科研日报 2026-03-02

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📅 Daily Report - 2026-03-02

今日筛选出 745 条内容，来自 2 个来源

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🤖 今日AI智能总结

🧬 数据前沿

今日焦点： 研究揭示IL-17驱动的卵巢癌微环境重塑及IL-1beta/IL-23信号通路在抵抗非结核分枝杆菌中的关键作用。

主要方向：

  • 肿瘤免疫微环境调控：聚焦胆管癌、卵巢癌、淋巴结和肾移植排斥反应中的免疫细胞互作和信号通路。
  • 疾病机制探索：深入解析巨噬细胞在非结核分枝杆菌感染、主动脉瓣狭窄进展及脑膜病变中的作用。
  • 致病性血管通路研究：关注高内皮小静脉（HEVs）在肿瘤免疫逃逸中的作用。

技术亮点：

  • 空间转录组学（Spatial Transcriptomics）和单细胞RNA测序（scRNA-seq）技术广泛应用于解析复杂生物体系的细胞异质性和空间信息。
  • PRO-cap技术用于高精度绘制基因转录起始位点。

🧪 博客更新

今日焦点： 科学家发现新型细菌“杀毒开关”有望对抗超级细菌；咖啡与CRISPR基因编辑结合，或可用于癌症治疗。

主要方向：

  • 探索药物（Ozempic/Wegovy）新成分（SNAC）对肠道健康的影响。
  • 开发针对耐药细菌的新型治疗策略。
  • 利用基因编辑技术实现癌症细胞的靶向干预。

技术亮点：

  • 发现病毒利用特定机制关闭细菌关键蛋白，为开发新型抗生素提供思路。
  • 整合咖啡因与CRISPR技术，构建细胞程序化调控系统。

📚 分类浏览

🧬 数据前沿 (742条)

详细内容（前10条）

1. ⭐ GSE316402 帕博利珠单抗联合粒细胞巨噬细胞集落刺激因子治疗晚期胆道癌的 II 期试验 [空间转录组学]

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：cancer、macrophage、spatial、spatial transcriptomics、transcriptomics
  • 📝 描述：Contributors : Robin K Kelley ; Paige Bracci ; John D Gordan ; Spencer C Behr ; Zoe Quandt ; Chloe E Atreya ; Wesley Kidder ; Andrew H Ko ; Huat Lim ; Katherine Van Loon ; Nia Adeniji ; Alan P Venook ; Lawrence Fong ; Bridget P KeenanSeries Type : OtherOrganism : Homo sapiensImmune checkpoint inhibitors have limited activity as monotherapy in biliary cancers. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic immune cell growth factor which resulted in prolonged survival when combined with ipilimumab in melanoma. We conducted a single-center, phase II trial to evaluate the efficacy and safety of combining GM-CSF with pembrolizumab in patients with advanced biliary cancers after prior standard chemotherapy but no prior immune checkpoint inhibitor. Pembrolizumab 200 mg was administered intravenously in 21-day cycles, along with two cycles of GM-CSF 250 µg subcutaneously days 1 through 14. The primary endpoint was objective response rate. Among 42 patients enrolled, the median age was 61 years, 67% had intrahepatic cholangiocarcinoma, 90% had stage IV disease, and 24% had underlying viral hepatitis. The confirmed objective response rate was 12% (95% confidence interval: 4, 26), including two patients with complete response, and 26% of patients had progression-free survival ongoing at 6 months. Treatment was well-tolerated with treatment-related grade 3-4 events in 7% and treatment-related serious adverse events in 10%. Tumor PD-L1 expression was present in 46% and was associated with a higher rate of 6-month progression-free survival. Paired tumor biopsies showed upregulation of CD8+ T cell populations and antigen processing pathways after the addition of GM-CSF. In conclusion, the addition of GM-CSF to pembrolizumab was well-tolerated but did not meet the pre-specified response rate for efficacy. A subset of patients experienced deep responses and prolonged stable disease. GM-CSF elicited changes in the tumor immune microenvironment that could guide future combination approaches.
  • 🔗 查看原文

