详细内容（前10条）

1. ⭐ GSE315799 CGRP信号通路将肿瘤相关疼痛与口腔鳞状细胞癌的免疫逃逸联系起来

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、carcinoma、immune
• 📝 描述：Contributors : Amin Reza Nikpoor ; Maryam Ahmadi ; Sebastien Talbot ; Nicole ScheffSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPeripheral sensory nerves are thought to contribute to solid tumor growth, particularly in oral squamous cell carcinoma (OSCC); however, the link between pain and immunosuppression remains unresolved. Here, using a prospective observational study, we demonstrate an inverse relationship between OSCC-induced pain mediated by calcitonin gene-related peptide (CGRP)-expressing nerves and tumor-associated immunity. Bulk RNA sequencing of tumor-innervating sensory neurons from mice revealed differential regulation of genes associated with excitability, neurotransmission, and axonal sprouting. Using a gain-of-function approach with persistent stimulation of peptidergic afferents, we show that sensory neurons promote oral tongue tumor growth and limit the activation of effective anti-tumor immune responses via efferent CGRP release. Conversely, loss-of-function approaches—including local ablation of nociceptive nerves and systemic CGRP receptor antagonism—slowed tumor growth and improved anti-tumor immunity. Targeting CGRP may therefore represent a therapeutic strategy in OSCC to reduce pain and improve treatment response.
• 🔗 查看原文

2. ⭐ GSE291434 BMDM-T细胞共培养系统在有或无肿瘤抗原和IL-1β或IL-18存在下的批量RNA测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、antigen、RNAseq
• 📝 描述：Contributors : Ziqi Zhang ; Lei ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo investigate the transcriptome changes of BMDM-T cell co-culture system, spleen lyphocytes were cultured with WT or Tmem175-/- BMDMs in 1640 supplied with FBS, m-CSF, and IL-2 for 48 hours. Tumor antigens, IL-1beta (500 pg/ml), or IL-18 (500 pg/ml) were added during the co-culture period. At the end of the culture, cells were harvested for RNA seq.
• 🔗 查看原文

3. ⭐ GSE278149 多组学分析揭示鼻窦鳞状细胞癌的分子图谱和异质性肿瘤微环境

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、carcinoma、tumor microenvironment
• 📝 描述：Series Type : Methylation profiling by genome tiling array ; Genome binding/occupancy profiling by high throughput sequencing ; Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
• 🔗 查看原文

4. ⭐ GSE314637 RNA-seq分析巨噬细胞NFATc3或ASK1对代谢功能障碍相关脂肪性肝炎的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、metabolic、RNA-seq
• 📝 描述：Contributor : Si-Jia LiangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo explore the roles of macrophage NFATc3 and ASK1 in metabolic dysfunction-associated steatohepatitis, we generated macrophage-specific Nfatc3 knockout mice (Nfatc3-MKO), macrophage-specific Ask1 knockout mice (Ask1-MKO),macrophage-specific double knockout (MDKO) mice, macrophage-specific Nfatc3 knockin mice (Nfatc3-MKI), and macrophage-specific Ask1 knockin mice (Ask1-MKI). Lyz2-Cre mice were used as control. Eight-week-old male Lyz2-Cre, Nfatc3-MKO, Ask1-MKO, and MDKO mice were treated with methionine- and choline-deficient (MCD) diet for 4 weeks. Subsequently, mouse liver tissues were isolated and subjected to RNA sequencing analysis. In addition, mouse bone marrow-derived macrophages (BMDMs) were isolated from Lyz2-Cre, Nfatc3-MKI, and Ask1-MKI mice and were also subjected to RNA sequencing analysis.
• 🔗 查看原文

