详细内容（前10条）

1. ⭐ GSE308302 雷帕霉素治疗可改善 HLA-B27 介导的肠道炎症并改变实验性脊柱关节炎的微生物群

• ✍️ 作者：未知作者
• 🏷️ 关键词：inflammation、HLA、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Jinny Van Doorn ; Stephen R Brooks ; Francesca LiCausi ; Kelly Zhou ; Naga S Betrapally ; Eva Gubitz-Hess ; Antony Cougnoux ; Stefania Dell’Orso ; Shamima Islam ; Robert A Colbert ; Fatemeh NavidSeries Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusRapamycin treatment reduced stool and colon histological scores in B27-Tg rats compared to vehicle-treated B27-Tg controls. Transcriptome analysis revealed that rapamycin reduced expression of key pro-inflammatory cytokines like Il17a, Il17f, Tnf, Il1a, IL1b, and Il22 in B27-Tg colon tissue compared to vehicle-treated B27-Tg controls. Ex vivo treatment of bulk immune cells isolated from B27-Tg rat colon with rapamycin (25 and 50 nM) reduced expression of Il17a, Il17f, Ifng, and Il22 compared to vehicle-treated cells. Rapamycin treatment decreased the abundance of cecum microbiota associated with inflammation in B27-Tg rats. Rapamycin also altered the gut microbiome in WT rats, without associated changes in the tissue transcriptome. Our study demonstrates that rapamycin treatment substantially reduces HLA-B27-mediated gut inflammation in experimental SpA. Results from this pre-clinical model suggest further evaluation of rapamycin as a therapeutic strategy in HLA-B27 associated diseases is warranted.
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2. ⭐ GSE303880 研究发现，林奇综合征中 MAIT 细胞的富集与免疫监视和结直肠癌风险相关。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune、enrichment
• 📝 描述：Contributors : Hairu Yang ; Ken Cadwell ; Meenakshi Bewtra ; Bryson W KatonaSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensTissue microenvironment characteristics associated with elevated risk of colorectal cancer (CRC) in Lynch syndrome (LS) are poorly characterized. We applied the multimodal single cell sequencing platform ExCITE-seq to define the colonic cellular composition and transcriptome of LS carriers with and without a history of CRC compared with general population controls. Our analysis revealed widespread remodeling in LS that included striking expansion of epithelial stem and progenitor cells, and loss of fibroblast populations. Although clonally expanded and terminally exhausted CD8 T cells were more prominent in individuals with a history of CRC, LS carriers without CRC displayed enrichment of cytotoxic mucosal-associated invariant T (MAIT) cells associated with CCL20 expression in epithelial progenitors, validated by orthogonal techniques including demonstration of a protective function in a murine model of CRC. These findings highlight cellular features that distinguish LS carriers and suggest a protective role of MAIT cells in human CRC surveillance.
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3. ⭐ 利用GSE324478 RNA测序分析Col-0和rpt2a-2在局部感染和系统获得对丁香假单胞菌斑点致病变种ES4362的抗性过程中转录组的全局变化

• ✍️ 作者：未知作者
• 🏷️ 关键词：resistance、sequencing、transcriptome
• 📝 描述：Contributors : Gautier Langin ; Suayb Üstün ; Daniela Spinti ; Frederik BörnkeSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaThe ubiquitin-proteasome-system (UPS) is a cellular cascade involving three enzymatic steps for protein ubiquitination to target them to the 26S proteasome for proteolytic degradation. Several components of the UPS have been shown to be central for regulation of defense responses during infections with phytopathogenic bacteria. Upon recognition of the pathogen, local defense is induced which also primes the plant to acquire systemic resistance (SAR) for enhanced immune responses upon challenging infections. In this experiment we analyzed the immune response of Col-0 and rpt2a-2 mutant to the pathogenic bacteria Pseudomonas syringae pv. maculicola ES4362 at the site of infection or during SAR.
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4. ⭐ GSE292222 巨噬细胞内在和IL-9依赖的精氨酸代谢促进肺肿瘤生长

