详细内容（前10条）

1. ⭐ 最新8.5分单基因生信，泛癌+单细胞+空转+验证，多组学分析一起上分！

• ✍️ 作者：东晓生物
• 🏷️ 关键词：单细胞、基因组、生信
• 🔗 查看原文

2. ⭐ CellHint自动化协调与整合不同scRNA-seq数据集的细胞类型注释

• ✍️ 作者：生信菜鸟团
• 🏷️ 关键词：RNA-seq、RNAseq、scRNA
• 🔗 查看原文

3. 话进四 | Nature热议：AI会让生物信息失业嘛

• ✍️ 作者：单细胞天地
• 🏷️ 关键词：生物信息、生信
• 📝 描述：塞尔，你好。这是运来哥生信家书的第七十四封。一跨入四季度，时间带上了加速器，过得飞快。
• 🔗 查看原文

4. 免疫治疗的推动者–癌症疫苗

• ✍️ 作者：生信菜鸟团
• 🏷️ 关键词：癌症、免疫
• 🔗 查看原文

5. 7.7分1区生信，全文32个Figure聚焦最新热点：肿瘤神经浸润。小编认证的高质量生信文章，投稿到接收仅两个多月！

• ✍️ 作者：生信小课堂
• 🏷️ 关键词：肿瘤、生信
• 📝 描述：点击查看详情
• 🔗 查看原文

6. 艾伦脑科学研究所最新Nature，这个单细胞做的像树叶一样，生信代码学习

• ✍️ 作者：生信钱同学
• 🏷️ 关键词：单细胞、生信
• 📝 描述：nature单细胞文章分析学习
• 🔗 查看原文

7. Cell Stem Cell | 中科院曾安团队合作揭示幼年心脏再生关键细胞，为重启成年心脏再生提供全新靶点

• ✍️ 作者：转化医学网
• 🏷️ 关键词：TME、心脏
• 📝 描述：研究鉴定出幼年哺乳动物心脏再生过程中具有核心调控作用的损伤诱导型 Clusterin⁺（Clu⁺）心肌细胞亚群
• 🔗 查看原文

8. Cancer Cell：北京协和医学院肿瘤学领域新进展

• ✍️ 作者：丁香学术
• 🏷️ 关键词：肿瘤、cancer
• 🔗 查看原文

9. 单细胞hotspot分析基因模块识别

• ✍️ 作者：单细胞天地
• 🏷️ 关键词：单细胞
• 📝 描述：Hotspot主要是通过构建细胞的k-最近邻图，并在该图上进行局部自相关计算，从而发现细胞子集中具有协同表达关系的基因模块
• 🔗 查看原文

10. 详解：脂肪动员的代谢过程

• ✍️ 作者：小李代谢
• 🏷️ 关键词：代谢
• 📝 描述：脂肪动员是机体在需要能量时将储存的甘油三酯分解为游离脂肪酸和甘油并释放到血液中的生理过程。
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详细内容（前10条）

