详细内容（前10条）

1. ⭐ 利用单细胞RNA测序技术在ST36穴位中鉴定出S100A8/S100A9-Toll样受体4信号通路，该通路介导针灸镇痛。

• ✍️ 作者：未知作者
• 🏷️ 关键词：Toll-like receptor、sequencing、single-cell
• 📝 描述：Contributors : Baomin Dou ; Ruping Yuan ; Zezhi Fan ; Jiangshan Wang ; Yangyang Liu ; Shenjun Wang ; Xiaowei Lin ; Yanwei Li ; Kaifang Yao ; Zhihan Chen ; Zhifang Xu ; Gang Hu ; Yi GuoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPain is a primary indication for acupuncture, with ST36 shown to effectively treat various types and locations of pain. Acupoints serve as pivotal starting points for acupuncture’s therapeutic effects. However, how acupuncture stimulation alters the microenvironment at the ST36 acupoint to treat different pain conditions remains unclear. We investigated the analgesic effects of ST36 acupuncture in mice with adjuvant arthritis, identifying fibroblast and macrophage clusters as the main responsive populations at ST36 through single-cell sequencing analysis. These populations expressed high levels of S100 calcium-binding protein a8 (S100a8), S100a9, and Toll-like receptor 4 (TLR4) on both cells and afferent fibers. Furthermore, acupuncture at ST36 activated A and C afferent nerves. Intriguingly, acupoint injection of an S100a8/S100a9-TLR4 inhibitor reversed the excitation of A and C afferent nerves and diminished the analgesic effect of acupuncture. These findings offer novel insights into the ST36 acupoint microenvironment modulated by acupuncture and underscore the critical role of S100a8/S100a9 in initiating acupuncture-induced analgesia at ST36. The study identifies S100a8/S100a9 as a potential regulatory target for modulating acupuncture effects at acupoints, providing a molecular framework for standardized acupuncture interventions and their clinical applications.
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2. GSE289591 肠道本土微生物调节神经细胞状态和神经退行性疾病易感性 [单菌定植_RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Lisa Blackmer-Raynolds ; Lyndsey D Lipson ; Anna Kozlov ; Aimee Yang ; Emily J Hill ; Maureen M Sampson ; Adam M Hamilton ; Isabel Fraccaroli ; Sean D Kelly ; Pankaj Chopra ; Jianjun Chang ; Steven A Sloan ; Timothy R SampsonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe native microbiome influences a plethora of host processes, including neuroimmune function. However, the consequences of individual gut microbes on neuroinflammatory tone remain largely unexplored. In the present dataset, we investigated the consequences of four bacterial type strains representing prevalent genera within the mammalian gut microbiome (Bacteroides thetaiotaomicron (B. theta), Clostridium celatum, Lactobacillus johnsonii, and Escherichia coli) on the transcriptional profile of CD11b+ brain myeloid cells. After 2 weeks of mono-colonization with the bacteria species of interest, it was found that each bacterial type had distinct effects on brain myeloid cell gene expression, highlighting the bacterial dependent consequences of the microbiome for neuroinflammatory outcomes.
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3. GSE289590 肠道固有微生物调节神经细胞状态和神经退行性疾病易感性 [5xFAD_CD11b+_Bulk_RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Lisa Blackmer-Raynolds ; Lyndsey Lipson ; Anna Kozlov ; Aimee Yang ; Emily J Hill ; Maureen M Sampson ; Adam M Hamilton ; Pankaj Chopra ; Jianjun Chang ; Steven A Sloan ; Timothy R SampsonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe native microbiome influences a plethora of host processes, including neurological function. We have shown that colonization by Escherichia coli induces a distinct adaptive immune and neurogenerative disease-associated cell state in wildtype mice, suggesting increased disease susceptibility. Further, E. coli exposure in the 5xFAD mouse model results in exacerbated cognitive decline and amyloid pathology, demonstrating its sufficiency to worsen Alzheimer’s disease-relevant outcomes. Here, we performed bulk RNA-seq of CD11b+ brain myeloid cells from E. coli or vehicle treated 5xFAD mice to understand how E. coli modulates neuroimmune responses in the context of amyloid pathology. We demonstrate that E. coli exposed animals have a subtle decrease in genes associated with the disease associated microglia phenotype after 1 month of enrichment, perhaps helping to explain why these animals displayed worsened outcomes.
