuvenile idiopathic arthritis (JIA) is the most prevalent chronic inflammatory arthritis of childhood, yet the spatial organization in the synovium remains poorly understood. Here, we perform subcellular-resolution spatial transcriptomic profiling of synovial tissue from patients with active JIA. We identify diverse immune and stromal cell populations and reconstruct spatially defined cellular niches. Applying a newly developed spatial colocalization analysis pipeline, we uncover microanatomical structures, including endothelial-fibroblast interactions mediated by NOTCH signaling, and a CXCL9/CXCR3 signaling axis between inflammatory macrophages and CD8+ T cells, alongside the characterization of other resident macrophage subsets. We also detect and characterize tertiary lymphoid structures marked by CXCL13/CXCR5 and CCL19-mediated signaling from Tph cells and immunoregulatory DCs, analogous to those observed in other autoimmune diseases. Finally, comparative analysis with rheumatoid arthritis reveals JIA-enriched cell states, including NOTCH3+ and CXCL12+ sublining fibroblasts, suggesting potentially differential inflammatory programs in pediatric versus adult arthritis. These findings provide a spatially resolved molecular framework of JIA synovitis and introduce a generalizable computational pipeline for spatial colocalization analysis in tissue inflammation.
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2. ⭐ GSE309213 HDAC 抑制可解锁肿瘤可塑性并增强 Myc 驱动的小细胞肺癌的免疫治疗反应 [ATAC-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、regex:immuno(logy|therapy|suppression)、ATAC-seq
• 📝 描述：Contributors : Azam Ghafoor ; Lorinc Prongor ; Linying Zhu ; Haoxuan YingSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusSmall Cell Lung Cancer (SCLC) is a highly aggressive malignancy, accounting for approximately 15% of all lung cancer cases. Characterized by low immunogenicity, SCLC may utilize epigenetic mechanisms to evade immune detection. Here, we demonstrate that entinostat, a class I histone deacetylase inhibitor (HDACi) upregulates immune-related genes in human SCLC cells. In vivo, we confirmed entinostat treatment increased expression of immunecheckpoint ligands and antigen presentation machinery in Myc-driven tumors in a Rb1/Trp53/MycT58A (RPM) SCLC mouse model, while shifting tumors from a neuroendocrine(NE)-high to a NE-low phenotype. Notably, combining entinostat with anti-PD-1 immunotherapy significantly enhances T-cell infiltration, suppresses tumor growth, and prolongs survival in RPM allograft models. These findings underscore the potential of entinostat to reprogram the immunological landscape and NE status of SCLC, enhance immune checkpoint blockade efficacy, and improve therapeutic outcomes.
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3. ⭐ GSE315534 原发性结直肠癌、匹配的邻近正常组织和配对的肝转移灶的单细胞RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、sequencing、single-cell
• 📝 描述：Contributors : Yueming Sun ; Junwei Tang ; Yifei Feng ; Xiaowei Wang ; Sheng YangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis study will perform single-cell RNA sequencing on primary tumor tissues (T) and matched adjacent non-tumor tissues (N) from six colorectal cancer patients, along with paired liver metastatic lesions (M) from three of these patients, to construct an integrated single-cell atlas spanning primary tumors, adjacent normal tissues, and distant metastases. This multi-region, patient-matched design enables systematic dissection of cellular heterogeneity, dynamic immune responses, and key molecular mechanisms driving metastasis in colorectal cancer.
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4. ⭐ GSE326210 一种用于并行T细胞受体克隆和检测的新型平台可实现抗新抗原肿瘤免疫疗法

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、T cell、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Alexander M Rowe ; Smriti Chaurasia ; Wenzhong Wei ; Lau Garcia-Dieguez ; Katherine Querry ; Jonathon Schiebel ; Christy Smolak ; Alexander Muralles ; Daniel Wikenheiser ; Kevin Quann ; Collin Pirner ; Kentin Codispot ; Mark J Shlomchik ; Warren D ShlomchikSeries Type : OtherOrganism : Mus musculusTumor-infiltrating CD8 cells recognize neoantigens created by tumor-specific mutations. Nonetheless, even after checkpoint inhibitor therapy, most patients progress. A deeper understanding of anti-tumor responses could facilitate development of better therapies. To enable such studies, we applied TCXpress, a high throughput platform that clones fully expressible TCRs from single cells into retro- or lenti- viral vectors without sequencing or gene synthesis, to study TCRs from CD8 cells infiltrating mouse MC38 tumors. We expressed cloned TCRs in reporter cells and interrogated TCR specificity by coculturing them with B6WT3 cells transduced with tandem minigenes encoding predicted neoantigens. We isolated TCRs reactive against epitopes from mutant Rpl18, ADPGK, PSMD2 and Zc3h7b along with self-reactive TCRs that recognize normal B6 and MC38 cells. Importantly, we successfully treated MC38-bearing mice with T cells transduced with anti-Rpl18 TCRs. These results establish a system that could be used to study many types of T cell responses and validates a therapeutic approach that could be tested in the clinic.
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5. ⭐ GSE309199 HDAC 抑制可解锁肿瘤可塑性并增强 Myc 驱动的小细胞肺癌的免疫治疗反应。