5. GSE311919 代谢重编程调控斑马鱼尾鳍再生过程中的组蛋白乳酸化

• ✍️ 作者：未知作者
• 🏷️ 关键词：metabolic、histone
• 📝 描述：Contributors : Jorge Borbinha ; Raquel Lourenço ; Ana S Brandão ; Daniel Ribeiro ; Ana Carvalho ; Rune Matthiesen ; António JacintoSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioTissue regeneration relies on precise molecular mechanisms controlling cell fate transitions, with cell metabolism emerging as a key regulator. Lactate-derived histone lactylation has recently been identified as an important epigenetic modification involved in the regulation of gene expression across various biological processes. In this study, we report an increase in global histone lactylation and H3K18Lac levels in the mesenchyme and osteoblasts of the zebrafish caudal fin, during the early stages of regeneration. Our findings demonstrate that this epigenetic modification is functionally regulated by increased lactate levels, while inhibition of glycolysis and lactate production significantly reduces histone lactylation. These results suggest that metabolic reprogramming, in response to caudal fin injury, regulates histone lactylation, potentially modulating gene expression essential for cell plasticity and proliferation. This study expands our understanding of how metabolic-epigenetic interactions influence regenerative processes, providing valuable insights for the development of novel therapeutic strategies to enhance tissue repair.
• 🔗 查看原文

6. GSE311253 RNA-seq 分析 ADAM10 抑制在 DL​​D-1 和 SW620 结直肠癌细胞系中的表达情况

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、RNA-seq
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis study examines the transcriptomic effects of ADAM10 inhibition in two human colorectal cancer cell lines, DLD-1 and SW620. Cells were treated with vehicle control or the conformation-specific ADAM10 monoclonal antibody 1H5 for 48 hours, followed by extraction of total RNA and paired-end RNA sequencing. The dataset enables comparative analysis of ADAM10-regulated pathways across different CRC genetic backgrounds, including alterations in Notch and EGFR signaling, metabolic gene programs, and other pathways associated with tumor progression.
• 🔗 查看原文

7. GSE310170 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (RNA-seq)

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、RNA-seq
• 📝 描述：Contributors : Janith A Seneviratne ; Melanie A Eckersley-MaslinSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensEmbryonic regulators are often re-expressed in cancers, however the functional and molecular significance of this is not always understood. The epigenetic priming factors Developmental Pluripotency Associated 2 and 4 (DPPA2/4) have crucial roles in early development and are implicated in cancer pathogenesis. We reveal in non-small cell lung cancer (NSCLC), DPPA2/4 co-expression is associated with poorly differentiated tumours and impaired patient outcomes. Biochemically, human DPPA2/4 multimerise for their protein stability and enhanced nucleosome binding activity. In NSCLC cells, DPPA2/4 bind CG-rich sequences including promoters of developmental, Wnt signaling and catabolic genes. Chromatin state modelling revealed DPPA2/4 preferentially bind active H3K4me3 and H3K27ac domains that were intriguingly also enriched for PRC1 and its product H2AK119ub, validated by H3K4me3-H2AK119ub sequential ChIP. Knockdown experiments revealed DPPA2/4 were required to maintain RING1B and H2AK119ub at these domains. Surprisingly, despite the presence of PRC2.1, these regions lacked any detectable H3K27me3, suggesting an uncoupling between the recruitment of PRC2 to chromatin and its catalytic product. When exogenously over-expressed in NSCLC cells where they are not normally present, DPPA2/4 bind to and promote active chromatin states, resulting in an increase in vivo xenograft tumour growth. Our results demonstrate how in NSCLC cells, DPPA2/4 act as molecular amplifiers of active and poised chromatin. Together, this highlights how aberrant re-activation of embryonic factors in cancers may take on new functions, promoting tumourigenesis.
• 🔗 查看原文