1. ⭐ GSE217531 基于转录组测序和网络药理学，探索青蒿琥酯干预胶质瘤细胞并产生不同治疗效果的潜在机制

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、oncology、carcinoma、immune、immunity、immunology、TME、cardiac、glioma、resistance、TCGA、DepMap、depmap、sequencing、RNAseq、single.cell、scRNA、scDNA、scATAC、spatial、spatially、Visium、genome、genomics、transcriptome、proteome、proteomics、Seurat、Scanpy、epigenetic、epigenome、histone、pathway、enrichment、clustering
• 📝 描述：Contributors : Tao Li ; Xia Mao ; Yanqiong Zhang ; Na LinSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensArtesunate possesses the potential of intervening with glioma, however, its pharmacological mechanisms remain unclarified. In this research, we aim to explore the regulatory mechanism of artesunate acting on glioma through constructing the interaction network of therapeutic effects-related genes of artesunate based on transcriptome sequencing data of glioma cells, and then verified by apoptosis staining and molecular docking at cellular level. In vitro cell activity and proliferation assay of two human glioma cell lines indicated that artesunate exerted more obvious inhibitory effects on the cell activity and proliferation of U87 cells than that of U251 cells. It could significantly promote apoptosis in U87 cells (P0.05), detected by using Hoechst and TUNEL cell apoptosis staining. Further, the differential expression gene sets between artesunate-sensitive and non-sensitive cell line, as well the therapeutic effects-related genes of artesunate were obtained, respectively, by transcriptome sequencing and differential data analysis using the lysis buffer of U87 and U251 cells before and after artesunate treatment. Then, key putative targets that related to therapeutic effects were screened by constructing the interaction network of differential genes of three above comparison groups, and calculating their topological characteristics. Pathway enrichment analysis showed that those key putative targets were significantly enriched in several signaling pathways that were closely associated with the main pathological changes of glioma, among which ATF4-DDIT3-PARP1 signaling axis, that was related with cell apoptosis, was the most enriched in. Molecular docking indicated that artesunate had fine binding affinities with ATF4 and DDIT3. Above all, this study preliminarily revealed that artesunate may induce the apoptosis and inhibited cell proliferation of glioma cells through regulating the abnormal alterations of corresponding proteins in ATF4-DDIT3-PARP1 signaling axis, which provided the novel scientific basis for its potential application in treating glioma.
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2. ⭐ GSE303537 大麻二酚发挥抗炎作用，但维持 T 效应记忆细胞分化：一项人类单细胞研究 [scRNA-seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、oncology、carcinoma、immune、immunity、immunology、TME、cardiac、glioma、resistance、TCGA、RNA-seq、RNAseq、DNA-seq、ATAC-seq、single.cell、single-cell、scRNA、scDNA、scATAC、genome、genomics、bioinformatics、Seurat、clustering
• 📝 描述：Contributors : Michael T Eadon ; Debora L GischSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCannabidiol is widely available and often used for pain management. Individuals with kidney disease or renal allografts have limited analgesia options. We conducted a Phase 1 healthy volunteer study to compare the peripheral immune cell distribution before (pre-cannabidiol) and after exposure to cannabidiol at steady state (post-cannabidiol). This study included specimens from 12 participants who received oral cannabidiol (up to 5 mg/kg twice daily) for 12 days. Lymphocytes were isolated and stimulated with anti-CD3/CD28 antibodies, with or without tacrolimus. Understanding the clinical safety of cannabidiol use is important given the paucity of pain control options available for immunocompromised transplant populations. Each individual has three conditions that are sequenced for the phase No CD3/CD28, CD3/CD28 and CD3/CD28 +Tacrolimus across two phases: pre-CBD and post-CBD.
• 🔗 查看原文

3. ⭐ GSE303068 大麻二酚发挥抗炎作用，但维持 T 效应记忆细胞分化：一项人类单细胞研究 [snRNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、oncology、carcinoma、immune、immunity、immunology、TME、cardiac、glioma、resistance、TCGA、RNA-seq、RNAseq、DNA-seq、ATAC-seq、single.cell、single-cell、scRNA、scDNA、scATAC、genome、genomics、bioinformatics、Seurat、clustering
• 📝 描述：Contributors : Michael T Eadon ; Debora L GischSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCannabidiol is widely available and often used for pain management. Individuals with kidney disease or renal allografts have limited analgesia options. We conducted a Phase 1 healthy volunteer study to compare the peripheral immune cell distribution before (pre-cannabidiol) and after exposure to cannabidiol at steady state (post-cannabidiol). This study included specimens from participants who received oral cannabidiol (up to 5 mg/kg twice daily) for 12 days. Lymphocytes were isolated and stimulated with anti-CD3/CD28 antibodies, with or without tacrolimus. Understanding the clinical safety of cannabidiol use is important given the paucity of pain control options available for immunocompromised transplant populations. Each individual has three conditions that are sequenced for the phase No CD3/CD28, CD3/CD28 and CD3/CD28 +Tacrolimus across two phases: pre-CBD and post-CBD.
• 🔗 查看原文

4. ⭐ GSE303503 EZH2 通过调节上皮细胞可塑性来指导乳腺癌进展 [RNA-Seq]

