详细内容（前10条）

1. ⭐ GSE313299 CITE-seq 分析抗 IL10R 治疗小鼠肠道引流淋巴结中的树突状细胞，并采用 LIPSTIC 标记 Akkermansia muciniphila 呈递细胞

• ✍️ 作者：未知作者
• 🏷️ 关键词：lymph、regex:lymph(o|atic)?、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Shaina L Carroll ; Andrew Ly ; Ashley K Liu ; Maria C Canesso ; Gabriel D Victora ; Daniel Mucida ; Gregory M BartonSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusCommensal-specific T cells contribute to tissue homeostasis within the intestines, and can influence disease pathology in the context of both cancer and autoimmunity. Antigen presenting cells play a critical role in influencing the differentiation of these T cells, but how the complex network of antigen presenting cells in the intestines drives commensal-specific T cells towards a variety of differentiation trajectories remains poorly understood. We examined the priming of T cells specific for the intestinal commensal bacterial species Akkermansia muciniphila, using the LIPSTIC system combined with single-cell RNA-sequencing to identify the APCs that prime A. muciniphila-specific T cells both at homeostasis when it elicits T follicular helper cells, and during inflammation, when it also elicits TH17 and TH1 cells. We found that A. muciniphila-specific T cells were primed by several transcriptionally distinct subpopulations of migratory cDC2s in both contexts. The identity of these subpopulations did not change with inflammation, but the distribution of presentation across the subpopulations shifted, with increased presentation by inflammatory cDC2s favoring TH1 and TH17 polarization. These results reveal how distinct T cell differentiation trajectories can be determined through varied interactions with multiple, functionally distinct subpopulations of APCs.
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2. ⭐ GSE313298 利用 LIPSTIC 标记 Akkermansia muciniphila 呈递细胞，对稳态下肠道引流淋巴结中的树突状细胞进行 CITE-seq 分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：lymph、regex:lymph(o|atic)?、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Shaina L Carroll ; Andrew Ly ; Ashley K Liu ; Maria C Canesso ; Gabriel D Victora ; Daniel Mucida ; Gregory M BartonSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusCommensal-specific T cells contribute to tissue homeostasis within the intestines, and can influence disease pathology in the context of both cancer and autoimmunity. Antigen presenting cells play a critical role in influencing the differentiation of these T cells, but how the complex network of antigen presenting cells in the intestines drives commensal-specific T cells towards a variety of differentiation trajectories remains poorly understood. We examined the priming of T cells specific for the intestinal commensal bacterial species Akkermansia muciniphila, using the LIPSTIC system combined with single-cell RNA-sequencing to identify the APCs that prime A. muciniphila-specific T cells both at homeostasis when it elicits T follicular helper cells, and during inflammation, when it also elicits TH17 and TH1 cells. We found that A. muciniphila-specific T cells were primed by several transcriptionally distinct subpopulations of migratory cDC2s in both contexts. The identity of these subpopulations did not change with inflammation, but the distribution of presentation across the subpopulations shifted, with increased presentation by inflammatory cDC2s favoring TH1 and TH17 polarization. These results reveal how distinct T cell differentiation trajectories can be determined through varied interactions with multiple, functionally distinct subpopulations of APCs.
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3. ⭐ GSE311168 单细胞转录组分析揭示新生小鼠肠上皮酮体合成与形态发生特征之间的关联 II

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、transcriptome、regex:intestin(e|al)
• 📝 描述：Contributors : Kyoko Mastuki ; Taiki Sakaguchi ; Kiyoshi TakedaSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusThe intestinal epithelium undergoes robust maturation postnatally, yet its early-life characteristics remain poorly understood. Using single-cell RNA sequencing, we analyzed intestinal epithelial cells from neonatal and juvenile mice reared under both specific pathogen-free and germ-free conditions. Among the various cell types comprising the intestinal epithelia, we found that enterocytes, in particular, exhibit markedly different features between these stages. Enterocytes of neonatal mice show reduced expression of secretory host defense-related genes independently of microbial colonization. Conversely, neonatal enterocytes display upregulation of ketogenesis-related genes, which correlates with high expression of genes contributing to cell morphogenesis rather than serving primarily as an energy source. These findings provide novel insights into early-life maturation of intestinal epithelia offering implications for understanding neonatal intestinal pathologies.
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4. ⭐ GSE296272 单细胞转录组分析揭示新生小鼠肠上皮酮体合成与形态发生特征之间的关联