8. GSE310167 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (ChIP-seq)

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、ChIP-seq
• 📝 描述：Contributors : Janith A Seneviratne ; Melanie A Eckersley-MaslinSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensEmbryonic regulators are often re-expressed in cancers, however the functional and molecular significance of this is not always understood. The epigenetic priming factors Developmental Pluripotency Associated 2 and 4 (DPPA2/4) have crucial roles in early development and are implicated in cancer pathogenesis. We reveal in non-small cell lung cancer (NSCLC), DPPA2/4 co-expression is associated with poorly differentiated tumours and impaired patient outcomes. Biochemically, human DPPA2/4 multimerise for their protein stability and enhanced nucleosome binding activity. In NSCLC cells, DPPA2/4 bind CG-rich sequences including promoters of developmental, Wnt signaling and catabolic genes. Chromatin state modelling revealed DPPA2/4 preferentially bind active H3K4me3 and H3K27ac domains that were intriguingly also enriched for PRC1 and its product H2AK119ub, validated by H3K4me3-H2AK119ub sequential ChIP. Knockdown experiments revealed DPPA2/4 were required to maintain RING1B and H2AK119ub at these domains. Surprisingly, despite the presence of PRC2.1, these regions lacked any detectable H3K27me3, suggesting an uncoupling between the recruitment of PRC2 to chromatin and its catalytic product. When exogenously over-expressed in NSCLC cells where they are not normally present, DPPA2/4 bind to and promote active chromatin states, resulting in an increase in vivo xenograft tumour growth. Our results demonstrate how in NSCLC cells, DPPA2/4 act as molecular amplifiers of active and poised chromatin. Together, this highlights how aberrant re-activation of embryonic factors in cancers may take on new functions, promoting tumourigenesis.
• 🔗 查看原文

9. GSE310144 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (ATAC-seq)

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、ATAC-seq
• 📝 描述：Contributors : Janith A Seneviratne ; Melanie A Eckersley-MaslinSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensEmbryonic regulators are often re-expressed in cancers, however the functional and molecular significance of this is not always understood. The epigenetic priming factors Developmental Pluripotency Associated 2 and 4 (DPPA2/4) have crucial roles in early development and are implicated in cancer pathogenesis. We reveal in non-small cell lung cancer (NSCLC), DPPA2/4 co-expression is associated with poorly differentiated tumours and impaired patient outcomes. Biochemically, human DPPA2/4 multimerise for their protein stability and enhanced nucleosome binding activity. In NSCLC cells, DPPA2/4 bind CG-rich sequences including promoters of developmental, Wnt signaling and catabolic genes. Chromatin state modelling revealed DPPA2/4 preferentially bind active H3K4me3 and H3K27ac domains that were intriguingly also enriched for PRC1 and its product H2AK119ub, validated by H3K4me3-H2AK119ub sequential ChIP. Knockdown experiments revealed DPPA2/4 were required to maintain RING1B and H2AK119ub at these domains. Surprisingly, despite the presence of PRC2.1, these regions lacked any detectable H3K27me3, suggesting an uncoupling between the recruitment of PRC2 to chromatin and its catalytic product. When exogenously over-expressed in NSCLC cells where they are not normally present, DPPA2/4 bind to and promote active chromatin states, resulting in an increase in vivo xenograft tumour growth. Our results demonstrate how in NSCLC cells, DPPA2/4 act as molecular amplifiers of active and poised chromatin. Together, this highlights how aberrant re-activation of embryonic factors in cancers may take on new functions, promoting tumourigenesis.
• 🔗 查看原文

