详细内容（前10条）

1. ⭐ GSE281519 PARP抑制剂的溶酶体隔离驱动卵巢癌中的异质性积累以提高疗效[空间转录组学]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Carmen Ramirez Moncayo ; Restuadi Restuadi ; Louise FetsSeries Type : OtherOrganism : Homo sapiensPARP inhibitors have had a transformative impact in the treatment of high-grade serous ovarian cancer (HGSOC), but resistance remains a clinical hurdle. Increased expression of ABC drug efflux transporters has been identified as a resistance mechanism in patients but whether intracellular concentrations of these drugs is heterogeneous within tumours remains unclear. We developed a patient-derived explant multi-modal imaging pipeline which demonstrated significant cell-intrinsic heterogeneity of PARP inhibitor accumulation, both between patients and within tumours. Spatial transcriptomics revealed enrichment of apoptotic signatures in high-drug compared to low-drug regions, as well as metabolic and ECM-related differences. While some cases demonstrated a clear anticorrelation of PARP inhibitor levels with MDR1(ABCB1), this was not universal. Rucaparib, an intrinsically fluorescent PARP inhibitor accumulates heterogeneously at the single cell-level in established cell lines, and omics approaches revealed that this is driven by lysosomal sequestration, a pattern also observed in patient samples. Perturbation of lysosomal content altered intracellular levels of rucaparib and niraparib and impacted efficacy suggesting that lysosomes act as a drug reservoir to improve drug response.
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2. ⭐ GSE315178 9-PAHSA 调控 TCR-T 细胞命运以增强抗肿瘤免疫力 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、T cell、ATAC-seq
• 📝 描述：Contributor : Xiaozhen ZhangSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusWe identify the endogenous lipid 9-PAHSA as a potent enhancer of T cell anti-tumor function and stemness, validated in murine models and a clinical trial. Mechanistically, we identify ICAM2 as a direct surface sensor for 9-PAHSA and delineate a downstream signaling axis whereby the ICAM2-Ezrin-mTORC2 cascade drives FOXO1 O-GlcNAcylation at S318, facilitating its nuclear translocation to enact a stem-like transcriptional program. Translating this discovery, we engineered armored human TCR-T cells with potentiated ICAM2 expression, which achieve superior tumor control through their enhanced intrinsic efficacy and a remarkable ability to ignite a coordinated and durable host anti-tumor immune response. Our work establishes a new paradigm of metabolite-sensing in T cell biology and provides a compelling therapeutic strategy to potentiate cellular immunotherapy for intractable cancers.
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3. ⭐ GSE310383 年龄无关且可靶向的转录因子网络调控衰老人类 CD8+ T 细胞衰老 [ATAC-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、T cell、ATAC-seq
• 📝 描述：Contributors : Paolo S Turano ; Elizabeth Akbulut ; Hannah K Dewald ; Themistoklis Vasilopoulos ; Patricia Fitzgerald-Bocarsly ; Utz Herbig ; Ricardo Iván Martínez-ZamudioSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensAging impairs immunity in part through the accumulation of CD8+ T cells with senescence features, including a pro-inflammatory transcriptome and impaired proliferative capacity. Using senescent cell isolation coupled with multiomic profiling, we defined the epigenetic regulation of CD8+ T cell senescence in healthy younger and older humans. We discovered that the transition to senescence was controlled by chromatin state-specific transcription factor (TF) networks whose composition was largely insensitive to donor age. These TF networks mediated widespread enhancer remodeling, causing repression of genes essential for proliferation as well as upregulation of inflammatory and secretory pathways. Inhibition of AP1, KLF5, or RUNX2 modulated the transcriptional output and partially restored the blunted transcriptional response to stimulation of senescent CD8+ T cells. Our data also revealed CD8+ T cell senescence gene signatures predictive of response to CAR-T cell therapy in diffuse large B cell lymphomas. Overall, our study defines the gene regulatory mechanisms of senescence in human CD8+ T cells, provides a resource for the interrogation of CD8+ T cell senescence in disease, highlights TF network perturbation as a viable strategy to manipulate the senescence state of CD8+ T cells, and reveals senescent CD8+ T cell gene signatures as valuable prognostic tools for immunotherapy outcome.
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4. ⭐ GSE310382 年龄无关且可靶向的转录因子网络调控衰老人类 CD8+ T 细胞衰老 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、T cell、RNA-seq
• 📝 描述：Contributors : Paolo S Turano ; Elizabeth Akbulut ; Hannah K Dewald ; Themistoklis Vasilopoulos ; Patricia Fitzgerald-Bocarsly ; Utz Herbig ; Ricardo Iván Martínez-ZamudioSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAging impairs immunity in part through the accumulation of CD8+ T cells with senescence features, including a pro-inflammatory transcriptome and impaired proliferative capacity. Using senescent cell isolation coupled with multiomic profiling, we defined the epigenetic regulation of CD8+ T cell senescence in healthy younger and older humans. We discovered that the transition to senescence was controlled by chromatin state-specific transcription factor (TF) networks whose composition was largely insensitive to donor age. These TF networks mediated widespread enhancer remodeling, causing repression of genes essential for proliferation as well as upregulation of inflammatory and secretory pathways. Inhibition of AP1, KLF5, or RUNX2 modulated the transcriptional output and partially restored the blunted transcriptional response to stimulation of senescent CD8+ T cells. Our data also revealed CD8+ T cell senescence gene signatures predictive of response to CAR-T cell therapy in diffuse large B cell lymphomas. Overall, our study defines the gene regulatory mechanisms of senescence in human CD8+ T cells, provides a resource for the interrogation of CD8+ T cell senescence in disease, highlights TF network perturbation as a viable strategy to manipulate the senescence state of CD8+ T cells, and reveals senescent CD8+ T cell gene signatures as valuable prognostic tools for immunotherapy outcome.
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5. ⭐ GSE218539 人类左心耳和肺静脉袖的空间转录组学

