详细内容（前10条）

1. ⭐ GSE285427 PKM2/STAT1通路调控派氏淋巴集结内生发中心形成中幼稚B细胞的命运以及肠道黏膜免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：immunity、B cell、pathway、gut、regex:gut(-?microbiome)?
• 📝 描述：Contributors : Li Zeng ; Zhiyin HuangSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis study investigates the effects of high-protein diets on intestinal B cell development and their role in Crohn’s disease (CD) pathogenesis. Our results demonstrate that a high-protein diet (HPD) combined with low-dose dextran sulfate sodium (DSS) effectively models CD in juvenile mice, leading to disrupted B cell development in Peyer’s patches (PPs). This is characterized by a reduction in germinal center (GC) B cells and an increase in plasma cells in PPs, accompanied by downregulation of the PKM2/STAT1 signaling pathway in B cells, as validated through single-cell RNA sequencing (scRNA-seq), BCR sequencing (scBCR-seq) and flow cytometry.
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2. ⭐ GSE313761 单细胞筛选鉴定出 ADAM12 为成纤维细胞检查点，阻碍抗肿瘤免疫——Adam12CKO 小鼠模型 CD45- 细胞的 scRNA-seq

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、single-cell、scRNA
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusClinical trials targeting cancer-associated fibroblasts (CAFs)—crucial pro-tumoral factors in cancer—have almost all failed. This may be ascribed to their intrinsic functional plasticity and the opaque regulatory circuits underlying their heterogeneous phenotypes within tumors. We address these by developing a systematic screening approach for patient-derived fibroblasts using complementary CRISPR interference (CRISPRi) and activation (CRISPRa)-based perturb-seq. An anti-tumoral interferon (IFN)-I response-associated program is identified as the primary antagonism axis counteracting TGFβ-driven pro-tumoral myofibroblast activation. ADAM12 emerges as a molecular checkpoint mediating this relationship. Its ablation elicits IFN-I responsive programs, reconfigures myofibroblast population structures into progenitor-like states, revitalizes T cell-based immune responses, and induces tumor rejection across various murine models. Further combined with human genomics data analysis, our findings position ADAM12 as a potential target for fibroblasts, paving the way for actionable therapeutic interventions.
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3. ⭐ GSE278983 单细胞筛选鉴定出ADAM12为成纤维细胞检查点，阻碍抗肿瘤免疫——小鼠KPC模型的scRNA-seq

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、single-cell、scRNA
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusClinical trials targeting cancer-associated fibroblasts (CAFs)—crucial pro-tumoral factors in cancer—have almost all failed. This may be ascribed to their intrinsic functional plasticity and the opaque regulatory circuits underlying their heterogeneous phenotypes within tumors. We address these by developing a systematic screening approach for patient-derived fibroblasts using complementary CRISPR interference (CRISPRi) and activation (CRISPRa)-based Perturb-seq . An anti-tumoral interferon (IFN)-I response-associated program is identified as the primary antagonism axis counteracting TGFβ-driven pro-tumoral myofibroblast activation. ADAM12 emerges as a molecular checkpoint mediating this relationship. Its ablation elicits IFN-I responsive programs, reconfigures myofibroblast population structures into progenitor-like states, revitalizes T cell-based immune responses, and induces tumor rejection across various murine models. Further combined with human genomics data analysis, our findings position ADAM12 as a potential target for fibroblasts, paving the way for actionable therapeutic interventions.
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4. ⭐ GSE278980 单细胞筛选鉴定出 ADAM12 为成纤维细胞检查点，阻碍抗肿瘤免疫——C57BL/6 小鼠模型的 RNA 测序

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、RNA-seq、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusClinical trials targeting cancer-associated fibroblasts (CAFs)—crucial pro-tumoral factors in cancer—have almost all failed. This may be ascribed to their intrinsic functional plasticity and the opaque regulatory circuits underlying their heterogeneous phenotypes within tumors. We address these by developing a systematic screening approach for patient-derived fibroblasts using complementary CRISPR interference (CRISPRi) and activation (CRISPRa)-based Perturb-seq . An anti-tumoral interferon (IFN)-I response-associated program is identified as the primary antagonism axis counteracting TGFβ-driven pro-tumoral myofibroblast activation. ADAM12 emerges as a molecular checkpoint mediating this relationship. Its ablation elicits IFN-I responsive programs, reconfigures myofibroblast population structures into progenitor-like states, revitalizes T cell-based immune responses, and induces tumor rejection across various murine models. Further combined with human genomics data analysis, our findings position ADAM12 as a potential target for fibroblasts, paving the way for actionable therapeutic interventions.
• 🔗 查看原文

