详细内容（全部10条）

1. ⭐ GSE325843 Immune-remodeling mRNAs expressing IRF8 or NIK generate durable anti-tumor immunity in multiple cancer models

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immune、immunity
• 📝 描述：Contributors : Akash Gupta ; Riddha Das ; Kaelan Reed ; Taewon Jeon ; Quang Trung Chinh Nguyen ; Arnab Rudra ; Xinying Ge ; Suthathip Trongjit ; Yann S Vanrobaeys ; Robert Langer ; Ralph Weissleder ; Christopher Garris ; Daniel G AndersonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAlthough immunotherapy has a subset of cancer patients, its broader efficacy remains limited, primarily due to an immunosuppressive tumor microenvironment characterized by insufficient numbers of functional tumor-specific T cells, antigen-presenting cells (APCs) and tumor-infiltrating lymphocytes. Here we engineer immune cells in the tumor microenvironment using lipid nanoparticles (LNPs) to deliver immune remodeling mRNAs (IR-mRNAs) encoding NF-κB inducing kinase (NIK) or interferon regulatory factor 8 (IRF8). These IR-mRNAs activate APCs in tumors, significantly increasing activated type 1 conventional dendritic cells, immunostimulatory cytokines, and priming anti-tumor CD8+ T cells. IR-mRNAs encapsulated in LNPs elicited durable antitumor responses in multiple syngeneic mouse tumor models via both intratumoral and intravenous delivery. Co-administration of IR- and OVA-mRNA elicited ~10-fold increase of antigen-specific CD8⁺ T cell responses, sustained long-term memory, and effectively prevented tumor growth in vaccinated mice. Additionally, adjuvanting influenza vaccines with IR-mRNAs enhanced the humoral response by 5-fold and the cellular response by ~15-fold, underscoring their potential as adjuvants for boosting adaptive immunity.
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2. GSE312614 Gαq 缺失重塑成纤维细胞特性并驱动口腔癌进展中的肿瘤-基质重塑

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer
• 📝 描述：Contributors : Inmaculada Navarro-Lérida ; Raquel Huertas-Lárez ; Ramón García-Escudero ; Federico Mayor Jr ; Catalina RibasSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHead and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with limited therapeutic options and poor outcomes due to metastasis. A major driver of tumor progression is the tumor microenvironment, particularly cancer-associated fibroblasts (CAFs), which remodel the extracellular matrix and secrete pro-tumorigenic signals. Emerging evidence suggests that autophagy and vesicle trafficking pathways regulate these secretory functions, though their role in HNSCC remains unclear. Building on our discovery of Gαq as a central autophagy regulator, we investigated how its absence reshapes fibroblast behavior and modulates crosstalk with oral HNSCC cells. Our findings demonstrate that loss of Gαq reprograms murine fibroblasts into a CAF-like phenotype, in part through deregulated intracellular trafficking and increased ceramide accumulation. Gαq-deficient fibroblasts display increased collagen I deposition, ECM remodeling, and a shift toward secretory activity. Their exosomes, enriched in tumor-promoting growth factor receptors, suppress Caveolin 1 expression in tumor cells and induce an EMT-like phenotype that fuels aberrant HNSCC growth. In co-culture and in vivo, Gαq-silenced fibroblasts assemble into “railroad-track” structures that guide cancer cell migration and invasion. Reduced Gαq expression in human fibroblasts recapitulate these features underscoring a conserved mechanism of stromal activation. Overall, Gαq emerges as a key regulator of fibroblast plasticity and tumor-stroma interactions in HNSCC progression.
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3. GSE325923 肿瘤内注射 CD40 激动剂 sotigalimab 与 pembrolizumab 联合治疗转移性黑色素瘤可快速激活抗原呈递细胞，并在非注射肿瘤中驱动抗肿瘤反应：一项 1/2 期研究的结果