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4. GSE289589 肠道本土微生物调节神经细胞状态和神经退行性疾病易感性 [snRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Lisa Blackmer-Raynolds ; Lyndsey D Lipson ; Anna Kozlov ; Aimee Yang ; Emily J Hill ; Maureen M Sampson ; Adam M Hamilton ; Isabel Fraccaroli ; Sean D Kelly ; Pankaj Chopra ; Jianjun Chang ; Steven A Sloan ; Timothy R SampsonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe native microbiome influences a plethora of host processes, including neurological function. However, its impacts on diverse brain cell types remains poorly understood. Here, we performed single nucleus RNA sequencing on hippocampi from wildtype, germ-free mice and reveal the microbiome-dependent transcriptional landscape across all major neural cell types. We found conserved impacts on key adaptive immune and neurodegenerative transcriptional pathways, underscoring the microbiome’s contributions to disease-relevant processes. In addition, mono-colonization with Escherichia coli was found to induce a distinct adaptive immune and neurogenerative disease-associated cell state, suggesting increased disease susceptibility.
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5. GSE318148 饮食、寄生虫感染和抗生素治疗对小鼠肠道转录组的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：transcriptome、regex:intestin(e|al)
• 📝 描述：Contributor : Andrew R WilliamsSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDiets rich in whole grains and fermentable fibers may promote gut health, whilst processed foods can lead to inflammation and increased susceptibility to pathogens. Here, we show that mice infected with the roundworm Heligmosomoides polygyrus exhibited increased resistance to infection when fed fiber-deprived semi-synthetic diets (SSD), relative to mice fed fibre-rich isoenergetic chow. SSD-fed mice had augmented transcription of type-2 immune-related genes during infection, and worm fitness (fecundity) was reduced. Enhanced anti-helminth immunity in SSD-fed mice was associated with suppression of gene pathways related to xenobiotic metabolism and aryl-hydrocarbon receptor (AhR) activation in the gut mucosa. However, inclusion of synthetic AhR agonists in SSD did not recapitulate the effect of chow. Moreover, whilst diet composition regulated host gut microbiota (GM) changes in response to H. polygyrus, infection resistance in SSD-fed mice was independent of the GM as antibiotic depletion of the GM failed to attenuate diet-mediated effects on parasite fecundity. Thus, in contrast to well-known health-promoting effects of whole-grain diets, synthetic diet components directly enhance resistance to helminth infection in the small intestine. These findings emphasize the role of the physicochemical structure of diet in regulating host defense at mucosal surfaces.
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6. GSE318134 低剂量 TNF-α 通过 TRAF2–FASN 轴驱动胶质母细胞瘤的恶性进展和脂质代谢 [RNA-Seq TRAF2-KD]

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolism、RNA-seq
• 📝 描述：Contributors : Maorong Cai ; Yang Liu ; Xinyu Mao ; Jiantong JiaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensLipid metabolism plays a critical role in the progression of cancers, including glioblastoma (GBM). Tumor necrosis factor-α (TNF-α) exhibits a dual role in the tumor microenvironment: at high concentrations, it induces cell death and exerts anti-tumor effects, while at low doses, it demonstrates pro-tumorigenic activity. This study investigates the regulatory effects of low-dose TNF-α on the malignant behavior and lipid metabolism of GBM. The results show that low-dose TNF-α (10 ng/mL) significantly promotes GBM cell Malignant phenotypes and lipid droplet accumulation via the TNFR signaling pathway, a process dependent on its downstream adaptor protein, Tumor necrosis factor receptor-associated factor 2 (TRAF2). Mechanistically, TRAF2 interacts with Fatty acid synthase (FASN) through its TRAF domain and, serving as an E3 ubiquitin ligase, leverages its RING domain to mediate K63-linked polyubiquitination of FASN. This enhances FASN protein stability, promotes lipid synthesis, and ultimately drives tumor progression. Furthermore, through virtual screening, the small-molecule compound Jionoside B1 was identified to target the RING domain of TRAF2, effectively inhibiting K63-linked ubiquitination of FASN and disrupting the TRAF2–FASN interaction and protein accumulation. In both in vitro and in vivo experiments, Jionoside B1 significantly suppressed lipid synthesis and attenuated tumor growth. This study systematically elucidates the mechanism by which low-dose TNF-α regulates lipid metabolism and promotes GBM malignant progression via the TRAF2–FASN axis, providing not only new insights into the “double-edged sword” role of TNF-α in the tumor microenvironment but also a potential novel therapeutic strategy for targeting this pathway in GBM treatment.