5. ⭐ GSE278975 单细胞筛选鉴定出ADAM12是成纤维细胞检查点，可阻碍抗肿瘤免疫——共注射小鼠模型的scRNA-seq

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、single-cell、scRNA
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusClinical trials targeting cancer-associated fibroblasts (CAFs)—crucial pro-tumoral factors in cancer—have almost all failed. This may be ascribed to their intrinsic functional plasticity and the opaque regulatory circuits underlying their heterogeneous phenotypes within tumors. We address these by developing a systematic screening approach for patient-derived fibroblasts using complementary CRISPR interference (CRISPRi) and activation (CRISPRa)-based Perturb-seq . An anti-tumoral interferon (IFN)-I response-associated program is identified as the primary antagonism axis counteracting TGFβ-driven pro-tumoral myofibroblast activation. ADAM12 emerges as a molecular checkpoint mediating this relationship. Its ablation elicits IFN-I responsive programs, reconfigures myofibroblast population structures into progenitor-like states, revitalizes T cell-based immune responses, and induces tumor rejection across various murine models. Further combined with human genomics data analysis, our findings position ADAM12 as a potential target for fibroblasts, paving the way for actionable therapeutic interventions.
• 🔗 查看原文

6. ⭐ GSE278974 单细胞筛选鉴定出 ADAM12 为成纤维细胞检查点，阻碍抗肿瘤免疫——B16F10 肺黑色素瘤小鼠模型的 RNA-seq

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、RNA-seq、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusClinical trials targeting cancer-associated fibroblasts (CAFs)—crucial pro-tumoral factors in cancer—have almost all failed. This may be ascribed to their intrinsic functional plasticity and the opaque regulatory circuits underlying their heterogeneous phenotypes within tumors. We address these by developing a systematic screening approach for patient-derived fibroblasts using complementary CRISPR interference (CRISPRi) and activation (CRISPRa)-based Perturb-seq . An anti-tumoral interferon (IFN)-I response-associated program is identified as the primary antagonism axis counteracting TGFβ-driven pro-tumoral myofibroblast activation. ADAM12 emerges as a molecular checkpoint mediating this relationship. Its ablation elicits IFN-I responsive programs, reconfigures myofibroblast population structures into progenitor-like states, revitalizes T cell-based immune responses, and induces tumor rejection across various murine models. Further combined with human genomics data analysis, our findings position ADAM12 as a potential target for fibroblasts, paving the way for actionable therapeutic interventions.
• 🔗 查看原文