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、macrophage、metabolism
• 📝 描述：Contributors : Anthony M Cannon ; James Ropa ; Mark H KaplanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTumor-associated macrophages are an abundant, tumor-infiltrating cell population that supports the evasion of tumor cells from anti-tumoral immune cell detection by generating an immunosuppressive tumor-immune microenvironment (TIME). The immunosuppressive function of macrophages is dictated by the cytokine environment. IL-9 is a pleiotropic cytokine that can be a positive or negative regulator of tumor growth. Our lab previously identified a pro-tumoral role of IL-9 by expanding lung interstitial macrophage populations and inducing the expression of arginase 1 (ARG1) to enhance tumor growth. However, the underlying mechanism by which IL-9R/ARG1+ interstitial macrophages promote tumor progression remains incomplete. Here, we demonstrate that macrophage-targeting nanoparticles containing Arg1 siRNA can therapeutically reduce tumor burden and reduce pro-tumor arginine-derived metabolite production. Furthermore, using bulk RNA sequencing of lung macrophages isolated from Il9r-/-:WT mixed-bone marrow chimeric mice, we demonstrate that IL-9 intrinsically alters the transcriptomic landscape of lung interstitial macrophages. Mechanistically, IL-9 promotes intrinsic Arg1 expression through an IRF4-dependent regulatory pathway and modulates arginine and polyamine concentration within interstitial macrophages and lung tissue, resulting in increased lung tumor growth and altered macrophage phenotypes. Thus, our work defines a pro-tumor mechanism of IL-9-responsive macrophages mediated by altered intrinsic arginine metabolism in lung interstitial macrophages that enhances lung tumor growth.
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5. ⭐ GSE324569 γ疱疹病毒通过骨髓微环境中的 CD8⁺ T 细胞-单核细胞对话重编程长期髓系造血 [scRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：T cell、monocyte、scRNA
• 📝 描述：Contributors : Arthur Poncelet ; Olivier Botman ; Abdulkader Azouz ; Aurélie Detavernier ; Vincent Martens ; Muriel Nguyen ; Séverine Thomas ; Valérie Acolty ; Adeline Rosu ; Céline Maquet ; Lucia Rodriguez-Rodriguez ; Rémy Sandor ; Justine Javaux ; Malyvanh Pathammavong ; Christian Münz ; Anthony Rongvaux ; Laurent Gillet ; Stanislas Goriely ; Bénédicte MachielsSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusThe immune system is continuously shaped by microorganisms, with viruses emerging as key drivers of immune variability. Gammaherpesviruses (γHVs), including Epstein–Barr virus, have coevolved with their hosts to establish lifelong infections, and may imprint durable innate immune memory. Using Murid herpesvirus 4 in wild-type mice and EBV in humanized models, we show that γHVs remodel monocytes and hematopoietic stem cells at phenotypic, transcriptomic, and epigenetic levels, resulting in altered responses to heterologous challenge. Viral persistence is required for immune priming, and IFN-γ signaling directly reprograms myelopoiesis. During latency, virus-specific resident CD8⁺ T cells accumulate in the bone marrow, and remain poised for rapid IFN-γ release. Upon low-dose LPS stimulation, monocyte-derived IL-27, IL-18, and IL-12 promote bystander IFN-γ secretion from these CD8+ T cells, amplifying myelopoietic rewiring. Thus, γHV latency establishes a cytokine circuit that shapes innate immune training and defines a new equilibrium between host and persistent viruses.
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6. ⭐ GSE324551 γ疱疹病毒通过骨髓微环境中的 CD8⁺ T 细胞-单核细胞对话重编程长期髓系造血 [scATAC-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：T cell、monocyte、scATAC
• 📝 描述：Contributors : Arthur Poncelet ; Olivier Botman ; Abdulkader Azouz ; Aurélie Detavernier ; Vincent Martens ; Muriel Nguyen ; Séverine Thomas ; Valérie Acolty ; Adeline Rosu ; Céline Maquet ; Lucia Rodriguez-Rodriguez ; Rémy Sandor ; Justine Javaux ; Malyvanh Pathammavong ; Christian Münz ; Anthony Rongvaux ; Laurent Gillet ; Stanislas Goriely ; Bénédicte MachielsSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThe immune system is continuously shaped by microorganisms, with viruses emerging as key drivers of immune variability. Gammaherpesviruses (γHVs), including Epstein–Barr virus, have coevolved with their hosts to establish lifelong infections, and may imprint durable innate immune memory. Using Murid herpesvirus 4 in wild-type mice and EBV in humanized models, we show that γHVs remodel monocytes and hematopoietic stem cells at phenotypic, transcriptomic, and epigenetic levels, resulting in altered responses to heterologous challenge. Viral persistence is required for immune priming, and IFN-γ signaling directly reprograms myelopoiesis. During latency, virus-specific resident CD8⁺ T cells accumulate in the bone marrow, and remain poised for rapid IFN-γ release. Upon low-dose LPS stimulation, monocyte-derived IL-27, IL-18, and IL-12 promote bystander IFN-γ secretion from these CD8+ T cells, amplifying myelopoietic rewiring. Thus, γHV latency establishes a cytokine circuit that shapes innate immune training and defines a new equilibrium between host and persistent viruses.
• 🔗 查看原文

