详细内容（前10条）

1. ⭐ GSE308859 整合空间转录组学和单细胞 RNA 测序揭示 Lars2 介导的小鼠横向主动脉缩窄 (TAC) 模型中心肌重塑的时空动态

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell、spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Hanwen Ni ; Feng Liang ; Huanhuan Huo ; Xuechao Feng ; Ben HeSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusMulti-omics approaches have emerged as a cornerstone in medical biology, offering an integrative perspective on tissue and cellular composition, gene expression patterns, functional states, and metabolic dynamics. In this study, we employed multi-omics methodologies, combining spatial transcriptomics (ST) and single-cell RNA sequencing (scRNA-seq), to perform large-scale bioinformatics analysis on heart samples from Transverse Aortic Constriction (TAC) mouse models at various stages of disease progression. The aim was to investigate the spatial and temporal characteristics of cellular distribution, gene composition, and associated functional changes within the myocardium in response to pressure overload. For the first time, the pathophysiology of myocardial hypertrophy in this model is described through the integrated lens of spatial and single-cell transcriptomics. The spatial transcriptomics analysis revealed a uniform cellular distribution across the heart tissue in TAC mice at different disease stages. However, substantial shifts in cellular composition were observed, with the most significant alterations occurring in cluster 1, which primarily involved changes in fibroblasts and macrophages. Single-cell RNA sequencing further demonstrated that the most notable changes in cellular composition occurred at the TAC-4W stage, characterized by a reduction in fibroblast and macrophage populations and an increase in immune cell subsets, such as neutrophils and T cells. By TAC-6W, the cellular composition returned to a state comparable to that observed at TAC-2W. Further analysis integrating both spatial and single-cell transcriptomes highlighted cluster 1 as the site of the most prominent microenvironmental changes, predominantly involving T cells and macrophages. Notably, the genes Lef1, Ccr7, Sell, and Lars2 were identified as significantly differentially expressed in these cells—an observation not previously reported. In particular, the expression of Lars2 exhibited dynamic modulation throughout disease progression. While its expression at TAC-2W did not differ significantly from controls, Lars2 expression peaked at TAC-4W, only to decline at TAC-6W. This study provides a comprehensive characterization of myocardial hypertrophy in the TAC mouse model, leveraging the power of spatial trans…
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2. ⭐ GSE302765 肿瘤特异性树突状细胞和抗体疗法在肿瘤内的整合驱动先天性和适应性抗癌免疫 [PanCancer_IO360_NanoString]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、dendritic cell、antibody
• 📝 描述：Contributors : Saurabh Garg ; Brian CzernieckiSeries Type : Expression profiling by arrayOrganism : Mus musculusComparison of gene expression profiles of HER2+ murine mammary carcinoma treated with HER2 DC1, anti-Sema4D, and combination of both HER2 DC1 and anti-Sema4D therapy
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3. ⭐ GSE302764 肿瘤特异性树突状细胞和抗体疗法在肿瘤内的整合驱动先天性和适应性抗癌免疫 [Immunology_NanoString]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、dendritic cell、antibody
• 📝 描述：Contributors : Saurabh Garg ; Brian CzernieckiSeries Type : Expression profiling by arrayOrganism : Mus musculusComparison of gene expression profiles of HER2+ murine mammary carcinoma treated with HER2 DC1, anti-Sema4D, and combination of both HER2 DC1 and anti-Sema4D therapy
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4. ⭐ GSE302763 肿瘤特异性树突状细胞和抗体疗法在肿瘤内的整合驱动先天性和适应性抗癌免疫 [Myeloid_Innate_Immunity_NanoString]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、dendritic cell、antibody
• 📝 描述：Contributors : Saurabh Garg ; Brian CzernieckiSeries Type : Expression profiling by arrayOrganism : Mus musculusComparison of gene expression profiles of HER2+ murine mammary carcinoma treated with HER2 DC1, anti-Sema4D, and combination of both HER2 DC1 and anti-Sema4D therapy
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5. ⭐ GSE302735 肿瘤特异性树突状细胞和抗体疗法在肿瘤内的整合驱动先天性和适应性抗癌免疫 [Metabolic_Pathways_NanoString]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、dendritic cell、antibody
• 📝 描述：Contributors : Garg Saurabh ; Czerniecki BrianSeries Type : Expression profiling by arrayOrganism : Mus musculusComparison of gene expression profiles of HER2+ murine mammary carcinoma treated with HER2 DC1, anti-Sema4D, and combination of both HER2 DC1 and anti-Sema4D therapy
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6. ⭐ GSE317909 TOX 驱动 TH1 CD4+ T 细胞效应功能、抗肿瘤免疫和自身免疫性疾病的致病性 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、T cell、RNA-seq
• 📝 描述：Contributors : Andrea Schietinger ; Brianna Naizir ; Andrew Scott ; Doron Betel ; Paul ZumboSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusRNA-seq analysis of TOX WT and TOX KO transgenic (TCRSMARTA) CD4 T cells from tdLN of tumor-bearing mice day 11 post transfer
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7. ⭐ GSE301073 不同的肿瘤免疫微环境 (TIME) 特征驱动胶质母细胞瘤中不同的免疫治疗反应

