详细内容（前10条）

1. ⭐ GSE301265 空间转录组学揭示多灶性小肠神经内分泌肿瘤的局部亚型特异性特征和信号传导

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics、regex:intestin(e|al)
• 📝 描述：Contributors : Akitada Yogo ; Naoki Akanuma ; Grace E Kim ; Eric K NakakuraSeries Type : OtherOrganism : Homo sapiensSmall intestinal neuroendocrine tumors (SI-NETs) frequently present as multifocal lesions, but the spatial and molecular mechanisms underlying their development and heterogeneity remain unclear. This study aimed to characterize the phenotypic subtypes of tumor cells across anatomical sites in multifocal SI-NETs and identify local microenvironmental factors influencing tumor development.
• 🔗 查看原文

2. ⭐ 利用CosMX空间转录组学分析小鼠皮下EO771肿瘤，研究NEDD8耗竭和抗PD-1疗法的协同作用（GSE318797）。

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Papakyriacou Irineos ; Lu Yonglin ; Bedos Marta Rubies ; Jarvius Malin ; Lapins Maris ; Puigvert Jordi Carreras ; Mao YuMaoSeries Type : OtherOrganism : Mus musculusWe have established the phenotype that depletion of NEDD8 in tumor cell has synergestic effect with anti-PD1 therapy in mouse EO771 subcutaneous tumor model. In this study we performed CosMX spatial transcriptomics of mouse tumor samples to study the tumor intrinsic and immune environment changes that could explain the outcome.
• 🔗 查看原文

3. ⭐ GSE264319 分析干细胞来源的心脏类器官对免疫检查点抑制剂的免疫非依赖性反应 [scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、cardiac、scRNA
• 📝 描述：Contributors : Dilip Thomas ; Yu LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe use of antibody based immune-checkpoint inhibitors (ICIs) have been highly successful in clinical treatment of several cancers. Despite the compelling efficacy of these drugs with their high binding affinity, there is incomplete understanding of non-target interactions in vivo. In this regard, ICIs are also known to cause immune-related adverse events (iRAEs) arising from infiltration of immune cells in tissues such as the heart. Due to the frequency of ICI treatments, extended half-life and the late onset of cardiac events, concerns have been raised regarding potential direct interactions of ICIs with major cellular cell types of the heart. The direct effects of these drugs in patients are limited due to rare incidence, and difficulty in obtaining patient heart biopsies in the pre- or post-symptomatic phase. Therefore, in order to study both functional and molecular changes in vitro, here we utilized a human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and multicellular cardiac organoids (COs).
• 🔗 查看原文

4. ⭐ GSE318809 通过单细胞转录组学解析脆弱拟杆菌中噬菌体-宿主相互作用的动态[批量RNA-Seq数据]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、single-cell、transcriptomics
• 📝 描述：Contributors : Norma Morella ; Neelendu Dey ; Anika Gupta ; Dmitry Sutormin ; Anna KuchinaSeries Type : Expression profiling by high throughput sequencingOrganism : Bacteroides fragilisSuccess of phage therapies is limited by bacterial defenses against phages. While a variety of anti-phage defense mechanisms has been characterized, how expression of these systems is distributed across individual cells and how their combined activities translate into protection from phages has not been studied. Using bacterial single-cell RNA sequencing, we profiled the transcriptomes of ~50,000 cells from cultures of a human pathobiont, Bacteroides fragilis, infected with a lytic bacteriophage. We quantified the asynchronous progression of phage infection in single bacterial cells and reconstructed the infection timeline, characterizing both host and phage transcriptomic changes as infection unfolded. We discovered subpopulations of bacteria that remained uninfected and heterogeneously expressed protective factors. Each cell’s vulnerability to phage infection was defined by combinatorial expression of multiple genetic loci, including phase-variable capsular polysaccharide (CPS) biosynthesis pathways, restriction-modification systems (RM), and a novel operon predicted to encode fimbrial genes. Acting in concert, these heterogeneously expressed anti-phage defense mechanisms create a phenotypic landscape where distinct protective combinations enable the survival and re-growth of bacteria expressing these phenotypes without acquiring additional mutations. The emerging model of complementary action of multiple protective mechanisms heterogeneously expressed across an isogenic bacterial population showcases the potent role of phase variation and stochasticity in bacterial anti-phage defenses.
• 🔗 查看原文