2. ⭐ GSE319959 IL-17 驱动的肿瘤细胞内在炎症编程在卵巢透明细胞癌中创造了有利于免疫疗法的微环境 [scRNA-Seq]

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：tumor、carcinoma、regex:immuno(logy|therapy|suppression)、scRNA
  • 📝 描述：Contributors : Kosuke Murakami ; Shiki Takamura ; Chiho Miyagawa ; Kazuhiro Kakimi ; Noriomi MatsumuraSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusOvarian clear cell carcinoma (OCCC) is a chemoresistant subtype of ovarian cancer with limited effective therapeutic options. Although immune checkpoint inhibitors (ICIs) have shown little benefit in unselected ovarian cancer populations, clinical activity observed in a subset of OCCC patients suggests the presence of a histotype-specific immune vulnerability that remains poorly defined. To elucidate this biology, we integrated transcriptomic analyses from multiple independent human OCCC cohorts with immunohistochemical profiling and mechanistic studies using an immunocompetent syngeneic OCCC mouse model. Tumor cell–intrinsic and microenvironmental responses to IL-17 were investigated through in vitro stimulation assays, in vivo IL-17 administration, single-cell RNA sequencing of tumor-infiltrating T cells, and immune checkpoint blockade in a Th17-biased partial chimera model. OCCC was characterized as an IL-6–high tumor entity with a globally immune-sparse but CD4⁺ T-cell–skewed tumor immune microenvironment. Transcriptomic analyses identified a Th17-associated program marked by elevated RORC expression in a subset of tumors. Across large clinical cohorts, an IL17A^high subset demonstrated a T cell–inflamed gene expression profile independent of microsatellite instability and tumor mutational burden; however, this phenotype was not associated with survival in untreated disease. Mechanistically, IL-17 directly activated NF-κB–dependent inflammatory programs in OCCC tumor cells, inducing cytokines involved in T-cell recruitment and activation. In vivo, IL-17 remodeled the immune microenvironment, increasing infiltration and activation of both CD4⁺ and CD8⁺ T cells. Single-cell profiling further revealed expansion of Th17/Tfh-like CD4⁺ T cells and cytotoxic, non-terminally exhausted CD8⁺ T cells. Consistent with these findings, anti–PD-L1 therapy significantly improved survival exclusively in Th17-biased partial chimera mice. Collectively, these results demonstrate that a Th17-biased, IL-17–responsive immune contexture in OCCC engages tumor cell–intrinsic NF-κB inflammatory signaling to prime antitumor T-cell states and enable checkpoint blockade efficacy. This immune biology is predictive rather than prognostic, supporting a biomarker-driven immunotherapy strategy for OCC…
  • 🔗 查看原文

3. ⭐ GSE290965 巨噬细胞介导的 IL1beta/IL23 信号传导调节针对非结核分枝杆菌的 γ δ (γδ) T 细胞免疫 [scRNA-seq]。

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：immunity、T cell、macrophage、scRNA
  • 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMechanistic Insights. Our study reveals the crucial role of gammadelta T cells in non-tuberculous mycobacteria (NTM) infection. We observed a significant increase and activation of gammadelta T cells in mice infected with MAB or with MAB infection combined with pulmonary fibrosis. Depletion of gammadelta T cells worsened the infection, while transfer of gammadelta T cells reversed this effect. Mechanistically, we found that MAB infection stimulates macrophages to produce IL-1beta and IL-23, which promotes the expansion of gammadelta T17 cells. MAB can also directly activate gammadelta T cells, leading to the clearance of MAB through an IL-17A-dependent pathway. Our findings suggest that gammadelta T cells represent a potential therapeutic target for NTM infections.
  • 🔗 查看原文