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Azam Ghafoor ; Lorinc Prongor ; Linying Zhu ; Haoxuan YingSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSmall Cell Lung Cancer (SCLC) is a highly aggressive malignancy, accounting for approximately 15% of all lung cancer cases. Characterized by low immunogenicity, SCLC may utilize epigenetic mechanisms to evade immune detection. Here, we demonstrate that entinostat, a class I histone deacetylase inhibitor (HDACi) upregulates immune-related genes in human SCLC cells. In vivo, we confirmed entinostat treatment increased expression of immunecheckpoint ligands and antigen presentation machinery in Myc-driven tumors in a Rb1/Trp53/MycT58A (RPM) SCLC mouse model, while shifting tumors from a neuroendocrine(NE)-high to a NE-low phenotype. Notably, combining entinostat with anti-PD-1 immunotherapy significantly enhances T-cell infiltration, suppresses tumor growth, and prolongs survival in RPM allograft models. These findings underscore the potential of entinostat to reprogram the immunological landscape and NE status of SCLC, enhance immune checkpoint blockade efficacy, and improve therapeutic outcomes.
• 🔗 查看原文

6. ⭐ GSE309192 HDAC抑制剂可激活肿瘤可塑性并增强Myc驱动的小细胞肺癌的免疫治疗反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Azam Ghafoor ; Lorinc Prongor ; Linying Zhu ; Haoxuan YingSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSmall Cell Lung Cancer (SCLC) is a highly aggressive malignancy, accounting for approximately 15% of all lung cancer cases. Characterized by low immunogenicity, SCLC may utilize epigenetic mechanisms to evade immune detection. Here, we demonstrate that entinostat, a class I histone deacetylase inhibitor (HDACi) upregulates immune-related genes in human SCLC cells. In vivo, we confirmed entinostat treatment increased expression of immunecheckpoint ligands and antigen presentation machinery in Myc-driven tumors in a Rb1/Trp53/MycT58A (RPM) SCLC mouse model, while shifting tumors from a neuroendocrine(NE)-high to a NE-low phenotype. Notably, combining entinostat with anti-PD-1 immunotherapy significantly enhances T-cell infiltration, suppresses tumor growth, and prolongs survival in RPM allograft models. These findings underscore the potential of entinostat to reprogram the immunological landscape and NE status of SCLC, enhance immune checkpoint blockade efficacy, and improve therapeutic outcomes.
• 🔗 查看原文

7. GSE326797 抗体介导的初级和次级体液免疫反应多样化

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、antibody
• 📝 描述：Contributors : Dennis Schaefer-Babajew ; Laurine Binet ; Gabriela S Silva Santos ; Chiara Ruprecht ; Lachlan P Deimel ; Mohamed A ElTanbouly ; Dounia Gharrassi ; Gabriella Lima dos Reis ; Clara Uhe ; Kai-Hui Yao ; Brianna Hernandez ; Parul Agrawal ; Anna Gazumyan ; Leonidas Stamatatos ; Harald Hartweger ; Michel C NussenzweigSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusHumoral immune responses diversify over time but whether secreted antibodies influence this process is unknown. Using antibody secretion-deficient mice this study shows a profound impact of secreted antibodies on the evolution of B cell diversity after vaccination.
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8. GSE325621 比较单细胞多组学分析揭示了介导自然视网膜衰老的进化保守和物种特异性细胞机制 [斑马鱼多组学_ATAC]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、single-cell
• 📝 描述：Contributors : Pin Lyu ; Isabella Palazzo ; Yang Jin ; Leah J. Campbell ; Clayton P. Santiago ; Rogger P. Carmen-Orozco ; Thanh Hoang ; Jared A. Tangeman ; Alexander D. Park ; Shawn H. Yang ; Jianming Shao ; Rui Chen ; David Hyde ; Jiang Qian ; Seth BlackshawSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Danio rerioBiological age is a major risk factor in the development of common degenerative retinal diseases such as age-related macular degeneration and glaucoma. To systematically characterize molecular mechanisms underlying retinal aging, we performed integrated single- cell RNA- and ATAC-Seq analyses of the retina and retinal pigment epithelium (RPE) across the natural lifespan in zebrafish, mice, and humans. By profiling gene expression and chromatin accessibility, we identified extensive cell type- and species-specific aging-dependent changes, with a much smaller number of broadly expressed and conserved genes that include regulators of inflammation and autophagy. We constructed predictive aging clocks for retinal cell types and observed dynamic, reversible shifts in cellular age following acute injury. Spatial transcriptomic analysis revealed region-specific aging signatures and proximity effects, with Müller glia exhibiting pro-rejuvenating influences on neighboring neurons. Targeted Müller glia-specific induction of Yamanaka factors reduced molecular age in rod photoreceptors and bipolar cells without altering glial age. Our findings define conserved and divergent regulatory and signaling pathways mediating retinal aging, highlighting Müller glia as potential therapeutic targets for combating age-associated retinal dystrophies.
• 🔗 查看原文

