详细内容（前10条）

1. ⭐ GSE285606 miR-29a/b1簇的缺失重编程肿瘤微环境并导致肺癌免疫抑制

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、tumor microenvironment、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Jessica Konen ; Yanhua Tian ; Yuanxin XiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusImmune checkpoint inhibitors (ICI), like those that block PD-1/PD-L1, have revolutionized oncological therapy for patients with non-small cell lung cancer (NSCLC). However, most patients demonstrate no clinical benefit or acquire resistance, even in PD-L1+ populations, highlighting the critical need to dissect tumor survival dependencies to overcome resistance. Using our unique Kras/p53-driven lung cancer models which demonstrate acquired or intrinsic resistance to ICI, we performed single cell RNA-sequencing and focused on predicted upstream regulators of differentially expressed genes (DEGs) in the malignant cell cluster of resistant tumors. We found that the micro-RNA, miR-29, was downregulated in tumors with anti-PD-1 resistance, leading to significant upregulation of a multitude of miR-29 targets. Interestingly, we found that the immunosuppressive molecule Enpp2/ATX was one of these genes modulated due to miR-29 loss, and re-expression of miR-29 in anti-PD-1 resistant models diminished ATX tumor expression, diminished the fibrotic microenvironment, and increased CD8 infiltration. Additionally, analysis of lung adenocarcinoma patients revealed miR-29-high patients had increased CD8A expression and enrichment in immunoregulatory pathways. Together, these data provide evidence that the miR-29 family broadly regulates the tumor microenvironment, including anti-tumor immune-related pathways in lung cancer, through control of ATX among many other target genes, with implications in ICI response.
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2. ⭐ GSE275785 双重靶向 STING 和 PI3Kγ 可消除调节性 B 细胞以克服胰腺癌免疫疗法的 STING 耐药性 [scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、regex:immuno(logy|therapy|suppression)、resistance、scRNA
• 📝 描述：Contributors : Chengyi Li ; Duxin SunSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe immune suppression in tumors and lymph nodes of pancreatic ductal adenocarcinoma (PDAC), regulated by suppressive myeloid cells and regulatory B (Breg) cells, hinders the effectiveness of immunotherapy. Although STING agonists activate myeloid cells to overcome immune suppression, it expands Breg cells, conferring STING resistance in PDAC. We discovered that blocking PI3Kγ during STING activation abolished IRF3 phosphorylation to eliminate Breg cells, while PI3Kγ inhibition sustained STING-induced IRF3 phosphorylation to preserve STING function in myeloid cells. Therefore, we developed a dual functional compound SH-273 and its albumin nanoformulation Nano-273, which stimulates STING to activate myeloid cells and inhibits PI3Kγ to eliminates Breg cells overcoming STING resistance. Nano-273 achieved systemic antitumor immunity through intravenous administration, which decreases Breg cells and remodels microenvironment in tumors and lymph nodes. Nano-273, combined with anti-PD-1, extended median survival to 200 days in transgenic KPC PDAC mice (KrasG12D-P53R172H-Cre), offering potential for PDAC treatment.
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3. ⭐ GSE275780 双重靶向 STING 和 PI3Kγ 可消除调节性 B 细胞以克服胰腺癌免疫疗法的 STING 耐药性 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、regex:immuno(logy|therapy|suppression)、resistance、RNA-seq
• 📝 描述：Contributors : Chengyi Li ; Duxin SunSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe immune suppression in tumors and lymph nodes of pancreatic ductal adenocarcinoma (PDAC), regulated by suppressive myeloid cells and regulatory B (Breg) cells, hinders the effectiveness of immunotherapy. Although STING agonists activate myeloid cells to overcome immune suppression, it expands Breg cells, conferring STING resistance in PDAC. We discovered that blocking PI3Kγ during STING activation abolished IRF3 phosphorylation to eliminate Breg cells, while PI3Kγ inhibition sustained STING-induced IRF3 phosphorylation to preserve STING function in myeloid cells. Therefore, we developed a dual functional compound SH-273 and its albumin nanoformulation Nano-273, which stimulates STING to activate myeloid cells and inhibits PI3Kγ to eliminates Breg cells overcoming STING resistance. Nano-273 achieved systemic antitumor immunity through intravenous administration, which decreases Breg cells and remodels microenvironment in tumors and lymph nodes. Nano-273, combined with anti-PD-1, extended median survival to 200 days in transgenic KPC PDAC mice (KrasG12D-P53R172H-Cre), offering potential for PDAC treatment.
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4. ⭐ GSE319709 单细胞转录组学揭示肝细胞癌中病因特异性T细胞异质性并提示其调控机制