• ✍️ 作者：未知作者
• 🏷️ 关键词：spatial、spatial transcriptomics、transcriptomics
• 📝 描述：Contributors : Han Sun ; Juying Han ; Christine S Moravec ; Toshihiro Okamoto ; Kenneth R McCurry ; A M Gillinov ; Mina K Chung ; Davd R Van Wagoner ; John Barnard ; Jonathan D SmithSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensHuman left atrial appendage (LAA) and pulmonary vein (PV) tissues were obtained from unused lung or heart transplant donors. Paired LAA and PV sleeve tissues were obtained from two subjects, and an additional 6 PV samples were obtained from other donors. After tissue sectioning and staining, spatial RNAseq was performed. mRNAs from~1000 to 2500 genes were sequenced in each spatial area. Seurat clustering yielded 15 different clusters. These grossly split into two segregating populations, the left and right sides, with one connecting population. Multiple cells and cell types may reside in each 55 µm diameter spatial area. A clear asymmetry of clusters was observed in the PV sections, with less asymmetry in the LAA sections. Cell-type marker genes derived from human LAA single nuclei RNAseq were used to determine the dominant cell types in the 15 different clusters. The left side segregating clusters were enriched in cardiomyocytes, while the right side segregating clusters were enriched for other cell types including fibroblasts, vascular smooth muscle cells, endothelial cells, and adipocytes. Spatial transcriptomics clearly resolved the venous, cardiomyocyte, and epicardial regions of the PV tissues, as well as fibrotic regions in LAAs and PVs. Spatial expression of the AF-associated genes PITX2, SHOX2, and HCN4 was also mapped, confirming higher expression of the cardiac master transcription factor SHOX2 in the PV vs. LAA tissues. Expression of the hyperpolarization-activated ion channel HCN4 was sporadic in both PV and LAA specimens.
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6. ⭐ GSE284622 抗原选择的肠道 IgA 由 IgG1 生发中心 B 细胞产生

• ✍️ 作者：未知作者
• 🏷️ 关键词：antigen、gut、regex:gut(-?microbiome)?
• 📝 描述：Series Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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7. ⭐ GSE284621 抗原选择的肠道 IgA 由 IgG1 生发中心 B 细胞 (BCR) 产生

• ✍️ 作者：未知作者
• 🏷️ 关键词：antigen、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Emily Siniscalco ; Hailong Meng ; Gisela Gabernet ; Stephanie Eisenbarth ; Steven KleinsteinSeries Type : OtherOrganism : Mus musculusDetermine whether IgA B cells are derived from IgE B cells during allergy; understand whether IgE and IgA B cells induced during allergy share clonal relationships in the gut.
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8. ⭐ GSE284620 抗原选择的肠道 IgA 由 IgG1 生发中心 B 细胞 (GEX) 产生