9. GSE325620 比较单细胞多组学分析揭示了介导自然视网膜衰老的进化保守和物种特异性细胞机制 [斑马鱼_多组 RNA]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、single-cell
• 📝 描述：Contributors : Pin Lyu ; Isabella Palazzo ; Yang Jin ; Leah J. Campbell ; Clayton P. Santiago ; Rogger P. Carmen-Orozco ; Thanh Hoang ; Jared A. Tangeman ; Alexander D. Park ; Shawn H. Yang ; Jianming Shao ; Rui Chen ; David Hyde ; Jiang Qian ; Seth BlackshawSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioBiological age is a major risk factor in the development of common degenerative retinal diseases such as age-related macular degeneration and glaucoma. To systematically characterize molecular mechanisms underlying retinal aging, we performed integrated single- cell RNA- and ATAC-Seq analyses of the retina and retinal pigment epithelium (RPE) across the natural lifespan in zebrafish, mice, and humans. By profiling gene expression and chromatin accessibility, we identified extensive cell type- and species-specific aging-dependent changes, with a much smaller number of broadly expressed and conserved genes that include regulators of inflammation and autophagy. We constructed predictive aging clocks for retinal cell types and observed dynamic, reversible shifts in cellular age following acute injury. Spatial transcriptomic analysis revealed region-specific aging signatures and proximity effects, with Müller glia exhibiting pro-rejuvenating influences on neighboring neurons. Targeted Müller glia-specific induction of Yamanaka factors reduced molecular age in rod photoreceptors and bipolar cells without altering glial age. Our findings define conserved and divergent regulatory and signaling pathways mediating retinal aging, highlighting Müller glia as potential therapeutic targets for combating age-associated retinal dystrophies.
• 🔗 查看原文

10. GSE325478 比较单细胞多组学分析揭示了介导自然视网膜衰老的进化保守和物种特异性细胞机制 [小鼠多组学_scRNA]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、single-cell
• 📝 描述：Contributors : Pin Lyu ; Isabella Palazzo ; Yang Jin ; Leah J. Campbell ; Clayton P. Santiago ; Rogger P. Carmen-Orozco ; Thanh Hoang ; Jared A. Tangeman ; Alexander D. Park ; Shawn H. Yang ; Jianming Shao ; Rui Chen ; David Hyde ; Jiang Qian ; Seth BlackshawSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBiological age is a major risk factor in the development of common degenerative retinal diseases such as age-related macular degeneration and glaucoma. To systematically characterize molecular mechanisms underlying retinal aging, we performed integrated single- cell RNA- and ATAC-Seq analyses of the retina and retinal pigment epithelium (RPE) across the natural lifespan in zebrafish, mice, and humans. By profiling gene expression and chromatin accessibility, we identified extensive cell type- and species-specific aging-dependent changes, with a much smaller number of broadly expressed and conserved genes that include regulators of inflammation and autophagy. We constructed predictive aging clocks for retinal cell types and observed dynamic, reversible shifts in cellular age following acute injury. Spatial transcriptomic analysis revealed region-specific aging signatures and proximity effects, with Müller glia exhibiting pro-rejuvenating influences on neighboring neurons. Targeted Müller glia-specific induction of Yamanaka factors reduced molecular age in rod photoreceptors and bipolar cells without altering glial age. Our findings define conserved and divergent regulatory and signaling pathways mediating retinal aging, highlighting Müller glia as potential therapeutic targets for combating age-associated retinal dystrophies.
• 🔗 查看原文