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、antigen
• 📝 描述：Contributors : Jingjing Liu ; Jianhua Zhang ; Barbara Pazdrak ; Heather Sonnemann ; Adi Diab ; Gregory LizeeSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCheckpoint inhibitors (CPIs) benefit metastatic melanoma (MM) patients, but resistance remains a challenge. This phase 1/2 (NCT02706353) study evaluated intratumoral sotigalimab (an anti-CD40 agonistic antibody) with pembrolizumab in 32 CPI-naïve MM patients. Primary endpoints included determining safety and tolerability, the recommended phase 2 dose (RP2D) of sotigalimab, and the objective response rate (ORR). The most common adverse events (AEs) related to sotigalimab were injection-site reactions, pruritus, and fatigue. The ORR was 47% and the disease control rate (DCR) was 91% for all patients, and 50% and 92% at the RP2D, respectively. Multiomic biomarker analyses of tumor and blood samples collected before and during treatment demonstrated that sotigalimab effectively engaged the CD40 pathway, boosting the infiltration and activation of myeloid cells, including CD11c+DC-LAMP+ dendritic cells (DCs) and macrophages. The combination therapy activated both innate and adaptive immunity, in both injected and non-injected tumors. TCR sequencing analysis showed increased T-cell clonality with expanded new clones shared between injected and non-injected tumors. Clinical responses correlated with these immunologic changes, but not with baseline immunological features associated with response to anti-PD1 monotherapy. In the B16 melanoma mouse model, single- cell RNA sequencing analysis showed that the combination therapy enhanced antitumor immunity and reduced the immunosuppressive tumor environment. These findings suggest that intratumoral sotigalimab may enhance anti-PD1 therapy, supporting the need for further randomized phase 2 trials to better evaluate its therapeutic potential in the ‘in situ’ immunization approach.
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4. GSE325783 酮体生成通过抑制白血病干细胞中的铁死亡促进白血病发展 [ATAC-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、ATAC-seq
• 📝 描述：Contributors : Xue Han ; Kexin Wang ; Weiwei Ma ; Songqi Zhu ; Jingjing Guan ; Xiaobin Tian ; Zhuangzhhuang Zhao ; Linjia JiangSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusHepatic ketogenesis generates ketone bodies as an alternative energy source during carbohydrate restriction or ketogenic diets, yet its role in non-hepatic cell types remains poorly defined. Here, we show that leukemic stem cells (LSCs) in acute myeloid leukemia (AML) engage elevated ketogenesis, driven by fatty acid oxidation (FAO), to produce D-β-hydroxybutyrate (BHB). LSCs express high levels of 3-hydroxymethylglutaryl-CoA synthase 2 (HMGCS2), the rate-limiting enzyme in ketogenesis than blast cells and normal hematopoietic stem cells (HSCs). Deletion of Hmgcs2 in AML cells markedly decreases BHB levels, disrupts LSC function, and impairs leukemia progression in both mouse and human AML models, while largely sparing normal hematopoiesis. Mechanistically, BHB suppresses ferroptosis by limiting pro-ferroptotic phospholipid remodeling through epigenetic regulation of fatty acid desaturase 2 (FADS2). Together, these findings identify autonomous ketogenesis as a critical metabolic program that protects LSCs from ferroptotic cell death and sustains leukemia progression.
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5. GSE325782 酮体生成通过抑制白血病干细胞中的铁死亡促进白血病发展 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、RNA-seq
• 📝 描述：Contributors : Xue Han ; Kexin Wang ; Weiwei Ma ; Songqi Zhu ; Jingjing Guan ; Xiaobin Tian ; Zhuangzhhuang Zhao ; Linjia JiangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHepatic ketogenesis generates ketone bodies as an alternative energy source during carbohydrate restriction or ketogenic diets, yet its role in non-hepatic cell types remains poorly defined. Here, we show that leukemic stem cells (LSCs) in acute myeloid leukemia (AML) engage elevated ketogenesis, driven by fatty acid oxidation (FAO), to produce D-β-hydroxybutyrate (BHB). LSCs express high levels of 3-hydroxymethylglutaryl-CoA synthase 2 (HMGCS2), the rate-limiting enzyme in ketogenesis than blast cells and normal hematopoietic stem cells (HSCs). Deletion of Hmgcs2 in AML cells markedly decreases BHB levels, disrupts LSC function, and impairs leukemia progression in both mouse and human AML models, while largely sparing normal hematopoiesis. Mechanistically, BHB suppresses ferroptosis by limiting pro-ferroptotic phospholipid remodeling through epigenetic regulation of fatty acid desaturase 2 (FADS2). Together, these findings identify autonomous ketogenesis as a critical metabolic program that protects LSCs from ferroptotic cell death and sustains leukemia progression.
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6. GSE213430 ASCL1 在神经母细胞瘤细胞系中过表达 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：Contributors : Lidiya Mykhaylechko ; Laura M Woods ; Anna PhilpottSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo compare the function of overexpressed ASCL1 in two MYCN-amplified neuroblastoma cell contexts, we have used a doxycycline-inducible lentivirus system to induce ASCL1 overexpression in SK-N-BE(2)C and IMR-32 cell lines, and assessed the transcriptome wide impact of ASCL1 overexpression using RNA-seq.
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7. GSE213247 ASCL1 在神经母细胞瘤细胞系中的染色质结合 [ChIP-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：ChIP-seq
• 📝 描述：Contributors : Lidiya Mykhaylechko ; Laura M Woods ; Anna PhilpottSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensTo compare the ASCL1 protein binding to chromatin in two MYCN-amplified neuroblastoma cell contexts, we have used a doxycycline-inducible lentivirus system to induce ASCL1 overexpression in SK-N-BE(2)C and IMR-32 cell lines, and assessed ASCL1 binding using ChIP-seq.
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8. GSE326035 叶面喷施铁剂可延缓水稻灌浆后期类黄酮的降解，并调节有色稻米的糖代谢。