4. ⭐ GSE290963 巨噬细胞介导的 IL1beta/IL23 信号传导调节针对非结核分枝杆菌的 γ δ (γδ) T 细胞免疫 [RNA-seq]。

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：immunity、T cell、macrophage、RNA-seq
  • 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMechanistic Insights. Our study reveals the crucial role of gammadelta T cells in non-tuberculous mycobacteria (NTM) infection. We observed a significant increase and activation of gammadelta T cells in mice infected with MAB or with MAB infection combined with pulmonary fibrosis. Depletion of gammadelta T cells worsened the infection, while transfer of gammadelta T cells reversed this effect. Mechanistically, we found that MAB infection stimulates macrophages to produce IL-1beta and IL-23, which promotes the expansion of gammadelta T17 cells. MAB can also directly activate gammadelta T cells, leading to the clearance of MAB through an IL-17A-dependent pathway. Our findings suggest that gammadelta T cells represent a potential therapeutic target for NTM infections.
  • 🔗 查看原文

5. ⭐ GSE318650 scRNA测序数据来自CTL组、巨噬细胞清除后5天和8周的软脑膜、皮质和硬脑膜细胞。

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：macrophage、sequencing、scRNA
  • 📝 描述：Contributors : Maximilian Fliegauf ; Lukas Amann ; Jovana Cupovic ; Roman Sankowski ; Marco PrinzSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPerivascular and leptomeningeal macrophages are non-parenchymal macrophages summarized as central nervous system (CNS)-associated macrophages (CAMs) that mediate immune responses at brain boundaries. Both, CAMs and juxtaneuronal microglia are derived from prenatal yolk sac (YS) precursors, long-living and maintain their populations by homeostatic self-renewal without input from the periphery. Whereas microglia have been shown to be repopulated by CNS endogenous remnants of the same lineage following depletion, the renewal biology of CAMs is still poorly understood. Here, by combining multilineage myeloid fate mapping, bulk and single-cell profiling and high resolution confocal imaging, we show that the repopulation is strikingly different between CAMs and microglia. In contrast to microglia, CAMs do not renew exclusively cell-autonomously, but transiently utilize CCR2+Ly-6C+ monocytes after niche induction in an integrin-dependent manner. Remarkably and unlike repopulated microglia, replenished monocyte-derived CAMs remain transcriptionally and functionally distinct from their YS-derived counterparts. Finally, we established a protocol that allows to selectively exchange CAMs modulating disease response without functionally affecting parenchymal microglia. These new insights into the biology of the CNS immune system offer completely new therapeutic avenues for diverse neuroinflammatory and neurodegenerative diseases.
  • 🔗 查看原文

6. ⭐ GSE308452 血浆蛋白质组学可改善癌症患者的血栓形成预测，并揭示一条可靶向的IL-17驱动的内皮细胞激活通路

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：cancer、proteomics、pathway
  • 📝 描述：Contributors : Brake Marisa ; Schulman Sol ; Zwicker JeffSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThrombosis remains a major cause of morbidity and mortality in cancer patients. Existing risk models fail to reliably predict venous thromboembolism (VTE), underscoring the need for more accurate predictive models. In this study, we conducted a high-throughput proteomic analysis of 1,105 plasma proteins from newly diagnosed lung and gastric cancer patients prospectively monitored for VTE development. Utilizing a Bayesian probabilistic machine learning approach, we developed a predictive model incorporating 11 protein biomarkers and five clinical parameters (age, sex, history of VTE, body mass index, and hemoglobin), which significantly outperformed the Khorana prediction model. Orthogonal validation in an external placebo cohort from a phase III trial confirmed the model’s predictive power. Further investigation into the mechanistic role of CD200R1, a checkpoint receptor limiting leukocyte inflammatory response that contributed strongly to the model, showed that reduced levels in plasma correlated with higher D-dimer and thrombosis risk. In CD200R1-deficient mice, elevated thrombin-antithrombin complexes confirmed a prothrombotic state characterized by increased IL-17A and endothelial inflammation. Administration of anti-IL-17A antibodies to CD200R1 deficient mice normalized thrombin generation in vivo, and a meta-analysis of human clinical IL-17A inhibitory antibody studies confirmed antithrombotic activity. These findings improve the prediction of thrombosis in cancer and highlight the utility of plasma proteomics to identify unanticipated mechanistic insights and therapeutic targets in thrombo-inflammatory disease.
  • 🔗 查看原文

