详细内容（前10条）

1. ⭐ GSE294252 炎症和消退程序性髓系回路调控上皮性和间质性三阴性乳腺癌的治疗耐药性

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、inflammation、resistance
• 📝 描述：Contributors : Liqun Yu ; Xiang ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSingle-cell analysis of human triple-negative breast cancer revealed heterogeneous macrophage populations with opposing phenotypes—pro-inflammatory and pro-resolution of inflammation. Paradoxically, both subsets accumulated in therapy-refractory residual tumors but showed inverse correlations across patients, suggesting mutually exclusive resistance mechanisms. Inflammatory macrophages localized preferentially to epithelial-like tumors, whereas pro-resolution macrophages were enriched in mesenchymal-like tumors. Mouse models faithfully recapitulated these patterns. After chemo-immunotherapy, mesenchymal-like tumors expanded pro-resolution macrophages through phagocytosis/efferocytosis, ω-3 fatty-acid uptake, and resolvin production. Macrophage-secreted C1q emerged as a principal antagonist of T-cell function by targeting mitochondria and inducing metabolic dysfunction. By contrast, epithelial-like tumors accumulated inflammatory macrophages and neutrophils that produced prostaglandins via ω-6 fatty-acid pathways. Knocking down ELOVL5—an elongase involved in ω-3 and ω-6 metabolism—mitigated both neutrophil- and macrophage-mediated immunosuppression. These distinct axes, driven by dysregulated inflammation and resolution programs, converged to undermine therapy-induced immunosurveillance; however, targeting their shared upstream regulators may overcome these resistance mechanisms.
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2. ⭐ GSE318353 单细胞转录组分析揭示了黑色素瘤中 miR-18a 介导的靶向治疗和免疫治疗耐药性的异质性

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:immuno(logy|therapy|suppression)、resistance、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn melanoma, the knockout of miR-18a not only leads to resistance to monotherapy with BRAF inhibitors but also to combined therapy with BRAF and MEK inhibitors. This resistance is achieved by promoting the expression of YAP in the Hippo pathway. Additionally, the knockout of miR-18a results in high expression of THBS1-CD47, which causes T cell tolerance. miR-18a is regulated by hnRNPA1, and patients with low expression of miR-18a and hnRNPA1 have poorer Event-Free Survival (EFS).
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3. ⭐ GSE315587 原位空间转录组学揭示了角膜缘干细胞微环境和眼表上皮的新标志物

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Lamia Nureen ; Antonietta Salerno ; Orazio Vittorio ; Nick Di GirolamoSeries Type : OtherOrganism : Mus musculusThe mammalian cornea is endowed with stem cells (SCs) that have lifelong regenerative activity for its stratified epithelium. The niche for these cells is the limbus, and damage to this microstructure or its SCs results in a blinding disease known as limbal stem cell deficiency (LSCD). Despite the numerous studies that employ single-cell RNA sequencing, the identity of these cells remains an enigma principally because their spatial positioning is lost upon dissociation from their repository, and their gene expression is altered upon exposure to physical and chemical stressors during procurement. Herein, these adversities were avoided by conducting spatial transcriptomic screening directly on corneal, limbal and conjunctival tissues. Differentially expressed genes in the limbus included Krt16 and Nkiras1 among 6 others, which play a role in maintaining stemness, epithelial organization, immune surveillance, and tumor suppression. Krt16 was dynamically expressed in the developing limbus, correlated with slow-cycling label-retaining limbal epithelia and was induced during corneal injury; observations consistent with marking functional SCs. Additionally, we established Nkiras1 as a novel maker of neutrophils in the limbal SC niche, and Alox12e as a specific marker of conjunctival epithelia. Because current gold standard treatments for LSCD include SC transplantation, our data will inform future studies in delivering a more reliable standard therapy which incorporates an identifiable SC population to improve clinical outcomes.
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4. ⭐ GSE288888 海马硬化与正常海马的空间转录组学、组织病理学和磁共振亚区分割比较：一项概念验证研究