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、TME、cardiac、TCGA、DepMap、depmap、RNA-seq、RNAseq、DNA-seq、ChIP-seq、ATAC-seq、single.cell、scRNA、scDNA、scATAC、spatial、spatially、genome、genomics、Seurat、Scanpy、epigenetic、histone
• 📝 描述：Contributors : Linshan Liu ; Ellie Massey ; Dongmei Zuo ; Alain Pacis ; Mert Demirdizen ; Jessica Cinkornpumin ; Yu Gu ; Elizabeth Podleszanski ; Hailey Proud ; Virginie Sanguin-Gendreau ; Vasilios Papavasiliou ; Zhengze Jiang ; Harvey W. Smith ; William Pastor ; Paolo Ceppi ; William J. MullerSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBreast cancer remains a leading cause of death among women, encompassing diverse molecular subtypes with distinct clinical outcomes. The HER2+ subtype is particularly aggressive, with many patients eventually developing resistance to HER2-targeted therapies, underscoring the urgent need for alternative treatment strategies. Enhancer of Zeste Homolog 2 (EZH2), the catalytic subunit of Polycomb Repressive Complex 2, represses the expression of genetic programs crucial for differentiation, control of proliferation, and apoptosis through histone H3 lysine 27 trimethylation (H3K27me3). To investigate the role of EZH2 in HER2+ tumor progression, we crossed a genetically engineered mouse model (GEMM) of HER2-driven breast cancer with a conditional Ezh2 knockout strain. The results showed that Ezh2 is essential for accelerating tumor initiation and metastatic dissemination in HER2-driven breast cancer. Combined bulk and single cell RNA sequencing analyses revealed a significant downregulation of basal cell populations in the absence of Ezh2, characterized by the loss of Tp63 expression, and an upregulation of luminal progenitor cell populations, driven by crucial transcription factors such as Esr1, thereby driving luminal lineage commitment. Further, inhibition of EZH2 in vitro resulted in increased expression of ER in HER2+ human breast cancer cell lines and rendered them susceptible to tamoxifen. Altogether, these findings demonstrate that EZH2 dictates breast cancer plasticity through control of cellular identity and provides rationale for combining EZH2 inhibitors with endocrine therapies to improve outcomes in HER2+ breast cancer.
• 🔗 查看原文

5. ⭐ GSE303581 大麻二酚发挥抗炎作用，但维持T效应记忆细胞分化：一项人体单细胞研究

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、oncology、carcinoma、immune、immunity、immunology、TME、cardiac、glioma、resistance、TCGA、single.cell、single-cell、scRNA、scDNA、scATAC、genome、genomics、bioinformatics、Seurat、clustering
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
• 🔗 查看原文

6. ⭐ GSE277705 ISR 抑制通过增加少突胶质细胞存活率延长了佩利措伊斯-梅尔茨巴赫病小鼠模型的寿命

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、oncology、immune、TME、glioma、Alzheimer、TCGA、Hi-C、single.cell、single-cell、scRNA、scDNA、spatial、genome、genomics、proteome、proteomics、Seurat、histone、enrichment
• 📝 描述：Contributors : Brian Popko ; Yanan Chen ; Rejani Kunjamma ; Karin Lin ; Caitlin Connellly ; Carmela SidrauskiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe leukodystrophy Pelizaeus-Merzbacher disease (PMD) is caused by myelin protein proteolipid protein (PLP) gene mutations. PMD is characterized by oligodendrocyte death and CNS hypomyelination; thus, increasing oligodendrocyte survival and enhancing myelination could provide therapeutic benefit. We used the PMD mouse model Jimpy to explore the impact of the integrated stress response (ISR) on the oligodendrocyte response to mutant PLP expression. Jimpy animals in which the ISR-triggering eIF2 kinase PERK is inactivated have an extended lifespan that correlates with increased oligodendrocyte survival and enhanced CNS myelination. Inactivation of the downstream components of the ISR pathway CHOP and GADD34, in contrast, did not rescue oligodendrocytes or myelin. Phosphorylated eIF2 inhibits the exchange factor eIF2B, resulting in diminished protein synthesis. Treatment with small molecule eIF2B activators 2BAct and ISRIB dramatically increased oligodendrocyte survival, CNS myelination, and doubled the lifespan of Jimpy mice. These results suggest that ISR modulation could provide therapeutic benefit to PMD patients.
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7. ⭐ GSE288522 单细胞 (sc) RNA测序揭示了NSG肿瘤模型中转基因MAGE-A1特异性TCR-T CD4+和CD8+细胞的转录状态

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、carcinoma、TME、glioma、resistance、TCGA、RNAseq、single.cell、single-cell、scRNA、scATAC、spatial、Visium、genome、genomics、transcriptome、transcriptomics、epigenome
• 📝 描述：Contributors : Shihong Zhang ; Tzu-Hao Tang ; Sinead Kinsella ; Francesco Mazziotta ; Lauren Martin ; Bo Lee ; Aude ChapuisSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe have studied the efficacy of MAGE-A1 specific transgenic T cells against A375F NSG model by flow cytometry and IHC analysis. We then employed a scRNAseq approach to characterize the transcriptional states of transgenic CD4+ and CD8+ TCR-T cells that infiltrated into A375F tumor by extracting these cells from tumors. This scRNAseq study allow us to compare the transcriptional differences between our groups ( T-TCR-MA1-CD8ab, T-TCR-MA1-CD8/28)
• 🔗 查看原文

