详细内容（前10条）

1. ⭐ GSE314254 优化的多药疗法重塑肿瘤微环境并增强微卫星稳定型结直肠癌的抗肿瘤免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、immunity、tumor microenvironment
• 📝 描述：Contributors : Pablo H Lopez ; Patrycja Nowak-SliwinskaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusFour low-dose tyrosine kinase inhibitor (TKI)–based ODCs were evaluated in murine AKP CRC organoids, co-culture systems with tumor-associated endothelial cells and immune cells, and in vivo syngeneic models. Among these, ODCDLD1 (regorafenib, vemurafenib, erlotinib HCl, selumetinib) showed the most potent inhibition of tumor organoid viability (∼90%), promoted leukocyte infiltration in co-cultures, and significantly upregulated endothelial adhesion molecules (ICAM-1, VCAM-1, E-selectin). In vivo, ODCDLD1 reduced tumor growth comparably to oxaliplatin but with a favorable toxicity profile. Immunofluorescence analysis revealed that both ODCDLD1 and oxaliplatin significantly reduced the population of proliferating cancer cells. Furthermore, ODCDLD1-based treatment recapitulated transcriptomic findings by showing a significant reduction in proliferating myofibroblasts, a key stromal component. Bulk RNA sequencing revealed that ODCDLD1, especially when combined with anti-PD-1, induced an immunologically “hot” tumor microenvironment characterized by enhanced immune cell recruitment, reduced fibroblast and stem-like cell signatures, and upregulation of endothelial markers.
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2. ⭐ GSE291419：基于双门控巨噬细胞-细菌激活平台的精准肿瘤免疫疗法

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、macrophage、regex:bacter(ia|ial|ium)、regex:immuno(logy|therapy|suppression)
• 📝 描述：Contributors : Lin Li ; Leyang Wu ; Zichun HuaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusImmunotherapy based on live microorganisms has shown promise in preclinical studies, but its clinical translation has been hampered by limited efficacy and innegligible toxicity. Here, we developed M-BLAST (Macrophage-Bacteria encapsulation Lytic Autoactivated Synergistic Therapeutics), a dual-gated macrophage-mediated bacterial tumor-targeted delivery and in situ activation system. M-BLAST incorporates density-regulated virulence-enhanced attenuated Salmonella strains as the therapeutic core, thermally-controlled GSDMD-N-expressing macrophages as the delivery vector, and copper selenide, a photothermal material, as a heat-shock “primer.” Following systemic administration, localized near-infrared irradiation at the tumor site triggers macrophage pyroptosis, ensuring rapid and complete bacterial release. This disrupts the immunosuppressive tumor microenvironment and elicits a widespread cascading antitumor response just like a “immune bomb”, while dual-gating design of bacterial density and heat-shock ensures safety by preventing off-target activation in non-tumor regions. M-BLAST promises to enhance the therapeutic utility of living engineered bacteria for cancer while ensuring safety.
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3. ⭐ GSE297247 妊娠期免疫激活通过表观遗传机制对突触和神经发育通路产生产前和产后影响（ChIP-seq）

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、ChIP-seq、epigenetic
• 📝 描述：Contributors : Bohan Zhu ; Gaoshan Li ; Chang LuSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusPrevious epidemiological research suggests that maternal immune activation (MIA) during early pregnancy is a significant risk factor for neurodevelopmental and psychiatric disorders in offspring. Epigenetic factors and chromatin-related phenomena remain largely plastic during the course of prenatal and early postnatal development, allowing a dynamic impact of environmental effects on access to genes and regulatory elements. In this study, we tested the effects of maternal infection with the mouse-adapted influenza A/WSN/33 (H1N1) virus as a MIA model. Additionally, to assess the prenatal and postnatal effects of MIA, we cross-fostered half of the neonatal mice immediately after birth.This study includes RNA-seq and ChIP-seq profiling (H3K27ac, H3K4me3) from the prefrontal cortex of MIA-exposed and control mice. RNA-seq and ChIP-seq data are submitted under separate Series and linked via sample annotations.
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4. ⭐ GSE297245 妊娠期免疫激活通过表观遗传机制对突触和神经发育通路产生产前和产后影响（RNA-seq）