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7. GSE307499 Vγ1γδT细胞在自体肺癌小鼠模型中引导气道巨噬细胞产生促纤维化反应。[RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、RNA-seq
• 📝 描述：Contributors : Ximena L. Raffo-Iraolagoitia ; Amanda J. McFarlane ; Sarah Laing ; Ryan Corbyn ; Lindsey W. G. Arnott ; Frédéric Fercoq ; Lynn McGarry ; Judith Secklehner ; Marco De Donatis ; John B. G. Mackey ; Bjorn Kruspig ; Robert Wiesheu ; Ya-Ching Hsieh ; Robin Shaw ; Kai Rakovic ; John Le Quesne ; Graeme Clark ; Colin Nixon ; Crispin Miller ; Kristina Kirschner ; Calum C. Bain ; Daniel J. Murphy ; Seth B. Coffelt ; Leo M. CarlinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGamma-delta T cells are important for host defence at the respiratory mucosa, acting directly or through interactions with other cells. However, how Gamma-Delta T cells influence other immune cells in the lung remains unclear. Using a genetically engineered mouse model of lung cancer, we show that tumours drive expansion of both CD27+ and CD27— Gamma-Delta T cells. Advanced microscopy techniques indicated that CD27— Gamma-Delta T cells are enriched in tumours, while CD27+ Gamma-Delta T cells are more prone to interact with macrophages in tumour-associated adventitial cuffs. SiglecFlow pro-fibrotic airway macrophages were more prevalent in lung tumour-bearing mice than tumour-free mice. This pro-fibrotic subset was reduced in lungs when the cancer model was crossed to Tcrd knockout mice or treated with V Gamma1-depleting antibodies, but not in TcrgV4/6 knockout mice. Thus, our findings implicate V Gamma1 Gamma-Delta T cells in driving tumour-associated airway macrophage functional imprinting. Determining the translatability to human health may offer new avenues for refining patient management and immunotherapeutic strategies.
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8. GSE307497 Vγ1γδT细胞在自体肺癌小鼠模型中引导气道巨噬细胞产生促纤维化反应[scRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、scRNA
• 📝 描述：Contributors : Ximena L. Raffo-Iraolagoitia ; Amanda J. McFarlane ; Sarah Laing ; Ryan Corbyn ; Lindsey W. G. Arnott ; Frédéric Fercoq ; Lynn McGarry ; Judith Secklehner ; Marco De Donatis ; John B. G. Mackey ; Bjorn Kruspig ; Robert Wiesheu ; Ya-Ching Hsieh ; Robin Shaw ; Kai Rakovic ; John Le Quesne ; Graeme Clark ; Colin Nixon ; Crispin Miller ; Kristina Kirschner ; Calum C. Bain ; Daniel J. Murphy ; Seth B. Coffelt ; Leo M. CarlinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGamma-delta T cells are important for host defence at the respiratory mucosa, acting directly or through interactions with other cells. However, how Gamma-Delta T cells influence other immune cells in the lung remains unclear. Using a genetically engineered mouse model of lung cancer, we show that tumours drive expansion of both CD27+ and CD27— Gamma-Delta T cells. Advanced microscopy techniques indicated that CD27— Gamma-Delta T cells are enriched in tumours, while CD27+ Gamma-Delta T cells are more prone to interact with macrophages in tumour-associated adventitial cuffs. SiglecFlow pro-fibrotic airway macrophages were more prevalent in lung tumour-bearing mice than tumour-free mice. This pro-fibrotic subset was reduced in lungs when the cancer model was crossed to Tcrd knockout mice or treated with V Gamma1-depleting antibodies, but not in TcrgV4/6 knockout mice. Thus, our findings implicate V Gamma1 Gamma-Delta T cells in driving tumour-associated airway macrophage functional imprinting. Determining the translatability to human health may offer new avenues for refining patient management and immunotherapeutic strategies.
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9. GSE315430 单细胞转录组分析揭示骨髓微环境相关细胞的年龄相关性改变

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell
• 📝 描述：Contributors : Kei Ishibashi ; Mitsuaki Ono ; Ikue Tosa ; Ziyi Wang ; Wakana Kitagawa ; Anh T Dang ; Kentaro Akiyama ; Tomoko Yonezawa ; Toshitaka Oohashi ; Takuo KubokiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusHematopoietic stem cells (HSCs) depend on the bone marrow (BM) niche for self-renewal and differentiation. Within this niche, CXCL12-abundant reticular (CAR) cells are key regulators of hematopoiesis and bone homeostasis. Single-cell RNA sequencing of adult and old mice revealed that old Adipo-CAR cells form a distinct cluster with reduced expression of ossification- and stem cell maintenance–related genes. RNA velocity and pseudotime analyses indicated impaired osteogenic differentiation, which was confirmed by in vitro assays showing diminished osteogenesis and increased adipogenesis. Structural and immunohistochemical analyses further demonstrated sinusoidal and endothelial abnormalities with age. These results suggest that age-related changes in Adipo-CAR cells and the sinusoidal niche underlie defective osteogenesis and hematopoietic decline.