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、single-cell、transcriptomics
• 📝 描述：Contributor : Fengna YanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHepatocellular carcinoma (HCC) exhibits remarkable etiological heterogeneity, with hepatitis B virus (HBV) infection and metabolic dysfunction-associated steatohepatitis (MASH) emerging as two leading causes. The tumor microenvironment (TME), particularly T cell subsets, plays a pivotal role in tumor progression and immunotherapy response. However, the etiology-specific T cell landscapes in HBV-HCC and MASH-HCC remain poorly characterized.
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5. ⭐ GSE310857 用 SHMT2 敲低慢病毒（人）转染的胰腺癌细胞的转录组测序。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、sequencing、transcriptome
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe role of SHMT2 in driving pancreatic cancer progression remains incompletely understood. Our previous findings demonstrated that SHMT2 enhances glycolytic metabolism and promotes proliferative and invasive capacities in pancreatic cancer cells. To delineate the precise molecular mechanism underlying these observations, we performed transcriptomic sequencing.
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6. ⭐ GSE290093 CD36/FABP5/PPARδ信号轴的空间转录组学定义了新生儿期自发增殖的心肌细胞

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Abou Bakr M Salama ; Tamer M MohamedSeries Type : OtherOrganism : Mus musculusSpontaneously cycling cardiomyocytes (CMs) represent a rare and unique population in the neonatal heart with the potential to drive cardiac regeneration in adulthood. While previous studies have observed their existence, the mechanisms regulating their cell cycle activity remain poorly understood. Here, we aim to identify the signaling axis that defines the spontaneously proliferating CMs in the neonatal stage.
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7. ⭐ GSE225630 转移前肿瘤引流淋巴结的转录组，伴或不伴 TDLN 支配伤害性神经的去神经支配

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、lymph、regex:lymph(o|atic)?
• 📝 描述：Contributors : Yu Zhang ; Yibo Guo ; Tong JiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe compared the mice tumor-draining lymph nodes(TDLNs) with or without orthotopic tongue xenografts, and with or without denervation of TDLN-innervating nociceptive nerves
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8. GSE293149 衰老控制中精子发生特异性 Argonaute 蛋白和胰岛素/IGF-1 信号传导的相互调控 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、RNA-seq
• 📝 描述：Contributors : Thomas Liontis ; Valentina T Pannarale ; Andres R Mansisidor ; Sasiru K Pathiranage ; Jeeya Y Patel ; Alla GrishokSeries Type : Expression profiling by high throughput sequencingOrganism : Caenorhabditis elegansThe potential role of small interfering RNAs (siRNAs) produced from double-stranded RNA in aging has not been fully addressed.The networks of genes regulated by siRNAs and their partner Argonaute proteins are best understood in C. elegans, a pioneeringmodel of aging and small RNA studies. Here, we describe synergistic lifespan extension of insulin/IGF-1 signaling (IIS) mutant age-1(hx546) by rde-4 or alg-3; alg-4 deficiencies. By analyzing gene expression and siRNA populations in these IIS and RNAi mutants,we show here that redundant spermatogenesis-specific Argonautes ALG-3 and ALG-4 are capable of regulating IIS, potentially through direct control of the Major Sperm Protein (MSP) genes in the germline. MSPs and MSP domains of some mammalian proteins are secreted and directly inhibit the Eph receptor (EphR). In turn, EphR interacts with and destabilizes PTEN, a major negative regulator of IIS. We show that enhanced MSP expression correlates with EphR mislocalization and elevated PTEN levels in oocytes of alg-3/4(-) worms. At the same time, ALG-3/4 expression is regulated by IIS. Thus, we propose mutual regulation of IIS and ALG-3/4 through secreted ligands.
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9. GSE316734 子宫内膜癌和卵巢癌细胞系对DHX9抑制剂的敏感性和耐药机制的表征

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、resistance
• 📝 描述：Contributor : Huang Tzu-TingSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the DHX9i response across endometrial and ovarian cancer cell lines with diverse backgrounds, total RNA was isolated from three biological replicates of cells treated with or without ATX968 (a DHX9 inhibitor). The RNA samples were then subjected to paired-end RNA sequencing on the NovaSeq X Plus (Illumina) platform.
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10. GSE304899 B 细胞内在 I 型干扰素信号传导导致疫苗缺陷 [VDJ]

• ✍️ 作者：未知作者
• 🏷️ 关键词：vaccine、B cell
• 📝 描述：Contributors : Xavier Laulhé ; Yasmine Adda-Bouchard ; Guillaume Lopez ; Gabriel Chamberlain ; Anolie Dubreuil ; Tania Charpentier ; Alain LamarreSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAffinity maturation and vaccine efficacy are compromised during chronic viral infections, yet the underlying mechanisms remain unclear. Using the LCMV Cl13 model, we show that IFN-I signaling in B cells plays a central role. IFN-I promotes early B cell activation but reduces clonal diversity and delays IgG1⁺ B cell entry into germinal centers, impairing high-affinity clone selection. Deletion of IFNAR1 in B cells partially restores NP-specific IGHV1-72 usage and GC access but fails to fully rescue affinity maturation, suggesting a contribution of extrinsic factors. Somatic hypermutation remains elevated in both genotypes, though slightly reduced in IFNAR1⁻/⁻ B cells. BASELINe analysis indicates weaker selection pressure in CDRs, potentially reflecting impaired affinity-based selection. This defect correlates with a reduced TFR/TFH ratio. Our results show that intrinsic and extrinsic IFN-I-dependent mechanisms synergize to disrupt B cell fate. These findings establish IFN-I as a key regulator of humoral immunity and highlight mechanisms underlying poor vaccine response.
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1. ⭐ scHiCAR——转录组、表观基因组和3D基因组的三模态单细胞分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、genome、transcriptome、epigenome
• 📝 描述：scHiCAR integrates RNA sequencing, chromatin accessibility, and 3D genome mapping in single cells, enabling high-resolution analysis of enhancer promoter interactions across complex tissues…
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2. 科学家发现人体隐藏的炎症“关闭开关”。