• ✍️ 作者：未知作者
• 🏷️ 关键词：antigen、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Emily Siniscalco ; Hailong Meng ; Gisela Gabernet ; Stephanie Eisenbarth ; Steven KleinsteinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDetermine whether IgA B cells are derived from IgE B cells during allergy; understand whether IgE and IgA B cells induced during allergy share clonal relationships in the gut.
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9. ⭐ GSE309820 对 DICER1 综合征谱系可追踪小鼠模型的空间单细胞转录组分析揭示肿瘤发育层级 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、RNA-seq、spatial
• 📝 描述：Contributors : Felix K Kommoss ; Joyce Zhang ; Branden J Lynch ; Shary Y Chen ; Janine Senz ; Yana Moscovitz ; Lesley A Hill ; Wilder Scott ; Jonathan Bush ; Kenneth Chen ; Andrew Roth ; Andreas von Diemling ; William Foulkes ; Gregg B Morin ; Michael Underhill ; Yemin Wang ; David G HuntsmanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDICER1 syndrome predisposes children and young adults to tumor development across various organs. Most of these cancers are sarcomas, which uniquely express the RNase IIIb domain-deficient form of DICER1 and exhibit histological and molecular similarities regardless of their anatomical origins. To uncover their cellular origin and developmental hierarchy, we established a lineage-traceable genetically engineered mouse model allowing for controlled activation of Dicer1 mutations in Hic1+ mesenchymal stromal cells. This resulted in the development of renal tumors closely mirroring human DICER1 sarcoma histologically and molecularly. Spatial single-cell transcriptomics analysis revealed that a Hic1+Pdgfra+Dpt+Pi16+ fibroblastic progenitor population, corresponding to universal fibroblasts of steady-state kidneys, exhibits the capability to undergo rhabdomyoblastic differentiation or transition into proliferative sarcomatous cells. Investigation of patient samples identified analogous cell states and developmental trajectories. This study uncovers a fibroblastic origin for DICER1 sarcoma and provides a faithful model for future mechanistic and translational investigation.
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10. ⭐ GSE288990 对 DICER1 综合征谱系可追踪小鼠模型的空间单细胞转录组分析揭示肿瘤发育层级 [scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、scRNA、spatial
• 📝 描述：Contributors : Felix K Kommoss ; Joyce Zhang ; Branden J Lynch ; Shary Chen ; Janine Senz ; Yana Moscovitz ; Lesley Hill ; Ross Scott ; Jonathan Bush ; Andrew Roth ; Kenneth Chen ; Gregg Morin ; Andreas von Diemling ; Tully Underhill ; William Foulkes ; David G HuntsmanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDICER1 syndrome predisposes children and young adults to tumor development across various organs. Most of these cancers are sarcomas, which uniquely express the RNase IIIb domain-deficient form of DICER1 and exhibit histological and molecular similarities regardless of their anatomical origins. To uncover their cellular origin and developmental hierarchy, we established a lineage-traceable genetically engineered mouse model allowing for controlled activation of Dicer1 mutations in Hic1+ mesenchymal stromal cells. This resulted in the development of renal tumors closely mirroring human DICER1 sarcoma histologically and molecularly. Spatial single-cell transcriptomics analysis revealed that a Hic1+Pdgfra+Mfap4+ fibroblastic progenitor population, corresponding to perivascular universal fibroblasts of steady-state kidneys, exhibits the capability to undergo rhabdomyoblastic differentiation or transition into proliferative sarcomatous cells. Investigation of patient samples identified analogous cell states and developmental trajectories. This study uncovers a fibroblastic origin for DICER1 sarcoma and provides a faithful model for future mechanistic and translational investigation.
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1. ⭐ 可操作的转录组——将RNA测序纳入精准肿瘤学的框架

• ✍️ 作者：未知作者
• 🏷️ 关键词：oncology、regex:onco(logy|logist|gene|genic)、sequencing、transcriptome
• 📝 描述：RNA sequencing enables detection of actionable gene expression changes, fusions, and signatures that extend beyond DNA testing, supporting more precise and personalized cancer care…
• 🔗 查看原文

