详细内容（前10条）

1. ⭐ GSE297627 乳腺癌中乳腺肿瘤起始细胞群和免疫逃逸途径的衰老相关差异 [scRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immune、aging、scRNA
• 📝 描述：Contributors : Pengze Yan ; Kornelia PolyakSeries Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusAging is a major risk factor for breast cancer, yet how it shapes tumor development, molecular phenotype, and immune evasion remains incompletely understood. Deciphering how aging influences cancer development is critical for improving risk assessment, prevention, and treatment. Here, using a N-nitroso-N-methylurea (NMU)-induced rat mammary tumor model that recapitulates key features of human breast cancer, we integrated bulk and single-cell transcriptomics, whole-exome sequencing, and histopathological analysis to dissect age-associated differences in tumorigenesis. We found that age at NMU exposure critically influences tumor incidence, mutational burden, subtype, and immune microenvironment. Tumors arising in aged rats originate from luminal progenitor-like cells with increased genomic instability, suppressed immune infiltration, and impaired antigen presentation linked to loss of heterozygosity at Chr 20p. These age-associated epithelial and immune changes were conserved in human breast cancers, where loss of the homologous Chr 6p region correlated with reduced lymphocyte infiltration and shorter relapse-free survival. These findings reveal that aging alters the tumor-initiating cell population and promotes immune-evasive tumor states through chromosomal instability-driven antigen presentation defects. Our work provides mechanistic insight into the reduced efficacy of immunotherapy in older patients.
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2. ⭐ GSE297628 乳腺癌中乳腺肿瘤起始细胞群和免疫逃逸途径的衰老相关差异

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immune、aging
• 📝 描述：Series Type : Other ; Expression profiling by high throughput sequencingOrganism : Rattus norvegicusThis SuperSeries is composed of the SubSeries listed below.
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3. ⭐ GSE297548 乳腺癌中乳腺肿瘤起始细胞群和免疫逃逸途径的衰老相关差异 [bulk_RNA_seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immune、aging
• 📝 描述：Contributors : Pengze Yan ; Kornelia PolyakSeries Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusAging is a major risk factor for breast cancer, yet how it shapes tumor development, molecular phenotype, and immune evasion remains incompletely understood. Deciphering how aging influences cancer development is critical for improving risk assessment, prevention, and treatment. Here, using a N-nitroso-N-methylurea (NMU)-induced rat mammary tumor model that recapitulates key features of human breast cancer, we integrated bulk and single-cell transcriptomics, whole-exome sequencing, and histopathological analysis to dissect age-associated differences in tumorigenesis. We found that age at NMU exposure critically influences tumor incidence, mutational burden, subtype, and immune microenvironment. Tumors arising in aged rats originate from luminal progenitor-like cells with increased genomic instability, suppressed immune infiltration, and impaired antigen presentation linked to loss of heterozygosity at Chr 20p. These age-associated epithelial and immune changes were conserved in human breast cancers, where loss of the homologous Chr 6p region correlated with reduced lymphocyte infiltration and shorter relapse-free survival. These findings reveal that aging alters the tumor-initiating cell population and promotes immune-evasive tumor states through chromosomal instability-driven antigen presentation defects. Our work provides mechanistic insight into the reduced efficacy of immunotherapy in older patients.
• 🔗 查看原文

4. ⭐ GSE297544 乳腺癌中乳腺肿瘤起始细胞群和免疫逃逸途径的衰老相关差异 [外显子组测序]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immune、aging
• 📝 描述：Contributors : Pengze Yan ; Kornelia PolyakSeries Type : OtherOrganism : Rattus norvegicusAging is a major risk factor for breast cancer, yet how it shapes tumor development, molecular phenotype, and immune evasion remains incompletely understood. Deciphering how aging influences cancer development is critical for improving risk assessment, prevention, and treatment. Here, using a N-nitroso-N-methylurea (NMU)-induced rat mammary tumor model that recapitulates key features of human breast cancer, we integrated bulk and single-cell transcriptomics, whole-exome sequencing, and histopathological analysis to dissect age-associated differences in tumorigenesis. We found that age at NMU exposure critically influences tumor incidence, mutational burden, subtype, and immune microenvironment. Tumors arising in aged rats originate from luminal progenitor-like cells with increased genomic instability, suppressed immune infiltration, and impaired antigen presentation linked to loss of heterozygosity at Chr 20p. These age-associated epithelial and immune changes were conserved in human breast cancers, where loss of the homologous Chr 6p region correlated with reduced lymphocyte infiltration and shorter relapse-free survival. These findings reveal that aging alters the tumor-initiating cell population and promotes immune-evasive tumor states through chromosomal instability-driven antigen presentation defects. Our work provides mechanistic insight into the reduced efficacy of immunotherapy in older patients.
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5. ⭐ GSE301618 RBP4 通过抑制 PI3K/AKT 信号通路和促进巨噬细胞 M1 型极化来抑制舌鳞状细胞癌的进展