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Samuel Guzman ; Ken Jones ; Saman HazanySeries Type : OtherOrganism : Homo sapiensDespite recent major advances in work-up and treatment of Drug Resistant Mesial Temporal Lobe Epilepsy (MTLE) with Hippocampal Sclerosis (HS), the underlying pathophysiology of this disease remains elusive. Depending on the type of HS, as defined by the histopathologic International League Against Epilepsy (ILAE), studies report variable post-surgical seizure freedom durations, with diverse underlying pathophysiology and gene expression. We here describe our initial experience with incorporating spatial transcriptomics along with Magnetic Resonance Imaging (MRI) and histopathology in a patient with Drug Resistant MTLE and HS compared to the hippocampus of an age-sex matched control case. Cases were assessed by MRI hippocampal subfields segmentation, neuropathology, and spatial transcriptomics to identify candidate genes that play a role in the biology of HS. A significant finding was a partial inhibition of the methyl-CpG-binding protein 2 (MeCP2) gene in all subfields of the hippocampus, which is important for neuronal survival and differentiation. Together, our results have shown the feasibility of hippocampal subfield segmentation on MRI and direct correlation with histology and spatial transcriptomics to identify key areas of gene dysregulation in epilepsy. These novel methods can be used to implement personalized medicine and discovery of new drug targets.
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5. ⭐ GSE288394 去分化驱动的致癌干性促进肠上皮细胞维持肿瘤的适应性

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、regex:onco(logy|logist|gene|genic)、regex:intestin(e|al)
• 📝 描述：Contributors : Kylee Zgeib ; Thompson Hui ; Atharva Inamdar ; Ansu Perekatt ; Simon Garcia ; Binfeng Lu ; Crysta Lim ; Shima Nejati ; Amartya Singh ; Zahra Hashemi ; Dahlia Matouba ; Christina LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIntestinal tumors can arise either from mutations sustained in Lgr5⁺ stem cells (bottom-up) or through dedifferentiation of lineage-committed epithelial cells (top-down). Using a Smad4 loss-of-function and β-catenin gain-of-function mutant mouse model, we show that dedifferentiation-derived stem cells sustain tumorigenesis more effectively than mutant Lgr5⁺ cells in the endogenous crypts. Bulk and single-cell RNA sequencing revealed transcriptional reprogramming and the emergence of oncogenic villus-derived stem-like cells. Complementary in vivo and organoid studies suggest that dedifferentiation-derived oncogenic stem cells possess superior tumor-initiating and sustaining capacity
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6. ⭐ GSE288393 sc-RNA-seq 研究揭示了去分化肠上皮细胞的致癌重编程为异质干细胞状态 [数据集 1]

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:onco(logy|logist|gene|genic)、RNA-seq、regex:intestin(e|al)
• 📝 描述：Contributors : Kylee Zgeib ; Thompson Hui ; Atharva Inamdar ; Ansu Perekatt ; Crystal Lim ; Shima Nejati ; Binfeng Lu ; Simon Garcia ; Zahra Hashemi ; Amartya Sigh ; Dahlia Matouba ; Christina LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIntestinal tumors can arise either from mutations sustained in Lgr5⁺ stem cells (bottom-up) or through dedifferentiation of lineage-committed epithelial cells (top-down). Using a Smad4 loss-of-function and β-catenin gain-of-function mutant mouse model, we show that dedifferentiation-derived stem cells sustain tumorigenesis more effectively than mutant Lgr5⁺ cells in the endogenous crypts. Bulk and single-cell RNA sequencing revealed transcriptional reprogramming and the emergence of oncogenic villus-derived stem-like cells. Complementary in vivo and organoid studies suggest that dedifferentiation-derived oncogenic stem cells possess superior tumor-initiating and sustaining capacity
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7. GSE313961 内皮素和CD44介导的异型信号传导介导肿瘤-基质串扰并促进肝细胞癌的恶性进展

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、carcinoma
• 📝 描述：Contributors : Woobeen Jo ; Chanho Park ; Min Kim ; Chu-Sook Kim ; Jungsun Yoo ; Sahee Kim ; Jiseon Shin ; Hyun-Woo Rhee ; Jiyoung ParkSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis study profiles gene expression changes in the human SK-Hep1 cell line, a mesenchymal-like liver-derived cell model, under conditions of differential sorafenib responsiveness. Bulk RNA sequencing was performed on sorafenib-sensitive (SS) and sorafenib-resistant (SR) cells treated with control siRNA (siCtrl), COL6A3 siRNA (siCOL6A3), or siCOL6A3 combined with recombinent endotrophin (rhETP). The data were generated to characterize transcriptional patterns associated with altered proliferation-and survival-related phenotypes across defined experimental conditions.
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8. GSE303197 Dynamic Antigen Expression and Intrinsic CTL Resistance in HIV-1 Reservoir Clones