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immune、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Linqian Weng ; Krish Skandha Gopalan ; Gabriele BergersSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusGlioblastoma (GBM) exhibits significant molecular heterogeneity leading to variable treatment responses. Despite multimodal therapies, prognosis remains poor, highlighting the need for personalized approaches targeting the tumor immune microenvironment (TIME). Using single-cell RNA sequencing, multiplex immunohistochemistry, and orthotopic mouse models, we characterized distinct TIME subtypes and evaluated responses to anti-angiogenic immunotherapy and myeloid-targeting approaches. We identified three TIME subtypes: TIME-low (immune-excluded with abnormal vasculature), TIME-med (immune-infiltrated with functional T cells), and TIME-high (heavily infiltrated with immunosuppressive myeloid cells and anergic T cells). TIME-low GBMs responded transiently to anti-angiogenic immunotherapy with immunostimulatory T cell shifts, while anti-angiogenic therapy was ineffective in TIME-high GBMs due to immunosuppressive myeloid cells. CD40 agonist treatment worsened outcomes in TIME-high GBMs by increasing immunosuppressive cells and reducing NK recruitment. Conversely, PI3Kγ/δ inhibition combined with anti-angiogenic immunotherapy modestly extended survival in TIME-high tumors. Our study reveals that GBM subtypes require tailored therapeutic strategies, with TIME classification potentially predicting treatment responses and TIME-high tumors requiring myeloid reprogramming to overcome immunosuppression.
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8. ⭐ GSE273583 转移性黑色素瘤对免疫检查点阻断疗法的先天性和获得性耐药性的综合蛋白质基因组学和空间分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、resistance、spatial
• 📝 描述：Contributors : Shiyou wei ; Kuang Du ; Hongbin Lan ; Zhenyu Yang ; Yulan Deng ; Zhi Wek ; Dennie T Frederick ; Jinho Lee ; Marilyne Labrie ; Tian Tian ; Tabea Moll ; Yeqing Chen ; Ryan J Sullivan ; Gordon Mills ; Genevieve M Boland ; Keith T Flaherty ; Lunxu Liu ; Meenhard Herlyn ; Gao ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWhile a subset of patients with metastatic melanoma achieves durable responses to immune checkpoint blockade (ICB) therapies, the majority ultimately exhibit either innate or acquired resistance to these treatments. However, the molecular mechanisms underlying resistance to ICB therapies remain elusive and are warranted to elucidate. Here, we comprehensively investigated the tumor and tumor immune microenvironment (TIME) of paired pre- and post-treatment tumor specimens from metastatic melanoma patients who were primary or secondary resistance to anti-CTLA-4 and/or anti-PD-1/PD-L1 therapies. Differentially expressed gene (DEG) analysis and single-sample gene set enrichment analysis (ssGSEA) with transcriptomic data identified cell cycle and c-MYC signaling as pathway-based resistance signatures. And weighted gene co-expression network analysis (WGCNA) revealed the activation of a cross-resistance meta-program involving key signaling pathways related to tumor progression in ICB resistant melanoma. Moreover, spatially-resolved, image-based immune monitoring analysis by using NanoString’s digital spatial profiling (DSP) and Cyclic Immunofluorescence (CyCIF) showed infiltration of suppressive immune cells in the tumor microenvironment of melanoma with resistance to ICB therapies. Our study reveals the molecular mechanisms underlying resistance to ICB therapies in patients with metastatic melanoma by conducting such integrated analyses of multi-dimensional data, and provides rationale for salvage therapies that will potentially overcome resistance to ICB therapies.
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9. ⭐ GSE317910 TOX 驱动 TH1 CD4+ T 细胞效应功能、抗肿瘤免疫和自身免疫性疾病的致病性 [CUT&RUN]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、T cell
• 📝 描述：Contributors : Simon Grassmann ; Brianna Naizir ; Paul Zumbo ; Doron Betel ; Andrea SchietingerSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusCUT&RUN analysis of CD4 and CD8 T cells (confirmed to express TOX)
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10. ⭐ GSE317904 TOX 驱动 TH1 CD4+ T 细胞效应功能、抗肿瘤免疫以及自身免疫性疾病的致病性