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10. GSE311820 对来自食管次全切除术的人类食管活检组织进行食管细胞单细胞 mRNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：Contributors : Benjamin Beck ; Louison DescampeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn our study, we used single-cell RNA sequencing to characterize epithelial cell populations in the human esophagus in control condition compared to Barrett esophagus condition.
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1. RNA测序揭示脑转移瘤的不同分子亚型

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：By combining multi-omics and RNA sequencing across more than 1,000 samples, researchers define distinct brain metastasis subtypes that reflect the brain microenvironment and…
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2. 一种隐秘的细胞过程可能驱动衰老和疾病

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：As we age, our cells don’t just wear down—they reorganize. Researchers found that cells actively remodel a key structure called the endoplasmic reticulum, reducing protein-producing regions while preserving fat-related ones. This process, driven by ER-phagy, is tied to lifespan and healthy aging. Because these changes happen early, they could help trigger later disease—or offer a chance to stop it.
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• GSE311819：野生型 (WT) 和 Krt5-CreERT2:R26SmoM2/EYFP (K5:Smo) 小鼠在服用他莫昔芬 5 天后食管细胞的单细胞 mRNA 测序
• 对注射他莫昔芬 5 天后从 K5:Smo 小鼠食管类器官中提取的 GSE294847 mRNA 进行测序。
• GSE294082 糖皮质激素抑制早期肺部炎症并损害非人灵长类动物对 SARS-CoV-2 的控制。
• 对未经处理和经布洛芬处理的 Krt5-CreERT2:R26SmoM2/EYFP (K5:Smo) 小鼠食管细胞进行 GSE291095 mRNA 测序
• 对源自野生型小鼠的食管类器官进行GSE291094 mRNA测序
• GSE291090 对未经处理和经布洛芬处理的 Krt5-CreERT2:R26SmoM2/EYFP (K5:Smo) 小鼠食管细胞进行单细胞 mRNA 测序
• GSE314684 对照组与 AAV-OPDM 小鼠模型的单肌核 RNA 测序
• GSE303077：ALS/FTLD-TDP rNLS8模型小鼠全脑细胞诱导后4周的单核RNA测序。
• GSE281126：ALS小鼠模型中全脑细胞的单核RNA测序
• GSE246189 H3K115 标记调控位点的脆弱核小体 [ATAC-seq]
• GSE246186 H3K115 标记调控位点的脆弱核小体 [ChIP-seq]
• GSE317610 角鲨烯藿烯环化酶在烟曲霉唑类耐药性和毒力中的作用
• GSE316435 LncRNA AC098613.1 通过 CDC5L/ADAP1/NRD1 轴促进急性髓系白血病细胞分化
• GSE318211 RNA-seq 分析了用重组 sST2 和血管紧张素 II 处理的人主动脉平滑肌细胞。
• GSE318210 血管紧张素II诱导的主动脉疾病模型中sST2敲除小鼠主动脉的RNA测序
• GSE318173 帕金森病全血表观基因组关联研究、荟萃分析和多评分分析
• GSE318064 低剂量 TNF-α 通过 TRAF2–FASN 轴驱动胶质母细胞瘤的恶性进展和脂质代谢。
• GSE305468 早期 cAMP 信号传导调控盘基网柄菌发育过程中的单细胞同步性
• GSE300385 三阴性乳腺癌亚型生物标志物的发现与评估揭示了患者分层和靶向治疗策略
• GSE288704 对未突变散发性 ALS 患者外周血的全转录组分析揭示了表型特异性基因表达特征
• GSE288699 小鼠假胚胎缺乏所有 Hox 功能，存在多种缺陷 [scRNA-seq]
• GSE288698 小鼠假胚胎缺乏所有 Hox 功能，存在多种缺陷 [RNA-seq]
• GSE288696 小鼠假胚胎缺乏所有 Hox 功能，存在多种缺陷 [ATAC-seq]
• GSE288681 小鼠交感神经系统激活通过去甲肾上腺素-β2-肾上腺素能受体信号通路驱动干眼症的发生
• GSE240605 杂合子或纯合子 Myo7a Shaker 突变小鼠的耳蜗组织 [RNA-seq]