• ✍️ 作者：未知作者
• 🏷️ 关键词：antigen、resistance
• 📝 描述：Contributors : Richard B Jones ; Alberto Herrera ; Paul ZumboSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensReservoirs of clonally expanded CD4+ T-cells harboring rebound-competent HIV-1 proviruses persist lifelong during ART. Latency is considered the principal barrier to viral eradication and has resisted pharmacological reversal, yet it appears that sustained immune pressure may still erode reservoirs. Recent advances have yielded glimpses into these exceptionally rare reservoir-harboring cells, implicating intrinsic pro-survival properties in their persistence. Here, we isolate and characterize populations of authentic reservoir clones (ARCs) that robustly proliferate and accumulate while producing infectious virus, without overtly succumbing to viral cytopathic effects. At any given moment, only small fractions of ARCs expressed HIV-1 proteins, a state remarkably unperturbed by potent TCR stimulation or latency-reversing agents. Nevertheless, sustained co-culture with cytotoxic T-lymphocytes (CTL) revealed extensive time-integrated antigenic vulnerability, culling clonal expansion of some ARCs by >90%. Notably, one regulatory T-cell ARC displayed pronounced cell-intrinsic resistance to CTL – a longstanding hypothesis we now directly demonstrate – linked to low oxidative stress and reversed with desferoxamine, a hypoxic stress inducer and FDA-approved therapeutic. Overall, we provide novel insights into the vulnerabilities of reservoir clones to potent, sustained CTL pressure and highlight intrinsic resistance pathways as actionable therapeutic targets, opening opportunities for advancing immune-based HIV-1 cure strategies.
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9. GSE296017 癌症、地塞米松和衰老引起的骨骼肌萎缩主要由不同的翻译后修饰引起。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、aging
• 📝 描述：Contributors : Anna Stephan ; Yong-Dong Wang ; Fabio DemontisSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGene expression changes induced by LrpprcP27A vs. LrpprcWT in skeletal muscle
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10. GSE217516 支气管扩张患者支气管肺泡灌洗液细胞的单细胞RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell
• 📝 描述：Contributors : Elza Evren ; Tim WillingerSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBronchiectasis is a lung disease characterized by irreversible dilation of the large airways (bronchodilatation), which often causes recurrent airway infection and non-resolving inflammation. In this study, we performed single-cell RNA-sequencing (scRNA-seq) to determine the heterogeneity of immune cells in the airways of bronchiectasis patients.
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1. 科学家刚刚绘制出了驱动癌症生长的突变图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Researchers have created the first complete map showing how hundreds of mutations in a key cancer gene affect tumor growth. By testing every possible mutation in a critical hotspot, they found that some changes barely boost cancer signals, while others supercharge them. When matched against real patient data, the map accurately predicted cancer behavior across tissues.
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2. 新型鼻腔疫苗对H5N1禽流感显示出强大的保护作用