10. GSE272003 TAS1440，一种 LSD1 抑制剂，通过转录重编程破坏 INSM1-LSD1 复合物，从而激活神经内分泌小细胞肺癌中的肿瘤抑制通路 [ChIP-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、ChIP-seq
• 📝 描述：Contributors : Takumitsu Machida ; Yingbo Gong ; Sayaka Tsukioka ; Atsushi Onodera ; Akitoshi Nakayama ; Naoko Hashimoto ; Takahiro Fuchigami ; Satoshi Yamashita ; Tatsuya Suzuki ; Ryo Hatanaka ; Yasuo Kodama ; Shuichi Ohkubo ; Tomoaki TanakaSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensSmall cell lung cancer (SCLC) is characterized by aggressive progression and limited treatment options, necessitating novel therapeutic strategies. Lysine-specific histone demethylase 1A (LSD1), a key epigenetic enzyme essential for maintaining the neuroendocrine phenotype, represses NOTCH and TGF-β signaling. Reactivation of these pathways suppresses proliferation and induces differentiation, underscoring LSD1’s potential as a therapeutic target. However, the molecular mechanisms underlying LSD1 inhibition and chemoresistance drivers remain poorly understood. Here, through structure-based engineering, we developed TAS1440, a potent histone H3-competitive LSD1 inhibitor designed to enhance specificity and reduce off-target effects. Unlike covalent inhibitors, which bind irreversibly to the FAD cofactor in LSD1, TAS1440 non-covalently targets the histone H3-binding pocket, improving safety and efficacy. TAS1440 demonstrated superior anti-proliferative activity in SCLC-A cells with high INSM1 and ASCL1 expression. In xenograft models, TAS1440 achieved over 70% tumor growth inhibition with minimal toxicity, highlighting its potential for SCLC. Mechanistically, integrated studies revealed that TAS1440 exerts dual mechanisms: direct inhibition of LSD1 enzymatic activity and disruption of LSD1-repressive complexes, thereby altering the histone modification landscape and activating transcription factors such as INSM1 and SMAD2. These actions coordinately reprogram tumor-suppressive pathways, including TGF-β and NOTCH signaling, positioning TAS1440 as an effective epigenetic therapy for SCLC. Importantly, Loss of LSD1 enzymatic activity and INSM1 knockout abrogated TAS1440’s effects, elucidating its mechanism of action and uncovering potential resistance pathways. These findings establish TAS1440 as a next-generation LSD1 inhibitor with dual actions on transcriptional regulation and epigenetic reprogramming. TAS1440 holds significant promise as a targeted therapy for SCLC, particularly for the SCLC-A subtype with high INSM1 expression.
• 🔗 查看原文

💡 该来源还有 23 条内容，详见 文末

详细内容（全部1条）

1. 在 Posit Connect Cloud 上使用 freeCount 进行生物信息学分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：bioinformatics
• 📝 描述：The easiest way to use the freeCount R Shiny applications online is through Posit Connect Cloud, which is an online platform that simplifies the deployment of data applications and documents. Continue reading: Bioinformatics Analysis on Posit Connect Cloud with freeCount
• 🔗 查看原文

详细内容（全部6条）

1. ⭐ Immunai公司扩大学术合作，利用单细胞RNA测序技术绘制免疫反应图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、sequencing、single-cell
• 📝 描述：Immunai partners with leading academic centers to provide single-cell RNA sequencing and multi-omic data, building high-resolution immune maps across autoimmunity, cancer immunotherapy, and cell therapy…
• 🔗 查看原文

2. DBiTplus——在同一组织切片上整合基于成像和基于测序的空间组学图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、spatial
• 📝 描述：DBiTplus combines RNA sequencing and protein imaging on the same tissue section, enabling single-cell spatial maps that reveal how genes and proteins interact in health and disease…
• 🔗 查看原文

3. TSniffer——对RNA测序数据中的RNA编辑位点进行无偏倚的从头识别并量化编辑活性

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq
• 📝 描述：TSniffer enables unbiased detection and quantification of RNA editing from RNA sequencing data, helping researchers study ADAR-driven transcriptome changes across…
• 🔗 查看原文

4. 癌症如何扰乱大脑并引发焦虑和失眠

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have discovered that breast cancer can quietly throw the brain’s internal clock off balance—almost immediately after cancer begins. In mice, tumors flattened the natural daily rhythm of stress hormones, disrupting the brain-body feedback loop that regulates stress, sleep, and immunity. Remarkably, when researchers restored the correct day-night rhythm in specific brain neurons, stress hormone cycles snapped back into place, immune cells flooded the tumors, and the cancers shrank—without using any anti-cancer drugs.
• 🔗 查看原文

