详细内容（前10条）

1. ⭐ GSE323136 单细胞 RNA 测序通过减轻个体间取样异质性，揭示了囊性纤维化患者持续存在的上皮和免疫功能障碍

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、RNAseq、single-cell
• 📝 描述：Contributors : Thaddeus Cybulski ; Marc A Sala ; Trevor Nicholson ; Zasu Klug ; Rachel Nelson ; Zhan Yu ; Yuliana Sokolenko ; Estefani Diaz ; Suchitra Swaminathan ; Ziyan Lu ; Nikolay S Markov ; Edward F McKone ; Alexander V Misharin ; Manu JainSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensNasal epithelium (NE) is used as a surrogate for lower airway epithelium in cystic fibrosis (CF). However, NE is composed of diverse cell types with unique functions; changes to this heterogeneous tissue in disease can be cell-type specific. To explore the impact of tissue heterogeneity, we examine NE gene expression and cell-type heterogeneity using single-cell transcriptomics of people with CF (PwCF) and healthy volunteers (HV). We also compare cell-type abundance in paired single-cell and bulk transcriptomic samples.
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2. ⭐ GSE322943 剖析乳腺癌脑转移的细胞结构揭示了预后不同的免疫图谱 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune、RNA-seq
• 📝 描述：Contributors : Lena Jassowicz ; Rolf Warta ; Christel Herold-MendeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBreast cancer brain metastases (BCBM) are a severe condition with high demand for improved personalized treatment, but a comprehensive understanding of BCBM immune-microenvironment heterogeneity and susceptibility to immunotherapy is lacking. Here, we multimodally profile the immune niche in a vast, clinically well-annotated cohort of 156 BCBM applying tissue cytometry, bulk and single nuclei RNA-sequencing, flow cytometry, and spatial transcriptomics, complemented by functional studies in patient-derived models. Integrative analyses reveal two immune landscapes predicting prolonged patient survival and not deducible from paired primary tumors: 1) BCBM with a high proportion of CD8 tissue-resident-like memory T cells as major players of tumor immune control. 2) BCBM containing tertiary lymphoid structures. Surrogate signatures of these landscapes are prognostic in independent BCBM and primary breast cancer cohorts, are associated with fewer metastases, and predict immunotherapy response. Our work provides critical insights into anti-tumor immunity in BCBM and identifies novel biomarkers with translational relevance.
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3. GSE310839 利用抑制剂 JQKD82 对 KDM5A/B 进行药理学抑制的 B 细胞淋巴瘤细胞进行转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：lymphoma、transcriptome
• 📝 描述：Contributors : Dawei Zhou ; Zhenyu Wu ; Jian ZhuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHistone methylation critically controls the progression of human diseases, including infections and cancers. Our study reported that histone lysine demethylases (KDMs) in the KDM5 family KDM5A/B play profound roles in suppressing lytic reactivation of oncogenic human herpesvirus 8 (HHV-8), i.e., Kaposi’s sarcoma-associated herpesvirus (KSHV), a human gamma-herpesvirus and an etiological agent of multiple malignancies. During KSHV lytic reactivation from latency, host innate immune responses are also activated, which is expected to favor the tumor cell killing by generating the additional oncolytic effects. To further study the systemic effect of KSHV on host immune response under the regulation of KDM5A/B, we performed the transcriptome profiling analysis for both KSHV-infected BCBL-1 vs KSHV-negative BJAB cells with or without JQKD82 treatment by RNA-seq. Our RNA-seq analysis and further experimental validation suggested that the KDM5 inhibitor JQKD82 is capable of inducing antiviral/antitumor innate immune responses in KSHV-negative B-cell lymphomas, but such effects are more striking in KSHV-positive B-cell lymphomas likely due to KSHV lytic reactivation that further triggers the innate immune activation in tumor cells.
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4. GSE138625 利用单细胞 RNA 测序 (Chromium 10x) 分析果蝇三龄幼虫翅盘的单细胞转录谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、10x
• 📝 描述：Contributors : Maria P Zappia ; Maxim V Frolov ; Abul B IslamSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila melanogasterOur study focuses on understanding the early transcriptional changes taking place during the divergence of the adult muscle precursors that give rise to indirect flight muscles and direct flight muscles in Drosophila. Here, we studied the functional relevance of Arginine Kinase in flight muscle development by depleting Argk expression specifically in the adult muscle precursors. We determined the transcriptional changes and perturbations in AMP cell identity upon Argk knockdown.
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5. GSE324453 剖析乳腺癌脑转移的细胞结构揭示了预后不同的免疫图谱 [snRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune
• 📝 描述：Contributors : Lena Jassowicz ; Fangyoumin Feng ; Rolf Warta ; Marc Zapatka ; Martina Seiffert ; Christel Herold-MendeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBreast cancer brain metastases (BCBM) are a severe condition with high demand for improved personalized treatment, but a comprehensive understanding of BCBM immune-microenvironment heterogeneity and susceptibility to immunotherapy is lacking. Here, we multimodally profile the immune niche in a vast, clinically well-annotated cohort of 156 BCBM applying tissue cytometry, bulk and single nuclei RNA-sequencing, flow cytometry, and spatial transcriptomics, complemented by functional studies in patient-derived models. Integrative analyses reveal two immune landscapes predicting prolonged patient survival and not deducible from paired primary tumors: 1) BCBM with a high proportion of CD8 tissue-resident-like memory T cells as major players of tumor immune control. 2) BCBM containing tertiary lymphoid structures. Surrogate signatures of these landscapes are prognostic in independent BCBM and primary breast cancer cohorts, are associated with fewer metastases, and predict immunotherapy response. Our work provides critical insights into anti-tumor immunity in BCBM and identifies novel biomarkers with translational relevance.
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6. GSE317798 分选连接蛋白 5 是 MHC II 类抗原呈递和宿主防御结核分枝杆菌感染所必需的。