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1. Bostongene RNA转录组分析揭示晚期实体瘤中新的ADC靶点

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、transcriptome
• 📝 描述：RNA sequencing combined with multiomic analysis uncovers new therapeutic targets in advanced cancers, enabling identification of antibody drug conjugate options when DNA testing alone shows no actionable findings…
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2. MitoPerturb-Seq 可识别基因特异性的单细胞对线粒体 DNA 耗竭和异质性的反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell
• 📝 描述：RNA sequencing reveals how nuclear genes regulate mitochondrial DNA copy number and heteroplasmy, uncovering stress responses and potential therapeutic targets for mitochondrial…
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3. 科学家发现一种“分子粉碎机”，可帮助致命寄生虫逃避免疫系统的攻击。

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune
• 📝 描述：A deadly parasite responsible for sleeping sickness has been found using a surprisingly precise trick to stay hidden in the human bloodstream. Scientists discovered a protein called ESB2 that acts like a “molecular shredder,” cutting up specific genetic instructions as they are produced. This allows the parasite to flood its surface with protective proteins while suppressing other signals that might give it away.
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4. 多吃肉可能降低某些人患阿尔茨海默病的风险

• ✍️ 作者：未知作者
• 🏷️ 关键词：Alzheimer
• 📝 描述：A surprising new study suggests that genetics may change how diet affects brain health—especially when it comes to Alzheimer’s risk. Researchers found that older adults carrying high-risk APOE gene variants didn’t show the expected cognitive decline if they ate relatively high amounts of meat. In fact, those with these genes who consumed the most meat had slower cognitive decline and lower dementia risk, challenging conventional dietary advice.
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• GSE325477 单细胞多组学比较分析揭示了介导自然视网膜衰老的进化保守和物种特异性细胞机制 [小鼠多组学_scATAC]
• GSE313488 CTD 的“拉链”语法控制 RNA 聚合酶 II 转录周期的空间编程 [ChIP-Seq]
• GSE294295 Met32 控制人类真菌病原体白色念珠菌中硫代谢灵活性和对活性硫物种的抵抗力的转录调控
• GSE306542 循环细胞群作为转移性肺癌免疫治疗反应预测因子和靶点
• GSE294696 RBMXL3 驱动人类 TCam-2 细胞的转录组变化（RNA-seq 数据）
• GSE293763 支链氨基酸代谢受损通过降低组蛋白H3K23丙酰化加剧血管钙化
• GSE326823 Ca2+通过内质网-质膜接触的内流是棕色脂肪产热和代谢健康所必需的
• GSE322507 MELK抑制通过ERK-cofilin信号通路促进免疫性血小板减少症中的巨核细胞生成和血小板恢复
• GSE313489 CTD 的“拉链”语法控制 RNA 聚合酶 II 转录周期的空间编程 [TT-seq]
• GSE303494 病毒糖蛋白的表达诱导组成型干扰素-β水平升高和CD4 T细胞稳态改变
• GSE302701 对 59 名芬兰参与者的人类皮下脂肪组织活检进行单核 RNA 测序。
• GSE299968 AATF 通过 NRF-1 依赖机制维持线粒体呼吸，从而支持胶质母细胞瘤细胞的增殖 [RNA-seq]
• GSE299967 AATF 通过 NRF-1 依赖性机制维持线粒体呼吸，从而支持胶质母细胞瘤细胞的增殖 [ChIP-seq]
• GSE298536 Dahl盐敏感性大鼠肾脏微血管肌源性张力的维持：C-C基序趋化因子配体2的作用
• GSE293545 CUX1 在控制脂肪细胞分化中表现出物种依赖性调控 [RNA-Seq]
• GSE293544 CUX1 在控制脂肪细胞分化中表现出物种依赖性调控 [ChIP-Seq]
• GSE326425 醛固酮诱导的大脑基因表达变化 [scRNA-Seq]
• GSE326372 改进NOA诊断：通过免疫染色亚分类和Sertoli细胞代谢标志物的双重方法
• GSE326276 唑类化合物C7抑制炎症和衰老可促进造血干细胞和祖细胞的扩增
• GSE326227 失控转录驱动细菌基因中普遍存在的嘌呤偏好
• GSE304478 铜对调节性 T 细胞抑制能力的控制 [RNA-Seq]
• GSE293730 人类牙周膜干细胞在GM/OM和BMP2/BMP9处理下第1、7和15天的转录组分析