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、transcriptome、regex:intestin(e|al)
• 📝 描述：Contributors : Kyoko Mastuki ; Taiki Sakaguchi ; Kiyoshi TakedaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe intestinal epithelium undergoes robust maturation postnatally, yet its early-life characteristics remain poorly understood. Using single-cell RNA sequencing, we analyzed intestinal epithelial cells from neonatal (10-day-old) and juvenile (21-day-old) mice reared under both specific pathogen-free and germ-free conditions. Among the various cell types comprising the intestinal epithelia, we found that enterocytes, in particular, exhibit markedly different features between these stages. Enterocytes of neonatal mice show reduced expression of secretory host defense-related genes independently of microbial colonization. These genes are upregulated in juvenile enterocytes in a microbiota-dependent manner. Conversely, neonatal enterocytes display upregulation of ketogenesis-related genes, which correlates with high expression of genes contributing to cell morphogenesis rather than serving primarily as an energy source. These findings provide novel insights into early-life maturation of intestinal epithelia offering implications for understanding neonatal intestinal pathologies.
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5. ⭐ GSE295628 咪康唑通过调节脂质代谢来调控肺泡巨噬细胞极化，从而减轻LPS诱导的肺部炎症

• ✍️ 作者：未知作者
• 🏷️ 关键词：macrophage、inflammation、metabolism
• 📝 描述：Contributors : Yicheng Xie ; Jian ShenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMiconazole (MCZ), an antifungal agent with anti-inflammatory potential, was evaluated in a lipopolysaccharide (LPS)-induced mouse model of lung inflammation.MCZ reduced inflammatory cell infiltration, suppressed the expression of inflammatory factors, and alleviated lung injury.Single-cell RNA sequencing revealed that MCZ decreased pro-inflammatory alveolar macrophages (AM1) and increased anti-inflammatory AM2 subsets, accompanied by a shift from lipid synthesis to lipid catabolism.MCZ also disrupted the interactions between inflammatory cells in vitro and regulated the polarization of macrophages by promoting the expression of CD206 and reducing the expression of CD86 and PLIN3.These findings highlight MCZ as a potential therapeutic agent for pulmonary inflammation via lipid metabolic reprogramming and immune modulation.
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6. ⭐ GSE285901 PSY 通过靶向致癌信号传导和代谢重编程抑制结直肠癌进展