2. ⭐ 日常生活中常见的化学物质正在悄无声息地损害肠道有益菌群。

• ✍️ 作者：未知作者
• 🏷️ 关键词：bacteria、regex:bacter(ia|ial|ium)、gut、regex:gut(-?microbiome)?
• 📝 描述：A large study has revealed that dozens of widely used chemicals can damage beneficial gut bacteria. Many of these substances, found in pesticides and everyday industrial products, were never thought to affect living organisms at all. When gut bacteria are stressed by these chemicals, some may also become resistant to antibiotics. The research raises new questions about how chemical exposure could be influencing human health behind the scenes.
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3. ⭐ 揭示毛发衰老的奥秘——基于单细胞和时空测序的人类毛囊衰老分子图谱

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging、sequencing、single-cell
• 📝 描述：RNA sequencing reveals cell-type–specific transcriptional changes in aging human scalp, linking stem cell decline, altered signaling, and inflammatory stress to hair loss and graying…
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4. ARRP-seq——一种新的测序方法，可提高癌症中罕见和新型基因融合的检测率。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、sequencing
• 📝 描述：RNA sequencing with anchored random reverse primers improves detection of rare and novel gene fusions, enabling more sensitive and cost-effective cancer diagnostics…
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5. 人类智慧的秘密？或许就在我们的直觉里。