7. ⭐ GSE311439 脂质氧自由基防御通路及其表观遗传控制的鉴定 [ChIP-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：ChIP-seq、epigenetic、pathway
• 📝 描述：Contributors : Francisco S Mesquita ; Laurence Abrami ; Romain Forey ; Béatrice Kunz ; Charlène Raclot ; Lucie Bracq ; Danica Milovanovic ; Evarist Planet ; Olga Rosspopoff ; Filipe Martins ; Didier Trono ; Gisou van der GootSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensMembrane phospholipids are vulnerable to oxidative radicals, and uncontrolled lipid peroxidation affects cell viability. Cells have evolved quality control and defense mechanisms, of which the genetic regulation is not fully understood. Here, we identify what we have coined the Lipid Oxygen Radical Defense (LORD) pathway. It is epigenetically repressed by a complex comprising the KRAB-zinc finger protein ZNF354A, the scaffold protein KAP1/TRIM28, the histone methyltransferase SETDB1, and the transcriptional activator ATF2. Upon lipid peroxide accumulation, p38- and JNK-dependent phosphorylation of ATF2, KAP1/TRIM28, and ZNF354A leads to disassembly of the repressive complex, releasing ZNF354A from specific DNA loci and activating a network of protective genes, including NRF2 targets. The pathway affects the cellular sensitivity to oxidative stress and ferroptosis, revealing a previously uncharacterized layer of epigenetic control in lipid quality control and damage repair. This positions the LORD pathway as a promising therapeutic target for diseases linked to chronic inflammation, neurodegeneration and cancer.
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8. ⭐ GSE311436 脂质氧自由基防御通路及其表观遗传控制的鉴定 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：RNA-seq、epigenetic、pathway
• 📝 描述：Contributors : Francisco S Mesquita ; Evarist Planet ; Laurence Abrami ; Charlene Raclot ; Romain Forey ; Gisou van der GootSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensMembrane phospholipids are vulnerable to oxidative radicals, and uncontrolled lipid peroxidation affects cell viability. Cells have evolved quality control and defense mechanisms, of which the genetic regulation is not fully understood. Here, we identify what we have coined the Lipid Oxygen Radical Defense (LORD) pathway. It is epigenetically repressed by a complex comprising the KRAB-zinc finger protein ZNF354A, the scaffold protein KAP1/TRIM28, the histone methyltransferase SETDB1, and the transcriptional activator ATF2. Upon lipid peroxide accumulation, p38- and JNK-dependent phosphorylation of ATF2, KAP1/TRIM28, and ZNF354A leads to disassembly of the repressive complex, releasing ZNF354A from specific DNA loci and activating a network of protective genes, including NRF2 targets. The pathway affects the cellular sensitivity to oxidative stress and ferroptosis, revealing a previously uncharacterized layer of epigenetic control in lipid quality control and damage repair. This positions the LORD pathway as a promising therapeutic target for diseases linked to chronic inflammation, neurodegeneration and cancer.
• 🔗 查看原文

9. ⭐ GSE278990 单细胞筛选发现 ADAM12 是成纤维细胞检查点，可阻碍抗肿瘤免疫

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、immunity、single-cell
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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10. GSE293631 霸权 EWSR1::ETS 癌基因在尤文氏肉瘤中凌驾于核心调控回路机制之上 [ChIP-seq 2]

• ✍️ 作者：未知作者
• 🏷️ 关键词：regex:onco(logy|logist|gene|genic)、ChIP-seq
• 📝 描述：Contributors : Sandrine Grossetete ; Sakina Zaidi ; Karine Laud-Duval ; Didier Surdez ; Olivier DelattreSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensEwing sarcoma is an aggressive bone tumor of adolescence characterized by a hallmark EWSR1::FLI1 fusion oncogene. This chimeric protein drives tumorigenesis by reshaping transcriptional and epigenetic landscapes. However, how it is transcriptionally regulated and whether additional master transcription factors (MTFs) form a core regulatory circuit (CRC) in Ewing sarcoma remain unclear. Here, using an extensive panel of Ewing sarcoma cell lines and primary tumors, we mapped super-enhancers and identified strong enrichment of GGAA microsatellites, confirming their specificity to Ewing sarcoma as compared to other pediatric cancers and normal tissues. Integrating transcriptomic, epigenetic, 3D chromatin conformation, and dependency data, we predicted a set of MTFs potentially comprising a CRC. However, functional validation demonstrated that these MTFs neither establish auto-regulatory loops nor confer proliferative dependencies typical of CRC s in other pediatric tumors. Instead, EWSR1::FLI1 emerged as an “hegemonic” oncogene, regulating the expression of these MTFs without reciprocal regulation. Knockdown (KD) of EWSR1::FLI1 strongly reduced Ewing sarcoma cell proliferation and shifted H3K27ac profiles toward mesenchymal states, whereas silencing of individual or combined MTFs did not alter cell growth or EWSR1::FLI1 expression. These findings highlight the absence of a classical CRC in Ewing sarcoma and emphasize EWSR1::FLI1 as the dominant oncogene but also as a major vulnerability in this disease.
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1. 研究人员因基因组和RNA测序的变革性应用而获奖

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing、genome
• 📝 描述：Pengfei Liu, Ph.D., is recognized for advancing RNA sequencing and genome-based diagnostics, improving rare disease diagnosis…
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2. 利用RNA测序和因果模型将基因与性状联系起来