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、metabolic、regex:onco(logy|logist|gene|genic)
• 📝 描述：Contributors : Sanghee Han ; Seok-Geun LeeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBackground: Colorectal cancer (CRC) is a leading cause of cancer-related mortality, driven by dysregulated oncogenic signaling and metabolic reprogramming. PSY, an herbal formula derived from Patriniae Radix, Coix Seed, and Mori Cortex Radicis, has shown potential anti-cancer effects, but its mechanisms in CRC remain unclear. Purpose: PSY’s multi-targeted effects on oncogenic signaling, metabolic pathways, and inflammation in CRC. Materials and Methods: Transcriptomic profiling (RNA sequencing), in vitro assays, and in vivo xenograft models were used to elucidate PSY’s mechanisms. Metabolic profiling via LC-MS/MS and serum lipid analysis were performed to assess its impact on lipid metabolism. Results: PSY activated the PERK/eIF2α/ATF4 stress pathway and suppressed PI3K/Akt/mTOR signaling, inducing apoptosis and inhibiting CRC cell proliferation. Xenograft models showed significant tumor growth suppression and reduced proliferation (Ki67) and inflammation markers (COX2, p-STAT3). Metabolic profiling revealed reduced cholesterol and fatty acid biosynthesis, including arachidonic acid, correlating with COX2 downregulation. Serum LDL and HDL levels decreased, with an increased LDL/HDL ratio. Conclusion: PSY demonstrates potential as a multi-targeted agent by disrupting oncogenic signaling, lipid metabolism, and inflammation in CRC. These findings support its further exploration for clinical applications.
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7. GSE292917 光呼吸与甲酸作为一碳源的 DNA 甲基化有关 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、methylation
• 📝 描述：Contributors : Valentin Hankofer ; Martin GrothSeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thalianaDNA methylation is a key epigenetic modification regulating genome organization, stability, and gene expression. Stable DNA methylation critically relies on methyl groups provided through folate-mediated one-carbon (C1) metabolism, yet the origin and regulation of C1 supply remain elusive. Here we demonstrate that photorespiration serves as a major C1 source for DNA methylation in Arabidopsis. We show that C1 from formate, a photorespiratory byproduct, is incorporated into 5-methyl-cytosine via the reductive cytosolic folate pathway. This occurs predominantly during the day, negatively regulating serine utilization as alternative C1 source. Consequently, suppression of photorespiration under elevated CO₂ levels alters the DNA methylation landscape, an effect exacerbated when regulation of C1 metabolism by the formate-dependent pathway is impaired. Thus, our findings link the fundamental metabolic process of photorespiration to epigenetic stability, highlighting how rising atmospheric CO₂ levels can induce DNA methylation changes.
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8. GSE282881 利用 SCITO-seq2 对健康人外周血单核细胞进行超高通量单细胞转录组和表位分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：single-cell、transcriptome
• 📝 描述：Contributors : Su-Hyeon Lee ; Bo-yeong Jin ; Murim Choi ; Byungjin HwangSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Homo sapiensThis study presents comprehensive single-cell transcriptome and surface protein profiling using SCITO-seq2, an enhanced platform that improves the recovery of unique transcripts while enabling simultaneous detection of surface proteins. The analysis was conducted in two phases: first, using healthy donor PBMCs to validate technical performance, and second, by comparing samples from healthy controls, patients with childhood systemic lupus erythematosus (cSLE), and patients with CTLA4 haploinsufficiency and autoimmune infiltration (CHAI). These results demonstrate the platform’s applicability to both normal and disease states.
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9. GSE315350 微生物组来源的吲哚-3-乳酸通过芳烃受体通路减弱痤疮丙酸杆菌诱导的炎症

• ✍️ 作者：未知作者
• 🏷️ 关键词：inflammation、pathway
• 📝 描述：Contributors : Sang Gyu Lee ; Nam Hao Chau ; Seoyoon Ham ; Yujin Baek ; Ngoc Ha Nguyen ; Young In LeeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAcne vulgaris is a chronic inflammatory dermatosis where conventional therapies of-ten face limitations in efficacy and safety, necessitating the development of microbi-ome-targeted interventions. This study investigated the immunomodulatory potential of microbiome-derived tryptophan metabolites as a novel therapeutic strategy for Cutibacterium acnes-induced inflammation, focusing on the aryl hydrocarbon receptor (AHR) pathway. We evaluated indole-3-lactic acid (ILA), indole-3-acrylic acid (IAA), and indole-3-propionic acid (IPA) in comparison to tapinarof, utilizing C. ac-nes-stimulated human epidermal keratinocytes and a C. acnes-induced acne mouse model. In vitro, ILA and IPA significantly suppressed C. acnes-driven inflammatory mediators, including TNF-α, IL-1β, and COX2, whereas IAA demonstrated limited ef-ficacy. In vivo, ILA treatment exhibited superior therapeutic activity, markedly reduc-ing inflammatory cell infiltration, epidermal hyperplasia, and IL-1β expression. Tran-scriptomic analysis confirmed that ILA attenuates inflammatory signaling (e.g., IL-17 and TNF pathways) while upregulating AHR-responsive genes such as CYP1A1 and CYP1B1. Collectively, these findings establish ILA as a potent postbiotic that mitigates cutaneous inflammation through selective activation of the AHR. Future studies should prioritize the clinical translation of ILA-based topical fomulations, with rigorous evaluation of their efficacy and safety in well-designed human trials, to support their development as a non-antibiotic therapeutic alternative for acne management.
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10. GSE315348 瘦素激活的脑、棕色脂肪组织和心脏之间的串扰驱动缺血再灌注损伤后的心脏保护作用 [RNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cardiac、RNA-seq
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Rattus norvegicusCardiovascular disease remains the leading cause of death worldwide, with coronary artery disease being the primary contributor. Our recent studies suggest that activation of leptin receptors (LepRs) in the brain can improve cardiac function following myocardial infarction. However, the mechanism by which this cardioprotective effect is transmitted from the brain to the heart remains unclear. We hypothesize that brain LepR activation stimulates brown adipose tissue (BAT) to secrete extracellular vesicles (EVs) enriched with cardioprotective factors. These EVs may safeguard the heart by modulating cardiac mitochondrial function and collagen deposition. Our findings indicate that BAT ablation or BAT sympathetic denervation diminishes the cardioprotective effects of brain LepR activation. We also observed an increased concentration of EVs within BAT of rats treated with ICV leptin compared to vehicle-treated controls, an effect abolished by BAT denervation. Furthermore, knockdown of Rab27a in BAT reduced the cardioprotective benefits of brain LepR activation. MicroRNA (miR)-29c-3p was identified as a cargo of leptin-stimulated BAT-derived EVs and appears to play a key role in mitigating cardiac fibrosis after IR injury in leptin-treated animals. Thus, activation of LepR in the brain protects the heart after IR injury via sympathetic mediated BAT-derived EVs enriched with miR-29c-3p.
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1. 一种更智能的乳腺癌筛查方法正在兴起。