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune、RNA-seq、epigenetic
• 📝 描述：Contributors : Bohan Zhu ; Gaoshan Li ; Chang LuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPrevious epidemiological research suggests that maternal immune activation (MIA) during early pregnancy is a significant risk factor for neurodevelopmental and psychiatric disorders in offspring. Epigenetic factors and chromatin-related phenomena remain largely plastic during the course of prenatal and early postnatal development, allowing a dynamic impact of environmental effects on access to genes and regulatory elements. In this study, we tested the effects of maternal infection with the mouse-adapted influenza A/WSN/33 (H1N1) virus as a MIA model. Additionally, to assess the prenatal and postnatal effects of MIA, we cross-fostered half of the neonatal mice immediately after birth.This study includes RNA-seq and ChIP-seq profiling (H3K27ac, H3K4me3) from the prefrontal cortex of MIA-exposed and control mice. RNA-seq and ChIP-seq data are submitted under separate Series and linked via sample annotations.
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5. ⭐ GSE284605 锌指和BTB结构域包含32 (Zbtb32) 转录因子促进癌症中CD8+ T细胞的分化和功能 [ChIP-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、T cell、ChIP-seq
• 📝 描述：Contributors : Birui Pan ; Ruifeng Li ; Xiaohong Zhao ; Chen DongSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusIn the tumor microenvironment (TME), “exhausted” CD8+ T cells are classified into progenitor (Tpex) and terminally exhausted (Ttex) populations. Tpex cells could be reinvigorated during immune checkpoint blockade (ICB) therapy. However, the mechanisms governing the differentiation of Tpex to Ttex remain not well understood. In this study, we identified that Zinc Finger and BTB Domain Containing 32 (Zbtb32) transcription factor, highly expressed in CD8+ Ttex subset, plays a critical role in regulating CD8+ T cells within tumors. Zbtb32, regulated by CD28 signaling, promotes the differentiation of CD8+ T cells into Ttex subset, enhancing their cytotoxicity, proliferation and anti-tumor capability. Importantly, we found a competitive DNA binding between Zbtb32 and Bcl6, another Zbtb family member promoting the Tpex program, especially in regulation of Id2 expression. Thus, our findings underscore the pivotal role of Zbtb32 in CD8+ T cell anti-tumor function, with implications in cancer immunotherapy.
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6. ⭐ GSE284604 锌指和BTB结构域包含32 (Zbtb32) 转录因子促进癌症中CD8+ T细胞的分化和功能 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、T cell、RNA-seq
• 📝 描述：Contributors : Birui Pan ; Xiaohong Zhao ; Ruifeng Li ; Chen DongSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn the tumor microenvironment (TME), “exhausted” CD8+ T cells are classified into progenitor (Tpex) and terminally exhausted (Ttex) populations. Tpex cells could be reinvigorated during immune checkpoint blockade (ICB) therapy. However, the mechanisms governing the differentiation of Tpex to Ttex remain not well understood. In this study, we identified that Zinc Finger and BTB Domain Containing 32 (Zbtb32) transcription factor, highly expressed in CD8+ Ttex subset, plays a critical role in regulating CD8+ T cells within tumors. Zbtb32, regulated by CD28 signaling, promotes the differentiation of CD8+ T cells into Ttex subset, enhancing their cytotoxicity, proliferation and anti-tumor capability. Importantly, we found a competitive DNA binding between Zbtb32 and Bcl6, another Zbtb family member promoting the Tpex program, especially in regulation of Id2 expression. Thus, our findings underscore the pivotal role of Zbtb32 in CD8+ T cell anti-tumor function, with implications in cancer immunotherapy.
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7. ⭐ GSE284603 锌指和BTB结构域包含32 (Zbtb32) 转录因子促进癌症中CD8+ T细胞的分化和功能 [ATAC-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、T cell、ATAC-seq
• 📝 描述：Contributors : Birui Pan ; Xiaohong Zhao ; Chen DongSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusIn the tumor microenvironment (TME), “exhausted” CD8+ T cells are classified into progenitor (Tpex) and terminally exhausted (Ttex) populations. Tpex cells could be reinvigorated during immune checkpoint blockade (ICB) therapy. However, the mechanisms governing the differentiation of Tpex to Ttex remain not well understood. In this study, we identified that Zinc Finger and BTB Domain Containing 32 (Zbtb32) transcription factor, highly expressed in CD8+ Ttex subset, plays a critical role in regulating CD8+ T cells within tumors. Zbtb32, regulated by CD28 signaling, promotes the differentiation of CD8+ T cells into Ttex subset, enhancing their cytotoxicity, proliferation and anti-tumor capability. Importantly, we found a competitive DNA binding between Zbtb32 and Bcl6, another Zbtb family member promoting the Tpex program, especially in regulation of Id2 expression. Thus, our findings underscore the pivotal role of Zbtb32 in CD8+ T cell anti-tumor function, with implications in cancer immunotherapy.
• 🔗 查看原文