8. ⭐ GSE297659 人类体外肿瘤相关巨噬细胞模型的表征及其转化相关性

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、carcinoma、immune、TME、cardiac、resistance、TCGA、Hi-C、scRNA、scDNA、scATAC、spatial、genome、genomics、Seurat、histone、enrichment
• 📝 描述：Contributors : Arthur Dyer ; Rebecca Dudley ; Shreya Ahuja ; Clara Alsinet ; Pawan Poudel ; Georgina Bowyer ; Kalvin Sahota ; Saly Songvilay ; Des Jones ; Matthew Glover ; Sonja Hess ; Elina Timosenko ; Simon J DovediSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTumour-associated macrophages (TAMs) are key components of the tumour microenvironment with a demonstrated ability to modulate anti-tumour T-cell responses and immunotherapy outcomes. With increasing realisation that the M1/M2 paradigm does not reflect the complexity of macrophage phenotypes in cancer patients, an urgent need has arisen to develop improved, translatable in vitro models for human TAMs. To address this gap, we have screened conditioned media from a panel of tumour cell lines for their ability to induce suppressive marker upregulation on human monocyte-derived macrophages (hMDMs), as well as active T-cell immunosuppression. We performed secretome characterization of these tumour-conditioned media (TCM) to shed light on cancer cell-derived soluble factors that may contribute to TAM polarisation. Furthermore, we characterized the proteomic and transcriptomic signatures of macrophages exposed to either TCM or primary ascites fluid from ovarian cancer patients and performed bioinformatics analysis to determine the most translationally relevant models of TAMs. In summary, our work provides mechanistic insights on tumour-macrophage crosstalk in the context of establishing suppressive TAM phenotypes and addresses the long-standing gap of defining translationally relevant human in vitro TAM models.
• 🔗 查看原文

9. ⭐ GSE308064 PAD4⁺ 中性粒细胞通过 NETs-DNA/TAOK1/MAPK 通路促进肝星状细胞活化并加速 MASH 纤维化进展

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、TME、glioma、TCGA、DepMap、depmap、scRNA、scDNA、scATAC、spatial、spatially、genome、genomics、proteome、Seurat、Scanpy、histone、pathway
• 📝 描述：Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensNeutrophils play a pivotal role in the progression of metabolic-associated steatohepatitis (MASH) by mediating inflammatory responses. However, the heterogeneity of neutrophil subsets in MASH and their specific contributions to disease progression remain unclear. In this study, analysis of liver biopsies from 265 patients revealed a strong association between elevated neutrophil counts and MASH severity, particularly fibrosis. Five distinct neutrophil subsets were identified in human liver tissue, with PAD4⁺ neutrophils serving as key drivers in MASH progression. Mechanistically, PAD4⁺ neutrophils generate neutrophil extracellular traps (NETs) and activate hepatic stellate cells via the TAOK1-dependent MAPK signaling pathway. Inhibition of PAD4⁺ neutrophils in vivo attenuated the progression of liver fibrosis without exacerbating liver injury. Collectively, these findings elucidate the pivotal involvement of PAD4⁺ neutrophils in MASH progression and identify them as promising therapeutic targets for mitigating fibrosis and inflammation.
• 🔗 查看原文

10. ⭐ GSE305815 玉米灰斑病早期、中期和晚期玉米尾孢菌转录组的分析

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、oncology、carcinoma、TME、glioma、TCGA、scRNA、scDNA、scATAC、spatial、spatially、genome、transcriptome、proteome、Scanpy、methylation、histone
• 📝 描述：Contributors : Trystan R Nadasen ; Ingo Hein ; Dave K BergerSeries Type : Expression profiling by high throughput sequencingOrganism : Cercospora zeina ; Zea maysCercospora zeina is a fungal pathogen that causes gray leaf spot (GLS) disease on maize (Zea mays L.) in South Africa. Upon landing on a maize leaf, the pathogen rapidly enters through the stomata and continues to develop asymptomatically for up to 28 days before symptoms are seen. As previous work has yet to adequately determine how the pathogen behaves during its infective period, we used transcriptomics to gain insights about the in-planta development of the pathogen and explore how it uses its effectors to facilitate this process. Samples from B73 maize inbreds infected with C. zeina were harvested in a time course experiment and used for RNA sequencing. We used reads mapped to the C. zeina genome as a proxy for biomass accumulation. At the end of the latent period, C. zeina was found to rapidly accumulate biomass and showed a nearly 50-fold increase in biomass as symptoms appeared. There were two distinct transcriptional waves occurring across the infection period. The first wave showed expression of genes for cellular growth, maintenance and immune avoidance, whereas the second wave was enriched with genes involved in detoxification and carbohydrate catabolism. A total of 140 putative effector genes were differentially expressed over the time-courses, with most up-regulated during the mid stage when the switch to necrotrophy occurs. Transient expression of three of these C. zeina effectors (CzEcp2, CzNis1a, CzNis1b) induced plant immunity in Nicotiana spp. resulting in the development of a hypersensitive response. This suggests that a cohort of C. zeina effectors expressed at this time have functions for which receptors have evolved in non-host species like tobacco. Altogether, this work suggests C. zeina behaves as a latent necrotroph during infection and provides a foundation for future research into the infection biology of C. zeina on maize.
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1. ⭐ QIAGEN将收购Parse Biosciences，从而将其样品技术产品组合扩展至高度可扩展的单细胞解决方案领域。