• ✍️ 作者：未知作者
• 🏷️ 关键词：vaccine
• 📝 描述：As bird flu continues to circulate in animals and spill over into humans, researchers are racing to stop it before it adapts to spread widely between people. A new nasal spray vaccine showed strong protection against H5N1 in animal tests, outperforming traditional flu shots. Because it targets the nose and lungs, it may prevent infection at the earliest stage.
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• GSE311781 研究发现，局灶性白质病变可导致灰质炎症、神经元功能障碍和突触丢失。
• GSE318581 转移性激素敏感性前列腺癌上皮异质性的单细胞转录组图谱
• GSE318354 miR-18a CRISPR 敲除黑色素瘤的转录组分析鉴定出与治疗耐药性和 T 细胞杀伤相关的基因
• GSE289920 定制基于 CRISPR/Cas 的表观基因组编辑器以实现可编程染色质酰化和降低细胞毒性 [靶向扩增子测序 II]
• GSE289257 定制基于 CRISPR/Cas 的表观基因组编辑器以实现可编程染色质酰化和降低细胞毒性 [RNA-Seq,2]
• GSE288948 巨噬细胞 Smad1 导致梗死后功能障碍，抑制血管生成趋化因子表达，并刺激细胞外基质重塑
• GSE255561 定制基于 CRISPR/Cas 的表观基因组编辑器以实现可编程染色质酰化和降低细胞毒性 [RNA-Seq]
• GSE255195 定制基于 CRISPR/Cas 的表观基因组编辑器以实现可编程染色质酰化和降低细胞毒性 [靶向扩增子测序]
• GSE318477 秀丽隐杆线虫滞育发育过程中基因表达的通路特异性通道化和可塑性
• GSE303395 氧固醇受体 GPR183 感知代谢微环境信号，促进单核细胞分化为组织驻留肺巨噬细胞
• GSE303039 Elevated levels of Ube2g1 in hematopoietic stem cells lead to segmental aging of the hematopoietic system
• GSE298718 解码胶质母细胞瘤侵袭：肿瘤聚集体中环境和细胞周期效应的单细胞分析
• GSE298406 神经发生阶段肠神经系统祖细胞染色质可及性分析
• 对8周龄KPPC WT、MAP3K7ff KPPC和MAP3K7fwt KPPC小鼠胰腺肿瘤进行GSE297618单细胞RNA测序分析
• GSE318560 神经退行性痴呆症（阿尔茨海默病、唐氏综合征和帕金森病）海马体的共同致病机制
• GSE306150 主动脉炎样本的空间基因表达谱分析。
• GSE300299 基孔肯雅病毒持续存在于关节相关巨噬细胞中，并促进慢性疾病的发生。[scRNA-seq]
• GSE318613 恒河猴对疫苗的适应性免疫反应反映了首次接触时的社会地位
• GSE318538 KIFC1敲低对H146小细胞肺癌细胞基因表达的影响
• 基于 CRISPR 筛选的 GSE318501 miRNA-18a 可调控黑色素瘤中的靶向治疗耐药性和 T 细胞杀伤作用
• GSE318414 日光性雀斑中脂质代谢紊乱：多系统水平分析揭示膜不稳定性和能量稳态破坏
• GSE318342 Snail1 通过 EGR1、FOXO1 和 CEPBg 诱导增殖抑制，为靶向凋亡和细胞衰老通路创造了脆弱性 [ChIP-Seq]
• GSE318340 Snail1 通过 EGR1、FOXO1 和 CEPBg 诱导增殖抑制，为靶向凋亡和细胞衰老通路创造了脆弱性 [RNA-Seq]
• GSE318318 BAF60C在2型糖尿病中的作用——单细胞RNA测序
• GSE318308 一项 II 期概念验证研究，评估度伐利尤单抗和西地尼布联合或不联合奥拉帕尼治疗复发性卵巢癌的疗效
• GSE318305 甘氨酸通过 GCN2/AKT 轴促进心肌细胞增殖和心脏再生
• GSE317978 BAF60C在2型糖尿病中的作用——RNA测序
• GSE317708 BAF60C 在 2 型糖尿病中的作用 ——ATAC-seq
• GSE317461 CUET 处理的甲状腺 8505 细胞的特征分析 (RNA-Seq)
• GSE311046 神经生长因子 (NGF) 驱动 ILC2 的促肿瘤功能 - 人类 TRKA+ 和 TRKA- ILC2 的批量 RNA 测序
• GSE291324 对低葡萄糖或 MAPKAPK2 表达缺失的 HCT116 细胞进行转录组分析。
• GSE291147 利用 PerturbFate 绘制黑色素瘤耐药性的趋同调控因子图谱
• GSE289256 鳞状细胞肺癌的多组学图谱
• GSE289032 G1-APOL1 骨髓嵌合 B6 小鼠和完全 MHC 不匹配的异位心脏移植排斥反应（BALBc-to-B6 模型）
• GSE289030 BAC转基因G1-和G0-APOL1小鼠CD8+T细胞在体外刺激后的转录组。
• GSE282078 CAR-M2水凝胶治疗对肾纤维化小鼠纤维化和内皮血管生成等相关基因表达的影响（批量RNA测序）
• GSE255194 定制基于 CRISPR/Cas 的表观基因组编辑器以实现可编程染色质酰化和降低细胞毒性 [CUT&RUN]
• GSE248745 通过原位 APEX2 驱动的生物素化建立原始卵泡阶段卵母细胞蛋白质组及顺铂诱导的变化
• 利用新一代测序技术评估AhR对小鼠肝脏的影响（GSE173800）