• ✍️ 作者：未知作者
• 🏷️ 关键词：gut、regex:gut(-?microbiome)?
• 📝 描述：New research shows gut bacteria can directly influence how the brain develops and functions. When scientists transferred microbes from different primates into mice, the animals’ brains began to resemble those of the original host species. Microbes from large-brained primates boosted brain energy and learning pathways, while others triggered very different patterns. The results suggest gut microbes may have played a hidden role in shaping the human brain—and could influence mental health.
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6. 科学家发现一种隐藏的开关，它能帮助癌细胞增殖。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：A protein once thought to simply help cancer cells avoid death turns out to do much more. MCL1 actively drives cancer metabolism by controlling the powerful mTOR growth pathway, tying survival and energy use together. This insight explains why MCL1-targeting drugs can be effective—but also why they sometimes damage the heart. Researchers have now identified a way to reduce that risk, potentially unlocking safer cancer therapies.
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• GSE315024 组蛋白乳化通过调节胚胎基因组激活来维持牛早期胚胎发育 [scRNA-Seq]
• GSE285928 从免疫和代谢角度评价牛蒡苷促进灭活传染性造血器官坏死病毒疫苗的功效
• GSE310729 年龄无关且可靶向的转录因子网络调控衰老人类 CD8+ T 细胞衰老 [RNA-seq_RNAi_TFs]
• GSE310625 年龄无关且可靶向的转录因子网络调控衰老人类 CD8+ T 细胞衰老 [RNA-seq_inhibitors]
• GSE310393 年龄无关且可靶向的转录因子网络调控衰老人类 CD8+ T 细胞衰老 [ATAC-seq_inhibitors]
• GSE310384 年龄无关且可靶向的转录因子网络调控衰老人类 CD8+ T 细胞衰老 [CUT&Tag]
• GSE305385 脂联素调节雄性小鼠肝脏昼夜转录组和能量代谢
• GSE281517 PARP抑制剂的溶酶体隔离驱动卵巢癌中的异质性积累以提高疗效[RNA-seq]
• GSE289001 对 DICER1 综合征谱系可追踪小鼠模型的空间单细胞转录组分析揭示肿瘤发育层级 [Xenium]
• GSE315451 血液表观遗传不稳定性与人类衰老和疾病相关 [MDS_CML]
• GSE315367 血液表观遗传不稳定性与人类衰老和疾病相关 [纵向_AML]
• GSE315366 血液表观遗传不稳定性与人类衰老和疾病相关 [心源性休克]
• GSE291899 厌氧真菌和链延长细菌的共培养物能稳定地将木质纤维素转化为丁酸和己酸
• GSE287728 BRD2 连接 TFIID 和组蛋白乙酰化在转录起始中的作用 [ChIP-Seq]
• GSE287723 BRD2 连接 TFIID 和组蛋白乙酰化在转录起始中的作用 [RNA-Seq]
• GSE305091 Tet2 调节糖尿病中的内质网应激反应、β 细胞死亡和自身免疫 [scRNA-seq]
• GSE302830 五因子（M5）处理对角质形成细胞系HaCaT转录组的影响
• GSE296953 PIONEER试验结果：一项旨在评估孕激素受体（PR）激动剂甲地孕酮联合来曲唑治疗早期雌激素受体（ER）阳性乳腺癌的“机会窗口”试验：利用ER-PR相互作用
• GSE295594 高度动态的硬脑膜窦支持脑膜免疫
• GSE291294 通过改变干细胞 RNA 调控来遗传对克隆造血的抵抗力 [scRNA-seq]
• GSE291293 通过改变干细胞 RNA 调控来遗传对克隆造血的抵抗力 [bulk RNA-seq]
• GSE283879 ATAD2 驱动 HIRA/组蛋白 H3.3 依赖的染色质动力学
• GSE277943 ATAD2 驱动 HIRA/组蛋白 H3.3 依赖的染色质动力学
• GSE315594 交配调节雌性果蝇中肠的生长、代谢和消化效率
• GSE311209 比较转录组分析揭示了胸膜肺母细胞瘤和先天性肺气道畸形 II 的不同发病机制
• GSE311205 比较转录组分析揭示了胸膜肺母细胞瘤和先天性肺气道畸形不同的发病机制 I
• GSE302290 肠道上皮绒毛化由Foxl1驱动
• GSE292273 MECOM：AR驱动的耐药性去势抵抗性前列腺癌领域的新成员
• GSE225125 正常和恶性未成熟 T 细胞中 Zmiz1 依赖的天然干细胞转录回路 [RNA-seq]
• GSE315604 中性粒细胞和巨噬细胞在急性肺损伤中阶段特异性免疫重塑的动态变化
• GSE315492 Phillygenin抑制组蛋白乳酸化修饰的NF-κB，从而减轻脓毒症引起的急性肺损伤
• GSE300674 微RNA-146a通过抑制CCL5来抵抗肝细胞癌
• GSE245850 线粒体复合物 I 的基因拯救逆转了昼夜节律突变小鼠的代谢缺陷
• GSE315646 人源化和电荷优化的CSPG4特异性CAR-T细胞显示出对头颈部鳞状细胞癌的增强疗效
• GSE315628 单细胞数据对 FGFR3 驱动的基因调控网络分析揭示了 p63 在腔内膀胱肿瘤中的促肿瘤作用
• GSE315569 适体修饰的5-氟尿嘧啶包封金属有机框架纳米药物通过过氧化诱导细胞死亡增强结直肠癌的治疗
• GSE315365 TGF-β刺激的小鼠原代心脏成纤维细胞的转录组分析
• GSE315188 毕赤酵母菌株转录组学分析，这些菌株经基因工程改造后可提高支链氨基酸的产量
• GSE309688 办公时间内的自然光照可改善血糖控制和全身底物代谢
• GSE308141 胚胎衍生蛋白（PDI、CAPG、PSAT1）在有胎盘哺乳动物中高度保守，并改变子宫内膜上皮细胞（牛和人）的转录组
• GSE297796 转座元件增强转录组可塑性的制图和机制
• GSE287724 BRD2 连接 TFIID 和组蛋白乙酰化在转录起始中的作用 [PRO-Seq]
• GSE286039 多组学图谱揭示细胞毒性表型和 ROS 相关的代谢静止是 CTL 耐药 HIV 感染 CD4+ T 细胞的关键特征
• GSE285955 交感神经支配调节骨骼肌的代谢可塑性
• GSE285787 DNA损伤驱动植物DNA甲基化的产生和进化
• GSE263042 scRNA-seq 评估抗 IL6R 和抗 OSM 在纤维化肺病模型消退期（第 24 天）的影响 [NGS4659]
• GSE263041 scRNA-seq 评估抗 IL6R 和抗 OSM 在纤维化肺病模型中纤维化期间（第 14 天）的影响 [NGS4272]
• GSE262966 确定抗IL6和抗OSM对纤维化肺病模型中巨噬细胞和成纤维细胞生物学的影响[NGS3341]