5. 科学家攻克癌症细胞疗法发展中的一个重大障碍

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Researchers have found a reliable way to grow helper T cells from stem cells, solving a major challenge in immune-based cancer therapy. Helper T cells act as the immune system’s coordinators, helping other immune cells fight longer and harder. The team discovered how to precisely control a key signal that determines which type of T cell forms. This advance could lead to ready-made cell therapies that are cheaper, faster, and easier to access.
• 🔗 查看原文

6. 一种常用止痛药可能正在悄然改变癌症风险。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Ibuprofen may be doing more than easing aches and pains—it could also help reduce the risk of some cancers. Studies have linked regular use to lower rates of endometrial and bowel cancer, likely because the drug dampens inflammation that fuels tumor growth. Researchers have even found it can interfere with genes cancer cells rely on to survive. Still, experts warn that long-term use carries risks and shouldn’t replace proven prevention strategies.
• 🔗 查看原文

• GSE272002 TAS1440，一种 LSD1 抑制剂，通过转录重编程破坏 INSM1-LSD1 复合物，从而激活神经内分泌小细胞肺癌中的肿瘤抑制通路 [RNA-seq]
• GSE223320 IL11 在侵袭性肝细胞癌中组织具有免疫逃逸能力的巨梁结构
• GSE316499 研究发现，普萘洛尔通过上调 CDK11 并诱导细胞周期阻滞，克服黑色素瘤对维莫非尼的耐药性。
• GSE314097 党参多糖通过巨噬细胞黏附和钙信号传导减轻布鲁氏菌外膜蛋白19诱导的子宫损伤
• GSE291346 单细胞 RNA 测序分析 Tmem175 KO 和 WT 小鼠肺转移微环境。
• GSE273303 使用 α-HER1 抗体进行 HER1 定位的 Cut&Tag-seq 分析，以及使用 α-H3K9me2 抗体进行 WT 和 her1 的 Cut&Tag-seq 分析
• GSE273302 野生型和 her1 水稻 RNA 测序数据
• GSE316731 内皮细胞特异性NOX1激活通过骨骼肌线粒体功能障碍驱动肥胖和代谢综合征，并具有新的遗传特征
• GSE316686 用agrin或MuSK抗体处理的C2C12肌管的总RNA测序
• GSE316557 乳酸介导的胆固醇摄取通过 SCARB1-自噬轴促进肝癌进展
• GSE316433 研究发现，围手术期应用岩藻聚糖会诱发免疫抑制变化，导致术后神经认知功能恢复未见获益。
• GSE316327 HP1α 缺失和 TGFβ 激活对 3D 基因组组织具有拮抗作用
• GSE316315 溶瘤腺病毒治疗后原位胶质母细胞瘤的单细胞RNA测序
• GSE314638 小鼠骨髓来源巨噬细胞中 NFATc3 过表达引起的染色质状态变化的全基因组图谱
• GSE310173 胚胎干细胞因子DPPA2/4扩增非小细胞肺癌中活性H3K4me3-H2AK119ub染色质结构域
• GSE310171 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (reChIP-seq)
• GSE310165 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (ChIPRx-seq)
• GSE310161 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (CUT&Run)
• GSE310160 胚胎干细胞因子 DPPA2/4 扩增非小细胞肺癌中的活性 H3K4me3-H2AK119ub 染色质结构域 (BS-seq)
• GSE302851 多发性骨髓瘤中的甲基化变异性和LINE-1激活
• GSE285078 MRTFA-SRF驱动的基因表达通过控制癌细胞中细胞质Ca2+和K+水平来促进细胞刚度 II
• GSE241211 少突胶质细胞前体细胞通过BMP4驱动获得阿尔茨海默病相关状态，从而调节小胶质细胞向神经保护表型的转变
• GSE223356 TFRC-RNA 相互作用组揭示了人类肾小管系膜细胞中与 IgAN 相关的基因的表达和选择性剪接调控 [RNA-seq]