7. ⭐ GSE324548 γ疱疹病毒通过骨髓微环境中的 CD8⁺ T 细胞-单核细胞对话重编程长期髓系造血 [bulk ATAC-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：T cell、monocyte、ATAC-seq
• 📝 描述：Contributors : Arthur Poncelet ; Olivier Botman ; Abdulkader Azouz ; Aurélie Detavernier ; Vincent Martens ; Muriel Nguyen ; Séverine Thomas ; Valérie Acolty ; Adeline Rosu ; Céline Maquet ; Lucia Rodriguez-Rodriguez ; Rémy Sandor ; Justine Javaux ; Malyvanh Pathammavong ; Christian Münz ; Anthony Rongvaux ; Laurent Gillet ; Stanislas Goriely ; Bénédicte MachielsSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThe immune system is continuously shaped by microorganisms, with viruses emerging as key drivers of immune variability. Gammaherpesviruses (γHVs), including Epstein–Barr virus, have coevolved with their hosts to establish lifelong infections, and may imprint durable innate immune memory. Using Murid herpesvirus 4 in wild-type mice and EBV in humanized models, we show that γHVs remodel monocytes and hematopoietic stem cells at phenotypic, transcriptomic, and epigenetic levels, resulting in altered responses to heterologous challenge. Viral persistence is required for immune priming, and IFN-γ signaling directly reprograms myelopoiesis. During latency, virus-specific resident CD8⁺ T cells accumulate in the bone marrow, and remain poised for rapid IFN-γ release. Upon low-dose LPS stimulation, monocyte-derived IL-27, IL-18, and IL-12 promote bystander IFN-γ secretion from these CD8+ T cells, amplifying myelopoietic rewiring. Thus, γHV latency establishes a cytokine circuit that shapes innate immune training and defines a new equilibrium between host and persistent viruses.
• 🔗 查看原文

8. ⭐ GSE270765 结直肠癌播散性肿瘤细胞采用表皮角质形成细胞的伤口愈合程序 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、RNA-seq
• 📝 描述：Contributor : Ryoji YaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensDisseminated tumor cells (DTCs) are a critical cell population in the metastasis process, and understanding their features is essential for the development of cancer treatment. We show that DTCs of colorectal cancer (CRC) transiently adopted the cellular state resembling to the activated epidermal keratinocyte during wound healing. Using the orthotropic transplantation of patient derived organoids (PDOs), we demonstrated that collective migrating cell clusters in primary site did not contain cancer stem cells but contained cells expressing wound-inducible keratins. Mechanistically, inhibition of EZH2 methyltransferase and activation of YAP signaling synergistically promoted DTC phenotype and potentiated dissemination to the distant organs. Collectively, these observations demonstrated that CRC cells alter the cellular hierarchy and adopt re-epithelization program resembling to the tissue repair process to generate DTCs.
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9. GSE324454 解析乳腺癌脑转移的细胞结构揭示了预后不同的免疫图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
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10. GSE317339 EBV感染结果由单核细胞和Treg驱动的免疫动力学在体外PBMC模型中决定

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、monocyte
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
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详细内容（全部2条）

1. 一项开放的单细胞RNA测序图谱揭示了海胆发育过程中的神经分化动态

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、single-cell
• 📝 描述：RNA sequencing at single-cell resolution reveals developmental cell states in sea urchin embryos, helping researchers trace neurogenesis and explore gene expression…
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2. 科学家注射一个肿瘤细胞，然后观察体内其他部位的癌细胞消失。