• ✍️ 作者：未知作者
• 🏷️ 关键词：sequencing
• 📝 描述：By combining genetic effect sizes with RNA sequencing based perturbation data, researchers map causal pathways from genes to cellular programs and human traits…
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3. 大麻化合物展现出对抗卵巢癌的意外功效

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer
• 📝 描述：Scientists have discovered that key compounds from cannabis—CBD and THC—show surprisingly strong effects against ovarian cancer cells. Used together, they slow cell growth, reduce colony formation, and may even block the cancer’s ability to spread. Even more promising, the treatment caused minimal harm to healthy cells and appears to work by restoring a disrupted signaling pathway that fuels tumor growth.
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4. 焦虑和失眠与关键免疫细胞数量急剧下降有关

• ✍️ 作者：未知作者
• 🏷️ 关键词：immune
• 📝 描述：Natural killer cells act as the immune system’s rapid-response team, but the stress of anxiety and insomnia may be quietly thinning their ranks. A study of young women in Saudi Arabia found that both conditions were linked to significantly fewer NK cells—especially the circulating types responsible for destroying infected or abnormal cells. As anxiety severity increased, NK cell levels dropped even further, suggesting a stress-driven weakening of immune defenses.
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• GSE293630 霸权 EWSR1::ETS 癌基因在尤文氏肉瘤中凌驾于核心调控回路机制之上 [ChIP-seq]
• GSE293629 霸权 EWSR1::ETS 癌基因在尤文氏肉瘤中凌驾于核心调控回路机制之上 [RNA-seq]
• GSE292538 KRAS依赖性内皮细胞代谢重编程作为脑动静脉畸形的治疗弱点
• GSE255078 断奶后喂食含半乳糖、葡萄糖或乳糖饮食的小鼠近端小肠刮取物的 RNA 测序
• GSE312888 表观转录组编辑筛选鉴定癌症中的功能性 m6A 位点 [RNA-Seq 22Rv1 M3 写入]
• GSE300690 神经元 Idol 基因缺失通过 APOE 受体改善阿尔茨海默病相关病理
• GSE298088 人类卵巢透明细胞癌PDX肿瘤的RNA测序
• GSE292092 错配修复缺陷驱动恶性进展并改变胶质母细胞瘤的肿瘤免疫微环境
• GSE280897 PUF60介导的剪接是三阴性乳腺癌的关键驱动因素[RNA-seq]
• GSE261507 具有时间分辨率的成年C57BL/6小鼠萘棒状细胞损伤后肺部免疫细胞的单细胞转录组分析
• GSE255794 表观转录组编辑筛选鉴定癌症中的功能性 m6A 位点 [ATAC-Seq]
• GSE255792 表观转录组编辑筛选鉴定癌症中的功能性 m6A 位点 [ChIP-Seq]
• GSE313336 CALD1/IGF2BP1 复合物通过以 m⁶A 依赖的方式增强 RFC4 mRNA 的稳定性来介导膀胱癌的顺铂耐药性。
• GSE312993 FlncKO-C2C12 肌源性分化过程中的转录组分析（批量 RNA 测序）