• ✍️ 作者：未知作者
• 🏷️ 关键词：inflammation
• 📝 描述：A new human study has uncovered how the body naturally turns off inflammation. Researchers found that fat-derived molecules called epoxy-oxylipins rein in immune cells that can otherwise drive chronic disease. Using a drug to boost these molecules reduced pain faster and lowered harmful inflammatory cells. The discovery could pave the way for safer treatments for arthritis, heart disease, and other inflammation-related conditions.
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3. 原子级门控技术有望彻底改变DNA测序和神经形态计算。

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Scientists have taken a major step toward mimicking nature’s tiniest gateways by creating ultra-small pores that rival the dimensions of biological ion channels—just a few atoms wide. The breakthrough opens new possibilities for single-molecule sensing, neuromorphic computing, and studying how matter behaves in spaces barely larger than atoms.
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4. 科学家在流行的假发片中发现致癌化学物质

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：A sweeping new study has uncovered a troubling mix of hazardous chemicals in popular hair extensions, including products made from human hair. Researchers detected dozens of substances linked to cancer, hormone disruption, reproductive harm, and immune system effects in nearly every sample tested. Some products contained flame retardants, organotins, and chemicals associated with increased breast cancer risk, and several exceeded European safety thresholds.
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• GSE304898 B 细胞内在 I 型干扰素信号传导导致疫苗缺陷 [GEX]
• GSE319605 人类心脏 Treg 细胞的单细胞 RNA 测序 (SORT-seq) (QMU-SF-s001–QMU-SF-s012)
• GSE319453 糖尿病和脓毒症小鼠肠道转录组分析 [RNA-seq]
• GSE319365 肺泡巨噬细胞的训练免疫通过增强 KLF4-MERTK 介导的胞吞作用促进损伤消退 [ATAC-seq 72h post-LPS]
• GSE300520 面部浸润性脂肪瘤或对照组分离的脂肪组织转录组测序
• GSE294332：对阿尔茨海默病小鼠模型进行多组学单核分析表明，ACSS2赋予神经元抵抗tau蛋白病变的能力。
• GSE290127 TAK1信号通路对心脏成纤维细胞转录组的影响
• GSE304872 吉兰-巴雷综合征早期外周神经靶向和疼痛促进转录组特征 [scRNA-seq]
• GSE304871 吉兰-巴雷综合征早期外周神经靶向和疼痛促进转录组特征[批量RNA测序]
• GSE303753 通过增强代谢通量促进生育酚生物合成和质体球增殖来强化叶片化学。
• GSE293150 衰老控制中精子发生特异性 Argonaute 蛋白和胰岛素/IGF-1 信号传导的相互调控 [smallRNA-Seq]
• GSE270345 犬乳管乳头状乳腺肿瘤转录组
• GSE316426 MondoA介导MYC网络与胰腺导管腺癌整合应激反应之间的转录协调[RNA-Seq]
• GSE307746 急性季节性流感感染期间下呼吸道中人 CD8+ T 细胞的反应
• GSE289318 WNT介导的肠上皮转化过程中基质Pdgfra表达细胞的动态重编程 - 图3J
• GSE274811 RNA测序：过表达锚定MafG-MITF (T-MGM) 的WM164细胞
• GSE295103 小鼠肾脏出生后发育过程中的单核 RNA 测序。
• GSE319738 恶唑烷酮介导的翻译抑制的上下文特异性受抗生素结构调控 [RNA-Seq]
• GSE319710 单核细胞通过血小板相互作用和氧化应激信号传导而成为 HIV 相关动脉粥样硬化的基础 [scRNA-seq]
• GSE319557 灵长类岛叶皮层细胞图谱和新型信号处理策略 [scRNA-seq]
• GSE319556 内皮细胞 TLR4 信号传导驱动新生儿脑膜炎小鼠模型中的组织炎症、Claudin-5 内化和血管屏障破坏
• GSE302847 铁木皂苷AIII通过抑制CAR-Tr​​eg II增强CAR-T细胞效力并预防复发
• GSE290002 CDK8/19抑制剂在结直肠癌中的选择性和活性的确认[体外]
• GSE290001 CDK8/19抑制剂在结直肠癌中的选择性和活性的确认[体内]
• GSE279009 小鼠胚胎心脏中PCBP1的增强型交联免疫沉淀测序（eCLIP-seq）
• GSE279008 胚胎心脏转录组，心肌细胞特异性缺乏 Pcbp1
• GSE279007 成年心脏中Aars2特异性心肌细胞缺陷的转录组
• GSE279006 心肌细胞特异性Aars2缺陷的胚胎心脏转录组
• GSE256144 RNA-seq 在 DDX24 敲低后 HUVECs 中的应用