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer
• 📝 描述：A redesigned cancer immunotherapy is showing striking early results after decades of disappointment with similar drugs. Researchers engineered a more powerful CD40 agonist antibody and changed how it’s delivered—injecting it directly into tumors instead of into the bloodstream. In a small clinical trial of 12 patients with metastatic cancers, six saw their tumors shrink and two experienced complete remission.
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• GSE305323 Gnmt 过表达对果蝇感光细胞组蛋白甲基化丰度的影响
• GSE266630 小鼠 DNA 甲基化模式的非孟德尔遗传广泛存在 [RNA-Seq 肝脏]
• GSE324697 毛里求斯猕猴和印度猕猴外周血单核细胞中等位基因特异性MHC表达的单细胞RNA分析
• GSE300005 独特的序列特征定义了炎症记忆的表观遗传持久性 [RNA-seq]
• GSE300003 独特的序列特征定义了炎症记忆的表观遗传持久性 [ChIP-Seq]
• GSE299457 独特的序列特征定义了炎症记忆的表观遗传持久性 [scRNA-seq]
• GSE299177 独特的序列特征定义了炎症记忆的表观遗传持久性 [ATAC-seq]
• GSE140820 RNA-Seq 数据，用于分析 PyMT 乳腺肿瘤细胞（LSD1 缺失或未缺失）的 RNA-Seq 情况。
• 利用GSE324480 RNA测序分析Col-0或nac53-1 78-1中对番茄假单胞菌DC3000的免疫反应
• GSE324641 γ疱疹病毒通过骨髓微环境中的CD8⁺ T细胞-单核细胞对话重编程长期髓系造血
• GSE324568 γ疱疹病毒通过骨髓微环境中的 CD8⁺ T 细胞-单核细胞对话重编程长期髓系造血 [scMultiome]
• GSE305481 基因组规模代谢建模揭示与 APOE 变异相关的细胞类型特异性特征
• GSE324958 细胞跨物种RNA测序图谱揭示了肌生成的转录动态
• GSE296494 小鼠 DNA 甲基化模式的非孟德尔遗传广泛存在 [ONT 肌肉]
• GSE293012 异基因造血干细胞移植后患者骨髓细胞的单细胞RNA测序
• GSE266632 小鼠 DNA 甲基化模式的非孟德尔遗传广泛存在 [ONT 肝脏 - 靶向]
• GSE266631 小鼠 DNA 甲基化模式的非孟德尔遗传广泛存在 [ONT 肝脏]
• GSE324852 雌性小鼠在受孕前暴露于一系列代谢干扰物会导致其后代出现性别二态性代谢紊乱
• GSE324619 欧洲和非洲人群单核细胞甲基化组遗传效应图谱
• GSE316832 中重度斑秃患者头皮的全面单细胞转录组分析
• GSE308071 颅内间叶肿瘤，FET-CREB融合阳性：81例病例的综合分析
• GSE299606 独特的序列特征定义了炎症记忆的表观遗传持久性 [亚硫酸氢盐测序]
• GSE299178 独特的序列特征定义了炎症记忆的表观遗传持久性 [CUT&Run]
• GSE324703 APOBEC3B 通过诱导 R 环形成和基因组不稳定性来调控 HPV 复制 [RNA-Seq]
• GSE324673 利用人诱导多能干细胞无饲养层生成淋巴内皮细胞
• GSE324516 APOBEC3B 通过诱导 R 环形成和基因组不稳定性来调控 HPV 复制 [ChIP-Seq]
• GSE324384 LCN2缺陷型PC-3细胞的功能和基因组特征揭示了前列腺癌进展的见解
• GSE319077 CDKL5 缺乏症患者来源的类器官中的兴奋性皮层神经元表现出神经源性2 诱导的神经元中未发现的早期过度兴奋性 [RNA-Seq]
• GSE291054 POLR1F 通过激活 F2R/p38 MAPK 信号通路促进未分化型甲状腺癌的增殖和干性
• GSE316090 揭示 BIN2-GRF5-UBP12/13 模块：低硝酸盐条件下根系觅食的新型调控途径
• GSE310764 绝经后卵巢从生殖器官转变为免疫器官
• GSE303157 Lcn2 缺陷导致社交障碍，且与母体免疫激活无关
• GSE295427 靶向支链氨基酸可缓解肺纤维化 [scRNA-seq]
• GSE295426 靶向支链氨基酸可缓解肺纤维化 [RNA-seq]
• GSE295423 靶向支链氨基酸可缓解肺纤维化 [ATAC-seq]