• GSE312903 Connectome-seq：通过条形码测序技术以单突触分辨率进行高通量神经元连接图谱绘制[snRNA-seq, ssRNA-seq]
• GSE307955 H526小细胞肺癌细胞中PAX5的ChIP-seq分析
• GSE301438 TopBP1 通过 PU.1 和 IRF8 控制传统树突状细胞发育和 Flt3L 驱动的 cDC1 分化 [ChIP-Seq]
• GSE271510 小鼠KPC胰腺肿瘤的单细胞RNA测序
• GSE271509 小鼠KPC胰腺肿瘤的单细胞ATAC测序
• GSE312168 组织损伤研究中转录组和代谢组变化的空间分辨整合分析
• GSE298302 西达米德和塞利尼索通过抑制 Survivin 和 PI3K/AKT 通路，在高级别 B 细胞淋巴瘤中发挥双重表观遗传和核输出抑制作用
• GSE286170 GABA能神经元在驱动脆性X染色体前突变携带者相关的CGG重复毒性中起关键作用[scRNA-Seq]
• GSE256000 原发性肾小管和透明细胞肾细胞癌细胞中开放染色质、HIF 结合和 mRNA 表达分析 [RNA-seq]
• GSE255998 原发性肾小管和 ccRCC 细胞中的开放染色质、HIF 结合和 mRNA 表达分析 [ATAC-seq]
• GSE255997 原发性肾小管和 ccRCC 细胞中开放染色质、HIF 结合和 mRNA 表达分析 [ChIP-seq]
• GSE313775 子宫内膜异位症患者和对照组的循环 Th1、Th1/17 和 Th17 细胞 RNA 测序
• GSE313636 对未感染或假结核耶尔森菌感染的回肠进行空间转录组分析，揭示了一种新的肠细胞转录状态
• GSE313324 PUF60介导的剪接是三阴性乳腺癌的关键驱动因素[CRISPR]
• GSE313289 通过基因表达了解头颈部鳞状细胞癌的肿瘤异质性
• GSE313117 基于基因表达的癌症分子分型跨平台比较揭示了与外显子捕获方法的差异
• GSE312890 表观转录组编辑筛选鉴定癌症中的功能性 m6A 位点 [CRISPR screen_H358]
• GSE309818 神经嵴细胞衍生的 DKK1 调节第二心场中的 Wnt 信号传导，从而协调心脏流出道发育。
• GSE305625 供体匹配血浆和脑脊液中多miRNA模型作为阿尔茨海默病预测候选生物标志物的比较
• GSE297746 自由漂浮的长期血管化间充质类器官 [RNA-seq]
• GSE297680 自由漂浮的长期血管化间充质类器官 [scRNA-seq]
• GSE295765 环状 ZBTB44 编码的 ZBTB44-342aa 通过抑制 cGAS-STING 通路减轻人主动脉瓣间质细胞的成骨分化
• GSE280899 PUF60介导的剪接是三阴性乳腺癌的关键驱动因素
• GSE280898 PUF60介导的剪接是三阴性乳腺癌的关键驱动因素[eCLIP]
• GSE268609 人类海马神经发生与成年、衰老和阿尔茨海默病的关系路线图
• GSE243490 人类嗜酸性粒细胞1型和2型的免疫极化
• GSE243369 小鼠嗜酸性粒细胞1型和2型的免疫极化
• GSE242447 表观转录组编辑筛选鉴定出癌症中的功能性 m6A 位点
• GSE242446 表观转录组编辑筛选鉴定癌症中的功能性 m6A 位点 [CRISPR screen_22Rv1]
• GSE242444 表观转录组编辑筛选鉴定癌症中的功能性 m6A 位点 [RNA-Seq_shKD]
• GSE313838 雄激素受体阴性前列腺癌易受SWI/SNF靶向降解分子的影响
• GSE313525 TAK1 是横纹肌肉瘤中致癌信号传导和分化阻滞的关键调节因子
• GSE313497 急性睡眠剥夺对小鼠海马和前额叶皮层非神经元细胞转录组特征的细胞特异性影响
• GSE313438 淫羊藿苷通过促进TFEB依赖性线粒体清除和代谢重编程来缓解运动引起的疲劳
• GSE313307 系统性感染改变阿尔茨海默病患者的皮质转录特征
• GSE312982 人类骨髓脂肪细胞通过脂质介导的血管生成素样蛋白4诱导促进前列腺癌骨转移进展
• GSE308383 小鼠肠神经系统的单核转录组分析
• GSE307220 Dgat1 通过解毒雄激素受体信号通路促进抗肿瘤 CD8+ T 细胞反应
• GSE301451 神经元偶像缺失可改善淀粉样蛋白病理
• GSE296719 铂(II)配合物抑制癌干细胞自我更新可抑制胃肠道癌转移
• GSE295408 靶向血清素驱动的组蛋白血清素化可抑制NEPC的谱系可塑性和进展
• GSE289577 NR5A2 的前馈调控环路确保植入前发育过程中基因的稳健激活 [RNA-seq]
• GSE289573 NR5A2 的前馈调控环路确保植入前发育过程中基因的稳健激活 [ATAC-seq]
• GSE124850 环状RNA在肝细胞癌肿瘤及肿瘤周围组织中的表达分析