8. GSE297440 用于即时病毒基因组测序的微反应器系统

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、genome
• 📝 描述：Contributors : Thomas M Hadlock ; Jacob Neice ; Chang LuSeries Type : OtherOrganism : Sus scrofa domesticusViral genome sequencing is critical for understanding viral biology, identifying strain mutations, and managing public health during pandemics. Although sequencing technologies have progressed over the years in terms of throughput and cost, a portable platform that integrates sample processing, library preparation, and sequencing remains critical for point-of-care viral sequencing that combats viral outbreaks in the field. In here, we demonstrate a semi-automated, field-deployable microreactor system to produce nanopore sequencing libraries from viral samples. The system integrates isothermal amplification, purification, and enzymatic processing within a closed-channel format, minimizing contamination risk and operational complexity. Using Senecavirus A (SVA) as a surrogate for high-consequence pathogens, we demonstrate the system’s ability to prepare sequencing libraries targeting three dispersed genomic regions comprising ~16% of the viral genome and identify single nucleotide variations with high confidence. Together with a portable nanopore sequencer, this microreactor offers a robust solution for decentralized genomic surveillance and rapid diagnostics in resource-limited or outbreak-prone environments.
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9. GSE295934 空间上不同的细胞和分子图谱决定三阴性乳腺癌的预后

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、spatially
• 📝 描述：Series Type : OtherOrganism : Homo sapiens ; synthetic constructThis SuperSeries is composed of the SubSeries listed below.
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10. GSE295927 空间上不同的细胞和分子景观决定了三阴性乳腺癌的预后[2]

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、spatially
• 📝 描述：Contributors : Kavitha Mukund ; Darya Veraksa ; David E Frankhouser ; Lixin Yang ; Jerneja Tomsic ; Raju Pillai ; Srijan Atti ; Zahra Mesrizadeh ; Daniel Schmolze ; Xio-Cheng Wu ; Mary-Anne LeBlanc ; Lucio Miele ; Augusto Ochoa ; Victoria Seewaldt ; Shankar SubramaniamSeries Type : OtherOrganism : Homo sapiens ; synthetic constructTriple-negative breast cancer is a prevalent breast cancer subtype with the lowest 5-year survival. Several factors contribute to its treatment response, but the inherent molecular and cellular tumor heterogeneity are increasingly acknowledged as crucial determinants. Here we report on the spatial and molecular heterogeneity underlying a retrospective, treatment-naïve group of women with differential prognoses (17 with >15 years survival- good prognosis (GPx) and 15 with <3 years survival-poor prognosis (PPx)) profiled using GeoMX® DSP. Analyses revealed that the state of epithelia and its microenvironment (TME) are transcriptionally distinct between the two groups. Invasive epithelia in GPx shows a significant increase in immune transcripts, with the TME exhibiting increased immune cell presence (via IF). PPx epithelia, in contrast, are more metabolically and translationally active, with the TME being more mesenchymal/fibrotic. Notably, pre-cancerous epithelia in PPx display a prescience of aggressiveness, as evidenced by increased EMT-signaling. We identify distinct epithelial gene signatures for PPx and GPx, that can, with high accuracy, classify samples at the time of diagnosis and are likely to inform therapy.
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1. 探索基因表达与衰老之间的联系

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Advanced RNA sequencing reveals how transcription elongation factors like NELF and ELOA regulate gene expression and senescence, linking transcription dynamics with aging and potential anti-aging targets…
• 🔗 查看原文

2. 科学家发现了一种减缓细胞内衰老的新方法。

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：A small tweak to mitochondrial energy production led to big gains in health and longevity. Mice engineered to boost a protein that helps mitochondria work more efficiently lived longer and showed better metabolism, stronger muscles, and healthier fat tissue. Their cells produced more energy while dialing down oxidative stress and inflammation tied to aging. The results hint that improving cellular power output could help slow the aging process itself.
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3. 揭开98%之谜：科学家们刚刚破解了与阿尔茨海默病相关的“垃圾DNA”密码

• ✍️ 作者：未知作者
• 🏷️ 关键词：Alzheimer
• 📝 描述：Researchers have revealed that so-called “junk DNA” contains powerful switches that help control brain cells linked to Alzheimer’s disease. By experimentally testing nearly 1,000 DNA switches in human astrocytes, scientists identified around 150 that truly influence gene activity—many tied to known Alzheimer’s risk genes. The findings help explain why many disease-linked genetic changes sit outside genes themselves. The resulting dataset is now being used to train AI systems to predict gene control more accurately.
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4. 每周抽出几个小时帮助他人或许可以延缓大脑衰老