7. ⭐ GSE306742 肿瘤相关高内皮静脉通过 ALOX12 驱动淋巴结门静脉特异性免疫逃逸 [转移性淋巴结]

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：immune、lymph、regex:lymph(o|atic)?
  • 📝 描述：Contributors : Qidong Xia ; Shicheng SuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHigh-endothelial-venules (HEVs) offer another entry point for tumor metastasis in mice. To investigate whether HEVs and non-specialized blood vessels exert different effects on disseminating tumor cells, we enriched EO771 derivatives HEVM3 (preferentially metastasize through HEVs) cells and BVM3 (preferentially metastasize through blood vessels) cells. To further explore potential factor(s) that influence tumor cells, we isolated blood vessel endothelial cells (BECs) in metastatic lymph nodes from six C57BL/6J mice and analyzed them with single-cell RNA sequencing.
  • 🔗 查看原文

8. ⭐ GSE306111 人类FALD和正常肝脏的空间转录组学

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
  • 📝 描述：Contributors : Seiya Kato ; Hayato Hikita ; Tetsuo TakeharaSeries Type : OtherOrganism : Homo sapiensSpatial transcriptomic analysis was performed on Fontan-associated liver disease (FALD) and normal livers using CosMx Spatial Molecular Imager.
  • 🔗 查看原文

9. ⭐ GSE303904 单细胞转录组学揭示肾移植排斥反应的早期免疫图谱，并指出 Ccl3-Ccr5 为治疗靶点

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：immune、single-cell、transcriptomics
  • 📝 描述：Contributor : Lijun YangSeries Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusBackground Acute rejection is a primary determinant of long-term allograft survival, and the early-stage immune cell dynamics with local intercellular communication during this process require further elucidation.Methods We performed single-cell RNA sequencing (scRNA-seq) on CD45+ immune cells isolated from rat renal allografts during the early phase of acute rejection (days 0, 1, 3, and 7). Using unsupervised clustering, functional enrichment analysis, cellular trajectory inference, and intercellular communication network mapping, we delineated the immune cell dynamics and local communication networks at single-cell resolution. Our findings were subsequently validated through multiplex immunofluorescence and therapeutic intervention experiments.Results Macrophages constituted the dominant immune population during acute rejection. Sub-clustering analysis revealed a rapid expansion of the Isg15+Mac subset by post-transplant day 1, which persisted at elevated levels thereafter. Functional enrichment and trajectory inference demonstrated the pro-inflammatory properties of Isg15+Mac, implicating this subset in acute rejection. Cell-cell communication analysis identified Ccl3-Ccr5 ligand-receptor interactions between Isg15+Mac and T cells. Multiplex immunofluorescence confirmed abundance of Isg15+Mac within the allografts. Moreover, We alleviated the acute rejection after kidney transplantation using the FDA-approved Ccr5 blocker Maraviroc.Conclusions Our study establishes an in-depth, early-stage immune landscape of renal transplantation, uncovering the Isg15+Mac subset as a critical driver of acute rejection via the Ccl3-Ccr5 axis. And also indicating that Maraviroc may potentially be a therapeutic candidate for transplant rejection.
  • 🔗 查看原文