• ✍️ 作者：未知作者
• 🏷️ 关键词：carcinoma、macrophage、pathway
• 📝 描述：Contributors : Ying Yan ; Nan Miao ; Xiaofeng WangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTongue squamous cell carcinoma (TSCC) is a common type of oral mucosal epithelial malignancy that can severely affect patient quality of life. Therefore, novel therapeutic strategies for TSCC are needed. This study aimed to investigate the role and mechanism of action of retinol-binding protein 4 (RBP4) in TSCC progression and its effect on tumor-associated macrophages. To this end, TSCC cell lines with RBP4 over-expression and knockdown were constructed, and effects of RBP4 expression on the proliferation and inva-sive abilities of TSCC cells were verified using ex vivo experiments. Furthermore, a tumor cell-macrophage co-culture model was established to assess the effect of RBP4 on macrophage polarization. RBP4 overex-pression reduced the phosphorylation of the PI3K/Akt/mTOR pathway and inhibited tumor cell proliferation by regulating Snail levels; RBP4 inhibited TSCC cells from undergoing epithelial-mesenchymal transition. In addition, RBP4 promoted the activation of NF-κB signaling pathway, leading to macrophage polarization toward M1 type and inhibiting TSCC growth. We found for the first time that RBP4 affects the prolifera-tion and migration of TSCC cells by inhibiting the activation of the PI3K-Akt signaling pathway, and that RBP4 promotes the M1-type polarization of tumor-associated macrophages, which contributes to their on-cogenic effects.
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6. ⭐ GSE284698 在激素受体阳性乳腺癌大鼠模型中评估靶向和免疫联合疗法[scRNA]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune、scRNA
• 📝 描述：Contributors : Ernesto Rojas Jimenez ; Triet Bui ; Pengze Yan ; Marco Seehawer ; Jun Nishida ; Kornelia PolyakSeries Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusBreast cancer hormone receptor (HR) estrogen and progesterone (ER/PR) positive tumors were generated in an outbred rat model (Sprague-Dawley) through the injection of the carcinogen N-nitrosomethylurea (NMU). These tumors were treated with immune checkpoint blockade (anti PD-L1) in combination with targeted therapies, including an TBK1/IKBKE inhibitor, a KMT5B/C inhibitor, a TGF-b inhibitor, and a selective estrogen receptor degrader (SERD). This dataset includes single-cell RNA sequencing (scRNA-seq) data from tumors subjected to various treatment setups. Whole-tumor digestion was performed to preserve a comprehensive representation of the tumor microenvironment. The study aims to identify mechanisms that sensitize HR postive tumor subtypes to immune checkpoint blockade therapy.
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7. ⭐ GSE315939 可扩展的单细胞总RNA测序统一了编码和非编码转录组学

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、single-cell、transcriptomics
• 📝 描述：Contributor : Alina IsakovaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCurrent single-cell RNA atlases largely capture polyadenylated transcripts while missing critical regulatory layers from non-coding RNA. To address this, we developed TotalX - a generalizable framework that adapts Smart-seq total RNA profiling for use in droplet-based platforms, and captures a broad complement of coding and non-coding RNAsusing a unified pipeline. Applying this approach to developing human brain, we generate a dataset mapping diverse RNA biotypes across all neuronal and non-neuronal lineages, revealingbiotype-specific expression programs with cell-type and temporal specificity. Tracking miRNA dynamics in Cajal–Retzius neurons, transient and early-born neurons in the cortex, we show the enrichment and target anti-correlation of MIR137, associated with schizophrenia and intellectual disability, suggesting tight regulatory control. We apply TotalX to human peripheral blood mononuclear cells and identify transcriptional modules combining coding and non-coding RNAs and tRNA dynamics. Additionally, we analyze dengue-infected hepatocytes and capture non-adenylated viral transcripts that distinguish infection states. This expanded coverage helps with understanding cellular identity and gene regulation at atlas scale
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8. GSE215220 RNA-Seq 检测了经铜螯合剂处理的 VUMC-DIPG10 和 HSJD-DIPG007 人弥漫性内生性脑桥胶质瘤细胞

• ✍️ 作者：未知作者
• 🏷️ 关键词：glioma、RNA-seq
• 📝 描述：Contributors : Federica Saletta ; Daniele Mercatelli ; Federico Giorgi ; Orazio VittorioSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe report the application of RNA-sequencing of VUMC-DIPG10 and HSJD-DIPG007 human diffuse intrinsic pontine glioma cells treated with copper chelator Tetraethylenepentamine (TEPA) for 24 hours. We show transcriptome changes following copper chelation vs. control untreated cells.vs. control samples
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9. GSE315761 RNA-seq 分析人肠上皮 FHC 细胞中 METTL1 敲低的影响