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、T cell
• 📝 描述：Contributors : Andrea Schietinger ; Brianna Naizir ; Andrew Scott ; Doron Betel ; Paul ZumboSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusATAC-seq analysis of TOX WT and TOX KO transgenic (TCRSMARTA) CD4 T cells from tdLN of tumor-bearing mice at day 11 post transfer
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1. SeekGene推出高通量单细胞DNA甲基化+RNA多组学解决方案

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、methylation
• 📝 描述：SeekGene unveiled a high-throughput single-cell platform that integrates RNA sequencing with DNA methylation and immune profiling..
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2. 科学家发现一种蛋白质，它能修复肠漏并缓解抑郁症。

• ✍️ 作者：未知作者
• 🏷️ 关键词：gut、regex:gut(-?microbiome)?
• 📝 描述：Chronic stress can damage the gut’s protective lining, triggering inflammation that may worsen depression. New research shows that stress lowers levels of a protein called Reelin, which plays a key role in both gut repair and brain health. Remarkably, a single injection restored Reelin levels and produced antidepressant-like effects in preclinical models. The findings hint at a future treatment that targets depression through the gut–brain connection.
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3. 利用分层交叉熵损失改进图谱尺度单细胞注释模型

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell
• 📝 描述：A new approach to RNA sequencing analysis shows that respecting biological cell hierarchies improves single-cell annotation accuracy, especially when models are applied to new…
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• GSE316959 靶细胞上的 MHC I 类分子调节 CD4+ T 细胞介导的免疫
• GSE299087 抗原特异性免疫疗法利用CD4杂交胰岛素肽新表位抑制胰岛移植中CD8 T细胞的分化
• GSE293896 空间转录组学揭示 APOE4 等位基因对路易体痴呆中 α-突触核蛋白聚集和细胞间通讯的影响
• GSE308355 CLK1 通过激活 Hippo/YAP 信号通路促进 MDSC 迁移并重编程结直肠癌肿瘤微环境
• GSE308264：Rett综合征iPS细胞中组蛋白修饰H3K27ac和CTCF转录因子结合的全基因组分析
• GSE246314 HEK293细胞经VPA处理后组蛋白修饰H3K27ac和CTCF转录因子结合的全基因组分析
• GSE245856 HEK293细胞经VPA处理后采用RNA-seq进行全基因组基因表达谱分析