• ✍️ 作者：未知作者
• 🏷️ 关键词：antigen、MHC
• 📝 描述：Contributors : Kubra Naqvi ; Victoria Ektnitphong ; Samuel Alvares-Arguedas ; Kathryn Rahlwes ; Priscila Campos ; Michael ShilohSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTuberculosis (TB) remains one of the leading causes of death from a single infectious agent worldwide, yet the host pathways that regulate antigen presentation and lung inflammation during Mycobacterium tuberculosis (Mtb) infection are incompletely defined. Sorting nexin 5 (SNX5) is a phox homology (PX) and bin, amphiphysin, rvs (BAR) domain protein best known for roles in endosomal trafficking, antigen processing, and antiviral host defense, but its contribution to immunity during Mtb infection is unknown. Here, we show that SNX5-deficient mice exhibit markedly increased mortality following low-dose aerosol infection despite unchanged pulmonary bacterial burden compared to wild-type mice. Snx5-/- mice developed exacerbated lung inflammation without major alterations in immune cell recruitment. In macrophages, Snx5-/- did not affect phagocytosis, vacuolar maturation, intracellular bacterial control, or global transcriptional responses to Mtb, but was required for efficient MHC class II antigen presentation. Snx5 deficiency reduced antigen degradation, limited peptide loading onto MHC-II and impaired activation of antigen-specific CD4+ T cells without altering surface MHC-II abundance or expression of costimulatory molecules. Together, these findings identify SNX5 as a previously unrecognized regulator of MHC-II peptide loading that shapes inflammatory outcomes during pulmonary Mtb infection, highlighting a role for endosomal sorting machinery in immunity to intracellular pathogens.
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7. GSE306615：WERI-Rb1视网膜母细胞瘤细胞转录组分析，阐明ELF2驱动的拓扑替康耐药机制