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Oryza sativaIntroduction Colored rice is recognized for nutritious function due to its flavonoid compounds. Flavonoid aglycones are conjugated with various sugar moieties. Iron (Fe) is an essential mineral nutrient for plants; however, the regulatory mechanisms by which Fe influences flavonoid accumulation dynamics and sugar metabolism remain unclear. Methods FeSO4 was foliar-applied to black rice cultivar Nanheinuo and red rice cultivar Yuhongdao 5815 to study regulatory mechanisms by which Fe influences flavonoid accumulation dynamics and sugar metabolism Results and discussion The content of anthocyanins, proanthocyanidins, and total flavonoids in the grains decreased throughout the later filling stages. Fe application delayed their degradation during this phase. The differential expression of numerous genes associated with flavonoid transformation may be the primary reason. Genes annotated as key enzymes involved in phenylpropanoid and flavonoid biosynthesis were significantly upregulated in Nanheinuo. Additionally, foliar Fe application significantly impacted sugar and sugar alcohol content by regulating the expression of genes involved in polysaccharide decomposition, phosphorylation and dephosphorylation processes, and sugar interconversion. A close relationship was observed between sugar content and flavonoid-related compound content in the grains of colored rice. Multiple hub genes annotated as glycoside hydrolases in KEGG occupied central nodes within the co-expression network, suggesting that glycoside hydrolases may mediate the crosstalk between sugar and flavonoid metabolism in colored rice grains under Fe treatment, although glycosyltransferases primarily function in flavonoid glycosylation. Foliar Fe application delays flavonoid degradation during the later filling stage and regulates sugar metabolism in colored rice, thereby influencing flavonoid enrichment and eating quality.
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9. GSE325004 抗逆转录病毒疗法抑制免疫缺陷病毒感染期间免疫系统编程的不同阶段

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Macaca mulattaThis project contains rhesus macaque immune cell single-cell RNA-seq data, from SIV/ART studies.
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10. GSE278295 过表达 PAI-1 的 CAF 与 Panc-1 细胞共培养的 RNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Contributors : Xuan Zhou ; Yu Ren ; Jihui HaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the effect of CAF overexpressing PAI-1 on tumor cells, CAF overexpressing PAI-1 was used to co-culture with Panc-1 cells, and then gene expression profiling of Panc-1 cells was performed using RNA-seq.
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详细内容（全部1条）

1. 世界上最小的二维码比细菌还小，可以存储数据数百年。

• ✍️ 作者：未知作者
• 🏷️ 关键词：bacteria、regex:bacter(ia|ial|ium)
• 📝 描述：Scientists have created a microscopic QR code so tiny it can only be seen with an electron microscope—smaller than most bacteria and now officially a world record. But this isn’t just about size; it’s about durability. By engraving data into ultra-stable ceramic materials, the team has opened the door to storing information that could last for centuries or even millennia without needing power or maintenance.
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