• GSE245848 HEK293细胞经VPA处理后，利用Hi-C进行全基因组染色质相互作用分析
• GSE314572 3型免疫细胞中IL-22基因的顺式调控[RNA-Seq]
• GSE314555 IL-22 基因在 3 型免疫细胞中的顺式调控 [Chip-seq]
• GSE314539 IL-22 基因在 3 型免疫细胞中的顺式调控 [ATAC-seq]
• GSE240148 小鼠体内移植的B2M KO CD40处理的B16肿瘤中肿瘤微环境的转录组分析
• GSE314851 通过 FAP 靶向免疫疗法靶向动脉粥样硬化中受调控的血管平滑肌细胞 [Visium]
• GSE317742 从肿瘤部位迁移到胸腺的树突状细胞的特征分析 [RNA-Seq]
• GSE302512 T细胞衍生的TNF驱动心肌炎，但对检查点免疫疗法的抗肿瘤疗效并非必需
• GSE293887 高级别浆液性卵巢癌微环境的3D五重培养模型转录组学
• GSE318176 对不同 SARS-CoV-2 疫苗接种者外周血的整合表观基因组学和转录组学分析揭示了 TGF-β 介导的耗竭机制
• GSE317457 糖基化GM-CSF扩增B-1b细胞和B-1b浆细胞并使其产生免疫抑制作用
• GSE316800 拉伸通过直接机械效应抑制MMTV-PYMT中的肿瘤生长
• GSE316197 NanoString 乳腺癌 360 表达谱分析，来自 NHBW 和 NHWW 供体的组织学正常乳腺小叶 (BC360)
• GSE299250 L1CAM 通过 NF-κB 激活促进人类子宫内膜癌的发生
• GSE294707 消除 DGCR8 表达对胚胎畸胎瘤 PA-1 细胞稳态 RNA 水平的影响 [RNA-Seq]
• GSE314554 3 型免疫细胞中 IL-22 基因的顺式调控 [ATAC-seq_Mnk3]
• GSE314532 3型免疫细胞中IL-22基因的顺式调控
• GSE314278 利用新型通用γ链细胞因子融合分子治疗生成持久性HIV CAR-TSCM细胞
• GSE314231 化疗诱导的反应性髓系造血导致小鼠和人类免疫抑制性中性粒细胞样单核细胞扩增 [scRNA-Seq]
• GSE314047 化疗诱导的反应性髓系造血导致小鼠和人类免疫抑制性中性粒细胞样单核细胞的扩增 [ATAC-seq]
• GSE304626 CXCL10/CXCR3轴对于维持三阴性乳腺癌的免疫休眠至关重要
• GSE291577 花粉发育中具有功能作用的siRNA的长距离运输[RNA-seq]
• 肺移植小鼠的GSE289883单细胞RNA测序
• GSE315740 有氧运动对慢性应激诱导的青少年小鼠抑郁样行为和海马转录组的影响
• GSE315736 PTP1B抑制通过增强SYK信号传导促进阿尔茨海默病模型中的小胶质细胞吞噬作用
• GSE315246 通过 FAP 靶向免疫疗法靶向动脉粥样硬化中受调控的血管平滑肌细胞 [Xenium 原位]
• GSE314854 通过FAP靶向免疫疗法治疗动脉粥样硬化中受调控的血管平滑肌细胞
• GSE314600 通过FAP靶向免疫疗法靶向动脉粥样硬化中受调控的血管平滑肌细胞 [SMC_Foam_Cell]
• GSE314598 通过 FAP 靶向免疫疗法靶向动脉粥样硬化中受调控的血管平滑肌细胞 [小鼠主动脉 BiTE]
• GSE314596 通过 FAP 靶向免疫疗法靶向动脉粥样硬化中受调控的血管平滑肌细胞 [Human_CITEseq]
• GSE314595 通过 FAP 靶向免疫疗法靶向动脉粥样硬化中受调控的血管平滑肌细胞 [Foam_Cell]
• GSE306867 PAR1激酶家族的两个成员PAR1b和PAR1a之间存在隐性冗余，这种冗余与PAR1b敲除小鼠的存活有关
• GSE200898 小鼠滑膜单细胞RNA测序
• GSE200283 小鼠滑膜批量 RNA 测序
• GSE318090 Functional architecture of cardiac TF regulatory landscapes in control of mammalian heart development
• GSE318002 单细胞分辨率下发育中鸡三叉神经节的细胞多样性
• GSE317933 苦参苷通过靶向DDX5/c-Myc轴抑制蛋白质合成来抑制急性髓系白血病
• GSE317744 胸腺 CD11c+ 细胞 [scRNA-seq]
• GSE289429 肿瘤抑制因子 STAG2 与 DREAM 协同作用，维持尿路上皮的静止状态 [Hi-C]
• GSE288712 肿瘤抑制因子STAG2与DREAM协同作用，维持尿路上皮细胞的静止状态。