• ✍️ 作者：未知作者
• 🏷️ 关键词：resistance、transcriptome
• 📝 描述：Contributors : Jing Zhuang ; Guei-Sheung Liu ; Shuilian Chen ; Jingyi JiangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the role of ELF2 in topotecan resistance, we performed RNA-seq on WERI-Rb1 retinoblastoma cells. Control (NTC) and ELF2-knockout (sgELF2) cells were treated with either vehicle or 30 nM topotecan for 96 hours. Differential gene expression analysis revealed transcriptomic changes associated with ELF2 loss and its impact on chemoresistance, providing insights into potential mechanisms and therapeutic targets.
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8. GSE324116 沃勒米鱼甲基化测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、methylation
• 📝 描述：Contributors : Srividya Ramakrishnan ; Cristiane de Santis Alves Rosa ; Lais Araujo Coelho ; Melissa Kramer ; Sara Goodwin ; Olivia Mendivil Ramos ; Gil Eshel ; Veronica M Sondervan ; Samantha Frangos ; Cecilia Zumajo-Cardona ; Katharine M Jenike ; Shujun Ou ; Xiaojin Wang ; Yin Peng Lee ; Stella Loke ; Maurizio Rossetto ; Hannah McPherson ; Sebastiano Nigris ; Silvia Moschin ; Damon P Little ; Manpreet S Katari ; Kranthi Varala ; Sergios-Orestis Kolokotronis ; Barbara A Ambrose ; Larry J Croft ; Gloria M Coruzzi ; Dennis W Stevenson ; Michael C Schatz ; W. Richard McCombie ; Robert A MartienssenSeries Type : Methylation profiling by high throughput sequencingOrganism : Wollemia nobilisWe performed methylation profiling analysis from Bisulfite-seq of 2 different reproductive tissues: ovule and pollen
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9. GSE323357 骨转移微环境的单细胞和空间转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、spatial
• 📝 描述：Contributors : Stephen Wong ; Xiang Zhang ; Jianting Sheng ; Zhan Xu ; Li Yang ; Ju Young Ahn ; Yuliang CaoSeries Type : Other ; Expression profiling by high throughput sequencingOrganism : Mus musculusThe goal of this study is to comprehensively characterize the cellular composition, transcriptional programs, and spatial organization of the bone metastatic microenvironment and to assess how therapeutic perturbation influences tumor–microenvironment interactions. A mouse bone metastasis model was established via internal iliac artery injection of Lewis lung carcinoma cells, and metastatic lesions were monitored by in vivo imaging. Single-cell RNA sequencing and 10x Genomics Visium–based spatial transcriptomics were generated from metastatic bone tissues to provide complementary cellular and spatial resolution.
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10. GSE323351 健康肺永生化 II 型肺泡细胞系的单细胞 RNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Contributors : Anas Rabata ; Dianhua JiangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensUsing the 10x Genomics platform, we conducted single-cell RNA sequencing to profile our newly immortalized alveolar type 2 (AT2) cell lines. Since primary human AT2 cells are notoriously difficult to propagate long-term, passage repeatedly, and modify genetically, we developed several stable, immortalized lines derived from healthy AT2 cells.
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1. patentsview R 包发布

• ✍️ 作者：未知作者
• 🏷️ 关键词：R package
• 📝 描述：The patentsview R package was created by Chris Baker to simplify interactions with the PatentsView API as announced in Chris’ blog post in 2017. The API can be queried for data from US patents granted since 1976 as well as patent applications si… Continue reading: Breaking Release of the patentsview R Package
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1. 利用RNA测序指导的5′UTR设计改进mRNA疗法

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Large scale RNA sequencing and transcriptome analysis helped identify optimized 5′UTR sequences that improve protein production from mRNA therapeutics, supporting vaccine and therapeutic performance in aging and obesity models…
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2. 利用纳米孔直接RNA测序评估mRNA和sgRNA在细胞和基因治疗应用中的质量

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Nanopore RNA sequencing enables detailed analysis of mRNA and sgRNA quality attributes, supporting the development and manufacturing of safer and more effective RNA based therapeutics…
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3. 斯坦福大学科学家表示，色盲可能掩盖致命的膀胱癌预警信号。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Colorblindness may be doing more than making traffic lights confusing — it could also be hiding a life-threatening warning sign. Researchers analyzing millions of medical records found that people with bladder cancer who are also colorblind have a 52% higher mortality rate over 20 years compared to those with normal vision. The likely reason: many people with color vision deficiency struggle to see red, making it harder to notice blood in urine, the most common early sign of bladder cancer.
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4. 研究人员在美国农村地区发现了令人担忧的阿尔茨海默病模式

• ✍️ 作者：未知作者
• 🏷️ 关键词：Alzheimer
• 📝 描述：A new analysis of more than 422,000 patients reveals a stark Alzheimer’s care gap between Maryland’s urban and rural communities. Rural regions often lack hospitals and dementia specialists, forcing older patients to travel farther for diagnosis and treatment. Researchers found areas with high Alzheimer’s death rates but surprisingly low diagnosis rates, suggesting many cases may never be identified.
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5. 人工智能最终验证了一个关于癌症起源的百年理论。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Cancer often begins when the genetic instructions that guide our cells become scrambled, allowing cells to grow uncontrollably. Now, scientists at EMBL have developed an AI-powered system called MAGIC that can automatically spot and tag cells showing early signs of chromosomal trouble—tiny DNA-filled structures known as micronuclei that are linked to future cancer development.
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6. 科学家发现一种能阻止侵袭性乳腺癌发展的分子