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：A groundbreaking study shows that breast cancer screening works better when it’s personalized. Instead of annual mammograms for all, women were screened based on genetics, health history, and lifestyle factors. This approach reduced advanced cancers without increasing risk for those screened less often. Most women preferred the personalized model, hinting at a major shift in future screening guidelines.
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• GSE315009 RNA-seq 分析暴露于细菌 PNGase 酶的秀丽隐杆线虫
• GSE314910 心脏结节病的空间转录组特征
• GSE313858 软组织癌患者接受新辅助 BO-112 和低分割放射治疗后纵向肿瘤样本的空间转录组数据
• GSE307691 干扰素刺激基因 ISG20 在原代人类成纤维细胞中诱导先天免疫防御特征 [RNA-Seq]
• GSE304187 RNA-seq 分析年轻小鼠与老年小鼠的肺组织
• GSE304185 Calumenin 通过促进波形蛋白稳态来预防成纤维细胞衰老和肺老化
• GSE300768 Clariom D Pico基因芯片检测酸中毒适应和对照PANC1胰腺癌细胞
• GSE296527 基础 IFNλ2/3 信号传导（而非 IFNλ1 信号传导）足以控制肠上皮细胞中的病毒感染
• GSE294253 HFL-1 细胞过表达 GFP、GFP-ERG 或 GFP-ERG-ΔIDR13 的 RNA-seq
• GSE312961 吴茱萸生物碱在体内外抑制胆囊癌的发生和发展
• GSE292915 光呼吸与甲酸作为一碳源的 DNA 甲基化有关 [BiSulfite-seq]
• GSE277735 肝脏特异性SelO敲除对正常饮食小鼠肝脏转录组的影响
• GSE275692 CAR-中性粒细胞在体内产生用于治疗胶质瘤
• GSE315389 基于FACS的全基因组CRISPR筛选平台鉴定CD47的调节因子
• GSE315349 瘦素激活的脑、棕色脂肪组织和心脏之间的串扰驱动缺血再灌注损伤后的心脏保护作用 [miRNA-seq]
• GSE315053 S-1 联合西达米德和恩伐利单抗作为晚期和转移性胰腺癌二线治疗方案（P-henomS/SCOG-P002）：一项单臂、探索性、多中心 II 期试验
• GSE313859 scRNA-seq 数据来自接受新辅助 BO-112 和低分割放射治疗的软组织癌患者
• GSE313668 Notch信号对TAF6delta驱动的转录组的影响。
• GSE310758 转录组分析揭示橡胶叶片在感染微孢子丝孢菌后植物激素反应的参与
• GSE307708 干扰素刺激基因 ISG20 在原代人成纤维细胞中诱导先天免疫防御特征
• GSE307692 干扰素刺激基因 ISG20 诱导原代人类成纤维细胞的先天免疫防御特征 [SLAM-seq]
• GSE300142 肿瘤酸中毒重塑糖萼以控制脂质清除和铁死亡