5. GSE309282 单细胞 RNA 测序 (scRNA-seq) 研究了小鼠第一磨牙及其邻近牙槽骨组织在正畸负荷作用下和未负荷作用下的 RNA 水平变化。

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、scRNA
• 📝 描述：Contributors : Miao Tan ; Minyu HeSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSingle-cell RNA sequencing (scRNA-seq) was performed on mouse first molar and adjacent alveolar bone tissues (within 1 mm, buccal and palatal sides). Two groups were analyzed: non-loaded control and orthodontic tooth movement (OTM) after 1 day. The study aims to reveal early cellular and transcriptional changes induced by orthodontic force.
• 🔗 查看原文

6. GSE300367 分析干细胞来源的心脏类器官对免疫检查点抑制剂的免疫非依赖性反应 [scRNAseq_Mouse]

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、cardiac
• 📝 描述：Contributors : Dilip Thomas ; Yu LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe use of antibody based immune-checkpoint inhibitors (ICIs) have been highly successful in clinical treatment of several cancers. Despite the compelling efficacy of these drugs with their high binding affinity, there is incomplete understanding of non-target interactions in vivo. In this regard, ICIs are also known to cause immune-related adverse events (iRAEs) arising from infiltration of immune cells in tissues such as the heart. Due to the frequency of ICI treatments, extended half-life and the late onset of cardiac events, concerns have been raised regarding potential direct interactions of ICIs with major cellular cell types of the heart. The direct effects of these drugs in patients are limited due to rare incidence, and difficulty in obtaining patient heart biopsies in the pre- or post-symptomatic phase. Therefore, in order to study both functional and molecular changes in vitro and in vivo, we utilized a human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs), cardiac organoids (COs), and immunocompromised NOD/SCID mouse models.
• 🔗 查看原文

7. GSE296370 儿童和青少年伯基特白血病和淋巴瘤中的 miRNA 特征

• ✍️ 作者：未知作者
• 🏷️ 关键词：leukemia、lymphoma
• 📝 描述：Contributors : Björn Schneider ; Teresa M Cardesa-SalzmannSeries Type : Expression profiling by arrayOrganism : Homo sapiensmiRNA expression analysis in Burkitt leukemia and lymphoma in children and young adults. Comparison of clinicopathological subgroups. Evaluation of differential expression.
• 🔗 查看原文

8. GSE263840 分析干细胞来源的心脏类器官对免疫检查点抑制剂的非免疫依赖性反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、cardiac
• 📝 描述：Contributors : Dilip Thomas ; Yu LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe use of antibody based immune-checkpoint inhibitors (ICIs) have been highly successful in clinical treatment of several cancers. Despite the compelling efficacy of these drugs with their high binding affinity, there is incomplete understanding of non-target interactions in vivo. In this regard, ICIs are also known to cause immune-related adverse events (iRAEs) arising from infiltration of immune cells in tissues such as the heart. Due to the frequency of ICI treatments, extended half-life and the late onset of cardiac events, concerns have been raised regarding potential direct interactions of ICIs with major cellular cell types of the heart. The direct effects of these drugs in patients are limited due to rare incidence, and difficulty in obtaining patient heart biopsies in the pre- or post-symptomatic phase. Therefore, in order to study both functional and molecular changes in vitro, here we utilized a human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and multicellular cardiac organoids (COs).
• 🔗 查看原文