• ✍️ 作者：未知作者
• 🏷️ 关键词：aging
• 📝 描述：Spending a few hours a week helping others may slow the aging of the brain. Researchers found that both formal volunteering and informal acts, like helping neighbors or relatives, were linked to noticeably slower cognitive decline over time. The benefits added up year after year and didn’t require a huge time commitment. Even modest, everyday helping packed a powerful mental payoff.
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• GSE295858 空间上不同的细胞和分子景观决定了三阴性乳腺癌的预后[1]
• GSE301299 多组学分析鉴定出内在的 Trp53 驱动的转移性乳腺癌亚型 [RNA-seq]
• GSE291580 受控的工程化巨噬细胞焦亡可通过释放溶瘤细菌和炎症信号实现原位双相抗肿瘤作用
• GSE313754 基质MTHFD2调控纤维化-炎症微环境，驱动肿瘤生长和免疫逃逸
• GSE297906 研究氧化低密度脂蛋白对 CD4+ T 细胞 H3K27ac 表观遗传标记的影响
• GSE295462 利用表面标志物鉴定捕获转移性三阴性乳腺癌中的活循环肿瘤细胞
• GSE235597 系统性诱导训练免疫可克服实体瘤的免疫抑制微环境。[scRNA-Seq]
• GSE233404 衰老依赖性EXOSC2失调在癌症中持续存在并具有依赖性
• GSE128468 PLPP1抑制乳腺癌肿瘤生长
• GSE314344 HIV抑制结核分枝杆菌感染的人类巨噬细胞中的Warburg代谢
• GSE311007 糖原合成酶2敲除斑马鱼肝脏葡萄糖代谢增强
• GSE292997 整合素β1标志着多发性硬化症中脑内抗体分泌细胞的前体
• GSE288602 动脉粥样硬化通过循环髓系细胞中的干扰素启动，促进 COVID-19 疾病中过度的免疫反应
• GSE279779 NFκB1缺陷与Flt3髓系白血病相关，通过RelA:p52和SOD2表达失调增强细胞自我更新能力
• GSE314460 高分辨率体内结合数据显示，TBP 在有或没有 TFIID 的情况下均可在 TATA 启动子处启动转录 [RNA-seq]
• GSE313708 核外泌体介导的 RNA 降解对于 Piwi 介导的转座子沉默至关重要 [RNA-seq]
• GSE313706 核外泌体介导的 RNA 降解对于 Piwi 介导的转座子沉默至关重要 [ChIP-seq]
• GSE309627 环二鸟苷酸通过靶向 PSMD3 抑制癌症转移，且该过程独立于 STING。
• GSE307538 母体免疫激活诱导恒河猴后代杏仁核中长期、细胞特异性的转录组变化
• GSE274431 核外泌体是 Piwi-piRNA 介导的共转录基因沉默所必需的 [ChIP-seq]
• GSE274430 核外泌体是 Piwi-piRNA 介导的共转录基因沉默所必需的 [RNA-seq]
• GSE314580 抑制脂质分解代谢决定禁食后的寿命 [禁食 RNA-Seq]
• GSE307511 阐明突变型 p53 驱动的乳腺癌中 DCIS 进展的协同遗传事件 [WGS]
• GSE307509 阐明突变型 p53 驱动的乳腺癌中 DCIS 进展的协同遗传事件 [WES]
• GSE301300 多组学分析鉴定出内在的 Trp53 驱动的转移性乳腺癌亚型 [WES]
• GSE297459 人类膀胱细胞空间分布图
• GSE278578 β2 肾上腺素能信号通过诱导 MAPK 依赖性 Il1b 转录刺激巨噬细胞早期 IL-1β 分泌 [ATAC-Seq]
• GSE278577 β2 肾上腺素能信号通过诱导 MAPK 依赖的 Il1b 转录刺激巨噬细胞早期 IL-1β 分泌 [RNA-Seq]
• GSE313751 人类耐受性树突状细胞亚型对细胞毒性 CD4+ T 细胞的诱导发挥不同的作用
• GSE310779 研究圈养南非猎豹（Acinonyx jubatus jubatus）的年龄、性别和疾病的表观遗传生物标志物
• GSE309759 葡萄糖代谢调控人类皮层细胞多样化和成熟
• 来自日内瓦癌症登记处的GSE303377结直肠癌队列
• GSE302999 慢性NK细胞活化导致功能障碍，形成类似组织驻留细胞的状态，这种状态由KLF2缺陷介导
• GSE297905 研究氧化低密度脂蛋白对CD4+ T细胞基因表达的影响
• GSE297904 研究氧化低密度脂蛋白 (oxLDL) 对 CD4+ T 细胞染色质可及性的影响
• GSE297058 遗传性慢性胰腺炎诱导的可塑性与突变型 Kras 协同作用，促进早期胰腺癌的发生
• GSE235598 系统性诱导训练免疫可克服实体瘤的免疫抑制微环境
• GSE235364 系统性诱导训练免疫可克服实体瘤的免疫抑制微环境。[scMULTIome]
• GSE112017 去泛素化酶 USP20 通过稳定 SLUG 促进乳腺癌转移