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、regex:intestin(e|al)
• 📝 描述：Contributors : Lichao Yang ; Lianwen YuanSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMETTL1 is a key methyltransferase responsible for N7-methylguanosine (m7G) modification of RNA and has been implicated in multiple cellular processes. However, its role in human intestinal epithelial cells remains incompletely understood. In this study, we performed RNA sequencing (RNA-seq) to characterize transcriptomic changes induced by METTL1 knockdown in human fetal intestinal epithelial (FHC) cells. Stable METTL1 knockdown was achieved using lentiviral short hairpin RNA (shRNA), while control cells were infected with a non-targeting control shRNA lentivirus (sh-Ctrl). Knockdown efficiency was validated prior to sequencing. Total RNA was extracted from METTL1-knockdown and sh-Ctrl FHC cells, with three independent biological replicates per group. This dataset provides a transcriptomic resource for investigating the regulatory role of METTL1 in intestinal epithelial cells.
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10. GSE302754 葡萄籽提取物干预小鼠皮肤老化实验研究中NSPCC1干预的小鼠皮肤转录组分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、transcriptome
• 📝 描述：Contributors : Zhen Zhang ; Zhaoyun Xue ; Yufang Wang ; Yihan Zhang ; Jianwen Chen ; Ruikun HeSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo investigate the effects of Grape Seed Extract NSPCC1 on D-galactose combined with UVA/B-induced skin photoaging model and related transcription factors in C57BL/6J mice.
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1. ⭐ 注射疗法可将沉睡的肿瘤免疫细胞转化为抗癌战士。

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immune
• 📝 描述：KAIST researchers have developed a way to reprogram immune cells already inside tumors into cancer-killing machines. A drug injected directly into the tumor is absorbed by macrophages, prompting them to recognize and attack cancer cells while activating nearby immune defenses. This eliminates the need for lab-based cell extraction and modification. In animal models, the strategy significantly slowed tumor growth and sparked strong anticancer immune responses.
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• GSE286115 尿苷代谢作为化疗耐药性B细胞急性淋巴细胞白血病（B-ALL）的可靶向代谢“阿喀琉斯之踵”。
• GSE286099 LncRNA 3222401L13Rik 在衰老星形胶质细胞中上调，并通过与 Npas3 相互作用调节神经元支持功能 [年轻]
• GSE315655 缺氧可恢复酸中毒引起的癌细胞扩散抑制
• 利用GSE173701下一代测序技术评估miR26b对动脉粥样硬化小鼠血管的影响
• GSE316028 丙酮酸羧化酶通过激活AKT通路促进糖酵解和子宫内膜异位症的进展
• GSE301570 利用细胞积累技术研究Meflin过表达对三维培养中成纤维细胞转录组的影响
• GSE315933 研究发现，石蒜碱可通过 TKT/ATF3 通路减少胆汁酸的积累，从而缓解肝内胆汁淤积。
• GSE315886 AC16细胞中CTCF ChIP-seq：保守的染色质结构程序是损伤诱导的心肌细胞增殖的基础
• GSE315789 α特异性PI3K抑制剂通过抑制肝星状细胞活化来提高肝癌化疗疗效
• GSE314950 小鼠蓝斑中的致病性tau蛋白导致去甲肾上腺素能过度活跃和类似于阿尔茨海默病前驱期的神经精神表型
• GSE312171 粪便微生物移植可减轻慢性结肠炎小鼠的心脏重塑和功能障碍
• GSE312149 肿瘤细胞中的 circRNA 干扰谱分析。
• GSE312147 雌激素治疗的肿瘤细胞基因表达谱分析
• GSE298078 铜绿假单胞菌中全局翻译激活因子的发现 [RNA-seq]
• GSE289618 LncRNA WEE1-AS 通过激活线粒体 Cdk1/Cyclin B1 来协调氧化脂肪酸代谢
• GSE286514 柠檬酸盐的胞质运输可保护胰腺癌细胞免受营养胁迫下的铁死亡。
• GSE284159 人类可扩增胰腺祖细胞-胰岛系统的功能基因调控网络及其广泛应用 [RNA-Seq]
• GSE284101 人类可扩增胰腺祖细胞-胰岛系统的功能基因调控网络及其广泛应用 [scRNA-Seq]
• GSE283761 甘薯受伤后 IbmiR168-3p-IbKTN80 模块在茉莉酸生物合成途径中的系统性作用
• GSE233078 单细胞分辨率药物对 ZSF1 大鼠糖尿病肾病中肾素-血管紧张素-醛固酮阻滞的影响