• ✍️ 作者：未知作者
• 🏷️ 关键词：tumor、cancer、oncology、TME、cardiac、resistance、TCGA、Hi-C、single.cell、single-cell、scRNA、scDNA、scATAC、spatial、genome、genomics、Scanpy、epigenetic、methylation、histone
• 📝 描述：QIAGEN’s acquisition of Parse Biosciences expands its RNA sequencing and single-cell analysis capabilities, fueling AI-powered drug discovery through scalable, instrument-free…
• 🔗 查看原文

2. ⭐ 单细胞RNA测序揭示藏猪脂肪生成和脂肪酸转运增强

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、cardiac、TCGA、sequencing、RNA-seq、RNAseq、DNA-seq、ATAC-seq、Hi-C、single.cell、single-cell、scRNA、scDNA、scATAC、Scanpy、histone
• 📝 描述：Using single-cell RNA sequencing, researchers uncovered enhanced adipogenesis and fatty acid transport in Tibetan pigs, explaining their superior fat accumulation and cold adaptation compared to Duroc pigs…
• 🔗 查看原文

3. ⭐ 阐明一种调节骨骼生长的新机制

• ✍️ 作者：未知作者
• 🏷️ 关键词：cancer、immune、TME、TCGA、histone
• 📝 描述：RNA sequencing revealed that Dnmt1 controls energy metabolism in growth plate chondrocytes, ensuring proper bone elongation and offering insight into bone growth disorders…
• 🔗 查看原文

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• GSE279824 人类腺样体单细胞转录组分析揭示气道微褶皱细胞的分子特征
• GSE248477 利用牛宿主转录组学评估市场和疫苗如何影响健康和疾病
• GSE289792 抑制小胶质细胞 SLC2A5 可减轻急性缺血性中风后的脑损伤 [RNA-Seq]
• GSE289791 抑制小胶质细胞 SLC2A5 可减轻急性缺血性中风后的脑损伤 [scRNA-Seq]
• GSE217966 缬氨酸通过改变小鼠自噬和核酸甲基化诱导睾丸细胞凋亡
• GSE297607 RhCMV扩增CCR5记忆T细胞并促进SIV在肠道黏膜中的储存库形成
• GSE276305 UMP 作为 NR4A1 的内源性调节因子，控制胃癌的进展 [ChIP-Seq]
• GSE141250：24-28周龄野生型和ApoE-/-小鼠外膜和中膜/内膜细胞的单细胞RNA测序
• GSE292813 UGT2B17的非经典功能促进去势抵抗性前列腺癌的进展
• GSE140415 OncoScan CNV 检测（Thermo Fisher Scientific/Affymetrix）数据，包含 13 个冷凝集素病样本
• GSE300036 老年小鼠白色脂肪组织的单细胞RNA测序（sc RNAseq）
• GSE293218 秀丽隐杆线虫长寿干预的有效性取决于RNA剪接因子II的早期生命活性
• GSE292723 秀丽隐杆线虫长寿干预的有效性取决于RNA剪接因子的早期生命活性
• GSE309530 RNA-seq 分析：骨折手术和 Protectin DX 治疗后背根神经节 (DRG) 的 RNA 测序谱
• GSE263680 受体激酶异二聚体精细调控拟南芥形成层活性
• GSE276294 UMP作为NR4A1的内源性调节因子，调控胃癌的进展
• GSE286551 甲基-CpG结合蛋白2抑制cGAS相关信号传导
• GSE298388 髓系 FABP5 对炎症性疼痛的调节
• GSE140534 人类小胶质细胞的存活因素