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists at Oregon Health & Science University have developed a new molecule that could open the door to treating triple-negative breast cancer, one of the most aggressive and difficult-to-treat forms of the disease. The compound, called SU212, targets and disables a key enzyme that cancer cells rely on to fuel their growth. In tests using humanized mouse models, the molecule caused tumors to shrink and slowed the spread of cancer by forcing the enzyme to break down.
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• GSE319243 脑移植的血液和颅骨骨髓单核细胞衍生巨噬细胞表现出不同的特性 [ATAC-seq]
• GSE324223 SEPHS2 缺陷通过 NAD+ 稳定 PCK1，重编程葡萄糖代谢以激活磷酸戊糖途径。
• GSE324430 MAPT基因座启动子诱变和大规模并行报告基因筛选鉴定顺式调控元件和遗传变异效应[RNA-seq]
• GSE322657 KLF2 抑制驻留记忆 CD8 T 细胞分化程序，这与 KLF3 的作用相反。
• GSE312426 衰老菌落中大肠杆菌分离株的基因表达谱分析（系列 2）
• GSE312419 衰老菌落中大肠杆菌分离株的基因表达谱分析（系列 1）
• GSE312404 利用 uMRT 对合成 RNA 池中 TRMT61B 介导的 m¹A 甲基化进行定位
• GSE299237 DBA/2J 和 DBA/2J-Gpnmb+/SjJ 小鼠虹膜角膜区域的比较 RNA 测序分析
• GSE296176 斑马鱼心脏发育的单细胞转录组分析。
• GSE324288 研究发现，细胞珠蛋白基因缺失会加剧心脏肥大，并抑制心脏成纤维细胞活化，且该过程与血压变化无关。
• GSE324265 细胞毒性 CD8 T 细胞、中性粒细胞和 1 型干扰素信号传导对 HIV 相关结核性脑膜炎过度炎症病理的贡献 [RNA-Seq]
• GSE322955 RNA-seq 数据用于分析不同条件下恶臭假单胞菌 KT2440 的调控网络。
• GSE322719 RNA-seq 数据用于分析不同条件下番茄丁香假单胞菌 DC3000 的调控网络。
• GSE322682 RNA-seq 数据用于分析铜绿假单胞菌 PAO1 在不同条件下的调控网络。
• GSE316567 跨平台心脏组织单核分子和细胞分析特征的综合研究 [10X_snRNAseq]
• GSE316565 跨平台心脏组织单核分子和细胞分析特征的综合研究 [ICELL8_snRNAseq]
• GSE311354 针对含有非洲中心性TP53 P47S变异体的癌症的个性化治疗方法
• GSE310169 4D力模式实现对血管生成的空间控制
• GSE310145 空间转录组分析揭示银屑病皮损中身体部位特异性的炎症差异
• GSE307923 视网膜类器官单细胞转录组学研究揭示了PRPF31突变对穆勒胶质细胞活化和进行性感光细胞退化的影响
• GSE276526 骨髓间充质干细胞单细胞测序
• GSE324412 信息素回路和转录级联通过社会经验调节果蝇中枢脑的转录和染色质状态（RNA测序）
• GSE324217 PGLYRP1在百日咳杆菌感染期间宿主防御和免疫调节中的双重作用
• GSE324200 过氧化物酶体通过细胞器间级联反应协调代谢灵活性和寿命
• GSE324129 异染色质的转录调控是三维基因组接触遗传变异的基础 V
• GSE324043 雷公藤内酯醇通过下调PI3K/Akt/mTOR信号通路诱导人骨肉瘤细胞自噬和凋亡
• GSE324017 沃勒米鱼小RNA测序
• GSE322757 IL-17RC信号通路连接肠道菌群和神经免疫相互作用在动脉粥样硬化中的作用
• GSE320070 脑移植的血液和颅骨骨髓单核细胞衍生巨噬细胞表现出不同的特性 [scRNA_DR3X]
• GSE319708 脑移植的单核细胞来源的血液和颅骨骨髓巨噬细胞表现出不同的特性 [scRNA_FIRE-ctrl-blood-skull]
• GSE319167 脑移植的血液和颅骨骨髓单核细胞衍生巨噬细胞表现出不同的特性 [Cut-n-Tag]
• GSE313388 脑移植单核细胞来源的血液和颅骨骨髓巨噬细胞表现出不同的特性 [bulkRNA_braintissue]
• GSE313387 脑移植的单核细胞来源巨噬细胞（来自血液和颅骨骨髓）表现出不同的特性 [bulkRNA_isolatedBrainParenchymalMacrophages]
• GSE312597 尿液细胞转录组基准测试，用于非侵入性区分BK多瘤病毒相关肾病和T细胞介导的排斥反应
• GSE307732 非编码 SNP rs17728461 通过染色体间调控 RAB27A 表达来调节肺癌进展
• GSE306122 BacNav基因疗法通过调节钠/钙稳态改善慢性心力衰竭患者的心脏功能障碍
• GSE291655 褪黑素类似物阿戈美拉汀通过激活SIRT1/FoxO1/ULK1信号通路介导的线粒体自噬，缓解缺氧诱导的肺动脉高压
• GSE316460 RNA-seq 分析野生型成纤维细胞和携带致病性 EPG5 突变的患者成纤维细胞