9. GSE303002 SMARCA5–DMRT1 先锋复合物建立表观遗传启动以指导雄性生殖细胞发育 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、epigenetic
• 📝 描述：Contributors : Satoshi H Namekawa ; Yuka Kitamura ; Yasuhisa MunakataSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusEstablishing cell type–specific chromatin landscapes is essential for cellular identity, yet how these landscapes are generated and maintained remains poorly understood. Here, we demonstrate that the chromatin remodeler SMARCA5 facilitates epigenetic priming required for retinoic acid–induced differentiation in the male germline. Germ cell–specific deletion of Smarca5 results in a complete loss of differentiating spermatogonia, phenocopying vitamin A–deficient mice lacking retinoic acid signaling. During the perinatal transition from prospermatogonia to undifferentiated spermatogonia, SMARCA5 is recruited to DMRT1-binding sites located at distal putative enhancers and promoters of germline genes. The SMARCA5–DMRT1 pioneer complex establishes chromatin accessibility at these loci, generating poised enhancers and promoters that serve as retinoic acid receptor (RAR)–binding sites. Thus, SMARCA5 licenses transcriptional responses to retinoic acid that enable spermatogenic differentiation. Our findings uncover a critical epigenetic priming mechanism that links pioneer factor activity to external signal responsiveness in the germline.
• 🔗 查看原文

10. GSE303001 SMARCA5–DMRT1 先锋复合物建立表观遗传启动以指导雄性生殖细胞发育 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：ATAC-seq、epigenetic
• 📝 描述：Contributors : Satoshi H Namekawa ; Yuka Kitamura ; Yasuhisa MunakataSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusEstablishing cell type–specific chromatin landscapes is essential for cellular identity, yet how these landscapes are generated and maintained remains poorly understood. Here, we demonstrate that the chromatin remodeler SMARCA5 facilitates epigenetic priming required for retinoic acid–induced differentiation in the male germline. Germ cell–specific deletion of Smarca5 results in a complete loss of differentiating spermatogonia, phenocopying vitamin A–deficient mice lacking retinoic acid signaling. During the perinatal transition from prospermatogonia to undifferentiated spermatogonia, SMARCA5 is recruited to DMRT1-binding sites located at distal putative enhancers and promoters of germline genes. The SMARCA5–DMRT1 pioneer complex establishes chromatin accessibility at these loci, generating poised enhancers and promoters that serve as retinoic acid receptor (RAR)–binding sites. Thus, SMARCA5 licenses transcriptional responses to retinoic acid that enable spermatogenic differentiation. Our findings uncover a critical epigenetic priming mechanism that links pioneer factor activity to external signal responsiveness in the germline.
• 🔗 查看原文

💡 该来源还有 42 条内容，详见 文末

详细内容（全部1条）

1. 一种秘密的细胞联盟或许可以解释卵巢癌为何如此致命。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have discovered why ovarian cancer spreads so rapidly through the abdomen. Cancer cells enlist normally protective abdominal cells, forming mixed groups that work together to invade new tissue. These helper cells lead the way, allowing cancer to spread faster and resist chemotherapy. The findings uncover a critical weakness that future treatments may target.
• 🔗 查看原文