10. ⭐ GSE291890 单细胞 RNA 测序揭示巨噬细胞参与主动脉瓣狭窄进展

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：macrophage、sequencing、single-cell
  • 📝 描述：Contributors : Tomoyo Hamana ; Takuo EmotoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIntroduction: Calcific aortic stenosis (AS) is an age-related progressive disease with increasing global prevalence. Although chronic inflammation is a key factor in its pathogenesis, the detailed immune cell profiles and their interactions with non-immune cells remain poorly understood. This study aimed to characterize the immune cell landscape in calcific AS at a single-cell level and evaluate the potential of anti-inflammatory therapy. Methods: Human aortic valve samples were obtained from four patients with non-calcified aortic regurgitation (AR) and 11 patients with AS undergoing surgical aortic valve replacement. Single-cell 3’ gene expression sequencing (scRNA-seq (3’)) was performed on CD45+ immune cells, while single-cell RNA sequencing Flex (scRNA-seq (Flex)) and bulk RNA sequencing were conducted on unsorted cells. Additionally, an anti-TNFα antibody experiment was performed in a murine AS model induced by aortic valve wire injury. Results: In myeloid cells, LYVE1 resident macrophages were significantly decreased, whereas classical monocytes expressing pro-inflammatory genes and C1Q macrophages were increased in AS valves compared to AR valves. These macrophages exhibited strong interactions with valve interstitial cells, particularly with chondrogenic subtypes expressing COMP and SPARC. Anti-TNFα treatment in the murine model significantly reduced aortic valve calcification without hematopoietic complications. Conclusions: This study is the first to integrate scRNA-seq (3’) and scRNA-seq (Flex) analyses of human aortic valves, revealing single cell-level characteristics of immune cells and their interactions with non-immune cells in calcific AS. These findings suggest that anti-inflammatory therapy could be a therapeutic target for calcific AS.
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💡 该来源还有 732 条内容，详见 文末

🧪 博客更新 (3条)

详细内容（全部3条）

1. Ozempic 和 Wegovy 片剂中的隐藏成分引发了新的肠道健康问题

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：gut、regex:gut(-?microbiome)?
  • 📝 描述：Scientists are taking a closer look at the pill forms of Wegovy and Ozempic. In an animal study, the ingredient SNAC, which helps semaglutide survive the stomach and enter the bloodstream, was associated with changes in gut bacteria, inflammation markers, and a brain linked protein. The research does not show harm in people, but it raises new questions about the long term effects of daily exposure.
  • 🔗 查看原文

2. 科学家发现细菌“自杀开关”，或将改变对抗超级细菌的斗争。

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：regex:bacter(ia|ial|ium)
  • 📝 描述：Drug-resistant bacteria are becoming harder to treat, pushing scientists to look for new antibiotic targets. Researchers have now discovered that several unrelated viruses disable a key bacterial protein called MurJ, which is essential for building the bacterial cell wall. High-resolution imaging shows these viral proteins lock MurJ into a single position, stopping cell wall construction and leading to bacterial death.
  • 🔗 查看原文

3. 你早晨喝的咖啡或许有一天能帮助对抗癌症。

  • ✍️ 作者：未知作者
  • 🏷️ 关键词：cancer
  • 📝 描述：Scientists at Texas A&M are turning an everyday pick-me-up into a high-tech medical switch. By combining caffeine with CRISPR gene editing, researchers have created a system that allows cells to be programmed in advance — and then activated simply by consuming a small dose of caffeine from coffee, chocolate, or soda. The approach, known as chemogenetics, lets scientists precisely turn gene-editing activity on and off inside targeted cells, including powerful immune T cells that can fight cancer.
  • 🔗 查看原文

📊 关键词统计

关键词出现次数
T cell375
scRNA78
macrophage72
RNA-seq52
monocyte33
carcinoma26
B cell22
sequencing18
cancer15
ATAC-seq11
immune10
lymph9
regex:lymph(oatic)?
leukemia8
single-cell7
cardiac6
immunity6
transcriptome5
ChIP-seq5
metabolic5

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🧬 数据前沿 其他内容 (732条)

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