• GSE297490 APP基因中与阿尔茨海默病相关的突变以性别依赖的方式影响结直肠肿瘤的形成
• GSE282025：博来霉素诱导肺纤维化模型中Tubb3-/-小鼠肺组织的单细胞RNA测序分析
• GSE264596 XPO7-NPAT轴代表TP53突变急性髓系白血病的关键脆弱性（scRNA-Seq）
• GSE304483 肝脏运动因子通过靶向脑血管逆转衰老和阿尔茨海默病相关的记忆丧失
• GSE317004 心肌梗死和NK细胞耗竭小鼠心脏CD45+白细胞的CITE-seq
• GSE307094 阿尔茨海默病成人神经发生模型中单细胞转录模式受损
• GSE285110 非坏死性凋亡 MLKL 功能损伤线粒体并促进造血干细胞衰老 [RNA-seq]
• GSE285109 非坏死性凋亡 MLKL 功能损伤线粒体并促进造血干细胞衰老 [ATAC-seq]
• GSE279282 跨祖先人群肾癌易感性因果关系的遗传景观和功能探索 [ATAC-seq]
• GSE317434 用对照 siRNA 或 FH siRNA 转染的 BJ 成纤维细胞的 RNA 测序
• GSE317294 整合转录组分析揭示了猪滋养层细胞中日本脑炎病毒感染的 miRNA 中心调控 [RNA-Seq]
• GSE316548 [18F]FDG PET/CT 多组学鉴定出 Hedgehog 驱动的 HPV 阴性头颈部鳞状细胞癌
• GSE300899 广泛的增强子串扰控制脂肪生成谱系定向过程中 PPARG2 表达的诱导 [Chip-Seq]
• GSE288814 表观转录组分析揭示了两种西瓜感染的 RNA 病毒中不存在 m6A 修饰 [GLORI-Seq]
• GSE288813 表观转录组分析揭示了两种西瓜感染的 RNA 病毒中不存在 m6A 修饰 [DRS]
• GSE288812 表观转录组分析揭示了两种西瓜感染的 RNA 病毒中不存在 m6A 修饰 [m6ARIPseq]
• GSE281778 MYC 与新生 RNA 的结合通过 R 环衍生的 RNA-DNA 杂交体抑制先天免疫信号传导 [seCLIP]
• GSE281614 RNA介导的MYC多聚化抑制先天免疫信号传导[mRNA]
• GSE281612 MYC 与新生 RNA 的结合通过 R 环衍生的 RNA-DNA 杂交体抑制先天免疫信号传导 [DRIPc]
• GSE281611 MYC 与新生 RNA 的结合通过 R 环衍生的 RNA-DNA 杂交体抑制先天免疫信号传导 [ChIP_Rx]
• GSE249809 RNA 5-甲基胞嘧啶写入酶NSUN5通过ZBED3依赖性机制促进肝细胞癌细胞增殖
• GSE318963 舒尼替尼通过抑制高级别浆液性卵巢癌细胞中的DNA修复通路与顺铂产生协同作用
• GSE318878 T细胞前淋巴细胞白血病中的microRNA网络反映T细胞活化并塑造DNA损伤反应和生存通路
• GSE308162 评估 miRomics 和整合蛋白质组学在鉴定血浆 miRNA 作为 IFNβ-1a 生物制剂潜在药效学生物标志物中的应用
• GSE303063 SMARCA5–DMRT1 先锋复合物建立表观遗传启动以指导雄性生殖细胞发育
• GSE303000 SMARCA5–DMRT1 先锋复合物建立表观遗传启动以指导雄性生殖细胞发育 [CUT&Tag]
• GSE300910 呼吸道合胞病毒感染通过诱导训练免疫和SARS-CoV-2反应性黏膜T细胞，诱导针对SARS-CoV-2的异源性保护
• GSE299672 PHIP抑制NuRD促进SWI/SNF突变癌症的生长[ChIP-seq]
• GSE299562 PHIP抑制NuRD促进SWI/SNF突变癌症的生长[RNA-seq]
• GSE296927 Notch配体Jagged1调控三阴性乳腺癌细胞外基质的产生和组织
• GSE268200 非促有丝分裂 FGF19 mRNA 疗法用于治疗实验性代谢功能障碍相关脂肪肝病 (MASLD)
• GSE179870 多种细胞类型中 m6A 读数的分布图 [RNA-seq]
• GSE318652 P21+TREM2+-衰老巨噬细胞促进炎症衰老和代谢功能障碍相关的脂肪肝疾病[RNAseq_MASHABT263]
• GSE318651 P21+TREM2+-衰老巨噬细胞促进炎症衰老和代谢功能障碍相关的脂肪肝疾病[RNAseq_sgCMPK2]
• GSE318428 整合转录组学和代谢组学分析揭示了两种培养条件下 RPE 细胞不同的能量代谢特征和功能特性
• GSE289238 结肠炎相关癌上皮类器官单独或与匹配的近端结肠成纤维细胞或与匹配的结肠炎相关癌成纤维细胞进行体内共培养
• GSE289177 隐尾蟑螂单细胞核转录组学
• GSE285111 非坏死性凋亡的MLKL功能损伤线粒体并促进造血干细胞衰老
• GSE279473 跨祖先人群肾癌易感性因果关系的遗传图谱和功能探索
• GSE279304 跨祖先人群肾癌易感性因果关系的遗传景观和功能探索 [筛选]
• GSE279303 跨祖先人群肾癌易感性因果关系的遗传景观和功能探索 [CUTnTag]
• GSE279287 跨祖先人群肾癌易感性因果关系的遗传图谱和功能探索 [CROP-seq]